LOCUS       sequence-437758-        8769 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  NKX2-1 short isoform lentiviral overexpression using hPGK promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8769)
  AUTHORS   Pulice JL, Meyerson M
  TITLE     Dosage amplification dictates oncogenic regulation by the NKX2-1 
            lineage factor in lung adenocarcinoma.
  JOURNAL   bioRxiv [Preprint]. 2023 Oct 27:2023.10.26.563996. doi: 
            10.1101/2023.10.26.563996.
  PUBMED    37994369
REFERENCE   2  (bases 1 to 8769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2023 Oct 27:2023.10.26.563996. doi: 
            10.1101/2023.10.26.563996."
COMMENT     Sequence Label: pLX-hPGK_NKX2-1
FEATURES             Location/Qualifiers
     source          1..8769
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1766..1976
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1788..1984
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1835..1970
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2016..2618
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2016..2035)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2512..2532
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2661..2778
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2836..3336
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3255..3273
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     protein_bind    3357..3381
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4498..4522)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4530..4571
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4622..5210
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4675..4695)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5093..5104)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5282..5515
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5593..5714
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5630..5649)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5684..5703
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5754..5889
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5909..5928)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5910..5928)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5938..5955)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5938..5954)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5947..5969)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6096..6551
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6183..6202)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6393..6414
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6577..6681
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6682..6750,6751..7542)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6900..6919)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7713..8301
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8202..8221
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8455..8472
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8589..8610
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8625..8630,8631..8648,8649..8655)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8663..8679
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8668..8690
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8687..8703
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8687..8703
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8722..8742
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8724..8742
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggaccca tgcattgcat ctcaattagt cagcaaccag
     1741 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc gtctcaatta
     1801 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     1861 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     1921 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     1981 caaaaagctt tctagaggta ccaccggagc ttaccatgac cgagtacaag cccacggtgc
     2041 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg
     2101 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc
     2161 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
     2221 acggcgccgc cgtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
     2281 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
     2341 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
     2401 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
     2461 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
     2521 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
     2581 gcacctggtg catgacccgc aagcccggtg ccagttaacg aattctagat cttgagacaa
     2641 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg
     2701 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     2761 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccact ttggcgccgg
     2821 ctcgaggcct gcaggttgcg ccttttccaa ggcagccctg ggtttgcgca gggacgcggc
     2881 tgctctgggc gtggttccgg gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc
     2941 acgtccgttc gcagcgtcac ccggatcttc gccgctaccc ttgtgggccc cccggcgacg
     3001 cttcctgctc cgcccctaag tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga
     3061 caaacggaag ccgcacgtct cactagtacc ctcgcagacg gacagcgcca gggagcaatg
     3121 gcagcgcgcc gaccgcgatg ggctgtggcc aatagcggct gctcagcagg gcgcgccgag
     3181 agcagcggcc gggaaggggc ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg
     3241 ttcctgcccg cgcggtgttc cgcattctgc aagcctccgg agcgcacgtc ggcagtcggc
     3301 tccctcgttg accgaatcac cgacctctct ccccaggggc tagcatcgat tgatcaacaa
     3361 gtttgtacaa aaaagttggc accatgtcga tgagtccaaa gcacacgact ccgttctcag
     3421 tgtctgacat cttgagtccc ctggaggaaa gctacaagaa agtgggcatg gagggcggcg
     3481 gcctcggggc tccgctggcg gcgtacaggc agggccaggc ggcaccgcca acagcggcca
     3541 tgcagcagca cgccgtgggg caccacggcg ccgtcaccgc cgcctaccac atgacggcgg
     3601 cgggggtgcc ccagctctcg cactccgccg tggggggcta ctgcaacggc aacctgggca
     3661 acatgagcga gctgccgccg taccaggaca ccatgaggaa cagcgcctct ggccccggat
     3721 ggtacggcgc caacccagac ccgcgcttcc ccgccatctc ccgcttcatg ggcccggcga
     3781 gcggcatgaa catgagcggc atgggcggcc tgggctcgct gggggacgtg agcaagaaca
     3841 tggccccgct gccaagcgcg ccgcgcagga agcgccgggt gctcttctcg caggcgcagg
     3901 tgtacgagct ggagcgacgc ttcaagcaac agaagtacct gtcggcgccg gagcgcgagc
     3961 acctggccag catgatccac ctgacgccca cgcaggtcaa gatctggttc cagaaccacc
     4021 gctacaaaat gaagcgccag gccaaggaca aggcggcgca gcagcaactg cagcaggaca
     4081 gcggcggcgg cgggggcggc gggggcaccg ggtgcccgca gcagcaacag gctcagcagc
     4141 agtcgccgcg acgcgtggcg gtgccggtcc tggtgaaaga cggcaaaccg tgccaggcgg
     4201 gtgcccccgc gccgggcgcc gccagcctac aaggccacgc gcagcagcag gcgcagcacc
     4261 aggcgcaggc cgcgcaggcg gcggcagcgg ccatctccgt gggcagcggt ggcgccggcc
     4321 ttggcgcaca cccgggccac cagccaggca gcgcaggcca gtctccggac ctggcgcacc
     4381 acgccgccag ccccgcggcg ctgcagggcc aggtatccag cctgtcccac ctgaactcct
     4441 cgggctcgga ctacggcacc atgtcctgct ccaccttgct atacggtcgg acctggttgc
     4501 caactttctt gtacaaagtg gttgatatcg gtaagcctat ccctaaccct ctcctcggtc
     4561 tcgattctac gtagtaatga actagtaccg gttaagtcga caatcaacgc gttaagtcga
     4621 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     4681 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     4741 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     4801 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     4861 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     4921 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     4981 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     5041 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     5101 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     5161 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc gtcgacttta
     5221 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     5281 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
     5341 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     5401 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     5461 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtacg
     5521 tatagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
     5581 gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     5641 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     5701 atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc
     5761 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     5821 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     5881 ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact
     5941 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     6001 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     6061 ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag
     6121 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     6181 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     6241 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     6301 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     6361 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     6421 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     6481 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     6541 gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     6601 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     6661 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     6721 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     6781 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     6841 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     6901 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     6961 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     7021 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     7081 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     7141 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     7201 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     7261 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     7321 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     7381 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     7441 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     7501 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     7561 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     7621 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     7681 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     7741 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     7801 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     7861 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     7921 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     7981 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     8041 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     8101 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     8161 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     8221 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     8281 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     8341 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     8401 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     8461 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     8521 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     8581 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     8641 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     8701 gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg
     8761 caagcttaa
//
