LOCUS       sequence-437708-       11769 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of CBP IDR3 fragment fused to optogenetic protein 
            mCh-Cry2WT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11769)
  TITLE     Bose Lab CBP Expression Plasmids
REFERENCE   2  (bases 1 to 11769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHR-IDR3-mCh-Cry2WT-NLS
FEATURES             Location/Qualifiers
     source          1..11769
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    43..160
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        313..720
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             1951..3444
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     CDS             3541..3561
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    3626..4214
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3679..3699)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4097..4108)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4217..4233)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4218..4234)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4324..4557
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4582..4600)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4583..4600)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4890..4909)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5123..5142)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5242..5264
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5302..5320)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5388..5492
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5493..5561,5562..6353)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5711..5730)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6524..7112
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7013..7032
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7266..7283
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7353..7373)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7358..7687
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7538..7673
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8821..8886
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9016..9036
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9461..9595
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9498..9517)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9552..9571
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9763..10396
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10443..10568
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11065..11298
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11483..11527
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cggatctcga cggtatcgcc aaatggcagt attcatccac aattttaaaa gaaaaggggg
       61 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
      121 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
      181 cagagatcca gtttggatcg ataagcttga tatcgaattc ctgcagcccc gataaaataa
      241 aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc
      301 aagctagctg cagtaacgcc attttgcaag gcatggaaaa ataccaaacc aagaatagag
      361 aagttcagat caagggcggg tacatgaaaa tagctaacgt tgggccaaac aggatatctg
      421 cggtgagcag tttcggcccc ggcccggggc caagaacaga tggtcaccgc agtttcggcc
      481 ccggcccgag gccaagaaca gatggtcccc agatatggcc caaccctcag cagtttctta
      541 agacccatca gatgtttcca ggctccccca aggacctgaa atgaccctgc gccttatttg
      601 aattaaccaa tcagcctgct tctcgcttct gttcgcgcgc ttctgcttcc cgagctctat
      661 aaaagagctc acaacccctc actcggcgcg ccagtcctcc gacagactga gtcgcccggg
      721 ggggatctgg agctctcgag aattctcacg aaccaacaaa ccatcctggg gtctccagct
      781 agtggaattc aaaacacaat tggttctgtt ggcacagggc aacagaatgc cacttcttta
      841 agtaacccaa atcccataga ccccagctcc atgcagcgag cctatgctgc tctcggactc
      901 ccctacatga accagcccca gacgcagctg cagcctcagg ttcctggcca gcaaccagca
      961 cagcctcaaa cccaccagca gatgaggact ctcaaccccc tgggaaataa tccaatgaac
     1021 attccagcag gaggaataac aacagatcag cagcccccaa acttgatttc agaatcagct
     1081 cttccgactt ccctgggggc cacaaaccca ctgatgaacg atggctccaa ctctggtaac
     1141 attggaaccc tcagcactat accaacagca gctcctcctt ctagcaccgg tagtggagca
     1201 aggcaggtgg acagtggatc cggagctacc atggtgtcta aaggcgagga ggataacatg
     1261 gctataatca aggaatttat gaggttcaag gtgcatatgg agggatctgt gaacggtcac
     1321 gagttcgaaa tcgagggcga aggcgagggg cgaccctatg aagggacaca gacggccaaa
     1381 ctgaaggtga ccaagggtgg ccccctgcct ttcgcctggg acatcctgtc accccagttt
     1441 atgtacggct ctaaggctta cgttaagcac cctgccgata ttccggacta cttgaaactg
     1501 agcttccctg aagggttcaa gtgggaacgg gtaatgaatt ttgaggatgg cggtgtagtc
     1561 acagttaccc aggacagctc tcttcaggac ggagaattta tctataaggt taaacttcgg
     1621 ggcactaact tcccatcaga cggccccgtg atgcagaaga aaactatggg ctgggaagcc
     1681 agctcagaac gcatgtatcc cgaggacgga gcactgaagg gagaaatcaa gcagcgactc
     1741 aagctgaagg atgggggaca ttatgatgct gaggtcaaaa ccacctataa ggccaagaaa
     1801 cccgttcagc ttcctggggc atacaacgtg aatatcaaac tggatattac atcccataac
     1861 gaagactata ccatcgtgga acagtacgag cgggccgagg gcagacatag cacagggggc
     1921 atggatgaat tgtacaaggc ggccacgcgt atgaagatgg acaaaaagac tatagtttgg
     1981 tttagaagag acctaaggat tgaggataat cctgcattag cagcagctgc tcacgaagga
     2041 tctgtttttc ctgtcttcat ttggtgtcct gaagaagaag gacagtttta tcctggaaga
     2101 gcttcaagat ggtggatgaa acaatcactt gctcacttat ctcaatcctt gaaggctctt
     2161 ggatctgacc tcactttaat caaaacccac aacacgattt cagcgatctt ggattgtatc
     2221 cgcgttaccg gtgctacaaa agtcgtcttt aaccacctct atgatcctgt ttcgttagtt
     2281 cgggaccata ccgtaaagga gaagctggtg gaacgtggga tctctgtgca aagctacaat
     2341 ggagatctat tgtatgaacc gtgggagata tactgcgaaa agggcaaacc ttttacgagt
     2401 ttcaattctt actggaagaa atgcttagat atgtcgattg aatccgttat gcttcctcct
     2461 ccttggcggt tgatgccaat aactgcagcg gctgaagcga tttgggcgtg ttcgattgaa
     2521 gaactagggc tggagaatga ggccgagaaa ccgagcaatg cgttgttaac tagagcttgg
     2581 tctccaggat ggagcaatgc tgataagtta ctaaatgagt tcatcgagaa gcagttgata
     2641 gattatgcaa agaacagcaa gaaagttgtt gggaattcta cttcactact ttctccgtat
     2701 ctccatttcg gggaaataag cgtcagacac gttttccagt gtgcccggat gaaacaaatt
     2761 atatgggcaa gagataagaa cagtgaagga gaagaaagtg cagatctttt tcttagggga
     2821 atcggtttaa gagagtattc tcggtatata tgtttcaact tcccgtttac tcacgagcaa
     2881 tcgttgttga gtcatcttcg gtttttccct tgggatgctg atgttgataa gttcaaggcc
     2941 tggagacaag gcaggaccgg ttatccgttg gtggatgccg gaatgagaga gctttgggct
     3001 accggatgga tgcataacag aataagagtg attgtttcaa gctttgctgt gaagtttctt
     3061 ctccttccat ggaaatgggg aatgaagtat ttctgggata cacttttgga tgctgatttg
     3121 gaatgtgaca tccttggctg gcagtatatc tctgggagta tccccgatgg ccacgagctt
     3181 gatcgcttgg acaatcccgc gttacaaggc gccaaatatg acccagaagg tgagtacata
     3241 aggcaatggc ttcccgagct tgcgagattg ccaactgaat ggatccatca tccatgggac
     3301 gctcctttaa ccgtactcaa agcttctggt gtggaactcg gaacaaacta tgcgaaaccc
     3361 attgtagaca tcgacacagc tcgtgagcta ctagctaaag ctatttcaag aacccgtgaa
     3421 gcacagatca tgatcggagc agcagcccgg gatccaccgg tcgccaccgg ttcagggagc
     3481 ggatccggct cagggagtgc ggccgcaact cccacctgca acatgcgtga cggaggcggt
     3541 ccaaaaaaga agagaaaggt atgactgagg ccgcgactct agagtcgacc tgcaggcatg
     3601 caagcttgat atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt
     3661 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     3721 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     3781 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     3841 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     3901 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     3961 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     4021 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     4081 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     4141 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     4201 ggccgcctcc ccgcatcgat accgtcgacc tcgagggaat taattcgagc tcggtacctt
     4261 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     4321 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     4381 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     4441 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     4501 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagca
     4561 tctagaatta attccgtgta ttctatagtg tcacctaaat cgtatgtgta tgatacataa
     4621 ggttatgtat taattgtagc cgcgttctaa cgacaatatg tacaagccta attgtgtagc
     4681 atctggctta ctgaagcaga ccctatcatc tctctcgtaa actgccgtca gagtcggttt
     4741 ggttggacga accttctgag tttctggtaa cgccgtcccg cacccggaaa tggtcagcga
     4801 accaatcagc agggtcatcg ctagccagat cctctacgcc ggacgcatcg tggccggcat
     4861 caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga
     4921 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc
     4981 cgtggccggg ggactgttgg gcgccatctc cttgcatgca ccattccttg cggcggcggt
     5041 gctcaacggc ctcaacctac tactgggctg cttcctaatg caggagtcgc ataagggaga
     5101 gcgtcgaatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
     5161 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     5221 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     5281 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     5341 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     5401 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     5461 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     5521 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     5581 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     5641 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     5701 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     5761 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     5821 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     5881 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     5941 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     6001 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     6061 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     6121 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     6181 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     6241 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     6301 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     6361 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     6421 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     6481 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     6541 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     6601 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     6661 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     6721 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     6781 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     6841 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     6901 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     6961 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     7021 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     7081 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     7141 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     7201 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     7261 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     7321 ccgcgcgttg gccgattcat taatgcagct gtggaatgtg tgtcagttag ggtgtggaaa
     7381 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     7441 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     7501 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     7561 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     7621 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     7681 ttgcaaaaag cttggacaca agacaggctt gcgagatatg tttgagaata ccactttatc
     7741 ccgcgtcagg gagaggcagt gcgtaaaaag acgcggactc atgtgaaata ctggttttta
     7801 gtgcgccaga tctctataat ctcgcgcaac ctattttccc ctcgaacact ttttaagccg
     7861 tagataaaca ggctgggaca cttcacatga gcgaaaaata catcgtcacc tgggacatgt
     7921 tgcagatcca tgcacgtaaa ctcgcaagcc gactgatgcc ttctgaacaa tggaaaggca
     7981 ttattgccgt aagccgtggc ggtctgtacc gggtgcgtta ctggcgcgtg aactgggtat
     8041 tcgtcatgtc gataccgttt gtatttccag ctacgatcac gacaaccagc gcgagcttaa
     8101 agtgctgaaa cgcgcagaag gcgatggcga aggcttcatc gttattgatg acctggtgga
     8161 taccggtggt actgcggttg cgattcgtga aatgtatcca aaagcgcact ttgtcaccat
     8221 cttcgcaaaa ccggctggtc gtccgctggt tgatgactat gttgttgata tcccgcaaga
     8281 tacctggatt gaacagccgt gggatatggg cgtcgtattc gtcccgccaa tctccggtcg
     8341 ctaatctttt caacgcctgg cactgccggg cgttgttctt tttaacttca ggcgggttac
     8401 aatagtttcc agtaagtatt ctggaggctg catccatgac acaggcaaac ctgagcgaaa
     8461 ccctgttcaa accccgcttt aaacatcctg aaacctcgac gctagtccgc cgctttaatc
     8521 acggcgcaca accgcctgtg cagtcggccc ttgatggtaa aaccatccct cactggtatc
     8581 gcatgattaa ccgtctgatg tggatctggc gcggcattga cccacgcgaa atcctcgacg
     8641 tccaggcacg tattgtgatg agcgatgccg aacgtaccga cgatgattta tacgatacgg
     8701 tgattggcta ccgtggcggc aactggattt atgagtgggc cccggatctt tgtgaaggaa
     8761 ccttacttct gtggtgtgac ataattggac aaactaccta cagagattta aagctctaag
     8821 gtaaatataa aatttttaag tgtataatgt gttaaactac tgattctaat tgtttgtgta
     8881 ttttagattc caacctatgg aactgatgaa tgggagcagt ggtggaatgc ctttaatgag
     8941 gaaaacctgt tttgctcaga agaaatgcca tctagtgatg atgaggctac tgctgactct
     9001 caacattcta ctcctccaaa aaagaagaga aaggtagaag accccaagga ctttccttca
     9061 gaattgctaa gttttttgag tcatgctgtg tttagtaata gaactcttgc ttgctttgct
     9121 atttacacca caaaggaaaa agctgcactg ctatacaaga aaattatgga aaaatattct
     9181 gtaaccttta taagtaggca taacagttat aatcataaca tactgttttt tcttactcca
     9241 cacaggcata gagtgtctgc tattaataac tatgctcaaa aattgtgtac ctttagcttt
     9301 ttaatttgta aaggggttaa taaggaatat ttgatgtata gtgccttgac tagagatcat
     9361 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
     9421 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
     9481 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     9541 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg
     9601 gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc
     9661 caattacctg tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta
     9721 aagaaattgt atttgttaaa tatgtactac aaacttagta gttggaaggg ctaattcact
     9781 cccaaagaag acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg
     9841 attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt ggatggtgct
     9901 acaagctagt accagttgag ccagataagg tagaagaggc caataaagga gagaacacca
     9961 gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtgttagagt
    10021 ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat ccggagtact
    10081 tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac tttccaggga
    10141 ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg
    10201 ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
    10261 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
    10321 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
    10381 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
    10441 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
    10501 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
    10561 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
    10621 cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac
    10681 gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac
    10741 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag
    10801 caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca
    10861 agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc ggccgctgat
    10921 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
    10981 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
    11041 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
    11101 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
    11161 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
    11221 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
    11281 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
    11341 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
    11401 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
    11461 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
    11521 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
    11581 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
    11641 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
    11701 cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg
    11761 attagtgaa
//
