LOCUS       sequence-437710-       12981 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of CBP CFID fragment fused to optogenetic protein 
            mCh-Cry2WT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12981)
  TITLE     Bose Lab CBP Expression Plasmids
REFERENCE   2  (bases 1 to 12981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHR-CFID-mCh-Cry2WT-NLS
FEATURES             Location/Qualifiers
     source          1..12981
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    43..160
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        313..720
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             3163..4656
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     CDS             4753..4773
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    4838..5426
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4891..4911)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5309..5320)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5429..5445)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5430..5446)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5536..5769
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(5794..5812)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5795..5812)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6102..6121)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6335..6354)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6454..6476
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6514..6532)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6600..6704
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6705..6773,6774..7565)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6923..6942)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7736..8324
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8225..8244
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8478..8495
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8565..8585)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8570..8899
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8750..8885
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10033..10098
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10228..10248
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10673..10807
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10710..10729)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10764..10783
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10975..11608
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    11655..11780
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    12277..12510
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12695..12739
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cggatctcga cggtatcgcc aaatggcagt attcatccac aattttaaaa gaaaaggggg
       61 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
      121 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
      181 cagagatcca gtttggatcg ataagcttga tatcgaattc ctgcagcccc gataaaataa
      241 aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc
      301 aagctagctg cagtaacgcc attttgcaag gcatggaaaa ataccaaacc aagaatagag
      361 aagttcagat caagggcggg tacatgaaaa tagctaacgt tgggccaaac aggatatctg
      421 cggtgagcag tttcggcccc ggcccggggc caagaacaga tggtcaccgc agtttcggcc
      481 ccggcccgag gccaagaaca gatggtcccc agatatggcc caaccctcag cagtttctta
      541 agacccatca gatgtttcca ggctccccca aggacctgaa atgaccctgc gccttatttg
      601 aattaaccaa tcagcctgct tctcgcttct gttcgcgcgc ttctgcttcc cgagctctat
      661 aaaagagctc acaacccctc actcggcgcg ccagtcctcc gacagactga gtcgcccggg
      721 ggggatctgg agctctcgag aattctcacg acccccacac agcagcccag cacaccccag
      781 acgccgcagc cccctgccca gccccaaccc tcacccgtga gcatgtcacc agctggcttc
      841 cccagcgtgg cccggactca gccccccacc acggtgtcca cagggaagcc taccagccag
      901 gtgccggccc ccccaccccc ggcccagccc cctcctgcag cggtggaagc ggctcggcag
      961 atcgagcgtg aggcccagca gcagcagcac ctgtaccggg tgaacatcaa caacagcatg
     1021 cccccaggac gcacgggcat ggggaccccg gggagccaga tggcccccgt gagcctgaat
     1081 gtgccccgac ccaaccaggt gagcgggccc gtcatgccca gcatgcctcc cgggcagtgg
     1141 cagcaggcgc cccttcccca gcagcagccc atgccaggct tgcccaggcc tgtgatatcc
     1201 atgcaggccc aggcggccgt ggctgggccc cggatgccca gcgtgcagcc acccaggagc
     1261 atctcaccca gcgctctgca agacctgctg cggaccctga agtcgcccag ctcccctcag
     1321 cagcaacagc aggtgctgaa cattctcaaa tcaaacccgc agctaatggc agctttcatc
     1381 aaacagcgca cagccaagta cgtggccaat cagcccggca tgcagcccca gcctggcctc
     1441 cagtcccagc ccggcatgca accccagcct ggcatgcacc agcagcccag cctgcagaac
     1501 ctgaatgcca tgcaggctgg cgtgccgcgg cccggtgtgc ctccacagca gcaggcgatg
     1561 ggaggcctga acccccaggg ccaggccttg aacatcatga acccaggaca caaccccaac
     1621 atggcgagta tgaatccaca gtaccgagaa atgttacgga ggcagctgct gcagcagcag
     1681 cagcaacagc agcagcaaca acagcagcaa cagcagcagc agcaagggag tgccggcatg
     1741 gctgggggca tggcggggca cggccagttc cagcagcctc aaggacccgg aggctaccca
     1801 ccggccatgc agcagcagca gcgcatgcag cagcatctcc ccctccaggg cagctccatg
     1861 ggccagatgg cggctcagat gggacagctt ggccagatgg ggcagccggg gctgggggca
     1921 gacagcaccc ccaacatcca gcaagccctg cagcagcgga ttctgcagca acagcagatg
     1981 aagcagcaga ttgggtcccc aggccagccg aaccccatga gcccccagca acacatgctc
     2041 tcaggacagc cacaggcctc gcatctccct ggccagcaga tcgccacgtc ccttagtaac
     2101 caggtgcggt ctccagcccc tgtccagtct ccacggcccc agtcccagcc tccacattcc
     2161 agcccgtcac cacggataca gccccagcct tcgccacacc acgtctcacc ccagactggt
     2221 tccccccacc ccggactcgc agtcaccatg gccagctcca tagatcaggg acacttgggg
     2281 aaccccgaac agagtgcaat gctcccccag ctgaacaccc ccagcaggag tgcgctgtcc
     2341 agcgaactgt ccctggtcgg ggacaccacg ggggacacgc tagagaagtt tgtggagggc
     2401 ttgagtggag caaggcaggt ggacagtgga tccggagcta ccatggtgtc taaaggcgag
     2461 gaggataaca tggctataat caaggaattt atgaggttca aggtgcatat ggagggatct
     2521 gtgaacggtc acgagttcga aatcgagggc gaaggcgagg ggcgacccta tgaagggaca
     2581 cagacggcca aactgaaggt gaccaagggt ggccccctgc ctttcgcctg ggacatcctg
     2641 tcaccccagt ttatgtacgg ctctaaggct tacgttaagc accctgccga tattccggac
     2701 tacttgaaac tgagcttccc tgaagggttc aagtgggaac gggtaatgaa ttttgaggat
     2761 ggcggtgtag tcacagttac ccaggacagc tctcttcagg acggagaatt tatctataag
     2821 gttaaacttc ggggcactaa cttcccatca gacggccccg tgatgcagaa gaaaactatg
     2881 ggctgggaag ccagctcaga acgcatgtat cccgaggacg gagcactgaa gggagaaatc
     2941 aagcagcgac tcaagctgaa ggatggggga cattatgatg ctgaggtcaa aaccacctat
     3001 aaggccaaga aacccgttca gcttcctggg gcatacaacg tgaatatcaa actggatatt
     3061 acatcccata acgaagacta taccatcgtg gaacagtacg agcgggccga gggcagacat
     3121 agcacagggg gcatggatga attgtacaag gcggccacgc gtatgaagat ggacaaaaag
     3181 actatagttt ggtttagaag agacctaagg attgaggata atcctgcatt agcagcagct
     3241 gctcacgaag gatctgtttt tcctgtcttc atttggtgtc ctgaagaaga aggacagttt
     3301 tatcctggaa gagcttcaag atggtggatg aaacaatcac ttgctcactt atctcaatcc
     3361 ttgaaggctc ttggatctga cctcacttta atcaaaaccc acaacacgat ttcagcgatc
     3421 ttggattgta tccgcgttac cggtgctaca aaagtcgtct ttaaccacct ctatgatcct
     3481 gtttcgttag ttcgggacca taccgtaaag gagaagctgg tggaacgtgg gatctctgtg
     3541 caaagctaca atggagatct attgtatgaa ccgtgggaga tatactgcga aaagggcaaa
     3601 ccttttacga gtttcaattc ttactggaag aaatgcttag atatgtcgat tgaatccgtt
     3661 atgcttcctc ctccttggcg gttgatgcca ataactgcag cggctgaagc gatttgggcg
     3721 tgttcgattg aagaactagg gctggagaat gaggccgaga aaccgagcaa tgcgttgtta
     3781 actagagctt ggtctccagg atggagcaat gctgataagt tactaaatga gttcatcgag
     3841 aagcagttga tagattatgc aaagaacagc aagaaagttg ttgggaattc tacttcacta
     3901 ctttctccgt atctccattt cggggaaata agcgtcagac acgttttcca gtgtgcccgg
     3961 atgaaacaaa ttatatgggc aagagataag aacagtgaag gagaagaaag tgcagatctt
     4021 tttcttaggg gaatcggttt aagagagtat tctcggtata tatgtttcaa cttcccgttt
     4081 actcacgagc aatcgttgtt gagtcatctt cggtttttcc cttgggatgc tgatgttgat
     4141 aagttcaagg cctggagaca aggcaggacc ggttatccgt tggtggatgc cggaatgaga
     4201 gagctttggg ctaccggatg gatgcataac agaataagag tgattgtttc aagctttgct
     4261 gtgaagtttc ttctccttcc atggaaatgg ggaatgaagt atttctggga tacacttttg
     4321 gatgctgatt tggaatgtga catccttggc tggcagtata tctctgggag tatccccgat
     4381 ggccacgagc ttgatcgctt ggacaatccc gcgttacaag gcgccaaata tgacccagaa
     4441 ggtgagtaca taaggcaatg gcttcccgag cttgcgagat tgccaactga atggatccat
     4501 catccatggg acgctccttt aaccgtactc aaagcttctg gtgtggaact cggaacaaac
     4561 tatgcgaaac ccattgtaga catcgacaca gctcgtgagc tactagctaa agctatttca
     4621 agaacccgtg aagcacagat catgatcgga gcagcagccc gggatccacc ggtcgccacc
     4681 ggttcaggga gcggatccgg ctcagggagt gcggccgcaa ctcccacctg caacatgcgt
     4741 gacggaggcg gtccaaaaaa gaagagaaag gtatgactga ggccgcgact ctagagtcga
     4801 cctgcaggca tgcaagcttg atatcaagct tatcgataat caacctctgg attacaaaat
     4861 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     4921 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     4981 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     5041 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     5101 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     5161 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     5221 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct
     5281 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     5341 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     5401 gatctccctt tgggccgcct ccccgcatcg ataccgtcga cctcgaggga attaattcga
     5461 gctcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     5521 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     5581 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     5641 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     5701 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     5761 tctctagcag catctagaat taattccgtg tattctatag tgtcacctaa atcgtatgtg
     5821 tatgatacat aaggttatgt attaattgta gccgcgttct aacgacaata tgtacaagcc
     5881 taattgtgta gcatctggct tactgaagca gaccctatca tctctctcgt aaactgccgt
     5941 cagagtcggt ttggttggac gaaccttctg agtttctggt aacgccgtcc cgcacccgga
     6001 aatggtcagc gaaccaatca gcagggtcat cgctagccag atcctctacg ccggacgcat
     6061 cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac
     6121 cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat
     6181 ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct
     6241 tgcggcggcg gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc
     6301 gcataaggga gagcgtcgaa tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     6361 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     6421 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     6481 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
     6541 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     6601 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     6661 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     6721 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     6781 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     6841 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     6901 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     6961 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     7021 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     7081 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     7141 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     7201 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     7261 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     7321 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     7381 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     7441 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     7501 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     7561 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     7621 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     7681 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     7741 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     7801 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     7861 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     7921 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     7981 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     8041 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     8101 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     8161 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     8221 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     8281 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     8341 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     8401 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     8461 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     8521 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctgtggaatg tgtgtcagtt
     8581 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     8641 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     8701 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     8761 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     8821 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     8881 aggcctaggc ttttgcaaaa agcttggaca caagacaggc ttgcgagata tgtttgagaa
     8941 taccacttta tcccgcgtca gggagaggca gtgcgtaaaa agacgcggac tcatgtgaaa
     9001 tactggtttt tagtgcgcca gatctctata atctcgcgca acctattttc ccctcgaaca
     9061 ctttttaagc cgtagataaa caggctggga cacttcacat gagcgaaaaa tacatcgtca
     9121 cctgggacat gttgcagatc catgcacgta aactcgcaag ccgactgatg ccttctgaac
     9181 aatggaaagg cattattgcc gtaagccgtg gcggtctgta ccgggtgcgt tactggcgcg
     9241 tgaactgggt attcgtcatg tcgataccgt ttgtatttcc agctacgatc acgacaacca
     9301 gcgcgagctt aaagtgctga aacgcgcaga aggcgatggc gaaggcttca tcgttattga
     9361 tgacctggtg gataccggtg gtactgcggt tgcgattcgt gaaatgtatc caaaagcgca
     9421 ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg gttgatgact atgttgttga
     9481 tatcccgcaa gatacctgga ttgaacagcc gtgggatatg ggcgtcgtat tcgtcccgcc
     9541 aatctccggt cgctaatctt ttcaacgcct ggcactgccg ggcgttgttc tttttaactt
     9601 caggcgggtt acaatagttt ccagtaagta ttctggaggc tgcatccatg acacaggcaa
     9661 acctgagcga aaccctgttc aaaccccgct ttaaacatcc tgaaacctcg acgctagtcc
     9721 gccgctttaa tcacggcgca caaccgcctg tgcagtcggc ccttgatggt aaaaccatcc
     9781 ctcactggta tcgcatgatt aaccgtctga tgtggatctg gcgcggcatt gacccacgcg
     9841 aaatcctcga cgtccaggca cgtattgtga tgagcgatgc cgaacgtacc gacgatgatt
     9901 tatacgatac ggtgattggc taccgtggcg gcaactggat ttatgagtgg gccccggatc
     9961 tttgtgaagg aaccttactt ctgtggtgtg acataattgg acaaactacc tacagagatt
    10021 taaagctcta aggtaaatat aaaattttta agtgtataat gtgttaaact actgattcta
    10081 attgtttgtg tattttagat tccaacctat ggaactgatg aatgggagca gtggtggaat
    10141 gcctttaatg aggaaaacct gttttgctca gaagaaatgc catctagtga tgatgaggct
    10201 actgctgact ctcaacattc tactcctcca aaaaagaaga gaaaggtaga agaccccaag
    10261 gactttcctt cagaattgct aagttttttg agtcatgctg tgtttagtaa tagaactctt
    10321 gcttgctttg ctatttacac cacaaaggaa aaagctgcac tgctatacaa gaaaattatg
    10381 gaaaaatatt ctgtaacctt tataagtagg cataacagtt ataatcataa catactgttt
    10441 tttcttactc cacacaggca tagagtgtct gctattaata actatgctca aaaattgtgt
    10501 acctttagct ttttaatttg taaaggggtt aataaggaat atttgatgta tagtgccttg
    10561 actagagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
    10621 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
    10681 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
    10741 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
    10801 ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc
    10861 tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt
    10921 attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag tagttggaag
    10981 ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac cacacacaag
    11041 gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct
    11101 ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag
    11161 gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag
    11221 aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc
    11281 atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg
    11341 actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca
    11401 tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
    11461 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
    11521 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
    11581 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
    11641 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
    11701 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
    11761 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
    11821 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
    11881 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
    11941 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
    12001 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
    12061 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
    12121 ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
    12181 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
    12241 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
    12301 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
    12361 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
    12421 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
    12481 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
    12541 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
    12601 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
    12661 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
    12721 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
    12781 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
    12841 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
    12901 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga
    12961 cagatccatt cgattagtga a
//
