LOCUS       sequence-437753-       10250 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  NKX2-1-GFP fusion protein lentiviral overexpression using an UBC 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10250)
  AUTHORS   Pulice JL, Meyerson M
  TITLE     Dosage amplification dictates oncogenic regulation by the NKX2-1 
            lineage factor in lung adenocarcinoma.
  JOURNAL   bioRxiv [Preprint]. 2023 Oct 27:2023.10.26.563996. doi: 
            10.1101/2023.10.26.563996.
  PUBMED    37994369
REFERENCE   2  (bases 1 to 10250)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2023 Oct 27:2023.10.26.563996. doi: 
            10.1101/2023.10.26.563996."
COMMENT     Sequence Label: pLX-UBC_NKX2-1_Short_V5-GFP
FEATURES             Location/Qualifiers
     source          1..10250
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1766..1976
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1788..1984
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1835..1970
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2016..2618
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2016..2035)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2512..2532
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2661..2778
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2852..4063
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3900..3919
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    4093..4117
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5234..5258)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5266..5307
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6103..6691
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6156..6176)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6574..6585)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6763..6996
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7074..7195
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7111..7130)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7165..7184
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7235..7370
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7390..7409)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7391..7409)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7419..7436)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7419..7435)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7428..7450)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7577..8032
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7664..7683)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7874..7895
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8058..8162
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8163..8231,8232..9023)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8381..8400)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9194..9782
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9683..9702
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9936..9953
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10070..10091
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10106..10111,10112..10129,10130..10136)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10144..10160
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10149..10171
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10168..10184
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10168..10184
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10203..10223
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10205..10223
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagatt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggaccca tgcattgcat ctcaattagt cagcaaccag
     1741 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc gtctcaatta
     1801 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     1861 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     1921 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     1981 caaaaagctt tctagaggta ccaccggagc ttaccatgac cgagtacaag cccacggtgc
     2041 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg
     2101 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc
     2161 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
     2221 acggcgccgc cgtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
     2281 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
     2341 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
     2401 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
     2461 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
     2521 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
     2581 gcacctggtg catgacccgc aagcccggtg ccagttaacg aattctagat cttgagacaa
     2641 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg
     2701 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     2761 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccact ttggcgccgg
     2821 ctcgaggggg cccgctctag acggttgatc tggcctccgc gccgggtttt ggcgcctccc
     2881 gcgggcgccc ccctcctcac ggcgagcgct gccacgtcag acgaagggcg cagcgagcgt
     2941 cctgatcctt ccgcccggac gctcaggaca gcggcccgct gctcataaga ctcggcctta
     3001 gaaccccagt atcagcagaa ggacatttta ggacgggact tgggtgactc tagggcactg
     3061 gttttctttc cagagagcgg aacaggcgag gaaaagtagt cccttctcgg cgattctgcg
     3121 gagggatctc cgtggggcgg tgaacgccga tgattatata aggacgcgcc gggtgtggca
     3181 cagctagttc cgtcgcagcc gggatttggg tcgcggttct tgtttgtgga tcgctgtgat
     3241 cgtcacttgg tgagtagcgg gctgctgggc tggccggggc tttcgtggcc gccgggccgc
     3301 tcggtgggac ggaagcgtgt ggagagaccg ccaagggctg tagtctgggt ccgcgagcaa
     3361 ggttgccctg aactgggggt tggggggagc gcagcaaaat ggcggctgtt cccgagtctt
     3421 gaatggaaga cgcttgtgag gcgggctgtg aggtcgttga aacaaggtgg ggggcatggt
     3481 gggcggcaag aacccaaggt cttgaggcct tcgctaatgc gggaaagctc ttattcgggt
     3541 gagatgggct ggggcaccat ctggggaccc tgacgtgaag tttgtcactg actggagaac
     3601 tcggtttgtc gtctgttgcg ggggcggcag ttatggcggt gccgttgggc agtgcacccg
     3661 tacctttggg agcgcgcgcc ctcgtcgtgt cgtgacgtca cccgttctgt tggcttataa
     3721 tgcagggtgg ggccacctgc cggtaggtgt gcggtaggct tttctccgtc gcaggacgca
     3781 gggttcgggc ctagggtagg ctctcctgaa tcgacaggcg ccggacctct ggtgagggga
     3841 gggataagtg aggcgtcagt ttctttggtc ggttttatgt acctatcttc ttaagtagct
     3901 gaagctccgg ttttgaacta tgcgctcggg gttggcgagt gtgttttgtg aagtttttta
     3961 ggcacctttt gaaatgtaat catttgggtc aatatgtaat tttcagtgtt agactagtaa
     4021 attgtccgct aaattctggc cgtttttggc ttttttgtta gacgaagcta acgcgctagc
     4081 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgtcgatgag tccaaagcac
     4141 acgactccgt tctcagtgtc tgacatcttg agtcccctgg aggaaagcta caagaaagtg
     4201 ggcatggagg gcggcggcct cggggctccg ctggcggcgt acaggcaggg ccaggcggca
     4261 ccgccaacag cggccatgca gcagcacgcc gtggggcacc acggcgccgt caccgccgcc
     4321 taccacatga cggcggcggg ggtgccccag ctctcgcact ccgccgtggg gggctactgc
     4381 aacggcaacc tgggcaacat gagcgagctg ccgccgtacc aggacaccat gaggaacagc
     4441 gcctctggcc ccggatggta cggcgccaac ccagacccgc gcttccccgc catctcccgc
     4501 ttcatgggcc cggcgagcgg catgaacatg agcggcatgg gcggcctggg ctcgctgggg
     4561 gacgtgagca agaacatggc cccgctgcca agcgcgccgc gcaggaagcg ccgggtgctc
     4621 ttctcgcagg cgcaggtgta cgagctggag cgacgcttca agcaacagaa gtacctgtcg
     4681 gcgccggagc gcgagcacct ggccagcatg atccacctga cgcccacgca ggtcaagatc
     4741 tggttccaga accaccgcta caaaatgaag cgccaggcca aggacaaggc ggcgcagcag
     4801 caactgcagc aggacagcgg cggcggcggg ggcggcgggg gcaccgggtg cccgcagcag
     4861 caacaggctc agcagcagtc gccgcgacgc gtggcggtgc cggtcctggt gaaagacggc
     4921 aaaccgtgcc aggcgggtgc ccccgcgccg ggcgccgcca gcctacaagg ccacgcgcag
     4981 cagcaggcgc agcaccaggc gcaggccgcg caggcggcgg cagcggccat ctccgtgggc
     5041 agcggtggcg ccggccttgg cgcacacccg ggccaccagc caggcagcgc aggccagtct
     5101 ccggacctgg cgcaccacgc cgccagcccc gcggcgctgc agggccaggt atccagcctg
     5161 tcccacctga actcctcggg ctcggactac ggcaccatgt cctgctccac cttgctatac
     5221 ggtcggacct ggttgccaac tttcttgtac aaagtggtta ccggtggtaa gcctatccct
     5281 aaccctctcc tcggtctcga ttctacgacg cgtgctgaag ctgctgcaaa ggaagctgca
     5341 gctaaggagg ctgcagctaa ggctggatcc gtcagtaaag gggaagaatt gtttacgggt
     5401 gttgttccga tacttgttga attggatggg gatgtgaatg gtcataaatt ttccgttagt
     5461 ggtgaagggg aaggtgacgc tacttatggt aaattgacat tgaaatttat ttgtactaca
     5521 ggaaaacttc cagtcccatg gccgactttg gtaacgactc ttacatatgg agtccaatgt
     5581 tttagtaggt atccagatca catgaaacaa catgatttct ttaaatcagc aatgcctgag
     5641 ggatatgttc aagaacgaac aatattcttt aaagatgatg ggaactataa gacaagggca
     5701 gaagtcaaat ttgaaggtga tacactcgtt aatcggattg aactgaaagg gatagatttt
     5761 aaggaagatg ggaatattct tgggcacaaa cttgaatata attataattc ccataatgta
     5821 tacataatgg ctgacaagca gaagaatgga attaaagtca attttaaaat aaggcataat
     5881 atagaagatg ggagtgtcca attggctgat cattaccaac aaaatacacc tattggggat
     5941 ggtcctgtcc tcttgcctga taatcattat ctttctacac aatctgctct ctcaaaggac
     6001 cctaatgaaa agcgcgacca tatggtgttg ttggaatttg tcactgctgc aggcattacc
     6061 atgggaatgg atgaactcta taaatgaacg cgttaagtcg acaatcaacc tctggattac
     6121 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     6181 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     6241 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     6301 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     6361 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     6421 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     6481 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     6541 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     6601 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     6661 agtcggatct ccctttgggc cgcctccccg cgtcgacttt aagaccaatg acttacaagg
     6721 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     6781 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     6841 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     6901 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     6961 tcagaccctt ttagtcagtg tggaaaatct ctagcagtac gtatagtagt tcatgtcatc
     7021 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     7081 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     7141 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     7201 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     7261 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     7321 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta
     7381 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
     7441 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     7501 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     7561 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     7621 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     7681 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     7741 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     7801 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     7861 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     7921 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     7981 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
     8041 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     8101 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     8161 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     8221 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     8281 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     8341 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     8401 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     8461 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     8521 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     8581 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     8641 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     8701 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     8761 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     8821 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     8881 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     8941 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     9001 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     9061 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     9121 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     9181 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     9241 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     9301 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     9361 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     9421 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     9481 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     9541 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     9601 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     9661 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     9721 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     9781 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     9841 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     9901 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     9961 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
    10021 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
    10081 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
    10141 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
    10201 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa
//
