LOCUS       sequence-437702-       11640 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of CBP IDR1 fragment fused to optogenetic protein 
            mCh-Cry2WT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11640)
  TITLE     Bose Lab CBP Expression Plasmids
REFERENCE   2  (bases 1 to 11640)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHR-IDR1-mCh-Cry2WT-NLS
FEATURES             Location/Qualifiers
     source          1..11640
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    43..160
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        313..720
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             1822..3315
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     CDS             3412..3432
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    3497..4085
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3550..3570)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3968..3979)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4088..4104)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4089..4105)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4195..4428
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4453..4471)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4454..4471)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4761..4780)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(4994..5013)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5113..5135
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5173..5191)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5259..5363
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5364..5432,5433..6224)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5582..5601)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6395..6983
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6884..6903
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7137..7154
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7224..7244)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7229..7558
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7409..7544
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8692..8757
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8887..8907
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9332..9466
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9369..9388)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9423..9442
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9634..10267
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10314..10439
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10936..11169
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11354..11398
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cggatctcga cggtatcgcc aaatggcagt attcatccac aattttaaaa gaaaaggggg
       61 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
      121 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
      181 cagagatcca gtttggatcg ataagcttga tatcgaattc ctgcagcccc gataaaataa
      241 aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc
      301 aagctagctg cagtaacgcc attttgcaag gcatggaaaa ataccaaacc aagaatagag
      361 aagttcagat caagggcggg tacatgaaaa tagctaacgt tgggccaaac aggatatctg
      421 cggtgagcag tttcggcccc ggcccggggc caagaacaga tggtcaccgc agtttcggcc
      481 ccggcccgag gccaagaaca gatggtcccc agatatggcc caaccctcag cagtttctta
      541 agacccatca gatgtttcca ggctccccca aggacctgaa atgaccctgc gccttatttg
      601 aattaaccaa tcagcctgct tctcgcttct gttcgcgcgc ttctgcttcc cgagctctat
      661 aaaagagctc acaacccctc actcggcgcg ccagtcctcc gacagactga gtcgcccggg
      721 ggggatctgg agctctcgag aattctcacg gagcttctac gaggaggcag cggctctagt
      781 atcaacccag gaataggaaa tgtgagcgcc agcagccccg tgcagcaggg cctgggtggc
      841 caggctcaag ggcagccgaa cagtgctaac atggccagcc tcagtgccat gggcaagagc
      901 cctctgagcc agggagattc ttcagccccc agcctgccta aacaggcagc cagcacctct
      961 gggcccaccc ccgctgcctc ccaagcactg aatccgcaag cacaaaagca agtggggctg
     1021 gcgactagca gccctgccac gtcacagact ggacctggta tcagtggagc aaggcaggtg
     1081 gacagtggat ccggagctac catggtgtct aaaggcgagg aggataacat ggctataatc
     1141 aaggaattta tgaggttcaa ggtgcatatg gagggatctg tgaacggtca cgagttcgaa
     1201 atcgagggcg aaggcgaggg gcgaccctat gaagggacac agacggccaa actgaaggtg
     1261 accaagggtg gccccctgcc tttcgcctgg gacatcctgt caccccagtt tatgtacggc
     1321 tctaaggctt acgttaagca ccctgccgat attccggact acttgaaact gagcttccct
     1381 gaagggttca agtgggaacg ggtaatgaat tttgaggatg gcggtgtagt cacagttacc
     1441 caggacagct ctcttcagga cggagaattt atctataagg ttaaacttcg gggcactaac
     1501 ttcccatcag acggccccgt gatgcagaag aaaactatgg gctgggaagc cagctcagaa
     1561 cgcatgtatc ccgaggacgg agcactgaag ggagaaatca agcagcgact caagctgaag
     1621 gatgggggac attatgatgc tgaggtcaaa accacctata aggccaagaa acccgttcag
     1681 cttcctgggg catacaacgt gaatatcaaa ctggatatta catcccataa cgaagactat
     1741 accatcgtgg aacagtacga gcgggccgag ggcagacata gcacaggggg catggatgaa
     1801 ttgtacaagg cggccacgcg tatgaagatg gacaaaaaga ctatagtttg gtttagaaga
     1861 gacctaagga ttgaggataa tcctgcatta gcagcagctg ctcacgaagg atctgttttt
     1921 cctgtcttca tttggtgtcc tgaagaagaa ggacagtttt atcctggaag agcttcaaga
     1981 tggtggatga aacaatcact tgctcactta tctcaatcct tgaaggctct tggatctgac
     2041 ctcactttaa tcaaaaccca caacacgatt tcagcgatct tggattgtat ccgcgttacc
     2101 ggtgctacaa aagtcgtctt taaccacctc tatgatcctg tttcgttagt tcgggaccat
     2161 accgtaaagg agaagctggt ggaacgtggg atctctgtgc aaagctacaa tggagatcta
     2221 ttgtatgaac cgtgggagat atactgcgaa aagggcaaac cttttacgag tttcaattct
     2281 tactggaaga aatgcttaga tatgtcgatt gaatccgtta tgcttcctcc tccttggcgg
     2341 ttgatgccaa taactgcagc ggctgaagcg atttgggcgt gttcgattga agaactaggg
     2401 ctggagaatg aggccgagaa accgagcaat gcgttgttaa ctagagcttg gtctccagga
     2461 tggagcaatg ctgataagtt actaaatgag ttcatcgaga agcagttgat agattatgca
     2521 aagaacagca agaaagttgt tgggaattct acttcactac tttctccgta tctccatttc
     2581 ggggaaataa gcgtcagaca cgttttccag tgtgcccgga tgaaacaaat tatatgggca
     2641 agagataaga acagtgaagg agaagaaagt gcagatcttt ttcttagggg aatcggttta
     2701 agagagtatt ctcggtatat atgtttcaac ttcccgttta ctcacgagca atcgttgttg
     2761 agtcatcttc ggtttttccc ttgggatgct gatgttgata agttcaaggc ctggagacaa
     2821 ggcaggaccg gttatccgtt ggtggatgcc ggaatgagag agctttgggc taccggatgg
     2881 atgcataaca gaataagagt gattgtttca agctttgctg tgaagtttct tctccttcca
     2941 tggaaatggg gaatgaagta tttctgggat acacttttgg atgctgattt ggaatgtgac
     3001 atccttggct ggcagtatat ctctgggagt atccccgatg gccacgagct tgatcgcttg
     3061 gacaatcccg cgttacaagg cgccaaatat gacccagaag gtgagtacat aaggcaatgg
     3121 cttcccgagc ttgcgagatt gccaactgaa tggatccatc atccatggga cgctccttta
     3181 accgtactca aagcttctgg tgtggaactc ggaacaaact atgcgaaacc cattgtagac
     3241 atcgacacag ctcgtgagct actagctaaa gctatttcaa gaacccgtga agcacagatc
     3301 atgatcggag cagcagcccg ggatccaccg gtcgccaccg gttcagggag cggatccggc
     3361 tcagggagtg cggccgcaac tcccacctgc aacatgcgtg acggaggcgg tccaaaaaag
     3421 aagagaaagg tatgactgag gccgcgactc tagagtcgac ctgcaggcat gcaagcttga
     3481 tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     3541 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     3601 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     3661 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     3721 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     3781 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     3841 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     3901 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     3961 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     4021 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     4081 cccgcatcga taccgtcgac ctcgagggaa ttaattcgag ctcggtacct ttaagaccaa
     4141 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     4201 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     4261 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     4321 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     4381 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagc atctagaatt
     4441 aattccgtgt attctatagt gtcacctaaa tcgtatgtgt atgatacata aggttatgta
     4501 ttaattgtag ccgcgttcta acgacaatat gtacaagcct aattgtgtag catctggctt
     4561 actgaagcag accctatcat ctctctcgta aactgccgtc agagtcggtt tggttggacg
     4621 aaccttctga gtttctggta acgccgtccc gcacccggaa atggtcagcg aaccaatcag
     4681 cagggtcatc gctagccaga tcctctacgc cggacgcatc gtggccggca tcaccggcgc
     4741 cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg
     4801 ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg
     4861 gggactgttg ggcgccatct ccttgcatgc accattcctt gcggcggcgg tgctcaacgg
     4921 cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgaat
     4981 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc
     5041 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     5101 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     5161 cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     5221 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     5281 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     5341 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     5401 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     5461 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     5521 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     5581 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     5641 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     5701 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     5761 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     5821 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     5881 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     5941 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     6001 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     6061 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     6121 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     6181 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     6241 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     6301 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     6361 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     6421 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     6481 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     6541 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     6601 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     6661 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     6721 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     6781 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     6841 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     6901 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     6961 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     7021 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     7081 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     7141 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     7201 ggccgattca ttaatgcagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     7261 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     7321 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     7381 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     7441 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     7501 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     7561 gcttggacac aagacaggct tgcgagatat gtttgagaat accactttat cccgcgtcag
     7621 ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat actggttttt agtgcgccag
     7681 atctctataa tctcgcgcaa cctattttcc cctcgaacac tttttaagcc gtagataaac
     7741 aggctgggac acttcacatg agcgaaaaat acatcgtcac ctgggacatg ttgcagatcc
     7801 atgcacgtaa actcgcaagc cgactgatgc cttctgaaca atggaaaggc attattgccg
     7861 taagccgtgg cggtctgtac cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt
     7921 cgataccgtt tgtatttcca gctacgatca cgacaaccag cgcgagctta aagtgctgaa
     7981 acgcgcagaa ggcgatggcg aaggcttcat cgttattgat gacctggtgg ataccggtgg
     8041 tactgcggtt gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa
     8101 accggctggt cgtccgctgg ttgatgacta tgttgttgat atcccgcaag atacctggat
     8161 tgaacagccg tgggatatgg gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt
     8221 tcaacgcctg gcactgccgg gcgttgttct ttttaacttc aggcgggtta caatagtttc
     8281 cagtaagtat tctggaggct gcatccatga cacaggcaaa cctgagcgaa accctgttca
     8341 aaccccgctt taaacatcct gaaacctcga cgctagtccg ccgctttaat cacggcgcac
     8401 aaccgcctgt gcagtcggcc cttgatggta aaaccatccc tcactggtat cgcatgatta
     8461 accgtctgat gtggatctgg cgcggcattg acccacgcga aatcctcgac gtccaggcac
     8521 gtattgtgat gagcgatgcc gaacgtaccg acgatgattt atacgatacg gtgattggct
     8581 accgtggcgg caactggatt tatgagtggg ccccggatct ttgtgaagga accttacttc
     8641 tgtggtgtga cataattgga caaactacct acagagattt aaagctctaa ggtaaatata
     8701 aaatttttaa gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt
     8761 ccaacctatg gaactgatga atgggagcag tggtggaatg cctttaatga ggaaaacctg
     8821 ttttgctcag aagaaatgcc atctagtgat gatgaggcta ctgctgactc tcaacattct
     8881 actcctccaa aaaagaagag aaaggtagaa gaccccaagg actttccttc agaattgcta
     8941 agttttttga gtcatgctgt gtttagtaat agaactcttg cttgctttgc tatttacacc
     9001 acaaaggaaa aagctgcact gctatacaag aaaattatgg aaaaatattc tgtaaccttt
     9061 ataagtaggc ataacagtta taatcataac atactgtttt ttcttactcc acacaggcat
     9121 agagtgtctg ctattaataa ctatgctcaa aaattgtgta cctttagctt tttaatttgt
     9181 aaaggggtta ataaggaata tttgatgtat agtgccttga ctagagatca taatcagcca
     9241 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
     9301 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
     9361 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     9421 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca
     9481 agctaaccaa aatcatccca aacttcccac cccataccct attaccactg ccaattacct
     9541 gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg
     9601 tatttgttaa atatgtacta caaacttagt agttggaagg gctaattcac tcccaaagaa
     9661 gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct gattagcaga
     9721 actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc tacaagctag
     9781 taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac
     9841 accctgtgag cctgcatggg atggatgacc cggagagaga agtgttagag tggaggtttg
     9901 acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac ttcaagaact
     9961 gctgatatcg agcttgctac aagggacttt ccgctgggga ctttccaggg aggcgtggcc
    10021 tgggcgggac tggggagtgg cgagccctca gatcctgcat ataagcagct gctttttgcc
    10081 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
    10141 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
    10201 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
    10261 tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc
    10321 gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga
    10381 gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca
    10441 gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa
    10501 agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag
    10561 ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac
    10621 catcccttca gacaggatca gaagaactta gatcattata taatacagta gcaaccctct
    10681 attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg
    10741 aagagcaaaa caaaagtaag accaccgcac agcaagcggc cggccgctga tcttcagacc
    10801 tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa
    10861 aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa
    10921 aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat
    10981 gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca
    11041 gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt
    11101 ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca
    11161 acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg
    11221 gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga
    11281 gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca
    11341 aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg
    11401 gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg
    11461 aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca
    11521 gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc
    11581 cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa
//
