LOCUS       sequence-437766-        9514 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  NKX2-1-GFP fusion protein lentiviral overexpression using an hPGK 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9514)
  AUTHORS   Pulice JL, Meyerson M
  TITLE     Dosage amplification dictates oncogenic regulation by the NKX2-1 
            lineage factor in lung adenocarcinoma.
  JOURNAL   bioRxiv [Preprint]. 2023 Oct 27:2023.10.26.563996. doi: 
            10.1101/2023.10.26.563996.
  PUBMED    37994369
REFERENCE   2  (bases 1 to 9514)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2023 Oct 27:2023.10.26.563996. doi: 
            10.1101/2023.10.26.563996."
COMMENT     Sequence Label: pLX-hPGK_NKX2-1_Short_V5-GFP
FEATURES             Location/Qualifiers
     source          1..9514
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1766..1976
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1788..1984
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1835..1970
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2016..2618
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2016..2035)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2512..2532
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2661..2778
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2836..3336
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3255..3273
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     protein_bind    3357..3381
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4498..4522)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4530..4571
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5367..5955
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5420..5440)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5838..5849)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6027..6260
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6338..6459
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6375..6394)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6429..6448
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6499..6634
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6654..6673)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6655..6673)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6683..6700)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6683..6699)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6692..6714)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6841..7296
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6928..6947)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7138..7159
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7322..7426
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7427..7495,7496..8287)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRILYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7645..7664)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8458..9046
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8947..8966
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9200..9217
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9334..9355
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9370..9375,9376..9393,9394..9400)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9408..9424
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9413..9435
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9432..9448
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9432..9448
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9467..9487
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9469..9487
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggaccca tgcattgcat ctcaattagt cagcaaccag
     1741 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc gtctcaatta
     1801 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     1861 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     1921 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     1981 caaaaagctt tctagaggta ccaccggagc ttaccatgac cgagtacaag cccacggtgc
     2041 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg
     2101 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc
     2161 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
     2221 acggcgccgc cgtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
     2281 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
     2341 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
     2401 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
     2461 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
     2521 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
     2581 gcacctggtg catgacccgc aagcccggtg ccagttaacg aattctagat cttgagacaa
     2641 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg
     2701 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     2761 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccact ttggcgccgg
     2821 ctcgaggcct gcaggttgcg ccttttccaa ggcagccctg ggtttgcgca gggacgcggc
     2881 tgctctgggc gtggttccgg gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc
     2941 acgtccgttc gcagcgtcac ccggatcttc gccgctaccc ttgtgggccc cccggcgacg
     3001 cttcctgctc cgcccctaag tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga
     3061 caaacggaag ccgcacgtct cactagtacc ctcgcagacg gacagcgcca gggagcaatg
     3121 gcagcgcgcc gaccgcgatg ggctgtggcc aatagcggct gctcagcagg gcgcgccgag
     3181 agcagcggcc gggaaggggc ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg
     3241 ttcctgcccg cgcggtgttc cgcattctgc aagcctccgg agcgcacgtc ggcagtcggc
     3301 tccctcgttg accgaatcac cgacctctct ccccaggggc tagcatcgat tgatcaacaa
     3361 gtttgtacaa aaaagttggc accatgtcga tgagtccaaa gcacacgact ccgttctcag
     3421 tgtctgacat cttgagtccc ctggaggaaa gctacaagaa agtgggcatg gagggcggcg
     3481 gcctcggggc tccgctggcg gcgtacaggc agggccaggc ggcaccgcca acagcggcca
     3541 tgcagcagca cgccgtgggg caccacggcg ccgtcaccgc cgcctaccac atgacggcgg
     3601 cgggggtgcc ccagctctcg cactccgccg tggggggcta ctgcaacggc aacctgggca
     3661 acatgagcga gctgccgccg taccaggaca ccatgaggaa cagcgcctct ggccccggat
     3721 ggtacggcgc caacccagac ccgcgcttcc ccgccatctc ccgcttcatg ggcccggcga
     3781 gcggcatgaa catgagcggc atgggcggcc tgggctcgct gggggacgtg agcaagaaca
     3841 tggccccgct gccaagcgcg ccgcgcagga agcgccgggt gctcttctcg caggcgcagg
     3901 tgtacgagct ggagcgacgc ttcaagcaac agaagtacct gtcggcgccg gagcgcgagc
     3961 acctggccag catgatccac ctgacgccca cgcaggtcaa gatctggttc cagaaccacc
     4021 gctacaaaat gaagcgccag gccaaggaca aggcggcgca gcagcaactg cagcaggaca
     4081 gcggcggcgg cgggggcggc gggggcaccg ggtgcccgca gcagcaacag gctcagcagc
     4141 agtcgccgcg acgcgtggcg gtgccggtcc tggtgaaaga cggcaaaccg tgccaggcgg
     4201 gtgcccccgc gccgggcgcc gccagcctac aaggccacgc gcagcagcag gcgcagcacc
     4261 aggcgcaggc cgcgcaggcg gcggcagcgg ccatctccgt gggcagcggt ggcgccggcc
     4321 ttggcgcaca cccgggccac cagccaggca gcgcaggcca gtctccggac ctggcgcacc
     4381 acgccgccag ccccgcggcg ctgcagggcc aggtatccag cctgtcccac ctgaactcct
     4441 cgggctcgga ctacggcacc atgtcctgct ccaccttgct atacggtcgg acctggttgc
     4501 caactttctt gtacaaagtg gttaccggtg gtaagcctat ccctaaccct ctcctcggtc
     4561 tcgattctac gacgcgtgct gaagctgctg caaaggaagc tgcagctaag gaggctgcag
     4621 ctaaggctgg atccgtcagt aaaggggaag aattgtttac gggtgttgtt ccgatacttg
     4681 ttgaattgga tggggatgtg aatggtcata aattttccgt tagtggtgaa ggggaaggtg
     4741 acgctactta tggtaaattg acattgaaat ttatttgtac tacaggaaaa cttccagtcc
     4801 catggccgac tttggtaacg actcttacat atggagtcca atgttttagt aggtatccag
     4861 atcacatgaa acaacatgat ttctttaaat cagcaatgcc tgagggatat gttcaagaac
     4921 gaacaatatt ctttaaagat gatgggaact ataagacaag ggcagaagtc aaatttgaag
     4981 gtgatacact cgttaatcgg attgaactga aagggataga ttttaaggaa gatgggaata
     5041 ttcttgggca caaacttgaa tataattata attcccataa tgtatacata atggctgaca
     5101 agcagaagaa tggaattaaa gtcaatttta aaataaggca taatatagaa gatgggagtg
     5161 tccaattggc tgatcattac caacaaaata cacctattgg ggatggtcct gtcctcttgc
     5221 ctgataatca ttatctttct acacaatctg ctctctcaaa ggaccctaat gaaaagcgcg
     5281 accatatggt gttgttggaa tttgtcactg ctgcaggcat taccatggga atggatgaac
     5341 tctataaatg aacgcgttaa gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat
     5401 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     5461 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     5521 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     5581 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     5641 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     5701 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     5761 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     5821 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     5881 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     5941 gggccgcctc cccgcgtcga ctttaagacc aatgacttac aaggcagctg tagatcttag
     6001 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
     6061 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     6121 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     6181 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     6241 agtgtggaaa atctctagca gtacgtatag tagttcatgt catcttatta ttcagtattt
     6301 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa
     6361 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     6421 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc
     6481 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     6541 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     6601 ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata
     6661 gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     6721 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     6781 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg
     6841 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     6901 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     6961 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     7021 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     7081 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     7141 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     7201 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     7261 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
     7321 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     7381 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     7441 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     7501 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     7561 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     7621 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     7681 gcaagagcaa ctcggtcgcc gcatactcta ttctcagaat gacttggttg agtactcacc
     7741 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     7801 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     7861 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     7921 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     7981 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     8041 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     8101 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     8161 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     8221 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     8281 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     8341 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     8401 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     8461 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     8521 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     8581 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     8641 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     8701 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     8761 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     8821 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     8881 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     8941 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     9001 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     9061 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     9121 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     9181 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     9241 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     9301 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     9361 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     9421 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca
     9481 ctaaagggaa caaaagctgg agctgcaagc ttaa
//
