LOCUS       sequence-437714-       11706 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of CBP IDR4 fragment fused to optogenetic protein 
            mCh-Cry2WT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11706)
  TITLE     Bose Lab CBP Expression Plasmids
REFERENCE   2  (bases 1 to 11706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHR-IDR4-mCh-Cry2WT-NLS
FEATURES             Location/Qualifiers
     source          1..11706
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    43..160
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        313..720
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             1888..3381
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     CDS             3478..3498
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    3563..4151
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3616..3636)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4034..4045)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4154..4170)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4155..4171)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4261..4494
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4519..4537)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4520..4537)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4827..4846)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5060..5079)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5179..5201
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5239..5257)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5325..5429
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5430..5498,5499..6290)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5648..5667)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6461..7049
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6950..6969
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7203..7220
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7290..7310)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7295..7624
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7475..7610
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8758..8823
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8953..8973
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9398..9532
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9435..9454)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9489..9508
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9700..10333
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10380..10505
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11002..11235
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11420..11464
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cggatctcga cggtatcgcc aaatggcagt attcatccac aattttaaaa gaaaaggggg
       61 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
      121 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
      181 cagagatcca gtttggatcg ataagcttga tatcgaattc ctgcagcccc gataaaataa
      241 aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc
      301 aagctagctg cagtaacgcc attttgcaag gcatggaaaa ataccaaacc aagaatagag
      361 aagttcagat caagggcggg tacatgaaaa tagctaacgt tgggccaaac aggatatctg
      421 cggtgagcag tttcggcccc ggcccggggc caagaacaga tggtcaccgc agtttcggcc
      481 ccggcccgag gccaagaaca gatggtcccc agatatggcc caaccctcag cagtttctta
      541 agacccatca gatgtttcca ggctccccca aggacctgaa atgaccctgc gccttatttg
      601 aattaaccaa tcagcctgct tctcgcttct gttcgcgcgc ttctgcttcc cgagctctat
      661 aaaagagctc acaacccctc actcggcgcg ccagtcctcc gacagactga gtcgcccggg
      721 ggggatctgg agctctcgag aattctcacg caaaaagaac tagaagaaaa acggaggtcg
      781 cgtttacata aacaaggcat cttggggaac cagccagcct taccagcccc gggggctcag
      841 ccccctgtga ttccacaggc acaacctgtg agacctccaa atggacccct gtccctgcca
      901 gtgaatcgca tgcaagtttc tcaagggatg aattcattta accccatgtc cttggggaac
      961 gtccagttgc cacaagcacc catgggacct cgtgcagcct ccccaatgaa ccactctgtc
     1021 cagatgaaca gcatgggctc agtgccaggg atggccattt ctccttcccg aatgcctcag
     1081 cctccgaaca tgatgggtgc acacaccaac aacatgatgg cccaggcgag tggagcaagg
     1141 caggtggaca gtggatccgg agctaccatg gtgtctaaag gcgaggagga taacatggct
     1201 ataatcaagg aatttatgag gttcaaggtg catatggagg gatctgtgaa cggtcacgag
     1261 ttcgaaatcg agggcgaagg cgaggggcga ccctatgaag ggacacagac ggccaaactg
     1321 aaggtgacca agggtggccc cctgcctttc gcctgggaca tcctgtcacc ccagtttatg
     1381 tacggctcta aggcttacgt taagcaccct gccgatattc cggactactt gaaactgagc
     1441 ttccctgaag ggttcaagtg ggaacgggta atgaattttg aggatggcgg tgtagtcaca
     1501 gttacccagg acagctctct tcaggacgga gaatttatct ataaggttaa acttcggggc
     1561 actaacttcc catcagacgg ccccgtgatg cagaagaaaa ctatgggctg ggaagccagc
     1621 tcagaacgca tgtatcccga ggacggagca ctgaagggag aaatcaagca gcgactcaag
     1681 ctgaaggatg ggggacatta tgatgctgag gtcaaaacca cctataaggc caagaaaccc
     1741 gttcagcttc ctggggcata caacgtgaat atcaaactgg atattacatc ccataacgaa
     1801 gactatacca tcgtggaaca gtacgagcgg gccgagggca gacatagcac agggggcatg
     1861 gatgaattgt acaaggcggc cacgcgtatg aagatggaca aaaagactat agtttggttt
     1921 agaagagacc taaggattga ggataatcct gcattagcag cagctgctca cgaaggatct
     1981 gtttttcctg tcttcatttg gtgtcctgaa gaagaaggac agttttatcc tggaagagct
     2041 tcaagatggt ggatgaaaca atcacttgct cacttatctc aatccttgaa ggctcttgga
     2101 tctgacctca ctttaatcaa aacccacaac acgatttcag cgatcttgga ttgtatccgc
     2161 gttaccggtg ctacaaaagt cgtctttaac cacctctatg atcctgtttc gttagttcgg
     2221 gaccataccg taaaggagaa gctggtggaa cgtgggatct ctgtgcaaag ctacaatgga
     2281 gatctattgt atgaaccgtg ggagatatac tgcgaaaagg gcaaaccttt tacgagtttc
     2341 aattcttact ggaagaaatg cttagatatg tcgattgaat ccgttatgct tcctcctcct
     2401 tggcggttga tgccaataac tgcagcggct gaagcgattt gggcgtgttc gattgaagaa
     2461 ctagggctgg agaatgaggc cgagaaaccg agcaatgcgt tgttaactag agcttggtct
     2521 ccaggatgga gcaatgctga taagttacta aatgagttca tcgagaagca gttgatagat
     2581 tatgcaaaga acagcaagaa agttgttggg aattctactt cactactttc tccgtatctc
     2641 catttcgggg aaataagcgt cagacacgtt ttccagtgtg cccggatgaa acaaattata
     2701 tgggcaagag ataagaacag tgaaggagaa gaaagtgcag atctttttct taggggaatc
     2761 ggtttaagag agtattctcg gtatatatgt ttcaacttcc cgtttactca cgagcaatcg
     2821 ttgttgagtc atcttcggtt tttcccttgg gatgctgatg ttgataagtt caaggcctgg
     2881 agacaaggca ggaccggtta tccgttggtg gatgccggaa tgagagagct ttgggctacc
     2941 ggatggatgc ataacagaat aagagtgatt gtttcaagct ttgctgtgaa gtttcttctc
     3001 cttccatgga aatggggaat gaagtatttc tgggatacac ttttggatgc tgatttggaa
     3061 tgtgacatcc ttggctggca gtatatctct gggagtatcc ccgatggcca cgagcttgat
     3121 cgcttggaca atcccgcgtt acaaggcgcc aaatatgacc cagaaggtga gtacataagg
     3181 caatggcttc ccgagcttgc gagattgcca actgaatgga tccatcatcc atgggacgct
     3241 cctttaaccg tactcaaagc ttctggtgtg gaactcggaa caaactatgc gaaacccatt
     3301 gtagacatcg acacagctcg tgagctacta gctaaagcta tttcaagaac ccgtgaagca
     3361 cagatcatga tcggagcagc agcccgggat ccaccggtcg ccaccggttc agggagcgga
     3421 tccggctcag ggagtgcggc cgcaactccc acctgcaaca tgcgtgacgg aggcggtcca
     3481 aaaaagaaga gaaaggtatg actgaggccg cgactctaga gtcgacctgc aggcatgcaa
     3541 gcttgatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac
     3601 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     3661 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     3721 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     3781 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     3841 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     3901 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
     3961 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     4021 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     4081 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     4141 cgcctccccg catcgatacc gtcgacctcg agggaattaa ttcgagctcg gtacctttaa
     4201 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     4261 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     4321 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     4381 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     4441 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagcatct
     4501 agaattaatt ccgtgtattc tatagtgtca cctaaatcgt atgtgtatga tacataaggt
     4561 tatgtattaa ttgtagccgc gttctaacga caatatgtac aagcctaatt gtgtagcatc
     4621 tggcttactg aagcagaccc tatcatctct ctcgtaaact gccgtcagag tcggtttggt
     4681 tggacgaacc ttctgagttt ctggtaacgc cgtcccgcac ccggaaatgg tcagcgaacc
     4741 aatcagcagg gtcatcgcta gccagatcct ctacgccgga cgcatcgtgg ccggcatcac
     4801 cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg
     4861 ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt
     4921 ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct
     4981 caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg
     5041 tcgaatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
     5101 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     5161 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     5221 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     5281 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     5341 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     5401 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     5461 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     5521 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     5581 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     5641 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     5701 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     5761 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     5821 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     5881 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     5941 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     6001 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     6061 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     6121 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     6181 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     6241 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     6301 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     6361 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     6421 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     6481 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     6541 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     6601 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     6661 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     6721 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     6781 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     6841 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     6901 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     6961 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     7021 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     7081 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     7141 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     7201 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     7261 cgcgttggcc gattcattaa tgcagctgtg gaatgtgtgt cagttagggt gtggaaagtc
     7321 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     7381 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     7441 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     7501 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     7561 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     7621 caaaaagctt ggacacaaga caggcttgcg agatatgttt gagaatacca ctttatcccg
     7681 cgtcagggag aggcagtgcg taaaaagacg cggactcatg tgaaatactg gtttttagtg
     7741 cgccagatct ctataatctc gcgcaaccta ttttcccctc gaacactttt taagccgtag
     7801 ataaacaggc tgggacactt cacatgagcg aaaaatacat cgtcacctgg gacatgttgc
     7861 agatccatgc acgtaaactc gcaagccgac tgatgccttc tgaacaatgg aaaggcatta
     7921 ttgccgtaag ccgtggcggt ctgtaccggg tgcgttactg gcgcgtgaac tgggtattcg
     7981 tcatgtcgat accgtttgta tttccagcta cgatcacgac aaccagcgcg agcttaaagt
     8041 gctgaaacgc gcagaaggcg atggcgaagg cttcatcgtt attgatgacc tggtggatac
     8101 cggtggtact gcggttgcga ttcgtgaaat gtatccaaaa gcgcactttg tcaccatctt
     8161 cgcaaaaccg gctggtcgtc cgctggttga tgactatgtt gttgatatcc cgcaagatac
     8221 ctggattgaa cagccgtggg atatgggcgt cgtattcgtc ccgccaatct ccggtcgcta
     8281 atcttttcaa cgcctggcac tgccgggcgt tgttcttttt aacttcaggc gggttacaat
     8341 agtttccagt aagtattctg gaggctgcat ccatgacaca ggcaaacctg agcgaaaccc
     8401 tgttcaaacc ccgctttaaa catcctgaaa cctcgacgct agtccgccgc tttaatcacg
     8461 gcgcacaacc gcctgtgcag tcggcccttg atggtaaaac catccctcac tggtatcgca
     8521 tgattaaccg tctgatgtgg atctggcgcg gcattgaccc acgcgaaatc ctcgacgtcc
     8581 aggcacgtat tgtgatgagc gatgccgaac gtaccgacga tgatttatac gatacggtga
     8641 ttggctaccg tggcggcaac tggatttatg agtgggcccc ggatctttgt gaaggaacct
     8701 tacttctgtg gtgtgacata attggacaaa ctacctacag agatttaaag ctctaaggta
     8761 aatataaaat ttttaagtgt ataatgtgtt aaactactga ttctaattgt ttgtgtattt
     8821 tagattccaa cctatggaac tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa
     8881 aacctgtttt gctcagaaga aatgccatct agtgatgatg aggctactgc tgactctcaa
     8941 cattctactc ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt tccttcagaa
     9001 ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt
     9061 tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa atattctgta
     9121 acctttataa gtaggcataa cagttataat cataacatac tgttttttct tactccacac
     9181 aggcatagag tgtctgctat taataactat gctcaaaaat tgtgtacctt tagcttttta
     9241 atttgtaaag gggttaataa ggaatatttg atgtatagtg ccttgactag agatcataat
     9301 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
     9361 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
     9421 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     9481 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat
     9541 aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa
     9601 ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag
     9661 aaattgtatt tgttaaatat gtactacaaa cttagtagtt ggaagggcta attcactccc
     9721 aaagaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt
     9781 agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca
     9841 agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct
     9901 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga
     9961 ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca
    10021 agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc
    10081 gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt
    10141 tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
    10201 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
    10261 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
    10321 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
    10381 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
    10441 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
    10501 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
    10561 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
    10621 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
    10681 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
    10741 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
    10801 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccggc cgctgatctt
    10861 cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt
    10921 agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag
    10981 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag
    11041 cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat
    11101 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact
    11161 cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa
    11221 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt
    11281 gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg
    11341 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga
    11401 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag
    11461 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat
    11521 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt
    11581 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga
    11641 caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt
    11701 agtgaa
//
