LOCUS       sequence-437596-        4740 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  tagging vector to tag genes with ymScarletI and a HIS5 gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4740)
  AUTHORS   Teusink B
  TITLE     Expression of FPs in budding yeast
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pFA6a-link-ymScarletI-SpHis5
FEATURES             Location/Qualifiers
     source          1..4740
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     327..346
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(565..669)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     737..755
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(793..815)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     915..934
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        1015..1033
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1015..1032
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     terminator      1842..2029
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1897..1921)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     gene            2104..3304
                     /label=HIS3MX6
                     /note="yeast selectable marker encoding the S. pombe his5 
                     gene, which corresponds to S. cerevisiae HIS3"
     promoter        2104..2447
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             2448..3101
                     /codon_start=1
                     /gene="Schizosaccharomyces pombe his5"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=S. pombe his5
                     /note="yeast auxotrophic marker; corresponds to S. 
                     cerevisiae HIS3"
                     /translation="MGRRAFVERNTNETKISVAIALDKAPLPEESNFIDELITSKHTNQ
                     KGEQVIQVDTGIGFLDHMYHALAKHAGWSLRLYSRGDLIIDDHHTAEDTAIALGIAFKQ
                     AMSNFAGVKRFGHAYCPLDEALSRSVVDLSGRPYAVIDLGLKREKVGELSCEMIPHLLY
                     SFSVAAGITLHVTCLYGSNDHHRAESAFKSLAVAMRAATSLTGSSEVPSTKGVL"
     terminator      3107..3304
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(3408..3427)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3409..3427)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3514..3531)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3685..4273)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3765..3784)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4444..4740,1..495,496..564))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
       61 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
      121 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
      181 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
      241 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
      301 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
      361 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
      421 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
      481 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
      541 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
      601 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
      661 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
      721 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga
      781 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
      841 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
      901 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tggacatatt
      961 gtcgttagaa cgcggctaca attaatacat aaccttatgt atcatacaca tacgatttag
     1021 gtgacactat agaacgcggc cgccagctga agcttcgtac gctgcaggtc gacggatcgg
     1081 tgacggtgct ggtttaatta acatggttag taaaggtgaa gctgttataa aagaattcat
     1141 gaggtttaaa gttcatatgg aaggttcaat gaatggtcat gaatttgaaa ttgaaggtga
     1201 aggtgaaggt agaccatatg aaggtacaca aactgctaaa ttgaaagtta ctaaaggtgg
     1261 tccattacca ttttcttggg atattttgtc tccacaattc atgtatggtt ctagagcttt
     1321 tattaagcat ccagctgata ttccagatta ttataaacaa tcttttcctg aaggttttaa
     1381 atgggaaaga gttatgaatt tcgaagatgg tggtgctgtt actgttactc aagatacttc
     1441 tttggaagat ggtactttaa tctataaagt taaattgaga ggtactaatt ttccaccaga
     1501 tggtccagtt atgcaaaaga aaactatggg ttgggaagca tctactgaaa gattgtatcc
     1561 agaagatggt gttttgaaag gagacattaa gatggctttg agattgaaag atggtggtcg
     1621 gtacctggcc gacttcaaga ccacctataa agctaaaaaa ccagttcaaa tgccaggtgc
     1681 atataatgtt gatagaaagt tagatataac gtcgcataac gaggactata ctgtagttga
     1741 acaatatgaa cgtagtgaag gtagacatag taccggagga atggatgaat tgtataaata
     1801 agcggccgcc tcgaggtcga cggcgcgcca cttctaaata agcgaatttc ttatgattta
     1861 tgatttttat tattaaataa gttataaaaa aaataagtgt atacaaattt taaagtgact
     1921 cttaggtttt aaaacgaaaa ttcttattct tgagtaactc tttcctgtag gtcaggttgc
     1981 tttctcaggt atagtatgag gtcgctctta ttgaccacac ctctaccggc agatccgcta
     2041 gggataacag ggtaatatag atctgtttag cttgcctcgt ccccgccggg tcacccggcc
     2101 agcgacatgg aggcccagaa taccctcctt gacagtcttg acgtgcgcag ctcaggggca
     2161 tgatgtgact gtcgcccgta catttagccc atacatcccc atgtataatc atttgcatcc
     2221 atacattttg atggccgcac ggcgcgaagc aaaaattacg gctcctcgct gcagacctgc
     2281 gagcagggaa acgctcccct cacagacgcg ttgaattgtc cccacgccgc gcccctgtag
     2341 agaaatataa aaggttagga tttgccactg aggttcttct ttcatatact tccttttaaa
     2401 atcttgctag gatacagttc tcacatcaca tccgaacata aacaaccatg ggtaggaggg
     2461 cttttgtaga aagaaatacg aacgaaacga aaatcagcgt tgccatcgct ttggacaaag
     2521 ctcccttacc tgaagagtcg aattttattg atgaacttat aacttccaag catacaaacc
     2581 aaaagggaga acaagtaatc caagtagaca cgggaattgg attcttggat cacatgtatc
     2641 atgcactggc taaacatgca ggctggagct tacgacttta ctcaagaggt gatttaatca
     2701 tcgatgatca tcacactgca gaagatactg ctattgcact tggtattgca ttcaagcagg
     2761 ctatgagtaa ctttgccggc gttaaaagat ttggacatgc ttattgtcca cttgacgaag
     2821 ctctttctag aagcgtagtt gacttgtcgg gacggcccta tgctgttatc gatttgggat
     2881 taaagcgtga aaaggttggg gaattgtcct gtgaaatgat ccctcactta ctatattcct
     2941 tttcggtagc agctggaatt actttgcatg ttacctgctt atatggtagt aatgaccatc
     3001 atcgtgctga aagcgctttt aaatctctgg ctgttgccat gcgcgcggct actagtctta
     3061 ctggaagttc tgaagtccca agcacgaagg gagtgttgta aagagtactg acaataaaaa
     3121 gattcttgtt ttcaagaact tgtcatttgt atagtttttt tatattgtag ttgttctatt
     3181 ttaatcaaat gttagcgtga tttatatttt ttttcgcctc gacatcatct gcccagatgc
     3241 gaagttaagt gcgcagaaag taatatcatg cgtcaatcgt atgtgaatgc tggtcgctat
     3301 actgctgtcg attcgatact aacgccgcca tccagtttaa acgagctcga attcatcgat
     3361 gatatcagat ccactagtgg cctatgcggc cgcggatctg ccggtctccc tatagtgagt
     3421 cgtattaatt tcgataagcc aggttaacct gcattaatga atcggccaac gcgcggggag
     3481 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     3541 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     3601 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     3661 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     3721 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     3781 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     3841 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     3901 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     3961 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     4021 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     4081 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     4141 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     4201 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     4261 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     4321 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     4381 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     4441 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     4501 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     4561 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     4621 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     4681 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
//
