LOCUS       sequence-437701-       12033 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of CBP IDR6 fragment fused to optogenetic protein 
            mCh-Cry2WT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12033)
  TITLE     Bose Lab CBP Expression Plasmids
REFERENCE   2  (bases 1 to 12033)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHR-IDR6-mCh-Cry2WT-NLS
FEATURES             Location/Qualifiers
     source          1..12033
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    43..160
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        313..720
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             2215..3708
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     CDS             3805..3825
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    3890..4478
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3943..3963)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4361..4372)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4481..4497)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4482..4498)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4588..4821
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4846..4864)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4847..4864)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5154..5173)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5387..5406)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5506..5528
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5566..5584)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5652..5756
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5757..5825,5826..6617)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5975..5994)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6788..7376
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7277..7296
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7530..7547
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7617..7637)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7622..7951
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7802..7937
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9085..9150
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9280..9300
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9725..9859
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9762..9781)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9816..9835
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10027..10660
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10707..10832
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11329..11562
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11747..11791
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cggatctcga cggtatcgcc aaatggcagt attcatccac aattttaaaa gaaaaggggg
       61 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
      121 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
      181 cagagatcca gtttggatcg ataagcttga tatcgaattc ctgcagcccc gataaaataa
      241 aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc
      301 aagctagctg cagtaacgcc attttgcaag gcatggaaaa ataccaaacc aagaatagag
      361 aagttcagat caagggcggg tacatgaaaa tagctaacgt tgggccaaac aggatatctg
      421 cggtgagcag tttcggcccc ggcccggggc caagaacaga tggtcaccgc agtttcggcc
      481 ccggcccgag gccaagaaca gatggtcccc agatatggcc caaccctcag cagtttctta
      541 agacccatca gatgtttcca ggctccccca aggacctgaa atgaccctgc gccttatttg
      601 aattaaccaa tcagcctgct tctcgcttct gttcgcgcgc ttctgcttcc cgagctctat
      661 aaaagagctc acaacccctc actcggcgcg ccagtcctcc gacagactga gtcgcccggg
      721 ggggatctgg agctctcgag aattctcacg cagcaccgcc tgcagcaggc ccagctcatg
      781 cgccggcgga tggccaccat gaacacccgc aacgtgcctc agcagagtct gccttctcct
      841 acctcagcac cgcccgggac ccccacacag cagcccagca caccccagac gccgcagccc
      901 cctgcccagc cccaaccctc acccgtgagc atgtcaccag ctggcttccc cagcgtggcc
      961 cggactcagc cccccaccac ggtgtccaca gggaagccta ccagccaggt gccggccccc
     1021 ccacccccgg cccagccccc tcctgcagcg gtggaagcgg ctcggcagat cgagcgtgag
     1081 gcccagcagc agcagcacct gtaccgggtg aacatcaaca acagcatgcc cccaggacgc
     1141 acgggcatgg ggaccccggg gagccagatg gcccccgtga gcctgaatgt gccccgaccc
     1201 aaccaggtga gcgggcccgt catgcccagc atgcctcccg ggcagtggca gcaggcgccc
     1261 cttccccagc agcagcccat gccaggcttg cccaggcctg tgatatccat gcaggcccag
     1321 gcggccgtgg ctgggccccg gatgcccagc gtgcagccac ccaggagcat ctcacccagc
     1381 gctctgcaag acctgctgcg gaccctgaag tcgcccagct cccctcagca gcaacagcag
     1441 gtgctgaaca ttctcagtgg agcaaggcag gtggacagtg gatccggagc taccatggtg
     1501 tctaaaggcg aggaggataa catggctata atcaaggaat ttatgaggtt caaggtgcat
     1561 atggagggat ctgtgaacgg tcacgagttc gaaatcgagg gcgaaggcga ggggcgaccc
     1621 tatgaaggga cacagacggc caaactgaag gtgaccaagg gtggccccct gcctttcgcc
     1681 tgggacatcc tgtcacccca gtttatgtac ggctctaagg cttacgttaa gcaccctgcc
     1741 gatattccgg actacttgaa actgagcttc cctgaagggt tcaagtggga acgggtaatg
     1801 aattttgagg atggcggtgt agtcacagtt acccaggaca gctctcttca ggacggagaa
     1861 tttatctata aggttaaact tcggggcact aacttcccat cagacggccc cgtgatgcag
     1921 aagaaaacta tgggctggga agccagctca gaacgcatgt atcccgagga cggagcactg
     1981 aagggagaaa tcaagcagcg actcaagctg aaggatgggg gacattatga tgctgaggtc
     2041 aaaaccacct ataaggccaa gaaacccgtt cagcttcctg gggcatacaa cgtgaatatc
     2101 aaactggata ttacatccca taacgaagac tataccatcg tggaacagta cgagcgggcc
     2161 gagggcagac atagcacagg gggcatggat gaattgtaca aggcggccac gcgtatgaag
     2221 atggacaaaa agactatagt ttggtttaga agagacctaa ggattgagga taatcctgca
     2281 ttagcagcag ctgctcacga aggatctgtt tttcctgtct tcatttggtg tcctgaagaa
     2341 gaaggacagt tttatcctgg aagagcttca agatggtgga tgaaacaatc acttgctcac
     2401 ttatctcaat ccttgaaggc tcttggatct gacctcactt taatcaaaac ccacaacacg
     2461 atttcagcga tcttggattg tatccgcgtt accggtgcta caaaagtcgt ctttaaccac
     2521 ctctatgatc ctgtttcgtt agttcgggac cataccgtaa aggagaagct ggtggaacgt
     2581 gggatctctg tgcaaagcta caatggagat ctattgtatg aaccgtggga gatatactgc
     2641 gaaaagggca aaccttttac gagtttcaat tcttactgga agaaatgctt agatatgtcg
     2701 attgaatccg ttatgcttcc tcctccttgg cggttgatgc caataactgc agcggctgaa
     2761 gcgatttggg cgtgttcgat tgaagaacta gggctggaga atgaggccga gaaaccgagc
     2821 aatgcgttgt taactagagc ttggtctcca ggatggagca atgctgataa gttactaaat
     2881 gagttcatcg agaagcagtt gatagattat gcaaagaaca gcaagaaagt tgttgggaat
     2941 tctacttcac tactttctcc gtatctccat ttcggggaaa taagcgtcag acacgttttc
     3001 cagtgtgccc ggatgaaaca aattatatgg gcaagagata agaacagtga aggagaagaa
     3061 agtgcagatc tttttcttag gggaatcggt ttaagagagt attctcggta tatatgtttc
     3121 aacttcccgt ttactcacga gcaatcgttg ttgagtcatc ttcggttttt cccttgggat
     3181 gctgatgttg ataagttcaa ggcctggaga caaggcagga ccggttatcc gttggtggat
     3241 gccggaatga gagagctttg ggctaccgga tggatgcata acagaataag agtgattgtt
     3301 tcaagctttg ctgtgaagtt tcttctcctt ccatggaaat ggggaatgaa gtatttctgg
     3361 gatacacttt tggatgctga tttggaatgt gacatccttg gctggcagta tatctctggg
     3421 agtatccccg atggccacga gcttgatcgc ttggacaatc ccgcgttaca aggcgccaaa
     3481 tatgacccag aaggtgagta cataaggcaa tggcttcccg agcttgcgag attgccaact
     3541 gaatggatcc atcatccatg ggacgctcct ttaaccgtac tcaaagcttc tggtgtggaa
     3601 ctcggaacaa actatgcgaa acccattgta gacatcgaca cagctcgtga gctactagct
     3661 aaagctattt caagaacccg tgaagcacag atcatgatcg gagcagcagc ccgggatcca
     3721 ccggtcgcca ccggttcagg gagcggatcc ggctcaggga gtgcggccgc aactcccacc
     3781 tgcaacatgc gtgacggagg cggtccaaaa aagaagagaa aggtatgact gaggccgcga
     3841 ctctagagtc gacctgcagg catgcaagct tgatatcaag cttatcgata atcaacctct
     3901 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     3961 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     4021 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     4081 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     4141 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     4201 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     4261 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc
     4321 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     4381 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     4441 tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc gacctcgagg
     4501 gaattaattc gagctcggta cctttaagac caatgactta caaggcagct gtagatctta
     4561 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     4621 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     4681 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     4741 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     4801 cagtgtggaa aatctctagc agcatctaga attaattccg tgtattctat agtgtcacct
     4861 aaatcgtatg tgtatgatac ataaggttat gtattaattg tagccgcgtt ctaacgacaa
     4921 tatgtacaag cctaattgtg tagcatctgg cttactgaag cagaccctat catctctctc
     4981 gtaaactgcc gtcagagtcg gtttggttgg acgaaccttc tgagtttctg gtaacgccgt
     5041 cccgcacccg gaaatggtca gcgaaccaat cagcagggtc atcgctagcc agatcctcta
     5101 cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat
     5161 cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt
     5221 cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca
     5281 tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct
     5341 aatgcaggag tcgcataagg gagagcgtcg aatggtgcac tctcagtaca atctgctctg
     5401 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     5461 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     5521 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
     5581 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     5641 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     5701 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     5761 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     5821 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     5881 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     5941 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     6001 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     6061 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     6121 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     6181 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     6241 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     6301 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     6361 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     6421 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     6481 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     6541 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     6601 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     6661 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     6721 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     6781 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     6841 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     6901 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     6961 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     7021 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     7081 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     7141 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     7201 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     7261 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     7321 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     7381 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     7441 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     7501 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     7561 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctgtggaa
     7621 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     7681 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     7741 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     7801 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     7861 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     7921 aggctttttt ggaggcctag gcttttgcaa aaagcttgga cacaagacag gcttgcgaga
     7981 tatgtttgag aataccactt tatcccgcgt cagggagagg cagtgcgtaa aaagacgcgg
     8041 actcatgtga aatactggtt tttagtgcgc cagatctcta taatctcgcg caacctattt
     8101 tcccctcgaa cactttttaa gccgtagata aacaggctgg gacacttcac atgagcgaaa
     8161 aatacatcgt cacctgggac atgttgcaga tccatgcacg taaactcgca agccgactga
     8221 tgccttctga acaatggaaa ggcattattg ccgtaagccg tggcggtctg taccgggtgc
     8281 gttactggcg cgtgaactgg gtattcgtca tgtcgatacc gtttgtattt ccagctacga
     8341 tcacgacaac cagcgcgagc ttaaagtgct gaaacgcgca gaaggcgatg gcgaaggctt
     8401 catcgttatt gatgacctgg tggataccgg tggtactgcg gttgcgattc gtgaaatgta
     8461 tccaaaagcg cactttgtca ccatcttcgc aaaaccggct ggtcgtccgc tggttgatga
     8521 ctatgttgtt gatatcccgc aagatacctg gattgaacag ccgtgggata tgggcgtcgt
     8581 attcgtcccg ccaatctccg gtcgctaatc ttttcaacgc ctggcactgc cgggcgttgt
     8641 tctttttaac ttcaggcggg ttacaatagt ttccagtaag tattctggag gctgcatcca
     8701 tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg ctttaaacat cctgaaacct
     8761 cgacgctagt ccgccgcttt aatcacggcg cacaaccgcc tgtgcagtcg gcccttgatg
     8821 gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct gatgtggatc tggcgcggca
     8881 ttgacccacg cgaaatcctc gacgtccagg cacgtattgt gatgagcgat gccgaacgta
     8941 ccgacgatga tttatacgat acggtgattg gctaccgtgg cggcaactgg atttatgagt
     9001 gggccccgga tctttgtgaa ggaaccttac ttctgtggtg tgacataatt ggacaaacta
     9061 cctacagaga tttaaagctc taaggtaaat ataaaatttt taagtgtata atgtgttaaa
     9121 ctactgattc taattgtttg tgtattttag attccaacct atggaactga tgaatgggag
     9181 cagtggtgga atgcctttaa tgaggaaaac ctgttttgct cagaagaaat gccatctagt
     9241 gatgatgagg ctactgctga ctctcaacat tctactcctc caaaaaagaa gagaaaggta
     9301 gaagacccca aggactttcc ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt
     9361 aatagaactc ttgcttgctt tgctatttac accacaaagg aaaaagctgc actgctatac
     9421 aagaaaatta tggaaaaata ttctgtaacc tttataagta ggcataacag ttataatcat
     9481 aacatactgt tttttcttac tccacacagg catagagtgt ctgctattaa taactatgct
     9541 caaaaattgt gtacctttag ctttttaatt tgtaaagggg ttaataagga atatttgatg
     9601 tatagtgcct tgactagaga tcataatcag ccataccaca tttgtagagg ttttacttgc
     9661 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
     9721 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     9781 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     9841 atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc
     9901 caccccatac cctattacca ctgccaatta cctgtggttt catttactct aaacctgtga
     9961 ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt
    10021 agtagttgga agggctaatt cactcccaaa gaagacaaga tatccttgat ctgtggatct
    10081 accacacaca aggctacttc cctgattagc agaactacac accagggcca ggggtcagat
    10141 atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag
    10201 aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg
    10261 acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg
    10321 cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac
    10381 tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc
    10441 tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag
    10501 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
    10561 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
    10621 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact
    10681 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc
    10741 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag
    10801 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc
    10861 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag
    10921 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag
    10981 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
    11041 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa
    11101 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg
    11161 cacagcaagc ggccggccgc tgatcttcag acctggagga ggagatatga gggacaattg
    11221 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac
    11281 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt
    11341 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt
    11401 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat
    11461 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag
    11521 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc
    11581 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct
    11641 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
    11701 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
    11761 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
    11821 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
    11881 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
    11941 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
    12001 gagagacaga gacagatcca ttcgattagt gaa
//
