LOCUS       sequence-437760-        9639 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of CLIPf-KIF5A-SNAPf-HisTag-FLAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9639)
  AUTHORS   Smith ER, Turner ED, Abdelhamid MAS, Craggs TD, Twelvetrees AE
  TITLE     Kinesin-1 is highly flexible and adopts an open conformation in the 
            absence of cargo
  JOURNAL   bioRxiv 2024.12.20.629623
REFERENCE   2  (bases 1 to 9639)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.12.20.629623"
COMMENT     Sequence Label: CLIPf-KIF5A-SNAPf-HisTag-FLAG
FEATURES             Location/Qualifiers
     source          1..9639
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     37..57
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(66..494)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(176..197)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     388..407
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    540..764
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     753..770
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(818..841)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(842..859)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5118..5137)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5119..5137)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(5182..5385)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5211..5231)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5386..5765
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     5937..5956
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        6031..6135
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6136..6204,6205..6996)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6354..6373)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7167..7755
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7656..7675
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7909..7926
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8043..8064
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8079..8084,8085..8102,8103..8109)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8117..8133
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8122..8144
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8141..8157
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8141..8157
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    8206..8327
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8217..8236)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8271..8290
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(8501..9295)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8612..8631)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     9222..9241
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(join(9362..9639,1..52))
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9376..9511
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9430..9449)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
ORIGIN
        1 tacttctgcc tgctggggag cctggggact ttccacaccc taactgacac acattccaca
       61 gaattaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat
      121 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt
      181 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
      241 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag
      301 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg
      361 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc
      421 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
      481 gctacagggc gcgtggggat accccctaga gccccagctg gttctttccg cctcagaagc
      541 catagagccc accgcatccc cagcatgcct gctattgtct tcccaatcct cccccttgct
      601 gtcctgcccc accccacccc ccagaataga atgacaccta ctcagacaat gcgatgcaat
      661 ttcctcattt tattaggaaa ggacagtggg agtggcacct tccagggtca aggaaggcac
      721 gggggagggg caaacaacag atggctggca actagaaggc acagtcgagg ctgatcagcg
      781 ggtttaaacg ggccctctag actcgagcgg ccgctcattt gtcgtcgtcg tccttgtagt
      841 cgtggtgatg gtggtggtga aactcgccag atcctgctgc agatccggcg cttccaccaa
      901 gtccaggctt tccgagtcta tggccctcgt gagcaaggag ccactctttg acagccagtc
      961 cgccttcata tcctccaaca tccaggtcgc cctggacgac ccggtgacaa ggaatcagga
     1021 ttggcactgg attgccggac agggcagttt tcacagcggc agttgctgca ggatttccgg
     1081 ccagtgcagc caggtggctg taggagatca cttccccaaa tttcacgact ttcaggagtt
     1141 tccacaggac ttgccgtgtg aaggactctt gttgaaagac aggatggtgc agtgctggca
     1201 cgggaaactc ttcgatggct tccggctggt ggaaataagc attcagccaa gctgtagcct
     1261 gcataagtgg ctctggtcca ccaagcactg cagctggtgc tggaacttcc actgcatcag
     1321 cggcgctggt gccctttccg aggaatatga ttctgtgcag tccctgctcg catccagaca
     1381 gctcaagttt gcccagtggg gagtccaggg ttgtgcgctt catttcacaa tccttatcga
     1441 attcgctggc tgctgtctct tggtggagag ggaaaagctt ggcctggtcc tcagcctcat
     1501 agccacacgg caggtcactc ctgttgtcgt tgatatctgt ggcatttcca ttgtccatgt
     1561 tggccttctg gtaggaagcc aagggggcaa cagatgtggc tccgctgctg gcaaagtaca
     1621 tatctgaagt ggagctaggc gcagcctgca ggtgcaggtt ctggtagttc tggaagaggt
     1681 tgttggtgta gctgatacac tcggggctcc gggtgccgta ggggttggtg ggtgaggagg
     1741 caggatagtg gccaggcctc acaggcttag cgatctgggc agaatggccc cgcttgccgg
     1801 agctcttgta ccgcacggct tctttgatgc ggtccacctc ctgctggtat ctacgcttgt
     1861 ccttcatagc gccctccttg gcctccttca gtgcaccctc cagggcctta actctctcag
     1921 ccgtagcccg aagtcgtttt tccaatttag gaagctcaca acgcagatct gcattgtcac
     1981 gtaccagctg tttgtgaacc tttgtaagct gttccaggtt gttctcaaga aaggagatct
     2041 tctgcttttg ggaatgaatc cccccactgt cctcgggctc catttctgca cttttcttga
     2101 ctcgagtcgt gacgtcttga acgaacagct tgcgaaggtt gtggagggtc tggagttcac
     2161 gggcaactgt ctcctccagc cccttgaggt cctgcttgga ctgctcatgt cgctcataca
     2221 gaaatgtcag ctcctggagc ttggcgctct tctcgttctc ttcattcttc agcctctcat
     2281 agtcggcctg aagcttctcc agctctaact ggagcttctg gttcaggtct ttcagctcat
     2341 caatggtttt ctgtttctca ttaatctcat cccggaggcg ggccagctgc cggtgatggg
     2401 cctcacggtg attctccatc tgtagttcca gggccttctt cacctcctcc gcgtcctgtg
     2461 tgtctggctc cttgtctttc agagctacct cgtgtacagt ttcgtgcgcc tggagcctgg
     2521 caagctcatc gctcagggag tcgtaggact cttccaggtg ccgtttcttc aactccacag
     2581 tctgcatgta ctccgtgagt gaacggatct tggcctcatg ctgtgagatg agcagttggc
     2641 aagatgacag ctccctaccg gtcacctcca tcttgcgatg acactccacc tggaggttct
     2701 ccagctgccg acatcgctta accacggact tcacctccga cttgatcttg ctgatgtaga
     2761 gccgggccac cgtgaactcc tcctcgatgg ccccactgat ctccacgggc agcttaatct
     2821 cgccgttgcc cacgatgaca ctgaactcac tcaggtccct catcagccca ttcagcacct
     2881 cagcgatccg ctttcgctgg tgtccactga cctcctggag ccgctgtagc tcggactcca
     2941 gggacagcat ggtggccact ttctgggaca gctcgtccac cagcagctgg ttctgctggc
     3001 tcttctcctc cacctcctgg gacttctggt cgtagttgac cgccagctcc tctagggcct
     3061 gcagcacttc cttcacctcg tccttcgcag catcgttctc ggactgcagg tggctaagct
     3121 cccgctggac cttctcgttg tctccccgag tggacacgag cagctcttcc tggtccagca
     3181 tctgctgctt cagcttctca atgagctggc tctgctggtt gatctcatca tccttgtcat
     3241 caagctgctt gtagagacgg cggatctctt cctcatattt ctgcctttcc tcaggtgcga
     3301 tgcgtaccac aatggatgag ttgtcattca caggggtctc ctcgcagagc tcagctccca
     3361 gagctgagtc ctctccagcc aggcgctcag tctcaggcac attctctcca ttgcgccacc
     3421 ggctaagctc agcctctagc ttcgcaattg tctccttctg ggccttggtc ttctccttct
     3481 ccttctcata cttcttcttc cactgctcag cagtcagctc cagattcact gaggcagtgt
     3541 tcttgatggt cttcgcccgc tgtccaaaca tgagcgtgga cttggtctct gcgtcattgt
     3601 agctggacgg cgagcagcag atgaacatgg tagtcctgca gttccctccc agagagtcct
     3661 ggagaatcct cgtcattttg ctgtcgcggt acggcacgta gcttttggtg ccctctgcca
     3721 gtgcagagat cacgttcccc agggccgaca gcgacttgtt gatattcttt gcctcgtcca
     3781 gaacggctcc ctctgcccct gtcttgctga ccttctcgct tccggccaga tccacgaggt
     3841 acagcttccc gctgagcttc tgctcggtct ctacgttctc ctgcttgatg ttgatgagga
     3901 agatgctgtg gctccgagaa ctgtgctcgt tcatgttggt gacagctacg tgacggttgg
     3961 acttcccctc atcgatcaca tccagaatct cctctgggct ggacacaaag cgttcggtac
     4021 aacccttgac aaacggcacc cggtttttgt cctcatgcac ggacaggttc gtcttggtca
     4081 catccaaaag gtcacggatc ttatccaggt aaatctcgaa gtaagatacc ttaatgtgga
     4141 attcaaggtt ctcatccatg gagtagatgt ggttgaagat gtctcgagcg atccggggaa
     4201 tgatgcccat cagctgaggg tcgtgcagct tcccctccat ggtatgcgtt ttccctgagg
     4261 atgtctgtcc ataagcgaag attgtgccat tgtaaccagc aaggacgtct ttgacgatct
     4321 gcatggcaca ggcgtggtaa acctgctcct gagtggtgtt tggggggaag acgcggtcaa
     4381 agacatatgg ctttccccca ataatgacgc tgtcgtcccc ttggaaaatg gggatgaact
     4441 tgtccccccg cagaatctcg gcctggttca ggggccgaaa tcggcaaagc accttgatgc
     4501 tgcattcgtt gttagtctcc gccatggatc ctccgaggcc aggttttccc agtctgtgtc
     4561 cttcgtgggc cagcagccat tctttcacgg ccaggcctcc aagataaggg cccacatcag
     4621 aatcgccctg caccactctg tggcagggga tcagaatagg cacagggttt ccatccaggg
     4681 ctgtgttcac ggcggctgta gcagctggat ttcccacgag agcggccagg tgagactcgc
     4741 tgatcacttc gccgaacttg accaccttca gcagcttcca cagcacctgt ctggtgaagc
     4801 tttcttgctg gaacacgggg tgatgcagag caggaacggg gaactcctcg atggcctcgg
     4861 gctgatgaaa gtaggcgttc agccaggcgg tggcctgaat cagaggttca ggtcctccga
     4921 gcacagcagc aggagcaggc acttcaacag catcggcggc agaggttccc ttgccgagaa
     4981 agatgatccg gtgcaggccc tgttcacagc cgctcagttc cagcttgccc agagggctat
     5041 ccagtgtggt ccgcttcatc tcgcagtcct tgtccatggt accaagctta agtttaaacg
     5101 ctagccagct tgggtctccc tatagtgagt cgtattaatt tcgataagcc agtaagcagt
     5161 gggttctcta gttagccaga gagctctgct tatatagacc tcccaccgta cacgcctacc
     5221 gcccatttgc gtcaatgggg cggagttgtt acgacatttt ggaaagtccc gttgattttg
     5281 gtgccaaaac aaactcccat tgacgtcaat ggggtggaga cttggaaatc cccgtgagtc
     5341 aaaccgctat ccacgcccat tgatgtactg ccaaaaccgc atcaccatgg taatagcgat
     5401 gactaatacg tagatgtact gccaagtagg aaagtcccat aaggtcatgt actgggcata
     5461 atgccaggcg ggccatttac cgtcattgac gtcaataggg ggcgtacttg gcatatgata
     5521 cacttgatgt actgccaagt gggcagttta ccgtaaatac tccacccatt gacgtcaatg
     5581 gaaagtccct attggcgtta ctatgggaac atacgtcatt attgacgtca atgggcgggg
     5641 gtcgttgggc ggtcagccag gcgggccatt taccgtaagt tatgtaacgc ggaactccat
     5701 atatgggcta tgaactaatg accccgtaat tgattactat taataactag tcaataatca
     5761 atgtcaacgc gtatatctgg cccgtacatc gcgaagcagc gcaaaacgcc taaccctaag
     5821 cagattcttc atgcaattgt cggtcaagcc ttgccttgtt gtagcttaaa ttttgctcgc
     5881 gcactactca gcgacctcca acacacaagc agggagcaga tactggctta actatgcggc
     5941 atcagagcag attgtactga gagtgcacca taggggatcg ggagatctcc cgatccgtcg
     6001 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     6061 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     6121 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     6181 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     6241 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     6301 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     6361 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     6421 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     6481 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     6541 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     6601 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     6661 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     6721 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     6781 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     6841 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     6901 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     6961 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     7021 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     7081 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     7141 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     7201 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     7261 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     7321 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     7381 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     7441 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     7501 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     7561 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     7621 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     7681 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     7741 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     7801 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     7861 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     7921 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     7981 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     8041 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     8101 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
     8161 gattacgcca agctctagct agaggtcgac ggtatacaga catgataaga tacattgatg
     8221 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     8281 atgctattgc tttatttgta accattataa gctgcaataa acaagttggg gtgggcgaag
     8341 aactccagca tgagatcccc gcgctggagg atcatccagc cggcgtcccg gaaaacgatt
     8401 ccgaagccca acctttcata gaaggcggcg gtggaatcga aatctcgtga tggcaggttg
     8461 ggcgtcgctt ggtcggtcat ttcgaacccc agagtcccgc tcagaagaac tcgtcaagaa
     8521 ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc
     8581 ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac gctatgtcct
     8641 gatagcggtc cgccacaccc agccggccac agtcgatgaa tccagaaaag cggccatttt
     8701 ccaccatgat attcggcaag caggcatcgc catgggtcac gacgagatcc tcgccgtcgg
     8761 gcatgcgcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga tgctcttcgt
     8821 ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc tcgatgcgat
     8881 gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc cgccgcattg
     8941 catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg agatcctgcc
     9001 ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg tcgagcacag
     9061 ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg tcctgcagtt
     9121 cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca
     9181 gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca tagccgaata
     9241 gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa
     9301 acgatcctca tcctgtctct tgatcagatc cgaaaatgga tatacaagct cccgggagct
     9361 ttttgcaaaa gcctaggcct ccaaaaaagc ctcctcacta cttctggaat agctcagagg
     9421 cagaggcggc ctcggcctct gcataaataa aaaaaattag tcagccatgg ggcggagaat
     9481 gggcggaact gggcggagtt aggggcggga tgggcggagt taggggcggg actatggttg
     9541 ctgactaatt gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc
     9601 cacacctggt tgctgactaa ttgagatgca tgctttgca
//
