LOCUS       sequence-437727-        9806 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Caging at cytosolic N-ter of POI:  TMEM-PhoCl-rtta3mScarlet.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9806)
  AUTHORS   Kashyap P, Bertelli S, Cao F, Kostritskaia Y, Blank F, Srikanth NA, 
            Schlack-Leigers C, Saleppico R, Bierhuizen D, Lu X, Nickel W, 
            Campbell RE, Plested AJR, Stauber T, Taylor MJ, Ewers H
  TITLE     An optogenetic method for the controlled release of single 
            molecules.
  JOURNAL   Nat Methods. 2024 Mar 8. doi: 10.1038/s41592-024-02204-x.
  PUBMED    38459384
REFERENCE   2  (bases 1 to 9806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2024 Mar 8. doi: 10.1038/s41592-024-02204-x."
COMMENT     Sequence Label: pTMEM-PhoCl-Insert
FEATURES             Location/Qualifiers
     source          1..9806
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(148..194)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    309..430
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(346..365)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     400..419
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(1216..1920)
                     /codon_start=1
                     /product="reverse tetracycline transactivator 3"
                     /label=rtTA3
                     /note="activates expression from Tet-responsive promoters 
                     in the presence of doxycycline"
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPG"
     promoter        complement(3839..4041)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3868..3888)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4043..4346
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    4427..4475
                     /note="synthetic polyadenylation signal"
     terminator      4493..4539
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_feature    4544..4615
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(4767..4903)
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     CDS             complement(join(4916..5707,5708..5776))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5539..5558
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     polyA_signal    6042..6090
                     /note="synthetic polyadenylation signal"
     CDS             complement(6117..7145)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     promoter        complement(7149..8358)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     complement(7293..7312)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     rep_origin      8858..9446
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9347..9366
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    9672..9743
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(join(9788..9806,1..118))
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
ORIGIN
        1 aaggaggaca cgtatggaag tgggcaagtt ggggaagccg tatccgttgc tgaatctggc
       61 atatgtggga gtataagacg cgcagcgtcg catcaggcat ttttttctgc gccaatgccg
      121 gtagatagca atccggcccg aggggcacaa aaaacccctc aagacccgtt tagaggcccc
      181 aaggggttat gctacgatac cgatagagat gggccaatac cacatttgta gaggttttac
      241 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
      301 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
      361 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
      421 atgtatctta tcatgtctgt gagctgaagg tacgctgtat ctcagtcagt caagctagca
      481 gaggcctgcg gatccccgcg gttacttgta cagctcgtcc attccgcctg tagagtgtct
      541 gccttcgctc cgctcgtact gttccaccac ggtgtagtcc tcgttgtggc tggtgatgtc
      601 cagctttctg tccacgttgt aggcgccagg catctgcacg ggtttcttgg ccttgtaggt
      661 ggtcttgaag tcggccaggt atctgccgcc atccttcagt ctcagggcca tcttgatgtc
      721 gcccttcagc acgccatcct caggatacag tctctcggtg ctggcttccc agcccatggt
      781 ctttttctgc atcacggggc catcaggtgg aaagttggtg ccccgcagct tcactttgta
      841 gatcagggtg ccatcttcca ggcttgtatc ctgtgtcacg gtcacggctc cgccgtcctc
      901 gaagttcatc actctctccc acttgaagcc ctcggggaag ctctgcttgt agtagtcggg
      961 aatatcggca gggtgctttg tgaaggcccg gctgccgtac ataaactgag ggctcagaat
     1021 gtcccagctg aaaggcagag ggccgccttt ggtcactttc agcttggcgg tctgtgttcc
     1081 ctcataaggt ctgccctcgc cttcgccttc gatctcgaac tcgtggccgt tcatgctgcc
     1141 ttccatgtgc accttgaacc gcatgaactc tttgatcacg gcctcgcctt tagaaaccat
     1201 gccactaccg aattccccgg ggagcatgtc aaggtcaaaa tcgtcaaggg catcggctgg
     1261 gagcatgtct aagtcaaaat cgtcaagggc gtcggtcggc ccgccgcttt cgcactttag
     1321 ctgtttctcc aggccacata tgattagttc caggccgaaa aggaaggcag gttcggctcc
     1381 ctgccggtcg aacagctcaa ttgcttgtct cagaagtggg ggcatagaat cggtggtagg
     1441 tgtctctctt tcctcttttg ctacttgatg ctcctgttcc tccaatacgc agcccagtgt
     1501 aaagtggccc acggcggaca gagcgtacag tgcgttctcc agggagaagc cttgctgaca
     1561 caggaacgcg agctgatttt ccagggtttc gtactgtttc tctgttgggc gggtgccgag
     1621 atgcacttta gccccgtcgc gatgtgagag gagagcacag cggtatgact tggcgttgtt
     1681 ccgcagaaag tcttgccatg actcgccttc cagggggcag aagtgggtat gatgcctgtc
     1741 cagcatctcg attggcaggg catcgagcag ggcccgcttg ttcttcacgt gccagtacag
     1801 ggtaggctgc tcaactccca gcttttgagc gagtttcctt gtcgtcaggc cttcgatacc
     1861 gacaccattg agtaattcca gagctccgtt tatgactttg ctcttgtcca gtctagacat
     1921 gccactacca agctttgtgc cgcctccgcc tctagggtac tttgtgaaca cccggttgcc
     1981 gtagtggaag gcggtggtca gaatgtcgta ggcgaaaggc agaggggcgc cttctttcac
     2041 ttccaggtcg atggtctgga tgccctcaaa gggcttgccg ctgccttcgc cttcgatcac
     2101 aaaggcgtgg ccgttcacgt tgccttccat ccgcagcttg ttcttcatgt cgggcttgat
     2161 cacgctggtg attgtctctt cgcccttaga caccatgccg ccgctgccgc ctttgtacag
     2221 ctcgtccata gaatcggtgg aggtcttggc cacggcgtgc tcgtacagct tgactttgtt
     2281 gtagtccttg tcgtggctca ggatctcgat ccggtgatcc acgaagtggc agtcgggcag
     2341 cttcacgggt ttctgcttca ctttgtaggt ggtccggtag tcgcctctgt agtgtccgcc
     2401 gcctttcagc agcagcttca ttttcacgtc gcccttcagc acgccatctc gctcgtacat
     2461 cttctcggtg ctggcttccc agccaactgt tctcttctgc atcacggggc cgtttggagg
     2521 gaagttcgtg ccctgaaagt ggatcttgtt gatgaagctg tcgccttcca tggtgatgtc
     2581 gttggtggcg atacagatgc cgccgtcctc gtatgtcatg gatctctccc agctgtagcc
     2641 ctcggggaag ctctgcttga agtagtcggg tatgacctcg agatctgagt ccggacttgt
     2701 acacagcgtc gggggagctg cctcgaaggt gattagactg gattctgggg cagccccctt
     2761 ccctgggggt gtgggagctt tgtctgaggg cagggggctg tccaccttgg ccccagactc
     2821 ctcttcatca tcatccatgc tgggccagat ggactggtct tccactctct tcagccgctc
     2881 attgagtgcc ttcagggcca gttgccttct ccgctcggcg tcttgagggt ctgtgcctgg
     2941 caggctgatg gtgatggagg atggggcacc cacatcgtag cgcttcaccg tcttctggca
     3001 tatctttacc ttcaccagga ggctgtgcac caagttcgcc agcaaaccca ccacaggctg
     3061 caggatctca gggaagaaag tggcgaaagc aaagtggtca gccatgtccc ctcggccccg
     3121 gctatggcgc tggtagaagc gaagatatac ccaactggag agcagcccga agccatagga
     3181 agccagcgcc gggctctgga gcagcgtggc gagccgcagc aggagcagca gcgccagcag
     3241 cagcatgggc atcacactga cgcgcacctg gggcactcgc aggaccacac agtcccccat
     3301 ggtttgcttg agtgccacca ggacgccacc taggaagccc aaggcgccgt ggatacggac
     3361 agtgaacagg tagaccaggt tgaaggaagc catgtaggtg aggaggtagg cgaaggcccc
     3421 cagcagccct acagacacat tcaccactga gaagaagatg agcagctcca aggcccccca
     3481 gaggggctcc agcaaacgcc cggccaccac caccgttgtc aggctgatgg ccacgtccca
     3541 cacatgctgc tccatcagcc catgggtggc cagggtccag atccagaagt tgggaggaaa
     3601 gaggtagccc ggggtgaccg ccaggcagcc tgtgtccacg gcgaaggaga gcaggtagag
     3661 gaatagtacc gccgcacaca gagccttcac caccacgctg gcgctggcca gaatggcccc
     3721 caagtgctgg cgggcgcctg gcagggcacg ttgcatggtg gcgctagcgc ggccgctggc
     3781 gggtgtgtcg agtggatggt aggatcgaca aagatctgat ctgacggttc actaaaccag
     3841 ctctgcttat atagacctcc caccgtacac gcctaccgcc catttgcgtc aatggggcgg
     3901 agttgttacg acattttgga aagtcccgtt gattttggtg ccaaaacaaa ctcccattga
     3961 cgtcaatggg gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga
     4021 tgtactgcca aaaccgcatc agcatggtaa tagcgatgac taatacgtag atgtactgcc
     4081 aagtaggaaa gtcccataag gtcatgtact gggcataatg ccaggcgggc catttaccgt
     4141 cattgacgtc aatagggggc gtacttggca tatgatacac ttgatgtact gccaagtggg
     4201 cagtttaccg taaatactcc acccattgac gtcaatggaa agtccctatt ggcgttacta
     4261 tgggaacata cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg
     4321 ggccatttac cgtaagttat gtaacgcgga actccatata tgggctatga actaatgacc
     4381 ccgtaattga ttactagatc tggctacact cgatcagcag ttagataata aaatcgctat
     4441 ccatcgaaga tggatgtgtg ttggtttttt gtgtgtgtaa cgcaacgatt gatagcataa
     4501 ccccttgggg cctctaaacg ggtcttgagg ggttttttga attgagaggt agctgaagct
     4561 gctgttatga ccactagagg gaagaagata cctgtggagc taatggtcca agatgtcggg
     4621 agccgcgatc gctgccaaaa aggtaagctg acgatagctg acgagattcg atgcccgacg
     4681 agcaaatgag cgccacggct gttcgcgtaa gctcgaaacg acggagattc gatagagcca
     4741 gagtcgagtt taaacgattc gggatcttaa ggataaattt ctggtaagga ggacacgtat
     4801 ggaagtgggc aagttgggga agccgtatcc gttgctgaat ctggcatatg tgggagtata
     4861 agacgcgcag cgtcgcatca ggcatttttt tctgcgccaa tgcacctaga atcggttacc
     4921 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     4981 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     5041 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     5101 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     5161 tcaactgttg ccgggaagct agagtaagta gttcgccagt taatagttta cgcaaggttg
     5221 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     5281 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     5341 gctccttcgg tcctccaatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     5401 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     5461 ctggtgaata ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     5521 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     5581 ttggaaagcg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     5641 cgatgtaacc cactcgcgca cccaactgat cttcagcatc ttttactttc accagcgttt
     5701 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     5761 aatgttgaat actcatgcga aagcctcctg ctgatcctgt ctcttgatag atgtttgcaa
     5821 aagctaggac tcgattatac gttgaccgat cagactatca atatctaggt ttaaacagat
     5881 cggtacgctg ccgtgaccag gcttgagtac aatatctgac gattggactt cctgggtccc
     5941 aagctggatt ccgactggct gactgccaag caacgatatg gatcgtcgag actcgcactg
     6001 agaggatcgt tctcggtaca ccaggcgcgc ctagcgttac acacacaaaa aaccaacaca
     6061 catccatctt cgatggatag cgattttatt atctaagact aggcttacat actagcttat
     6121 tcctttgccc tcggacgagt gctggggcgt cggtttccac tatcggcgag tacttctaca
     6181 cagccatcgg tccagacggc ggcgcttctg cgggcgattt gtgtacgccc gacagtcccg
     6241 gctccggatc ggacgattgc gtcgcatcga ccctgcgccc aagctgcatc atcgaaattg
     6301 ccgtcaacca agctctgata gagttggtca agaccaatgc ggagcatata cgcccggagc
     6361 cggggcgatc ctgcaagctc cggatgcctc cgctcgaagt agcgcgtctg ctgctccata
     6421 caagccaacc acggcctcca gaagaagatg ttggcgacct cgtattggga atccccgaac
     6481 atcgcctcgc tccagtcaat gaccgctgtt atgcggccat tgtccgtcag gacattgttg
     6541 gagccgaaat ccgcgtgcac gaggtgccgg acttcggggc agtcctcggc ccaaagcatc
     6601 agctcatcga gagcctgcgc gacggacgca ctgacggtgt cgtccatcac agtttgccag
     6661 tgatacacat ggggatcagc aatcgcgcat atgaaatcac gccatgtagt gtattgaccg
     6721 attccttgcg gtccgaatgg gccgaacccg ctcgtctggc taagatcggc ggcagcgatg
     6781 gcatccattg cctccgcgac cggctgcaga acagcgggca gttcggtttc aggcaggtct
     6841 tgcaacgtga caccctgtgc acggcgggag atgcaatagg tcaggctctc gctgaactcc
     6901 ccaatgtcaa gcacttccgg aatcgggagc gcggcggatg caaagtgccg ataaacataa
     6961 cgatctttgt agaaaccatc ggcgcagcta tttacccgca ggacatatcc acgccctcct
     7021 acatcgaagc tgaaagcacg agattcttcg ccctccgaga gctgcatcag gtcggagacg
     7081 ctgtcgaact tttcgatcag aaacttctcg acagacgtcg cggtgagttc aggcttttta
     7141 cccatgttgt ctaacaaaaa agccaaaaac ggccagaatt tagcggacaa tttacaagtc
     7201 taacactgaa aattacatat tgacccaaat gattacattt caaaaggtgc ctaaaaaact
     7261 tcacaaaaca cactcgccaa ccccgagcgc atagttcaaa accggagctt cagctactta
     7321 agaagatagg tacataaaac cgaccaaaga aactgacgcc tcacttatcc ctcccctcac
     7381 cagaggtccg gcgcctgtcg attcaggaga gcctacccta ggcccgaacc ctgcgtcctg
     7441 cgacggagaa aagcctaccg cacacctacc ggcaggtggc cccaccctgc attataagcc
     7501 aacagaacgg gtgacgtcac gacacgacga gggcgcgcgc tcccaaaggt acgggtgcac
     7561 tgcccaacgg caccgccata actgccgccc ccgcaacaga cgacaaaccg agttctccag
     7621 tcagtgacaa acttcacgtc agggtcccca gatggtgccc agcccatctc acccgaataa
     7681 gagctttccc gcattagcga agggctcaag accttgggtt cttgccgccc accatgcccc
     7741 ccaccttgtt tcaacgacct cacagcccgc ctcacaagcg tcttccattc aagactcggg
     7801 aacagccgcc attttgctgc gctcccccca acccccagtt cagggcaacc ttgctcgcgg
     7861 acccagacta cagcccttgg cggtctctcc acacgcttcc gtcccaccga gcggcccggc
     7921 ggccacgaaa gccccggcca gcccagcagc ccgctactca ccaagtgacg atcacagcga
     7981 tccacaaaca agaaccgcga cccaaatccc ggctgcgacg gaactagctg tgccacaccc
     8041 ggcgcgtcct tatataatca tcggcgttca ccgccccacg gagatccctc cgcagaatcg
     8101 ccgagaaggg actacttttc ctcgcctgtt ccgctctctg gaaagaaaac cagtgcccta
     8161 gagtcaccca agtcccgtcc taaaatgtcc ttctgctgat actggggttc taaggccgag
     8221 tcttatgagc agcgggccgc tgtcctgagc gtccgggcgg aaggatcagg acgctcctgc
     8281 gcccttcgtc tgacgtggca gcgctcgccg tgacgagggg ggcgcccgcg ggaggcgcca
     8341 aaacccggcg cggaggccag gcgcgccccg atttcgatat tcctcggatc tcggatagat
     8401 cgggattgag gatgtaggtt gaacttctct cgggtgatcc cgatctcgga tattcgggat
     8461 tggagtaata cctggcgata ggcgctgcca agcaatagcc aaggcgcaag gccggccaag
     8521 agacttgcga tagttcgttc gagaggtagc ctgcaagatg gatcggacga aaggtttgaa
     8581 gattttccga cgagggcctt ggagagttcg gaaatttaaa tgcaactatg gatgaacgaa
     8641 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     8701 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     8761 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     8821 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     8881 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     8941 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     9001 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     9061 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     9121 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     9181 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     9241 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     9301 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     9361 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     9421 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     9481 ccttttgctg gccttttgct cacatgattt aaatgaaact cgtaatcgga gaacgggtgc
     9541 aatagcaagg accagatcga acttgactga cgcaattctg agcgtagtcg aaacccagac
     9601 aagtcgatct cgccaagtag ctgacttgac tggttaatta agtttcgata gcccaaggta
     9661 accaatgaga ttagggaggg aagaaagttt gatgaactac ttctgacccc tagtggtgtc
     9721 cagaaaagac cattaaagga atgtcttgat cgaaatcgag agatcgcctg caggatagct
     9781 gacgacctta aggataaatt tctggt
//
