LOCUS       sequence-437726-       10270 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To inducibly knockdown CDK12 expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10270)
  TITLE     Lee Lab shRNA knockdown vectors
REFERENCE   2  (bases 1 to 10270)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: LT3GEPIR-CDK12shRNA
FEATURES             Location/Qualifiers
     source          1..10270
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    148..169
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(184..189,190..207,208..214)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    222..238
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     227..249
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     246..262
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     246..262
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     281..301
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        283..301
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        329..555
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             556..736
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    783..908
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1401..1634
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1819..1863
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2130..2247
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2263..2641
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    2271..2289
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2307..2325
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2343..2361
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2379..2397
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2415..2433
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2451..2469
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2487..2505
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2550..2574
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(2665..2667,2668..2670,2671..3384)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2710..2731)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2971..2990)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3318..3339
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     ncRNA           3578..3592
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        3769..4268
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3804..3827)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4201..4220
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4289..4888
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4289..4308)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4785..4805
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4949..5516
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4966..5518
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5110..5127)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5337..5356
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(5530..6147,6148..6153,6154..6189,6190..6192,6193..6228,
                     6229..6231,6232..6267,6268..6276)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    6322..6910
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6375..6395)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6793..6804)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7117..7350
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7422..7543
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7459..7478)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7513..7532
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7583..7718
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7738..7757)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7739..7757)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7767..7784)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7767..7783)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7776..7798)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7925..8380
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8012..8031)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8222..8243
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8406..8510
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8511..8579,8580..9371)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8729..8748)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9542..10130
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10031..10050
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
       61 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
      121 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
      181 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
      241 tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag
      301 ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt gtagtcttgc
      361 aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc
      421 cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca gacgggtctg
      481 acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt taagtgccta
      541 gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga gctctctggc
      601 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
      661 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
      721 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
      781 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
      841 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
      901 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
      961 cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac
     1021 gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac
     1081 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag
     1141 caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca
     1201 agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc
     1261 agacctggag gaggagatat gagggacaat tggagaagtg aattatataa atataaagta
     1321 gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga
     1381 gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc
     1441 actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata
     1501 gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc
     1561 acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag
     1621 gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg
     1681 ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg
     1741 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
     1801 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt
     1861 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata
     1921 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
     1981 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac
     2041 aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta
     2101 gtgaacggat ctcgacggta tcggttaact tttaaaagaa aaggggggat tggggggtac
     2161 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
     2221 aaacaaatta caaaaattca aaattttatc gattctagat tcgagtttac tccctatcag
     2281 tgatagagaa cgtatgaaga gtttactccc tatcagtgat agagaacgta tgcagacttt
     2341 actccctatc agtgatagag aacgtataag gagtttactc cctatcagtg atagagaacg
     2401 tatgaccagt ttactcccta tcagtgatag agaacgtatc tacagtttac tccctatcag
     2461 tgatagagaa cgtatatcca gtttactccc tatcagtgat agagaacgta taagctttag
     2521 gcgtgtacgg tgggcgccta taaaagcaga gctcgtttag tgaaccgtca gatcgcctgg
     2581 agcaattcca caacactttt gtcttatacc aactttccgt accacttcct accctcgtaa
     2641 agtcgagctt gcgttggatc caccatggtg agcaagggcg aggagctgtt caccggggtg
     2701 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtctggc
     2761 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     2821 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
     2881 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     2941 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     3001 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     3061 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     3121 atcatggccg acaagcagaa gaacggcatc aaggcgaact tcaagatccg ccacaacatc
     3181 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     3241 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
     3301 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     3361 ggcatggacg agctgtacaa gtaatgacgt acggcggccg cctcgactag ggataacagg
     3421 gtaattgttt gaatgaggct tcagtacttt acagaatcgt tgcctgcaca tcttggaaac
     3481 acttgctggg attacttcga cttcttaacc caacagaagg ctcgagaagg tatattgctg
     3541 ttgacagtga gcgagcactg aaagaggaga ttgtttagtg aagccacaga tgtaaacaat
     3601 ctcctctttc agtgcgtgcc tactgcctac ttcaaggggt ctagaattcg agcaattatc
     3661 ttgtttacta aaactgaata ccttgctatc tctttgatac atttttacaa agctgaatta
     3721 aaatggtata aattaaatca cttttttcaa ttgacgcgta attctaccgg gtaggggagg
     3781 cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct
     3841 acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc
     3901 cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc
     3961 ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc
     4021 tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc
     4081 aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg
     4141 gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg
     4201 cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg
     4261 gcctttcgac ctgcagccca agcttaccat gaccgagtac aagcccacgg tgcgcctcgc
     4321 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
     4381 cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga
     4441 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
     4501 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
     4561 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
     4621 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
     4681 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
     4741 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta
     4801 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg
     4861 gtgcatgacc cgcaagcccg gtgcctgacg cccgccccac gacccgcagc gcccgaccga
     4921 aaggagcgca cgaccccatg catccaattc cgcccccccc ccctaacgtt actggccgaa
     4981 gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt
     5041 cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg
     5101 gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc
     5161 ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc
     5221 ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa
     5281 aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc
     5341 tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg
     5401 gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac
     5461 gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataatatg
     5521 gccacaacca tgagtagact ggacaagagc aaagtcataa acggagctct ggaattactc
     5581 aatggtgtcg gtatcgaagg cctgacgaca aggaaactcg ctcaaaagct gggagttgag
     5641 cagcctaccc tgtactggca cgtgaagaac aagcgggccc tgctcgatgc cctgccaatc
     5701 gagatgctgg acaggcatca tacccacttc tgccccctgg aaggcgagtc atggcaagac
     5761 tttctgcgga acaacgccaa gtcataccgc tgtgctctcc tctcacatcg cgacggggct
     5821 aaagtgcatc tcggcacccg cccaacagag aaacagtacg aaaccctgga aaatcagctc
     5881 gcgttcctgt gtcagcaagg cttctccctg gagaacgcac tgtacgctct gtccgccgtg
     5941 ggccacttta cactgggctg cgtattggag gaacaggagc atcaagtagc aaaagaggaa
     6001 agagagacac ctaccaccga ttctatgccc ccacttctga gacaagcaat tgagctgttc
     6061 gaccggcagg gagccgaacc tgccttcctt ttcggcctgg aactaatcat atgtggcctg
     6121 gagaaacagc taaagtgcga aagcggcggg ccgaccgacg cccttgacga ttttgactta
     6181 gacatgctcc cagccgatgc ccttgacgac tttgaccttg atatgctgcc tgctgacgct
     6241 cttgacgatt ttgaccttga catgctcccc gggtaatccg gactcgacct gcagccaagc
     6301 ttatcgaaac gcgtctggaa caatcaacct ctggattaca aaatttgtga aagattgact
     6361 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     6421 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     6481 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     6541 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     6601 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     6661 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg
     6721 acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     6781 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     6841 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     6901 gcctccccgc ctggaattaa ttctgcagtc gagacctaga aaaacatgga gcaatcacaa
     6961 gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg
     7021 aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg
     7081 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     7141 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     7201 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     7261 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     7321 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
     7381 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     7441 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     7501 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     7561 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     7621 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     7681 gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc
     7741 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
     7801 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     7861 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     7921 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     7981 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     8041 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     8101 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     8161 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     8221 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     8281 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     8341 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
     8401 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     8461 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     8521 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     8581 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     8641 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     8701 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     8761 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     8821 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     8881 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     8941 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     9001 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     9061 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     9121 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     9181 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     9241 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     9301 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     9361 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     9421 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     9481 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     9541 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     9601 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     9661 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     9721 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     9781 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     9841 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     9901 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     9961 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
    10021 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
    10081 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
    10141 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
    10201 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
    10261 gccgcagccg
//
