LOCUS       sequence-437755-        6728 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Cre recombinase in cells lacking TDP-43 function. Note: It
            is not recommended to produce lentiviruses containing TDP-REG 
            sequences – see Depositor Comments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6728)
  AUTHORS   Wilkins OG, Chien MZYJ, Wlaschin JJ, Barattucci S, Harley P, Mattedi
            F, Mehta PR, Pisliakova M, Ryadnov E, Keuss MJ, Thompson D, Digby H,
            Knez L, Simkin RL, Diaz JA, Zanovello M, Brown AL, Darbey A, Karda 
            R, Fisher EMC, Cunningham TJ, Le Pichon CE, Ule J, Fratta P
  TITLE     Creation of de novo cryptic splicing for ALS and FTD precision 
            medicine.
  JOURNAL   Science. 2024 Oct 4;386(6717):61-69. doi: 10.1126/science.adk2539. 
            Epub 2024 Oct 3.
  PUBMED    39361759
REFERENCE   2  (bases 1 to 6728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.adk2539"; journalName: "Science"; date: "2024-10-4-
            4"; volume: "386"; issue: "6717"; pages: "61-69"
COMMENT     Sequence Label: TDP-REGv2 Triple Cryptic Cre recombinase in
            pT...
FEATURES             Location/Qualifiers
     source          1..6728
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        16..319
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        321..523
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     474..494
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             637..657
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2869..2895
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     polyA_signal    3190..3311
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3201..3220)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3255..3274
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      3426..3472
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      3502..3638
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    3683..3754
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     rep_origin      complement(3980..4568)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4060..4079)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(5314..5382,5383..6174)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5532..5551)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      6187..6323
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    6475..6546
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(6551..6597)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    6615..6663
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
       61 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
      121 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
      181 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
      241 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
      301 agtcatcgct attaccatgc tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
      361 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
      421 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
      481 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtca
      541 gatcagatct ttgtcgatcc taccatccac tcgacacacc cgccagcggc cgcttcttgg
      601 tgccagctta tcatagcgct accggtcgcc accatgccca agaagaagag gaaggtgtcc
      661 aacctgttaa cagtccacca gaacctacct gcccttcccg tcgacgctac gtcggacgag
      721 gttcggaaga acctgatgga catgttccgg gaccgacagg taagaatgca catcacttct
      781 tgagagtatg gaggagtgaa atgacactca gtgccagagt tactgtatat ctacacttta
      841 aaagtgtagc ttttaaaaga taagcaagca caatcttttg tgtgtgtgtg tgtgaatgtg
      901 tgtgtgtgtg tgtgtcaccc aggcatttag cgaacacacg tggaagatgc tgctttccgt
      961 ctgtcggagt tgggctgcgt ggtgcaagtt gaacaatcgt aaatggtttc cagccggtat
     1021 gcatcacccc cccagctaat ttttttttgt attttttacc gagtcggggt ttcgcaatgt
     1081 tgcccaggct ggtctcagag tctcgctctg ttgtctacgc tggagtgcag taacatgagc
     1141 cactgtgccc ggccaatcct aagaatttct tttgcggtgg ttgcaagtct gggcagaact
     1201 cttgtcaggg gctgtaactg gacttatctt tactcctttg tcagaaccgg aggacgtcag
     1261 agactatttg ctctacctgc aagcccgcgg actagccgtt aagactatac agcagcattt
     1321 gggacaatta aatatgttgc acaggtaaga atgcacatca cttcttgaga gtatggagga
     1381 gtgaaatgac actcagtgcc agagttactg tatatctaca ctttaaaagt gtagctttta
     1441 aaagataagc aagcacaatc ttttgtgtgt gtgtgtgtga atgtgtgtgt gtgtgtgtgt
     1501 cacccaggcg gtccgggctt ccccggcctt cggattcgaa cgcagtgagc ctagtcatgc
     1561 gccggattag aaaggaaaat gttgacgctg gagaacgggc aaagcaagta tgcatcaccc
     1621 ccccagctaa tttttttttg tattttttac cgagtcgggg tttcgcaatg ttgcccaggc
     1681 tggtctcaga gtctcgctct gttgtctacg ctggagtgca gtaacatgag ccactgtgcc
     1741 cggccaatcc taagaatttc ttttgcggtg gttgcaagtc tgggcagaac tcttgtcagg
     1801 ggctgtaact ggacttatct ttactccttt gtcaggcttt agcgtttgag agaacagatt
     1861 ttgatcaagt gcgatccctt atggagaact ctgaccgttg ccaagacata agaaatcttg
     1921 ctttcttggg catcgcgtac aacaccttac tgagaattgc ggagattgcc cggattcgag
     1981 tcaaggatat aagccgcacc gacggaggac ggatgctcat ccacattggg agaacgaaaa
     2041 ccctagtgtc aaccgccggt gtggagaaag ctctgagcct tggagtcaca aaattggtcg
     2101 agcgatggat caggtaagaa tgcacatcac ttcttgagag tatggaggag tgaaatgaca
     2161 ctcagtgcca gagttactgt atatctacac tttaaaagtg tagcttttaa aagataagca
     2221 agcacaatct tttgtgtgtg tgtgtgtgaa tgtgtgtgtg tgtgtgtgtc acccagcgtt
     2281 tccggtgtgg ccgacgatcc aaacaactat ctattctgtc gggtccgtaa aaatggggtt
     2341 gcggcaccaa gcgcaacctc ccagttgagc acgagggctc tggaaggtat gcatcacccc
     2401 cccagctaat ttttttttgt attttttacc gagtcggggt ttcgcaatgt tgcccaggct
     2461 ggtctcagag tctcgctctg ttgtctacgc tggagtgcag taacatgagc cactgtgccc
     2521 ggccaatcct aagaatttct tttgcggtgg ttgcaagtct gggcagaact cttgtcaggg
     2581 gctgtaactg gacttatctt tactcctttg tcaggtatct tcgaagccac tcaccgactg
     2641 atctacggcg caaaggacga ctccggacag cgataccttg cttggagtgg gcatagtgca
     2701 cgggtggggg cggctaggga tatggctaga gctggagtct caatccctga aattatgcaa
     2761 gctgggggtt ggacaaatgt taatattgta atgaactata taagaaactt ggatagtgag
     2821 acaggggcta tggtgcgcct gttagaagat ggggacggct ctggatctcc ggcggcgaaa
     2881 cgcgtgaaac tggattaaac aaatggtaag gaagggcaca tcaatctttg cttaattgtc
     2941 ctttactcta aagatgtatt ttatcatact gaatgctaaa cttgatatct ccttttaggt
     3001 cattgatgtc cttcaccccg ggaaggcgac agtgcctaag acagaaattc gggaaaaact
     3061 agccaaaatg tacaagacca caccggatgt catctttgta tttggattca gaactcagta
     3121 aactggatcc gcaggcctct gctagcttga ctgactgaga tacagcgtac cttcagctca
     3181 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa
     3241 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
     3301 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt
     3361 gggaggtttt ttaaagcaag taaaacctct acaaatgtgg tattggccca tctctatcgg
     3421 tatcgtagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgtgcccctc
     3481 gggccggatt gctatctacc ggcattggcg cagaaaaaaa tgcctgatgc gacgctgcgc
     3541 gtcttatact cccacatatg ccagattcag caacggatac ggcttcccca acttgcccac
     3601 ttccatacgt gtcctcctta ccagaaattt atccttaagg tcgtcagcta tcctgcaggc
     3661 gatctctcga tttcgatcaa gacattcctt taatggtctt ttctggacac cactaggggt
     3721 cagaagtagt tcatcaaact ttcttccctc cctaatctca ttggttacct tgggctatcg
     3781 aaacttaatt aaccagtcaa gtcagctact tggcgagatc gacttgtctg ggtttcgact
     3841 acgctcagaa ttgcgtcagt caagttcgat ctggtccttg ctattgcacc cgttctccga
     3901 ttacgagttt catttaaatc atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     3961 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     4021 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     4081 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     4141 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     4201 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     4261 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     4321 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     4381 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     4441 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     4501 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     4561 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     4621 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     4681 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     4741 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     4801 ttgcatttaa atttccgaac tctccaaggc cctcgtcgga aaatcttcaa acctttcgtc
     4861 cgatccatct tgcaggctac ctctcgaacg aactatcgca agtctcttgg ccggccttgc
     4921 gccttggcta ttgcttggca gcgcctatcg ccaggtatta ctccaatccc gaatatccga
     4981 gatcgggatc acccgagaga agttcaacct acatcctcaa tcccgatcta tccgagatcc
     5041 gaggaatatc gaaatcgggg cgcgcctggt gtaccgagaa cgatcctctc agtgcgagtc
     5101 tcgacgatcc atatcgttgc ttggcagtca gccagtcgga atccagcttg ggacccagga
     5161 agtccaatcg tcagatattg tactcaagcc tggtcacggc agcgtaccga tctgtttaaa
     5221 cctagatatt gatagtctga tcggtcaacg tataatcgag tcctagcttt tgcaaacatc
     5281 tatcaagaga caggatcagc aggaggcttt cgcatgagta ttcaacattt ccgtgtcgcc
     5341 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     5401 aaagtaaaag atgctgaaga tcagttgggt gcgcgagtgg gttacatcga actggatctc
     5461 aacagcggta agatccttga gagttttcgc cccgaagaac gctttccaat gatgagcact
     5521 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     5581 ggtcgccgca tacactattc tcagaatgac ttggttgagt attcaccagt cacagaaaag
     5641 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     5701 aacactgcgg ccaacttact tctgacaacg attggaggac cgaaggagct aaccgctttt
     5761 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     5821 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aaccttgcgt
     5881 aaactattaa ctggcgaact acttactcta gcttcccggc aacagttgat agactggatg
     5941 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     6001 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     6061 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     6121 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaaccgatt
     6181 ctaggtgcat tggcgcagaa aaaaatgcct gatgcgacgc tgcgcgtctt atactcccac
     6241 atatgccaga ttcagcaacg gatacggctt ccccaacttg cccacttcca tacgtgtcct
     6301 ccttaccaga aatttatcct taagatcccg aatcgtttaa actcgactct ggctctatcg
     6361 aatctccgtc gtttcgagct tacgcgaaca gccgtggcgc tcatttgctc gtcgggcatc
     6421 gaatctcgtc agctatcgtc agcttacctt tttggcagcg atcgcggctc ccgacatctt
     6481 ggaccattag ctccacaggt atcttcttcc ctctagtggt cataacagca gcttcagcta
     6541 cctctcaatt caaaaaaccc ctcaagaccc gtttagaggc cccaaggggt tatgctatca
     6601 atcgttgcgt tacacacaca aaaaaccaac acacatccat cttcgatgga tagcgatttt
     6661 attatctaac tgctgatcga gtgtagccag atctagtaat caattacggg gtcattagtt
     6721 catagccc
//
