LOCUS       sequence-437589-        8444 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  equimolar expression of mBaojin and mCherry in budding yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8444)
  AUTHORS   Botman D, de Groot DH, Wehrens M, van Rossum K, Yska S, van der 
            Kieft B, Albert V, Minghetti N, Goedhart J, Teusink B
  TITLE     Side-by-side systematic characterization of novel FPs in budding 
            yeast
  JOURNAL   bioRxiv 2025.06.27.661975; doi: 
            https://doi.org/10.1101/2025.06.27.661975
REFERENCE   2  (bases 1 to 8444)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.06.27.661975; doi: https://doi.org/10.1101/2025.06.27.661975"
COMMENT     Sequence Label: pDRF1 mBaojin-T2A-mCherry
FEATURES             Location/Qualifiers
     source          1..8444
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    800..824
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             831..1532
                     /codon_start=1
                     /product="bright and photostable monomeric green 
                     fluorescent protein derived from StayGold (Zhang et al., 
                     2024)"
                     /label=mBaoJin
                     /label=mStayGold
                     /note="/vntifkey=21"
                     /translation="MVSKGEEENMASTPFKFQLKGTINGKSFTVEGEGEGNSHEGSHKG
                     KYVCTSGKLPMSWAALGTTFGYGMKYYTKYPSGLKNWFREVMPGGFTYDRHIQYKGDGS
                     IHAKHQHFMKNGTYHNIVEFTGQDFKENSPVLTGDMNVSLPNEVPQIPRDDGVECPVTL
                     LYPLLSDKSKYVEAHQYTICKPLHNQPAPDVPYHWIRKQYTQSKDDAEERDHICQSETL
                     EAHLKGMDELYK"
     CDS             1539..1592
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      1608..1617
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1614..2324
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1759..1777)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2027..2046
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(2334..2358)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(2420..2439)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2421..2439)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      2587..2774
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2642..2666)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(2786..2803)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2786..2802)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2795..2817)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3238..4584
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(4695..5498)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(5499..5714)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(5739..5757)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5825..5929
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5930..5998,5999..6790)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6148..6167)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6961..7549
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7450..7469
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7703..7720
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7837..7858
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7873..7878,7879..7896,7897..7903)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7911..7927
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7916..7938
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7935..7951
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7935..7951
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(7981..8436)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8118..8139)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8330..8349
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 caagcttcct gaaacggaga aacataaaca ggcattgctg ggatcaccca tacatcactc
       61 tgttttgcct gaccttttcc ggtaatttga aaacaaaccc ggtctcgaag cggagatccg
      121 gcgataatta ccgcagaaat aaacccatac acgagacgta gaaccagccg cacatggccg
      181 gagaaactcc tgcgagaatt tcgtaaactc gcgcgcattg catctgtatt tcctaatgcg
      241 gcacttccag gcctcgatcg agaccgttta tccattgctt ttttgttgtc tttttccctc
      301 gttcacagaa agtctgaaga agctatagta gaactatgag ctttttttgt ttctgttttc
      361 cttttttttt tttttacctc tgtggaaatt gttactctca cactctttag ttcgtttgtt
      421 tgttttgttt attccaatta tgaccggtga cgaaacgtgg tcgatggtgg gtaccgctta
      481 tgctcccctc cattagtttc gattatataa aaaggccaaa tattgtatta ttttcaaatg
      541 tcctatcatt atcgtctaac atctaatttc tcttaaattt tttctctttc tttcctataa
      601 caccaatagt gaaaatcttt ttttcttcta tatctacaaa aacttttttt ttctatcaac
      661 ctcgttgata aattttttct ttaacaatcg ttaataatta attaattgga aaataaccat
      721 tttttctctc ttttatacac acattcaaaa gaaagaaaaa aaatataccc cagcctcgac
      781 tagtgaattc gcggccatca caagtttgta caaaaaagca ggctgctagc atggtctcaa
      841 agggagagga ggaaaacatg gctagtacac catttaaatt tcaacttaaa ggaaccatca
      901 atggcaaatc gtttaccgtt gaaggcgaag gtgaagggaa ctcacatgaa ggttctcata
      961 aaggaaaata tgtttgtaca agtggaaaac taccgatgtc atgggcagca cttgggacaa
     1021 cctttggtta tggaatgaaa tattatacca aatatcctag tggactgaag aactggtttc
     1081 gtgaagtaat gcccggaggc tttacctacg atcgtcatat tcaatataaa ggcgatggga
     1141 gtatccatgc aaaacaccaa cactttatga aaaatgggac ttatcacaac attgtagaat
     1201 ttaccggtca ggattttaaa gaaaatagtc cagtcttaac tggagatatg aatgtctcat
     1261 taccgaatga agtcccacaa atacccagag atgatggagt agaatgccca gtgaccttgc
     1321 tttatccttt attatcggat aaatcaaaat acgtcgaggc tcaccaatat acaatctgca
     1381 agcctcttca taatcaacca gcacctgatg tcccatatca ctggattcgt aaacaataca
     1441 cacaaagcaa agatgatgcc gaggaacgcg atcatatctg tcaatcagag actctcgaag
     1501 cacacttaaa gggcatggac gagctgtata agtccggaga gggcagagga agtcttctaa
     1561 catgcggtga cgtggaggag aatcccggcc ctggggatcc accggtcgcc accatggtga
     1621 gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca
     1681 tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct
     1741 acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct
     1801 gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg
     1861 acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga
     1921 acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt
     1981 tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga
     2041 agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga
     2101 agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca
     2161 agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca
     2221 agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg
     2281 agggccgcca ctccaccggc ggcatggacg agctgtacaa gtaaagcggc cgcacccagc
     2341 tttcttgtac aaagtggtgt agttaattca tgatggccgc tgcaggtcga cctcgagggg
     2401 gggcccggta cccaattcgc cctatagtga gtcgtattac gcgcggatcc agctttggac
     2461 ttcttcgcca gaggtttggt caagtctcca atcaaggttg tcggcttgtc taccttgcca
     2521 gaaatttacg aaaagatgga aaagggtcaa atcgttggta gatacgttgt tgacacttct
     2581 aaataagcga atttcttatg atttatgatt tttattatta aataagttat aaaaaaaata
     2641 agtgtataca aattttaaag tgactcttag gttttaaaac gaaaattctt attcttgagt
     2701 aactctttcc tgtaggtcag gttgctttct caggtatagc atgaggtcgc tcttattgac
     2761 cacacctcta ccggcatgcc aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     2821 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     2881 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     2941 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcataatcg
     3001 gatcgtactt gttacccatc attgaatttt gaacatccga acctggaagt tttccctgaa
     3061 acagatagta tatttgaacc tgtataataa tatatagtct agcgctttac ggaagacaat
     3121 gtatgtattt cggttcctgg agaaactatt gcatctattg cataggtaat cttgcacgtc
     3181 gcatccccgg ttcattttct gcgtttccat cttgcacttc aatagcatat ctttgttaac
     3241 gaagcatctg tgcttcattt tgtagaacaa aaatgcaacg cgagagcgct aatttttcaa
     3301 acaaagaatc tgagctgcat ttttacagaa cagaaatgca acgcgaaagc gctattttac
     3361 caacgaagaa tctgtgcttc atttttgtaa aacaaaaatg caacgcgaga gcgctaattt
     3421 ttcaaacaaa gaatctgagc tgcattttta cagaacagaa atgcaacgcg agagcgctat
     3481 tttaccaaca aagaatctat acttcttttt tgttctacaa aaatgcatcc cgagagcgct
     3541 atttttctaa caaagcatct tagattactt tttttctcct ttgtgcgctc tataatgcag
     3601 tctcttgata actttttgca ctgtaggtcc gttaaggtta gaagaaggct actttggtgt
     3661 ctattttctc ttccataaaa aaagcctgac tccacttccc gcgtttactg attactagcg
     3721 aagctgcggg tgcatttttt caagataaag gcatccccga ttatattcta taccgatgtg
     3781 gattgcgcat actttgtgaa cagaaagtga tagcgttgat gattcttcat tggtcagaaa
     3841 attatgaacg gtttcttcta ttttgtctct atatactacg tataggaaat gtttacattt
     3901 tcgtattgtt ttcgattcac tctatgaata gttcttacta caattttttt gtctaaagag
     3961 taatactaga gataaacata aaaaatgtag aggtcgagtt tagatgcaag ttcaaggagc
     4021 gaaaggtgga tgggtaggtt atatagggat atagcacaga gatatatagc aaagagatac
     4081 ttttgagcaa tgtttgtgga agcggtattc gcaatatttt agtagctcgt tacagtccgg
     4141 tgcgtttttg gttttttgaa agtgcgtctt cagagcgctt ttggttttca aaagcgctct
     4201 gaagttccta tactttctag ctagagaata ggaacttcgg aataggaact tcaaagcgtt
     4261 tccgaaaacg agcgcttccg aaaatgcaac gcgagctgcg cacatacagc tcactgttca
     4321 cgtcgcacct atatctgcgt gttgcctgta tatatatata catgagaaga acggcatagt
     4381 gcgtgtttat gcttaaatgc gtacttatat gcgtctattt atgtaggatg aaaggtagtc
     4441 tagtacctcc tgtgatatta tcccattcca tgcggggtat cgtatgcttc cttcagcact
     4501 accctttagc tgttctatat gctgccactc ctcaattgga ttagtctcat ccttcaatgc
     4561 tatcatttcc tttgatattg gatcgatccg atgataagct gtcaaacatg agaattgggt
     4621 aataactgat ataattaaat tgaagctcta atttgtgagt ttagtataca tgcatttact
     4681 tataatacag ttttttagtt ttgctggccg catcttctca aatatgcttc ccagcctgct
     4741 tttctgtaac gttcaccctc taccttagca tcccttccct ttgcaaatag tcctcttcca
     4801 acaataataa tgtcagatcc tgtagagacc acatcatcca cggttctata ctgttgaccc
     4861 aatgcgtctc ccttgtcatc taaacccaca ccgggtgtca taatcaacca atcgtaacct
     4921 tcatctcttc cacccatgtc tctttgagca ataaagccga taacaaaatc tttgtcgctc
     4981 ttcgcaatgt caacagtacc cttagtatat tctccagtag atagggagcc cttgcatgac
     5041 aattctgcta acatcaaaag gcctctaggt tcctttgtta cttcttctgc cgcctgcttc
     5101 aaaccgctaa caatacctgg gcccaccaca ccgtgtgcat tcgtaatgtc tgcccattct
     5161 gctattctgt atacacccgc agagtactgc aatttgactg tattaccaat gtcagcaaat
     5221 tttctgtctt cgaagagtaa aaaattgtac ttggcggata atgcctttag cggcttaact
     5281 gtgccctcca tggaaaaatc agtcaagata tccacatgtg tttttagtaa acaaattttg
     5341 ggacctaatg cttcaactaa ctccagtaat tccttggtgg tacgaacatc caatgaagca
     5401 cacaagtttg tttgcttttc gtgcatgata ttaaatagct tggcagcaac aggactagga
     5461 tgagtagcag cacgttcctt atatgtagct ttcgacatga tttatcttcg tttcctgcat
     5521 gtttttgttc tgtgcagttg ggttaagaat actgggcaat ttcatgtttc ttcaacacta
     5581 catatgcgta tatataccaa tctaagtctg tgctccttcc ttcgttcttc cttctgttcg
     5641 gagattaccg aatcaaaaaa atttcaagga aaccgaaatc aaaaaaaaga ataaaaaaaa
     5701 aatgatgaat tgaaaagcta attcttgaag acgaaagggc ctcgtgatac gcctattttt
     5761 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     5821 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     5881 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     5941 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     6001 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     6061 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     6121 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     6181 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     6241 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     6301 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     6361 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     6421 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     6481 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     6541 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     6601 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     6661 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     6721 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     6781 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     6841 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     6901 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     6961 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     7021 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     7081 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     7141 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     7201 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     7261 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     7321 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     7381 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     7441 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     7501 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     7561 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     7621 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     7681 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     7741 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     7801 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     7861 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     7921 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctta ccgcatcagg
     7981 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
     8041 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
     8101 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
     8161 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
     8221 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
     8281 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
     8341 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
     8401 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccat tcgc
//
