LOCUS       sequence-437720-       10271 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  NKX2-1-GFP fusion protein lentiviral overexpression using an EF1a 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10271)
  AUTHORS   Pulice JL, Meyerson M
  TITLE     Dosage amplification dictates oncogenic regulation by the NKX2-1 
            lineage factor in lung adenocarcinoma.
  JOURNAL   bioRxiv [Preprint]. 2023 Oct 27:2023.10.26.563996. doi: 
            10.1101/2023.10.26.563996.
  PUBMED    37994369
REFERENCE   2  (bases 1 to 10271)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2023 Oct 27:2023.10.26.563996. doi: 
            10.1101/2023.10.26.563996."
COMMENT     Sequence Label: pLX-EF1a_NKX2-1_Short_V5-GFP
FEATURES             Location/Qualifiers
     source          1..10271
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1766..1976
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1788..1984
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1835..1970
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2016..2618
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2016..2035)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2512..2532
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2661..2778
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2916..4094
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3147..4085
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4042..4062
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    4114..4138
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5255..5279)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5287..5328
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6124..6712
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6177..6197)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6595..6606)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6784..7017
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7095..7216
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7132..7151)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7186..7205
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7256..7391
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7411..7430)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7412..7430)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7440..7457)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7440..7456)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7449..7471)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7598..8053
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7685..7704)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7895..7916
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8079..8183
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8184..8252,8253..9044)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRILYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8402..8421)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9215..9803
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9704..9723
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9957..9974
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10091..10112
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10127..10132,10133..10150,10151..10157)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10165..10181
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10170..10192
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10189..10205
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10189..10205
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10224..10244
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10226..10244
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggaccca tgcattgcat ctcaattagt cagcaaccag
     1741 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc gtctcaatta
     1801 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     1861 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     1921 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     1981 caaaaagctt tctagaggta ccaccggagc ttaccatgac cgagtacaag cccacggtgc
     2041 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg
     2101 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc
     2161 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
     2221 acggcgccgc cgtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
     2281 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
     2341 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
     2401 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
     2461 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
     2521 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
     2581 gcacctggtg catgacccgc aagcccggtg ccagttaacg aattctagat cttgagacaa
     2641 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg
     2701 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     2761 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccact ttggcgccgg
     2821 ctcgaggcct gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct
     2881 aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag
     2941 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
     3001 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
     3061 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
     3121 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
     3181 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga
     3241 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
     3301 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
     3361 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
     3421 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
     3481 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
     3541 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
     3601 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
     3661 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
     3721 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
     3781 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
     3841 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
     3901 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
     3961 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     4021 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     4081 ttcaggtgtc gtgaggctag catcgattga tcaacaagtt tgtacaaaaa agttggcacc
     4141 atgtcgatga gtccaaagca cacgactccg ttctcagtgt ctgacatctt gagtcccctg
     4201 gaggaaagct acaagaaagt gggcatggag ggcggcggcc tcggggctcc gctggcggcg
     4261 tacaggcagg gccaggcggc accgccaaca gcggccatgc agcagcacgc cgtggggcac
     4321 cacggcgccg tcaccgccgc ctaccacatg acggcggcgg gggtgcccca gctctcgcac
     4381 tccgccgtgg ggggctactg caacggcaac ctgggcaaca tgagcgagct gccgccgtac
     4441 caggacacca tgaggaacag cgcctctggc cccggatggt acggcgccaa cccagacccg
     4501 cgcttccccg ccatctcccg cttcatgggc ccggcgagcg gcatgaacat gagcggcatg
     4561 ggcggcctgg gctcgctggg ggacgtgagc aagaacatgg ccccgctgcc aagcgcgccg
     4621 cgcaggaagc gccgggtgct cttctcgcag gcgcaggtgt acgagctgga gcgacgcttc
     4681 aagcaacaga agtacctgtc ggcgccggag cgcgagcacc tggccagcat gatccacctg
     4741 acgcccacgc aggtcaagat ctggttccag aaccaccgct acaaaatgaa gcgccaggcc
     4801 aaggacaagg cggcgcagca gcaactgcag caggacagcg gcggcggcgg gggcggcggg
     4861 ggcaccgggt gcccgcagca gcaacaggct cagcagcagt cgccgcgacg cgtggcggtg
     4921 ccggtcctgg tgaaagacgg caaaccgtgc caggcgggtg cccccgcgcc gggcgccgcc
     4981 agcctacaag gccacgcgca gcagcaggcg cagcaccagg cgcaggccgc gcaggcggcg
     5041 gcagcggcca tctccgtggg cagcggtggc gccggccttg gcgcacaccc gggccaccag
     5101 ccaggcagcg caggccagtc tccggacctg gcgcaccacg ccgccagccc cgcggcgctg
     5161 cagggccagg tatccagcct gtcccacctg aactcctcgg gctcggacta cggcaccatg
     5221 tcctgctcca ccttgctata cggtcggacc tggttgccaa ctttcttgta caaagtggtt
     5281 accggtggta agcctatccc taaccctctc ctcggtctcg attctacgac gcgtgctgaa
     5341 gctgctgcaa aggaagctgc agctaaggag gctgcagcta aggctggatc cgtcagtaaa
     5401 ggggaagaat tgtttacggg tgttgttccg atacttgttg aattggatgg ggatgtgaat
     5461 ggtcataaat tttccgttag tggtgaaggg gaaggtgacg ctacttatgg taaattgaca
     5521 ttgaaattta tttgtactac aggaaaactt ccagtcccat ggccgacttt ggtaacgact
     5581 cttacatatg gagtccaatg ttttagtagg tatccagatc acatgaaaca acatgatttc
     5641 tttaaatcag caatgcctga gggatatgtt caagaacgaa caatattctt taaagatgat
     5701 gggaactata agacaagggc agaagtcaaa tttgaaggtg atacactcgt taatcggatt
     5761 gaactgaaag ggatagattt taaggaagat gggaatattc ttgggcacaa acttgaatat
     5821 aattataatt cccataatgt atacataatg gctgacaagc agaagaatgg aattaaagtc
     5881 aattttaaaa taaggcataa tatagaagat gggagtgtcc aattggctga tcattaccaa
     5941 caaaatacac ctattgggga tggtcctgtc ctcttgcctg ataatcatta tctttctaca
     6001 caatctgctc tctcaaagga ccctaatgaa aagcgcgacc atatggtgtt gttggaattt
     6061 gtcactgctg caggcattac catgggaatg gatgaactct ataaatgaac gcgttaagtc
     6121 gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     6181 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     6241 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     6301 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     6361 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     6421 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     6481 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     6541 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     6601 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     6661 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcgtcgactt
     6721 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     6781 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     6841 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     6901 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     6961 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     7021 cgtatagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     7081 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     7141 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     7201 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc
     7261 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     7321 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     7381 ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca
     7441 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     7501 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     7561 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
     7621 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     7681 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     7741 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     7801 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     7861 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     7921 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     7981 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     8041 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     8101 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     8161 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     8221 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     8281 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     8341 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     8401 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     8461 tactctattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     8521 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     8581 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     8641 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     8701 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     8761 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     8821 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     8881 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     8941 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     9001 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     9061 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     9121 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     9181 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     9241 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     9301 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     9361 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     9421 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     9481 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     9541 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     9601 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     9661 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     9721 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     9781 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     9841 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     9901 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     9961 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
    10021 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
    10081 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
    10141 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
    10201 atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc
    10261 tgcaagctta a
//
