LOCUS       sequence-437705-       12306 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of CBP IDR7 fragment fused to optogenetic protein 
            mCh-Cry2WT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12306)
  TITLE     Bose Lab CBP Expression Plasmids
REFERENCE   2  (bases 1 to 12306)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHR-IDR7-mCh-Cry2WT-NLS
FEATURES             Location/Qualifiers
     source          1..12306
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    43..160
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        313..720
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             2488..3981
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     CDS             4078..4098
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    4163..4751
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4216..4236)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4634..4645)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4754..4770)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4755..4771)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4861..5094
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(5119..5137)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5120..5137)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5427..5446)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5660..5679)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5779..5801
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5839..5857)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5925..6029
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6030..6098,6099..6890)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6248..6267)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7061..7649
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7550..7569
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7803..7820
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7890..7910)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7895..8224
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8075..8210
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9358..9423
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9553..9573
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9998..10132
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10035..10054)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10089..10108
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10300..10933
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10980..11105
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11602..11835
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12020..12064
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cggatctcga cggtatcgcc aaatggcagt attcatccac aattttaaaa gaaaaggggg
       61 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
      121 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
      181 cagagatcca gtttggatcg ataagcttga tatcgaattc ctgcagcccc gataaaataa
      241 aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc
      301 aagctagctg cagtaacgcc attttgcaag gcatggaaaa ataccaaacc aagaatagag
      361 aagttcagat caagggcggg tacatgaaaa tagctaacgt tgggccaaac aggatatctg
      421 cggtgagcag tttcggcccc ggcccggggc caagaacaga tggtcaccgc agtttcggcc
      481 ccggcccgag gccaagaaca gatggtcccc agatatggcc caaccctcag cagtttctta
      541 agacccatca gatgtttcca ggctccccca aggacctgaa atgaccctgc gccttatttg
      601 aattaaccaa tcagcctgct tctcgcttct gttcgcgcgc ttctgcttcc cgagctctat
      661 aaaagagctc acaacccctc actcggcgcg ccagtcctcc gacagactga gtcgcccggg
      721 ggggatctgg agctctcgag aattctcacg gtggccaatc agcccggcat gcagccccag
      781 cctggcctcc agtcccagcc cggcatgcaa ccccagcctg gcatgcacca gcagcccagc
      841 ctgcagaacc tgaatgccat gcaggctggc gtgccgcggc ccggtgtgcc tccacagcag
      901 caggcgatgg gaggcctgaa cccccagggc caggccttga acatcatgaa cccaggacac
      961 aaccccaaca tggcgagtat gaatccacag taccgagaaa tgttacggag gcagctgctg
     1021 cagcagcagc agcaacagca gcagcaacaa cagcagcaac agcagcagca gcaagggagt
     1081 gccggcatgg ctgggggcat ggcggggcac ggccagttcc agcagcctca aggacccgga
     1141 ggctacccac cggccatgca gcagcagcag cgcatgcagc agcatctccc cctccagggc
     1201 agctccatgg gccagatggc ggctcagatg ggacagcttg gccagatggg gcagccgggg
     1261 ctgggggcag acagcacccc caacatccag caagccctgc agcagcggat tctgcagcaa
     1321 cagcagatga agcagcagat tgggtcccca ggccagccga accccatgag cccccagcaa
     1381 cacatgctct caggacagcc acaggcctcg catctccctg gccagcagat cgccacgtcc
     1441 cttagtaacc aggtgcggtc tccagcccct gtccagtctc cacggcccca gtcccagcct
     1501 ccacattcca gcccgtcacc acggatacag ccccagcctt cgccacacca cgtctcaccc
     1561 cagactggtt ccccccaccc cggactcgca gtcaccatgg ccagctccat agatcaggga
     1621 cacttgggga accccgaaca gagtgcaatg ctcccccagc tgaacacccc cagcaggagt
     1681 gcgctgtcca gcgaactgtc cctggtcggg gacaccacgg gggacacgag tggagcaagg
     1741 caggtggaca gtggatccgg agctaccatg gtgtctaaag gcgaggagga taacatggct
     1801 ataatcaagg aatttatgag gttcaaggtg catatggagg gatctgtgaa cggtcacgag
     1861 ttcgaaatcg agggcgaagg cgaggggcga ccctatgaag ggacacagac ggccaaactg
     1921 aaggtgacca agggtggccc cctgcctttc gcctgggaca tcctgtcacc ccagtttatg
     1981 tacggctcta aggcttacgt taagcaccct gccgatattc cggactactt gaaactgagc
     2041 ttccctgaag ggttcaagtg ggaacgggta atgaattttg aggatggcgg tgtagtcaca
     2101 gttacccagg acagctctct tcaggacgga gaatttatct ataaggttaa acttcggggc
     2161 actaacttcc catcagacgg ccccgtgatg cagaagaaaa ctatgggctg ggaagccagc
     2221 tcagaacgca tgtatcccga ggacggagca ctgaagggag aaatcaagca gcgactcaag
     2281 ctgaaggatg ggggacatta tgatgctgag gtcaaaacca cctataaggc caagaaaccc
     2341 gttcagcttc ctggggcata caacgtgaat atcaaactgg atattacatc ccataacgaa
     2401 gactatacca tcgtggaaca gtacgagcgg gccgagggca gacatagcac agggggcatg
     2461 gatgaattgt acaaggcggc cacgcgtatg aagatggaca aaaagactat agtttggttt
     2521 agaagagacc taaggattga ggataatcct gcattagcag cagctgctca cgaaggatct
     2581 gtttttcctg tcttcatttg gtgtcctgaa gaagaaggac agttttatcc tggaagagct
     2641 tcaagatggt ggatgaaaca atcacttgct cacttatctc aatccttgaa ggctcttgga
     2701 tctgacctca ctttaatcaa aacccacaac acgatttcag cgatcttgga ttgtatccgc
     2761 gttaccggtg ctacaaaagt cgtctttaac cacctctatg atcctgtttc gttagttcgg
     2821 gaccataccg taaaggagaa gctggtggaa cgtgggatct ctgtgcaaag ctacaatgga
     2881 gatctattgt atgaaccgtg ggagatatac tgcgaaaagg gcaaaccttt tacgagtttc
     2941 aattcttact ggaagaaatg cttagatatg tcgattgaat ccgttatgct tcctcctcct
     3001 tggcggttga tgccaataac tgcagcggct gaagcgattt gggcgtgttc gattgaagaa
     3061 ctagggctgg agaatgaggc cgagaaaccg agcaatgcgt tgttaactag agcttggtct
     3121 ccaggatgga gcaatgctga taagttacta aatgagttca tcgagaagca gttgatagat
     3181 tatgcaaaga acagcaagaa agttgttggg aattctactt cactactttc tccgtatctc
     3241 catttcgggg aaataagcgt cagacacgtt ttccagtgtg cccggatgaa acaaattata
     3301 tgggcaagag ataagaacag tgaaggagaa gaaagtgcag atctttttct taggggaatc
     3361 ggtttaagag agtattctcg gtatatatgt ttcaacttcc cgtttactca cgagcaatcg
     3421 ttgttgagtc atcttcggtt tttcccttgg gatgctgatg ttgataagtt caaggcctgg
     3481 agacaaggca ggaccggtta tccgttggtg gatgccggaa tgagagagct ttgggctacc
     3541 ggatggatgc ataacagaat aagagtgatt gtttcaagct ttgctgtgaa gtttcttctc
     3601 cttccatgga aatggggaat gaagtatttc tgggatacac ttttggatgc tgatttggaa
     3661 tgtgacatcc ttggctggca gtatatctct gggagtatcc ccgatggcca cgagcttgat
     3721 cgcttggaca atcccgcgtt acaaggcgcc aaatatgacc cagaaggtga gtacataagg
     3781 caatggcttc ccgagcttgc gagattgcca actgaatgga tccatcatcc atgggacgct
     3841 cctttaaccg tactcaaagc ttctggtgtg gaactcggaa caaactatgc gaaacccatt
     3901 gtagacatcg acacagctcg tgagctacta gctaaagcta tttcaagaac ccgtgaagca
     3961 cagatcatga tcggagcagc agcccgggat ccaccggtcg ccaccggttc agggagcgga
     4021 tccggctcag ggagtgcggc cgcaactccc acctgcaaca tgcgtgacgg aggcggtcca
     4081 aaaaagaaga gaaaggtatg actgaggccg cgactctaga gtcgacctgc aggcatgcaa
     4141 gcttgatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac
     4201 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     4261 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     4321 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     4381 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     4441 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     4501 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
     4561 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     4621 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     4681 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     4741 cgcctccccg catcgatacc gtcgacctcg agggaattaa ttcgagctcg gtacctttaa
     4801 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     4861 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     4921 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     4981 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     5041 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagcatct
     5101 agaattaatt ccgtgtattc tatagtgtca cctaaatcgt atgtgtatga tacataaggt
     5161 tatgtattaa ttgtagccgc gttctaacga caatatgtac aagcctaatt gtgtagcatc
     5221 tggcttactg aagcagaccc tatcatctct ctcgtaaact gccgtcagag tcggtttggt
     5281 tggacgaacc ttctgagttt ctggtaacgc cgtcccgcac ccggaaatgg tcagcgaacc
     5341 aatcagcagg gtcatcgcta gccagatcct ctacgccgga cgcatcgtgg ccggcatcac
     5401 cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg
     5461 ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt
     5521 ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct
     5581 caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg
     5641 tcgaatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
     5701 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     5761 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     5821 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     5881 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     5941 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     6001 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     6061 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     6121 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     6181 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     6241 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     6301 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     6361 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     6421 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     6481 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     6541 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     6601 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     6661 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     6721 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     6781 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     6841 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     6901 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     6961 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     7021 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     7081 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     7141 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     7201 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     7261 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     7321 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     7381 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     7441 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     7501 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     7561 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     7621 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     7681 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     7741 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     7801 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     7861 cgcgttggcc gattcattaa tgcagctgtg gaatgtgtgt cagttagggt gtggaaagtc
     7921 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     7981 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     8041 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     8101 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     8161 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     8221 caaaaagctt ggacacaaga caggcttgcg agatatgttt gagaatacca ctttatcccg
     8281 cgtcagggag aggcagtgcg taaaaagacg cggactcatg tgaaatactg gtttttagtg
     8341 cgccagatct ctataatctc gcgcaaccta ttttcccctc gaacactttt taagccgtag
     8401 ataaacaggc tgggacactt cacatgagcg aaaaatacat cgtcacctgg gacatgttgc
     8461 agatccatgc acgtaaactc gcaagccgac tgatgccttc tgaacaatgg aaaggcatta
     8521 ttgccgtaag ccgtggcggt ctgtaccggg tgcgttactg gcgcgtgaac tgggtattcg
     8581 tcatgtcgat accgtttgta tttccagcta cgatcacgac aaccagcgcg agcttaaagt
     8641 gctgaaacgc gcagaaggcg atggcgaagg cttcatcgtt attgatgacc tggtggatac
     8701 cggtggtact gcggttgcga ttcgtgaaat gtatccaaaa gcgcactttg tcaccatctt
     8761 cgcaaaaccg gctggtcgtc cgctggttga tgactatgtt gttgatatcc cgcaagatac
     8821 ctggattgaa cagccgtggg atatgggcgt cgtattcgtc ccgccaatct ccggtcgcta
     8881 atcttttcaa cgcctggcac tgccgggcgt tgttcttttt aacttcaggc gggttacaat
     8941 agtttccagt aagtattctg gaggctgcat ccatgacaca ggcaaacctg agcgaaaccc
     9001 tgttcaaacc ccgctttaaa catcctgaaa cctcgacgct agtccgccgc tttaatcacg
     9061 gcgcacaacc gcctgtgcag tcggcccttg atggtaaaac catccctcac tggtatcgca
     9121 tgattaaccg tctgatgtgg atctggcgcg gcattgaccc acgcgaaatc ctcgacgtcc
     9181 aggcacgtat tgtgatgagc gatgccgaac gtaccgacga tgatttatac gatacggtga
     9241 ttggctaccg tggcggcaac tggatttatg agtgggcccc ggatctttgt gaaggaacct
     9301 tacttctgtg gtgtgacata attggacaaa ctacctacag agatttaaag ctctaaggta
     9361 aatataaaat ttttaagtgt ataatgtgtt aaactactga ttctaattgt ttgtgtattt
     9421 tagattccaa cctatggaac tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa
     9481 aacctgtttt gctcagaaga aatgccatct agtgatgatg aggctactgc tgactctcaa
     9541 cattctactc ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt tccttcagaa
     9601 ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt
     9661 tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa atattctgta
     9721 acctttataa gtaggcataa cagttataat cataacatac tgttttttct tactccacac
     9781 aggcatagag tgtctgctat taataactat gctcaaaaat tgtgtacctt tagcttttta
     9841 atttgtaaag gggttaataa ggaatatttg atgtatagtg ccttgactag agatcataat
     9901 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
     9961 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
    10021 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
    10081 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat
    10141 aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa
    10201 ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag
    10261 aaattgtatt tgttaaatat gtactacaaa cttagtagtt ggaagggcta attcactccc
    10321 aaagaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt
    10381 agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca
    10441 agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct
    10501 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga
    10561 ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca
    10621 agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc
    10681 gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt
    10741 tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
    10801 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
    10861 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
    10921 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
    10981 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
    11041 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
    11101 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
    11161 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
    11221 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
    11281 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
    11341 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
    11401 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccggc cgctgatctt
    11461 cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt
    11521 agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag
    11581 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag
    11641 cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat
    11701 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact
    11761 cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa
    11821 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt
    11881 gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg
    11941 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga
    12001 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag
    12061 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat
    12121 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt
    12181 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga
    12241 caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt
    12301 agtgaa
//
