LOCUS       sequence-437566-        4412 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4412)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 4412)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pEntry_HCoV-OC43-nsp14
FEATURES             Location/Qualifiers
     source          1..4412
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     435..454
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     688..705
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(864..891)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(983..1069)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1118..1140
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1132..1149
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1133..1149
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1165..1264
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2887..2986)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3003..3022)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3004..3022)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3027..3043)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3027..3043)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3474..4265
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(4358..4412,1..534)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
       61 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
      121 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
      181 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
      241 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
      301 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
      361 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
      421 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
      481 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
      541 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
      601 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
      661 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
      721 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
      781 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc
      841 caggaagagt ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt
      901 gatgcctggc agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa
      961 cgttcaaatc cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac
     1021 agataaaacg aaaggcccag tcttccgact gagcctttcg ttttatttga tgcctggcag
     1081 ttccctactc tcgcgttaac gctagcatgg atgttttccc agtcacgacg ttgtaaaacg
     1141 acggccagtc ttaagctcgg gccccaaata atgattttat tttgactgat agtgacctgt
     1201 tcgttgcaac aaattgatga gcaatgcttt tttataatgc caactttgta caaaaaagca
     1261 gaagggccgt caaggccagg atccatggga tcctgtagta ctaatttatt taaagattgt
     1321 agcaagagtt atagcggtta tcacccagct catgctcctt catttttggc agtagatgac
     1381 aaatataagg caactggcga tttagccgtg tgtcttggta ttggtgattc tgctgttaca
     1441 tattcaagat taatatcact catgggtttt aaattggatg ttacccttga tgggtattgt
     1501 aagcttttta taactaaaga agaagctgtt aaacgcgtgc gtgcctgggt tggctttgat
     1561 gctgaaggtg ctcatgccac gcgtgatagc attgggacaa atttcccact tcaattagga
     1621 ttttccacag gaattgattt tgttgtggaa gccactggtt tgtttgctga tagagatggt
     1681 tacagcttta aaaaggctgt ggcgaaagct cctcctggtg aacaatttaa gcacctcatc
     1741 cctttgatga cgagaggtca tcgctgggat gttgttagac ctagaatagt acaaatgttt
     1801 gcagatcatt taattgatct gtctgattgt gttgtgctag ttacatgggc agccaacttt
     1861 gagctcactt gtctccgcta ctttgcaaaa gtagggcgtg agatttcttg taatgtatgc
     1921 actaaacgtg ccacagttta caattctaga actggttact atggttgttg gcgccatagt
     1981 gttacatgtg attacttgta taatccactt attgttgata ttcaacagtg gggatatatt
     2041 ggttctttat caagtaatca tgatttatat tgtagtgtcc ataaaggagc acatgttgct
     2101 tcctctgatg ctataatgac acggtgtttg gccgtttatg attgcttttg caataatatt
     2161 aattggaatg tggagtatcc catcatttca aatgagttaa gtattaatac ctcttgtagg
     2221 gtcttgcagc gtgtgattct taaagctgcc atgctctgca acagatatac tttgtgttat
     2281 gatattggca acccaaaagc gattgcctgt gtcaaagatt ttgattttaa gttctatgat
     2341 gcccaaccaa ttgttaagtc tgttaagact cttttgtatt cttttgaggc acataaggac
     2401 tcttttaaag acggtttgtg tatgttttgg aactgtaatg tggataagta tccaccgaat
     2461 gcagttgtat gtagatttga cactagagtg ttgaataatt taaatcttcc tggctgtaat
     2521 ggaggtagtt tgtatgttaa taaacatgca ttccacacta aaccctttgc tagggcagcc
     2581 tttgagcatt tgaagcctat gccattcttc tattattcag atacgccttg tgtgtatatg
     2641 gatggcatgg atgctaagca ggttgattat gtacctttga aatctgccac gtgcatcaca
     2701 agatgcaatt taggtggtgc agtttgttta aaacatgctg aagagtatcg tgagtactta
     2761 gagtcttaca atacagctac tacagcaggt tttacttttt gggtctataa gacatttgat
     2821 ttttataatt tgtggaatac gttcaccaag ctacaactta attaagacgc gatcgcggcc
     2881 tcatgggccc agctttcttg tacaaagttg gcattataaa aaataattgc tcatcaattt
     2941 gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgata
     3001 tcccctatag tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt
     3061 ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatgc ctcctctaga
     3121 ccagccagga cagaaatgcc tcgacttcgc tgctacccaa ggttgccggg tgacgcacac
     3181 cgtggaaacg gatgaaggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt
     3241 aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg
     3301 gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta
     3361 tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga
     3421 gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac attatgaggg
     3481 aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc
     3541 atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga
     3601 agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc
     3661 ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc
     3721 tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag
     3781 ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg
     3841 agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg
     3901 ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat
     3961 ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg
     4021 agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg
     4081 cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg
     4141 tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg
     4201 cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca
     4261 aataaccctc gagccaccca tgaccaaaat cccttaacgt gagttacgcg tcgttccact
     4321 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     4381 taatctgctg cttgcaaaca aaaaaaccac cg
//
