LOCUS       sequence-437595-        9206 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  equimolar expression of ytd8oxStayGold and mCherry in budding yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9206)
  AUTHORS   Teusink B
  TITLE     Expression of FPs in budding yeast
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9206)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pDRF1 ytd8oxStayGold-T2A-mCherry
FEATURES             Location/Qualifiers
     source          1..9206
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    800..824
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2301..2354
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      2370..2379
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2376..3086
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2521..2539)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2789..2808
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(3096..3120)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(3182..3201)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3183..3201)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      3349..3536
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(3404..3428)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(3548..3565)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3548..3564)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3557..3579)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4000..5346
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(5457..6260)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(6261..6476)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(6501..6519)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6587..6691
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6692..6760,6761..7552)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6910..6929)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7723..8311
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8212..8231
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8465..8482
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8599..8620
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8635..8640,8641..8658,8659..8665)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8673..8689
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8678..8700
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8697..8713
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8697..8713
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(8743..9198)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8880..8901)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9092..9111
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 caagcttcct gaaacggaga aacataaaca ggcattgctg ggatcaccca tacatcactc
       61 tgttttgcct gaccttttcc ggtaatttga aaacaaaccc ggtctcgaag cggagatccg
      121 gcgataatta ccgcagaaat aaacccatac acgagacgta gaaccagccg cacatggccg
      181 gagaaactcc tgcgagaatt tcgtaaactc gcgcgcattg catctgtatt tcctaatgcg
      241 gcacttccag gcctcgatcg agaccgttta tccattgctt ttttgttgtc tttttccctc
      301 gttcacagaa agtctgaaga agctatagta gaactatgag ctttttttgt ttctgttttc
      361 cttttttttt tttttacctc tgtggaaatt gttactctca cactctttag ttcgtttgtt
      421 tgttttgttt attccaatta tgaccggtga cgaaacgtgg tcgatggtgg gtaccgctta
      481 tgctcccctc cattagtttc gattatataa aaaggccaaa tattgtatta ttttcaaatg
      541 tcctatcatt atcgtctaac atctaatttc tcttaaattt tttctctttc tttcctataa
      601 caccaatagt gaaaatcttt ttttcttcta tatctacaaa aacttttttt ttctatcaac
      661 ctcgttgata aattttttct ttaacaatcg ttaataatta attaattgga aaataaccat
      721 tttttctctc ttttatacac acattcaaaa gaaagaaaaa aaatataccc cagcctcgac
      781 tagtgaattc gcggccatca caagtttgta caaaaaagca ggctgctagc cccgggacta
      841 gtaagcttat ggtttctact ggtgaagaat tgtttactgg tgttgttcca tttaagttcc
      901 aattgaaggg taccattaac ggtaagtctt tcactgttga aggtgaaggt gagggtaact
      961 ctcatgaagg ttctcataag ggtaagtatg tttgtacttc cggtaagttg ccaatgtctt
     1021 gggctgctct aggtacttct ttcggttatg gtatgaaata ttacactaaa tacccatctg
     1081 gtttaaagaa ttggttccat gaagtcatgc ctgaaggttt cacttacgat agacatattc
     1141 aatataaagg tgatggttct attcatgcta agcatcaaca tttcatgaag aatggtactt
     1201 atcacaacat tgttgaattc acaggtcaag attttaaaga aaactctcca gttttgaccg
     1261 gtgatatgaa cgtttctttg ccaaacgaag tccaacacat tccaagagac gatggtgttg
     1321 aatgtccagt tactttgttg tatccattgt tgtctgataa atctaagtgt gttgaggctt
     1381 atcaaaacac aattatcaag ccattgcata atcaaccagc ccctgatgtt ccatatcact
     1441 ggatcagaaa gcaatacact caatctaagg atgatactga agaaagagat catattatcc
     1501 aatctgaaac cttagaagct catttgtaca gtagaactaa attagaaggt catggtactg
     1561 gttctactgg ttctggttct tctggtactg cttcctctga agataataat attgatatgg
     1621 ttagcactgg tgaagaattg ttcactggtg ttgttccatt taagttccaa ttgaaaggta
     1681 ccattaacgg taagtcattt acagtcgaag gtgaaggtga agggaactct catgaaggtt
     1741 cacacaaagg taagtacgtc tgtacatccg gtaagttgcc tatgagttgg gctgctttag
     1801 gtacttcttt cggttacggt atgaaatact acactaaata cccatccggt ttgaagaatt
     1861 ggttccatga agttatgcct gaaggtttca cttatgatag acacattcaa tacaagggtg
     1921 atggttcaat tcacgctaag caccaacatt ttatgaaaaa cggtacttac cataacattg
     1981 ttgaattcac cggtcaagat tttaaggaaa attctccagt tttaactggt gatatgaacg
     2041 tctccttgcc aaatgaagtt caacacatcc caagagatga tggtgttgaa tgtccagtca
     2101 ccttgttgta tccattatta tctgataaat ctaagtgtgt tgaagcctac caaaacacta
     2161 taattaagcc attgcacaac caaccagctc ctgatgttcc ataccattgg attagaaagc
     2221 aatacactca aagtaaggat gatactgaag aaagagatca tattattcaa tctgaaactt
     2281 tggaagctca tttgtccgga gagggcagag gaagtcttct aacatgcggt gacgtggagg
     2341 agaatcccgg ccctggggat ccaccggtcg ccaccatggt gagcaagggc gaggaggata
     2401 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg
     2461 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
     2521 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
     2581 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga
     2641 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
     2701 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc
     2761 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg
     2821 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga
     2881 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca
     2941 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc
     3001 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg
     3061 gcggcatgga cgagctgtac aagtaaagcg gccgcaccca gctttcttgt acaaagtggt
     3121 gtagttaatt catgatggcc gctgcaggtc gacctcgagg gggggcccgg tacccaattc
     3181 gccctatagt gagtcgtatt acgcgcggat ccagctttgg acttcttcgc cagaggtttg
     3241 gtcaagtctc caatcaaggt tgtcggcttg tctaccttgc cagaaattta cgaaaagatg
     3301 gaaaagggtc aaatcgttgg tagatacgtt gttgacactt ctaaataagc gaatttctta
     3361 tgatttatga tttttattat taaataagtt ataaaaaaaa taagtgtata caaattttaa
     3421 agtgactctt aggttttaaa acgaaaattc ttattcttga gtaactcttt cctgtaggtc
     3481 aggttgcttt ctcaggtata gcatgaggtc gctcttattg accacacctc taccggcatg
     3541 ccaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     3601 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     3661 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt
     3721 ttctccttac gcatctgtgc ggtatttcac accgcataat cggatcgtac ttgttaccca
     3781 tcattgaatt ttgaacatcc gaacctggaa gttttccctg aaacagatag tatatttgaa
     3841 cctgtataat aatatatagt ctagcgcttt acggaagaca atgtatgtat ttcggttcct
     3901 ggagaaacta ttgcatctat tgcataggta atcttgcacg tcgcatcccc ggttcatttt
     3961 ctgcgtttcc atcttgcact tcaatagcat atctttgtta acgaagcatc tgtgcttcat
     4021 tttgtagaac aaaaatgcaa cgcgagagcg ctaatttttc aaacaaagaa tctgagctgc
     4081 atttttacag aacagaaatg caacgcgaaa gcgctatttt accaacgaag aatctgtgct
     4141 tcatttttgt aaaacaaaaa tgcaacgcga gagcgctaat ttttcaaaca aagaatctga
     4201 gctgcatttt tacagaacag aaatgcaacg cgagagcgct attttaccaa caaagaatct
     4261 atacttcttt tttgttctac aaaaatgcat cccgagagcg ctatttttct aacaaagcat
     4321 cttagattac tttttttctc ctttgtgcgc tctataatgc agtctcttga taactttttg
     4381 cactgtaggt ccgttaaggt tagaagaagg ctactttggt gtctattttc tcttccataa
     4441 aaaaagcctg actccacttc ccgcgtttac tgattactag cgaagctgcg ggtgcatttt
     4501 ttcaagataa aggcatcccc gattatattc tataccgatg tggattgcgc atactttgtg
     4561 aacagaaagt gatagcgttg atgattcttc attggtcaga aaattatgaa cggtttcttc
     4621 tattttgtct ctatatacta cgtataggaa atgtttacat tttcgtattg ttttcgattc
     4681 actctatgaa tagttcttac tacaattttt ttgtctaaag agtaatacta gagataaaca
     4741 taaaaaatgt agaggtcgag tttagatgca agttcaagga gcgaaaggtg gatgggtagg
     4801 ttatataggg atatagcaca gagatatata gcaaagagat acttttgagc aatgtttgtg
     4861 gaagcggtat tcgcaatatt ttagtagctc gttacagtcc ggtgcgtttt tggttttttg
     4921 aaagtgcgtc ttcagagcgc ttttggtttt caaaagcgct ctgaagttcc tatactttct
     4981 agctagagaa taggaacttc ggaataggaa cttcaaagcg tttccgaaaa cgagcgcttc
     5041 cgaaaatgca acgcgagctg cgcacataca gctcactgtt cacgtcgcac ctatatctgc
     5101 gtgttgcctg tatatatata tacatgagaa gaacggcata gtgcgtgttt atgcttaaat
     5161 gcgtacttat atgcgtctat ttatgtagga tgaaaggtag tctagtacct cctgtgatat
     5221 tatcccattc catgcggggt atcgtatgct tccttcagca ctacccttta gctgttctat
     5281 atgctgccac tcctcaattg gattagtctc atccttcaat gctatcattt cctttgatat
     5341 tggatcgatc cgatgataag ctgtcaaaca tgagaattgg gtaataactg atataattaa
     5401 attgaagctc taatttgtga gtttagtata catgcattta cttataatac agttttttag
     5461 ttttgctggc cgcatcttct caaatatgct tcccagcctg cttttctgta acgttcaccc
     5521 tctaccttag catcccttcc ctttgcaaat agtcctcttc caacaataat aatgtcagat
     5581 cctgtagaga ccacatcatc cacggttcta tactgttgac ccaatgcgtc tcccttgtca
     5641 tctaaaccca caccgggtgt cataatcaac caatcgtaac cttcatctct tccacccatg
     5701 tctctttgag caataaagcc gataacaaaa tctttgtcgc tcttcgcaat gtcaacagta
     5761 cccttagtat attctccagt agatagggag cccttgcatg acaattctgc taacatcaaa
     5821 aggcctctag gttcctttgt tacttcttct gccgcctgct tcaaaccgct aacaatacct
     5881 gggcccacca caccgtgtgc attcgtaatg tctgcccatt ctgctattct gtatacaccc
     5941 gcagagtact gcaatttgac tgtattacca atgtcagcaa attttctgtc ttcgaagagt
     6001 aaaaaattgt acttggcgga taatgccttt agcggcttaa ctgtgccctc catggaaaaa
     6061 tcagtcaaga tatccacatg tgtttttagt aaacaaattt tgggacctaa tgcttcaact
     6121 aactccagta attccttggt ggtacgaaca tccaatgaag cacacaagtt tgtttgcttt
     6181 tcgtgcatga tattaaatag cttggcagca acaggactag gatgagtagc agcacgttcc
     6241 ttatatgtag ctttcgacat gatttatctt cgtttcctgc atgtttttgt tctgtgcagt
     6301 tgggttaaga atactgggca atttcatgtt tcttcaacac tacatatgcg tatatatacc
     6361 aatctaagtc tgtgctcctt ccttcgttct tccttctgtt cggagattac cgaatcaaaa
     6421 aaatttcaag gaaaccgaaa tcaaaaaaaa gaataaaaaa aaaatgatga attgaaaagc
     6481 taattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     6541 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     6601 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     6661 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     6721 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     6781 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     6841 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     6901 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     6961 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     7021 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     7081 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     7141 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     7201 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     7261 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     7321 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     7381 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     7441 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     7501 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     7561 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     7621 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     7681 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     7741 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     7801 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     7861 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     7921 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     7981 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     8041 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     8101 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     8161 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     8221 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     8281 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     8341 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     8401 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     8461 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     8521 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     8581 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     8641 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     8701 aaacagctat gaccatgatt acgccaagct taccgcatca ggaaattgta agcgttaata
     8761 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
     8821 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
     8881 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
     8941 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
     9001 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
     9061 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
     9121 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
     9181 cgccgctaca gggcgcgtcc attcgc
//
