LOCUS       sequence-437592-        8447 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  equimolar expression of mScarlet1.4 and mTurquoise2 in budding 
            yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8447)
  AUTHORS   Botman D, de Groot DH, Wehrens M, van Rossum K, Yska S, van der 
            Kieft B, Albert V, Minghetti N, Goedhart J, Teusink B
  TITLE     Side-by-side systematic characterization of novel FPs in budding 
            yeast
  JOURNAL   bioRxiv 2025.06.27.661975; doi: 
            https://doi.org/10.1101/2025.06.27.661975
REFERENCE   2  (bases 1 to 8447)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.06.27.661975; doi: https://doi.org/10.1101/2025.06.27.661975"
COMMENT     Sequence Label: pDRF1 mScarlet1.4-T2A-mTurquoise2
FEATURES             Location/Qualifiers
     source          1..8447
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    800..824
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             831..1526
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFTWERVMNFEDGG
                     SVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKTTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYIADFKTTYKAKKPVQMPGAYNVDRKLDIVSHNEDYTVVEQYEASVGRH
                     STGGMDELYK"
     primer_bind     complement(964..982)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             1533..1586
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      1602..1611
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1608..1610,1611..1613,1614..2327)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1653..1674)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1914..1933)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2261..2282
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(2337..2361)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(2423..2442)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2424..2442)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      2590..2777
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2645..2669)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(2789..2806)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2789..2805)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2798..2820)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3241..4587
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(4698..5501)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(5502..5717)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(5742..5760)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5828..5932
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5933..6001,6002..6793)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6151..6170)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6964..7552
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7453..7472
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7706..7723
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7840..7861
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7876..7881,7882..7899,7900..7906)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7914..7930
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7919..7941
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7938..7954
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7938..7954
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(7984..8439)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8121..8142)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8333..8352
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 caagcttcct gaaacggaga aacataaaca ggcattgctg ggatcaccca tacatcactc
       61 tgttttgcct gaccttttcc ggtaatttga aaacaaaccc ggtctcgaag cggagatccg
      121 gcgataatta ccgcagaaat aaacccatac acgagacgta gaaccagccg cacatggccg
      181 gagaaactcc tgcgagaatt tcgtaaactc gcgcgcattg catctgtatt tcctaatgcg
      241 gcacttccag gcctcgatcg agaccgttta tccattgctt ttttgttgtc tttttccctc
      301 gttcacagaa agtctgaaga agctatagta gaactatgag ctttttttgt ttctgttttc
      361 cttttttttt tttttacctc tgtggaaatt gttactctca cactctttag ttcgtttgtt
      421 tgttttgttt attccaatta tgaccggtga cgaaacgtgg tcgatggtgg gtaccgctta
      481 tgctcccctc cattagtttc gattatataa aaaggccaaa tattgtatta ttttcaaatg
      541 tcctatcatt atcgtctaac atctaatttc tcttaaattt tttctctttc tttcctataa
      601 caccaatagt gaaaatcttt ttttcttcta tatctacaaa aacttttttt ttctatcaac
      661 ctcgttgata aattttttct ttaacaatcg ttaataatta attaattgga aaataaccat
      721 tttttctctc ttttatacac acattcaaaa gaaagaaaaa aaatataccc cagcctcgac
      781 tagtgaattc gcggccatca caagtttgta caaaaaagca ggctgctagc atggtgagca
      841 agggcgaggc agtgatcaag gagttcatgc ggttcaaggt gcacatggag ggctccgtga
      901 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
      961 ccgccaagct gaaggtgacc aagggtggcc ccctgccctt ctcctgggac atcctgtccc
     1021 ctcagttcat gtacggctcc agggccttca ccaagcaccc cgccgacatc cccgactact
     1081 ataagcagtc cttccccgag ggcttcacct gggagcgcgt gatgaacttc gaggacggcg
     1141 gctccgtgac cgtgacccag gacacctccc tggaggacgg caccctgatc tacaaggtga
     1201 agctccgcgg caccaacttc cctcctgacg gccccgtaat gcagaagaca acaatgggct
     1261 gggaagcgtc caccgagcgg ttgtaccccg aggacggcgt gctgaagggc gacattaaga
     1321 tggccctgcg cctgaaggac ggcggccgct acatcgcgga cttcaagacc acctacaagg
     1381 ccaagaagcc cgtgcagatg cccggcgcct acaacgtcga ccgcaagttg gacatcgtct
     1441 cccacaacga ggactacacc gtggtggaac agtacgaagc ctccgtgggc cgccactcca
     1501 ccggcggcat ggacgagctg tacaagtccg gagagggcag aggaagtctt ctaacatgcg
     1561 gtgacgtgga ggagaatccc ggccctgggg atccaccggt cgccaccatg gtgagcaagg
     1621 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     1681 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     1741 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     1801 tgtcctgggg cgtgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct
     1861 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     1921 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     1981 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     2041 actactttag cgacaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggcca
     2101 acttcaagat ccgccacaac atcgaggacg gcggcgtgca gctcgccgac cactaccagc
     2161 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     2221 agtccaagct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     2281 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaaagc ggccgcaccc
     2341 agctttcttg tacaaagtgg tgtagttaat tcatgatggc cgctgcaggt cgacctcgag
     2401 ggggggcccg gtacccaatt cgccctatag tgagtcgtat tacgcgcgga tccagctttg
     2461 gacttcttcg ccagaggttt ggtcaagtct ccaatcaagg ttgtcggctt gtctaccttg
     2521 ccagaaattt acgaaaagat ggaaaagggt caaatcgttg gtagatacgt tgttgacact
     2581 tctaaataag cgaatttctt atgatttatg atttttatta ttaaataagt tataaaaaaa
     2641 ataagtgtat acaaatttta aagtgactct taggttttaa aacgaaaatt cttattcttg
     2701 agtaactctt tcctgtaggt caggttgctt tctcaggtat agcatgaggt cgctcttatt
     2761 gaccacacct ctaccggcat gccaattcac tggccgtcgt tttacaacgt cgtgactggg
     2821 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     2881 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     2941 aatggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcataa
     3001 tcggatcgta cttgttaccc atcattgaat tttgaacatc cgaacctggg agttttccct
     3061 gaaacagata gtatatttga acctgtataa taatatatag tctagcgctt tacggaagac
     3121 aatgtatgta tttcggttcc tggagaaact attgcatcta ttgcataggt aatcttgcac
     3181 gtcgcatccc cggttcattt tctgcgtttc catcttgcac ttcaatagca tatctttgtt
     3241 aacgaagcat ctgtgcttca ttttgtagaa caaaaatgca acgcgagagc gctaattttt
     3301 caaacaaaga atctgagctg catttttaca gaacagaaat gcaacgcgaa agcgctattt
     3361 taccaacgaa gaatctgtgc ttcatttttg taaaacaaaa atgcaacgcg agagcgctaa
     3421 tttttcaaac aaagaatctg agctgcattt ttacagaaca gaaatgcaac gcgagagcgc
     3481 tattttacca acaaagaatc tatacttctt ttttgttcta caaaaatgca tcccgagagc
     3541 gctatttttc taacaaagca tcttagatta ctttttttct cctttgtgcg ctctataatg
     3601 cagtctcttg ataacttttt gcactgtagg tccgttaagg ttagaagaag gctactttgg
     3661 tgtctatttt ctcttccata aaaaaagcct gactccactt cccgcgttta ctgattacta
     3721 gcgaagctgc gggtgcattt tttcaagata aaggcatccc cgattatatt ctataccgat
     3781 gtggattgcg catactttgt gaacagaaag tgatagcgtt gatgattctt cattggtcag
     3841 aaaattatga acggtttctt ctattttgtc tctatatact acgtatagga aatgtttaca
     3901 ttttcgtatt gttttcgatt cactctatga atagttctta ctacaatttt tttgtctaaa
     3961 gagtaatact agagataaac ataaaaaatg tagaggtcga gtttagatgc aagttcaagg
     4021 agcgaaaggt ggatgggtag gttatatagg gatatagcac agagatatat agcaaagaga
     4081 tacttttgag caatgtttgt ggaagcggta ttcgcaatat tttagtagct cgttacagtc
     4141 cggtgcgttt ttggtttttt gaaagtgcgt cttcagagcg cttttggttt tcaaaagcgc
     4201 tctgaagttc ctatactttc tagctagaga ataggaactt cggaatagga acttcaaagc
     4261 gtttccgaaa acgagcgctt ccgaaaatgc aacgcgagct gcgcacatac agctcactgt
     4321 tcacgtcgca cctatatctg cgtgttgcct gtatatatat atacatgaga agaacggcat
     4381 agtgcgtgtt tatgcttaaa tgcgtactta tatgcgtcta tttatgtagg atgaaaggta
     4441 gtctagtacc tcctgtgata ttatcccatt ccatgcgggg tatcgtatgc ttccttcagc
     4501 actacccttt agctgttcta tatgctgcca ctcctcaatt ggattagtct catccttcaa
     4561 tgctatcatt tcctttgata ttggatcgat ccgatgataa gctgtcaaac atgagaattg
     4621 ggtaataact gatataatta aattgaagct ctaatttgtg agtttagtat acatgcattt
     4681 acttataata cagtttttta gttttgctgg ccgcatcttc tcaaatatgc ttcccagcct
     4741 gcttttctgt aacgttcacc ctctacctta gcatcccttc cctttgcaaa tagtcctctt
     4801 ccaacaataa taatgtcaga tcctgtagag accacatcat ccacggttct atactgttga
     4861 cccaatgcgt ctcccttgtc atctaaaccc acaccgggtg tcataatcaa ccaatcgtaa
     4921 ccttcatctc ttccacccat gtctctttga gcaataaagc cgataacaaa atctttgtcg
     4981 ctcttcgcaa tgtcaacagt acccttagta tattctccag tagataggga gcccttgcat
     5041 gacaattctg ctaacatcaa aaggcctcta ggttcctttg ttacttcttc tgccgcctgc
     5101 ttcaaaccgc taacaatacc tgggcccacc acaccgtgtg cattcgtaat gtctgcccat
     5161 tctgctattc tgtatacacc cgcagagtac tgcaatttga ctgtattacc aatgtcagca
     5221 aattttctgt cttcgaagag taaaaaattg tacttggcgg ataatgcctt tagcggctta
     5281 actgtgccct ccatggaaaa atcagtcaag atatccacat gtgtttttag taaacaaatt
     5341 ttgggaccta atgcttcaac taactccagt aattccttgg tggtacgaac atccaatgaa
     5401 gcacacaagt ttgtttgctt ttcgtgcatg atattaaata gcttggcagc aacaggacta
     5461 ggatgagtag cagcacgttc cttatatgta gctttcgaca tgatttatct tcgtttcctg
     5521 catgtttttg ttctgtgcag ttgggttaag aatactgggc aatttcatgt ttcttcaaca
     5581 ctacatatgc gtatatatac caatctaagt ctgtgctcct tccttcgttc ttccttctgt
     5641 tcggagatta ccgaatcaaa aaaatttcaa ggaaaccgaa atcaaaaaaa agaataaaaa
     5701 aaaaatgatg aattgaaaag ctaattcttg aagacgaaag ggcctcgtga tacgcctatt
     5761 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     5821 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     5881 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     5941 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     6001 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     6061 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     6121 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     6181 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     6241 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     6301 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     6361 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     6421 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     6481 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     6541 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     6601 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     6661 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     6721 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     6781 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     6841 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     6901 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     6961 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     7021 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     7081 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     7141 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     7201 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     7261 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     7321 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     7381 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     7441 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     7501 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     7561 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     7621 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     7681 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     7741 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     7801 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     7861 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     7921 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc ttaccgcatc
     7981 aggaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
     8041 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
     8101 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
     8161 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
     8221 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
     8281 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
     8341 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
     8401 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc cattcgc
//
