LOCUS       sequence-43756-e         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Subach OM, Entenberg D, Condeelis JS, Verkhusha VV
  TITLE     A FRET-facilitated photoswitching using an orange fluorescent 
            protein with the fast photoconversion kinetics.
  JOURNAL   J Am Chem Soc. 2012 Aug 17.
  PUBMED    22900938
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc. 2012 Aug 17."
COMMENT     Sequence Label: pH2B-PSmOrange2
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             91..468
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      481..490
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctggtttagt gaaccgtcag atccgctagc gctaccggac tcagatctcg agctcaagct
       61 tcgaattctg cagtcgacgg taccgccacc atgccagagc cagcgaagtc tgctcccgcc
      121 ccgaaaaagg gctccaagaa ggcggtgact aaggcgcaga agaaaggcgg caagaagcgc
      181 aagcgcagcc gcaaggagag ctattccatc tatgtgtaca aggttctgaa gcaggtccac
      241 cctgacaccg gcatttcgtc caaggccatg ggcatcatga attcgtttgt gaacgacatt
      301 ttcgagcgca tcgcaggtga ggcttcccgc ctggcgcatt acaacaagcg ctcgaccatc
      361 acctccaggg agatccagac ggccgtgcgc ctgctgctgc ctggggagtt ggccaagcac
      421 gccgtgtccg agggtactaa ggccatcacc aagtacacca gcgctaagga tccaccggtc
      481 gccaccatgg tgagcaaggg cgaggagaat aatatggcca tcattaagga gttcatgcgc
      541 ttcaaggtgc acatggaggg cactgtgaac ggccacgagt tcgagatcga gggcgagggc
      601 gagggccacc cctacgaggg ctttcagacc gctaagctga aggtgaccaa gggcggcccc
      661 ctgcccttcg cctgggacat cctgtcccct ctcatcacct acggctccaa ggcctacgtg
      721 aagcaccccg ccgacatccc cgactacttc aagctgtcct tccccgaggg cttcaagtgg
      781 gagcgcgtga tgaactacga ggacggcggc gtggtgaccg tgacccagga ctcctctctg
      841 caggacggcg agttcatcta caaggtgaag atgcgcggca ccaacttccc
//
