LOCUS       sequence-437713-       11333 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of SV40 nuclear localization signal (NLS) fused to 
            optogenetic protein mCh-Cry2WT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11333)
  TITLE     Bose Lab CBP Expression Plasmids
REFERENCE   2  (bases 1 to 11333)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHR-mCh-Cry2WT_NLS
FEATURES             Location/Qualifiers
     source          1..11333
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    43..160
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        313..720
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             1515..3008
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     CDS             3105..3125
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    3190..3778
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3243..3263)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3661..3672)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3781..3797)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3782..3798)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3888..4121
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4146..4164)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4147..4164)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4454..4473)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(4687..4706)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4806..4828
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4866..4884)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4952..5056
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5057..5125,5126..5917)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5275..5294)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6088..6676
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6577..6596
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6830..6847
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6917..6937)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6922..7251
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7102..7237
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8385..8450
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8580..8600
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9025..9159
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9062..9081)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9116..9135
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9327..9960
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10007..10132
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10629..10862
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11047..11091
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cggatctcga cggtatcgcc aaatggcagt attcatccac aattttaaaa gaaaaggggg
       61 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
      121 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
      181 cagagatcca gtttggatcg ataagcttga tatcgaattc ctgcagcccc gataaaataa
      241 aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc
      301 aagctagctg cagtaacgcc attttgcaag gcatggaaaa ataccaaacc aagaatagag
      361 aagttcagat caagggcggg tacatgaaaa tagctaacgt tgggccaaac aggatatctg
      421 cggtgagcag tttcggcccc ggcccggggc caagaacaga tggtcaccgc agtttcggcc
      481 ccggcccgag gccaagaaca gatggtcccc agatatggcc caaccctcag cagtttctta
      541 agacccatca gatgtttcca ggctccccca aggacctgaa atgaccctgc gccttatttg
      601 aattaaccaa tcagcctgct tctcgcttct gttcgcgcgc ttctgcttcc cgagctctat
      661 aaaagagctc acaacccctc actcggcgcg ccagtcctcc gacagactga gtcgcccggg
      721 ggggatctgg agctctcgag aattctcacg cgtcaagtgg agcaaggcag gtggacagtg
      781 gatccggagc taccatggtg tctaaaggcg aggaggataa catggctata atcaaggaat
      841 ttatgaggtt caaggtgcat atggagggat ctgtgaacgg tcacgagttc gaaatcgagg
      901 gcgaaggcga ggggcgaccc tatgaaggga cacagacggc caaactgaag gtgaccaagg
      961 gtggccccct gcctttcgcc tgggacatcc tgtcacccca gtttatgtac ggctctaagg
     1021 cttacgttaa gcaccctgcc gatattccgg actacttgaa actgagcttc cctgaagggt
     1081 tcaagtggga acgggtaatg aattttgagg atggcggtgt agtcacagtt acccaggaca
     1141 gctctcttca ggacggagaa tttatctata aggttaaact tcggggcact aacttcccat
     1201 cagacggccc cgtgatgcag aagaaaacta tgggctggga agccagctca gaacgcatgt
     1261 atcccgagga cggagcactg aagggagaaa tcaagcagcg actcaagctg aaggatgggg
     1321 gacattatga tgctgaggtc aaaaccacct ataaggccaa gaaacccgtt cagcttcctg
     1381 gggcatacaa cgtgaatatc aaactggata ttacatccca taacgaagac tataccatcg
     1441 tggaacagta cgagcgggcc gagggcagac atagcacagg gggcatggat gaattgtaca
     1501 aggcggccac gcgtatgaag atggacaaaa agactatagt ttggtttaga agagacctaa
     1561 ggattgagga taatcctgca ttagcagcag ctgctcacga aggatctgtt tttcctgtct
     1621 tcatttggtg tcctgaagaa gaaggacagt tttatcctgg aagagcttca agatggtgga
     1681 tgaaacaatc acttgctcac ttatctcaat ccttgaaggc tcttggatct gacctcactt
     1741 taatcaaaac ccacaacacg atttcagcga tcttggattg tatccgcgtt accggtgcta
     1801 caaaagtcgt ctttaaccac ctctatgatc ctgtttcgtt agttcgggac cataccgtaa
     1861 aggagaagct ggtggaacgt gggatctctg tgcaaagcta caatggagat ctattgtatg
     1921 aaccgtggga gatatactgc gaaaagggca aaccttttac gagtttcaat tcttactgga
     1981 agaaatgctt agatatgtcg attgaatccg ttatgcttcc tcctccttgg cggttgatgc
     2041 caataactgc agcggctgaa gcgatttggg cgtgttcgat tgaagaacta gggctggaga
     2101 atgaggccga gaaaccgagc aatgcgttgt taactagagc ttggtctcca ggatggagca
     2161 atgctgataa gttactaaat gagttcatcg agaagcagtt gatagattat gcaaagaaca
     2221 gcaagaaagt tgttgggaat tctacttcac tactttctcc gtatctccat ttcggggaaa
     2281 taagcgtcag acacgttttc cagtgtgccc ggatgaaaca aattatatgg gcaagagata
     2341 agaacagtga aggagaagaa agtgcagatc tttttcttag gggaatcggt ttaagagagt
     2401 attctcggta tatatgtttc aacttcccgt ttactcacga gcaatcgttg ttgagtcatc
     2461 ttcggttttt cccttgggat gctgatgttg ataagttcaa ggcctggaga caaggcagga
     2521 ccggttatcc gttggtggat gccggaatga gagagctttg ggctaccgga tggatgcata
     2581 acagaataag agtgattgtt tcaagctttg ctgtgaagtt tcttctcctt ccatggaaat
     2641 ggggaatgaa gtatttctgg gatacacttt tggatgctga tttggaatgt gacatccttg
     2701 gctggcagta tatctctggg agtatccccg atggccacga gcttgatcgc ttggacaatc
     2761 ccgcgttaca aggcgccaaa tatgacccag aaggtgagta cataaggcaa tggcttcccg
     2821 agcttgcgag attgccaact gaatggatcc atcatccatg ggacgctcct ttaaccgtac
     2881 tcaaagcttc tggtgtggaa ctcggaacaa actatgcgaa acccattgta gacatcgaca
     2941 cagctcgtga gctactagct aaagctattt caagaacccg tgaagcacag atcatgatcg
     3001 gagcagcagc ccgggatcca ccggtcgcca ccggttcagg gagcggatcc ggctcaggga
     3061 gtgcggccgc aactcccacc tgcaacatgc gtgacggagg cggtccaaaa aagaagagaa
     3121 aggtatgact gaggccgcga ctctagagtc gacctgcagg catgcaagct tgatatcaag
     3181 cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     3241 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     3301 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     3361 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     3421 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     3481 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     3541 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     3601 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     3661 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     3721 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat
     3781 cgataccgtc gacctcgagg gaattaattc gagctcggta cctttaagac caatgactta
     3841 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     3901 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
     3961 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     4021 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     4081 atccctcaga cccttttagt cagtgtggaa aatctctagc agcatctaga attaattccg
     4141 tgtattctat agtgtcacct aaatcgtatg tgtatgatac ataaggttat gtattaattg
     4201 tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg tagcatctgg cttactgaag
     4261 cagaccctat catctctctc gtaaactgcc gtcagagtcg gtttggttgg acgaaccttc
     4321 tgagtttctg gtaacgccgt cccgcacccg gaaatggtca gcgaaccaat cagcagggtc
     4381 atcgctagcc agatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt
     4441 gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc
     4501 gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg
     4561 ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac
     4621 ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg aatggtgcac
     4681 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
     4741 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     4801 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
     4861 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     4921 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     4981 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     5041 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     5101 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     5161 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     5221 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     5281 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     5341 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     5401 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     5461 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     5521 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     5581 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     5641 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     5701 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     5761 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     5821 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     5881 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     5941 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     6001 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     6061 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     6121 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     6181 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     6241 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     6301 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     6361 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     6421 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     6481 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     6541 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     6601 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     6661 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     6721 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     6781 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     6841 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     6901 tcattaatgc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     6961 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     7021 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     7081 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     7141 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     7201 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagcttgga
     7261 cacaagacag gcttgcgaga tatgtttgag aataccactt tatcccgcgt cagggagagg
     7321 cagtgcgtaa aaagacgcgg actcatgtga aatactggtt tttagtgcgc cagatctcta
     7381 taatctcgcg caacctattt tcccctcgaa cactttttaa gccgtagata aacaggctgg
     7441 gacacttcac atgagcgaaa aatacatcgt cacctgggac atgttgcaga tccatgcacg
     7501 taaactcgca agccgactga tgccttctga acaatggaaa ggcattattg ccgtaagccg
     7561 tggcggtctg taccgggtgc gttactggcg cgtgaactgg gtattcgtca tgtcgatacc
     7621 gtttgtattt ccagctacga tcacgacaac cagcgcgagc ttaaagtgct gaaacgcgca
     7681 gaaggcgatg gcgaaggctt catcgttatt gatgacctgg tggataccgg tggtactgcg
     7741 gttgcgattc gtgaaatgta tccaaaagcg cactttgtca ccatcttcgc aaaaccggct
     7801 ggtcgtccgc tggttgatga ctatgttgtt gatatcccgc aagatacctg gattgaacag
     7861 ccgtgggata tgggcgtcgt attcgtcccg ccaatctccg gtcgctaatc ttttcaacgc
     7921 ctggcactgc cgggcgttgt tctttttaac ttcaggcggg ttacaatagt ttccagtaag
     7981 tattctggag gctgcatcca tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg
     8041 ctttaaacat cctgaaacct cgacgctagt ccgccgcttt aatcacggcg cacaaccgcc
     8101 tgtgcagtcg gcccttgatg gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct
     8161 gatgtggatc tggcgcggca ttgacccacg cgaaatcctc gacgtccagg cacgtattgt
     8221 gatgagcgat gccgaacgta ccgacgatga tttatacgat acggtgattg gctaccgtgg
     8281 cggcaactgg atttatgagt gggccccgga tctttgtgaa ggaaccttac ttctgtggtg
     8341 tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt
     8401 taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct
     8461 atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac ctgttttgct
     8521 cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat tctactcctc
     8581 caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg ctaagttttt
     8641 tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac accacaaagg
     8701 aaaaagctgc actgctatac aagaaaatta tggaaaaata ttctgtaacc tttataagta
     8761 ggcataacag ttataatcat aacatactgt tttttcttac tccacacagg catagagtgt
     8821 ctgctattaa taactatgct caaaaattgt gtacctttag ctttttaatt tgtaaagggg
     8881 ttaataagga atatttgatg tatagtgcct tgactagaga tcataatcag ccataccaca
     8941 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     9001 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     9061 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     9121 ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac
     9181 caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt
     9241 catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt
     9301 taaatatgta ctacaaactt agtagttgga agggctaatt cactcccaaa gaagacaaga
     9361 tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc agaactacac
     9421 accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt
     9481 tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt
     9541 gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg
     9601 cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata
     9661 tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg
     9721 gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg
     9781 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     9841 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     9901 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     9961 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
    10021 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
    10081 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
    10141 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
    10201 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
    10261 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
    10321 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
    10381 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
    10441 aaacaaaagt aagaccaccg cacagcaagc ggccggccgc tgatcttcag acctggagga
    10501 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
    10561 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
    10621 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
    10681 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
    10741 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
    10801 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
    10861 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
    10921 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
    10981 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
    11041 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
    11101 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
    11161 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
    11221 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
    11281 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaa
//
