LOCUS       sequence-437775-       12603 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mmPing20F activation tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12603)
  AUTHORS   Johnson A, Mcassey E, Diaz S, Reagin J, Redd PS, Parrilla DR, Nguyen
            H, Stec A, McDaniel LAL, Clemente TE, Stupar RM, Parrott WA, Hancock
            CN
  TITLE     Development of mPing-based activation tags for crop insertional 
            mutagenesis.
  JOURNAL   Plant Direct. 2021 Jan 19;5(1):e00300. doi: 10.1002/pld3.300. 
            eCollection 2021 Jan.
  PUBMED    33506165
REFERENCE   2  (bases 1 to 12603)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Direct."; date: "2021-01-19"; volume: "5(1):e00300. doi"; pages: " 
            10.1002/pld3.300. eCollection 2021 Jan"
COMMENT     Sequence Label: pEarleyGate103 mmPing20F
FEATURES             Location/Qualifiers
     source          1..12603
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(111..363)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(373..924)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(930..1310)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     promoter        2306..2651
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     2594..2614
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    2663..2687
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3335..3359)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3382..4092
                     /codon_start=1
                     /gene="mgfp5"
                     /product="GFP with folding enhancement mutations"
                     /label=mgfp5
                     /note="suitable for expression in plants due to removal of 
                     a cryptic intron"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKAN
                     FKTRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(3409..3437)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     4032..4052
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             4099..4116
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      4174..4881
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(4906..4923)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4906..4922)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4915..4937)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    5125..5149
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6450..7079
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7508..8581
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8647..8841
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(9077..9099)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9185..9325
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9240..9259
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9340..9357)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9511..10099)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9591..9610)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11385..12179)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat
      121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat
      181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa
      241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc
      301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc
      361 aagattctgc agtcaaatct cggtgacggg caggaccgga cggggcggta ccggcaggct
      421 gaagtccagc tgccagaaac ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg
      481 cagcatgccg cggggggcat atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg
      541 cagcccgatg acagcgacca cgctcttgaa gccctgtgcc tccagggact tcagcaggtg
      601 ggtgtagagc gtggagccca gtcccgtccg ctggtggcgg ggggagacgt acacggtcga
      661 ctcggccgtc cagtcgtagg cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc
      721 gacctcgccg tccacctcgg cgacgagcca gggatagcgc tcccgcagac ggacgaggtc
      781 gtccgtccac tcctgcggtt cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat
      841 gtagtggttg acgatggtgc agaccgccgg catgtccgcc tcggtggcac ggcggatgtc
      901 ggccgggcgt cgttctgggc tcatcgattc gatttggtgt atcgagattg gttatgaaat
      961 tcagatgcta gtgtaatgta ttggtaattt gggaagatat aataggaagc aaggctattt
     1021 atccatttct gaaaaggcga aatggcgtca ccgcgagcgt cacgcgcatt ccgttcttgc
     1081 tgtaaagcgt tgtttggtac acttttgact agcgaggctt ggcgtgtcag cgtatctatt
     1141 caaaagtcgt taatggctgc ggatcaagaa aaagttggaa tagaaacaga atacccgcga
     1201 aattcaggcc cggttgccat gtcctacacg ccgaaataaa cgaccaaatt agtagaaaaa
     1261 taaaaactga ctcggatact tacgtcacgt cttgcgcact gatttgaaaa atctcagaat
     1321 tccaatccca caaaaatctg agcttaacag cacagttgct cctctcagag cagaatcggg
     1381 tattcaacac cctcatatca actactacgt tgtgtataac ggtccacatg ccggtatata
     1441 cgatgactgg ggttgtacaa aggcggcaac aaacggcgtt cccggagttg cacacaagaa
     1501 atttgccact attacagagg caagagcagc agctgacgcg tacacaacaa gtcagcaaac
     1561 agacaggttg aacttcatcc ccaaaggaga agctcaactc aagcccaaga gctttgctaa
     1621 ggccctaaca agcccaccaa agcaaaaagc ccactggctc acgctaggaa ccaaaaggcc
     1681 cagcagtgat ccagccccaa aagagatctc ctttgccccg gagattacaa tggacgattt
     1741 cctctatctt tacgatctag gaaggaagtt cgaaggtgaa ggtgacgaca ctatgttcac
     1801 cactgataat gagaaggtta gcctcttcaa tttcagaaag aatgctgacc cacagatggt
     1861 tagagaggcc tacgcagcag gtctcatcaa gacgatctac ccgagtaaca atctccagga
     1921 gatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga ctaattgcat
     1981 caagaacaca gagaaagaca tatttctcaa gatcagaagt actattccag tatggacgat
     2041 tcaaggcttg cttcataaac caaggcaagt aatagagatt ggagtctcta aaaaggtagt
     2101 tcctactgaa tctaaggcca tgcatggagt ctaagattca aatcgaggat ctaacagaac
     2161 tcgccgtgaa gactggcgaa cagttcatac agagtctttt acgactcaat gacaagaaga
     2221 aaatcttcgt caacatggtg gagcacgaca ctctggtcta ctccaaaaat gtcaaagata
     2281 cagtctcaga agaccaaagg gctattgaga cttttcaaca aaggataatt tcgggaaacc
     2341 tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta gaaaaggaag
     2401 gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa gatctctctg
     2461 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
     2521 ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac gtaagggatg
     2581 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
     2641 tggagaggac acgctcgaga tcacaagttt gtacaaaaaa gcaggcttaa ggccagtcac
     2701 aatgggggtt tcactggtgt gtcatgcaca tttaataggg gtaagactga ataaaaaatg
     2761 attatttgca tgaaatgggg atgagagaga aggaaagagt ttcatcctgg tgaaactcgt
     2821 cagcgtcgtt tccaagtcct cggtaacaga gcgaaacccc cgttgaggcc gattcgtttc
     2881 attcaccgga tctcttgcgt ccgcctccgc cgtgcgacct ctgcattctc aagcttcatc
     2941 agctggcttg tggggaccag acaaaaaagg aatggtgcag aattgttagg cgcacctacc
     3001 aaaagcatct ttgcctttat tgcaaagata aagcagattc ctctagtaca agtggggaac
     3061 aaaataacgt ggaaaagagc tgtcctgaca gcccactcac taatgcgtat gacgaacgca
     3121 gtgacgacca caaaagaatt ccgcgccgcg ccggattatg ggtacaaatg atcccagcaa
     3181 cttgtaccaa ttaaatgcta tgcatagtca tggaaacgtc aaagtgaaac ccctccactg
     3241 tggggattgt ttcataaaag atttcatttg aaagaagatg gtataatatt ttgggtagcc
     3301 gtgcaatgac actagccatt gtgactggcc taagacccag ctttcttgta caaagtggtg
     3361 ctcgagatgg tagatctgac tagtaaagga gaagaacttt tcactggagt tgtcccaatt
     3421 cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa
     3481 ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct
     3541 gttccgtggc caacacttgt cactactttc tcttatggtg ttcaatgctt ttcaagatac
     3601 ccagatcata tgaagcggca cgacttcttc aagagcgcca tgcctgaggg atacgtgcag
     3661 gagaggacca tcttcttcaa ggacgacggg aactacaaga cacgtgctga agtcaagttt
     3721 gagggagaca ccctcgtcaa caggatcgag cttaagggaa tcgatttcaa ggaggacgga
     3781 aacatcctcg gccacaagtt ggaatacaac tacaactccc acaacgtata catcatggcc
     3841 gacaagcaaa agaacggcat caaagccaac ttcaagaccc gccacaacat cgaagacggc
     3901 ggcgtgcaac tcgctgatca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt
     3961 ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag
     4021 agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat
     4081 gaactataca aagctagcca ccaccaccac caccacgtgt gacctaggtg agtctagaga
     4141 gttaattaag acccgggact agtccctaga gtcctgcttt aatgagatat gcgagacgcc
     4201 tatgatcgca tgatatttgc tttcaattct gttgtgcacg ttgtaaaaaa cctgagcatg
     4261 tgtagctcag atccttaccg ccggtttcgg ttcattctaa tgaatatatc acccgttact
     4321 atcgtatttt tatgaataat attctccgtt caatttactg attgtaccct actacttata
     4381 tgtacaatat taaaatgaaa acaatatatt gtgctgaata ggtttatagc gacatctatg
     4441 atagagcgcc acaataacaa acaattgcgt tttattatta caaatccaat tttaaaaaaa
     4501 gcggcagaac cggtcaaacc taaaagactg attacataaa tcttattcaa atttcaaaag
     4561 tgccccaggg gctagtatct acgacacacc gagcggcgaa ctaataacgc tcactgaagg
     4621 gaactccggt tccccgccgg cgcgcatggg tgagattcct tgaagttgag tattggccgt
     4681 ccgctctacc gaaagttacg ggcaccattc aacccggtcc agcacggcgg ccgggtaacc
     4741 gacttgctgc cccgagaatt atgcagcatt tttttggtgt atgtgggccc caaatgaagt
     4801 gcaggtcaaa ccttgacagt gacgacaaat cgttgggcgg gtccagggcg aattttgcga
     4861 caacatgtcg aggctcagca ggacctgcag gcatgcaagc ttggcactgg ccgtcgtttt
     4921 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     4981 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     5041 gcgcagcctg aatggcgaat gctagagcag cttgagcttg gatcagattg tcgtttcccg
     5101 ccttcagttt aaactatcag tgtttgacag gatatattgg cgggtaaacc taagagaaaa
     5161 gagcgtttat tagaataatc ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc
     5221 atttgtatgt gcatgccaac cacagggttc ccctcgggat caaagtactt tgatccaacc
     5281 cctccgctgc tatagtgcag tcggcttctg acgttcagtg cagccgtctt ctgaaaacga
     5341 catgtcgcac aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt cctggcgttt
     5401 tcttgtcgcg tgttttagtc gcataaagta gaatacttgc gactagaacc ggagacatta
     5461 cgccatgaac aagagcgccg ccgctggcct gctgggctat gcccgcgtca gcaccgacga
     5521 ccaggacttg accaaccaac gggccgaact gcacgcggcc ggctgcacca agctgttttc
     5581 cgagaagatc accggcacca ggcgcgaccg cccggagctg gccaggatgc ttgaccacct
     5641 acgccctggc gacgttgtga cagtgaccag gctagaccgc ctggcccgca gcacccgcga
     5701 cctactggac attgccgagc gcatccagga ggccggcgcg ggcctgcgta gcctggcaga
     5761 gccgtgggcc gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt tcgccggcat
     5821 tgccgagttc gagcgttccc taatcatcga ccgcacccgg agcgggcgcg aggccgccaa
     5881 ggcccgaggc gtgaagtttg gcccccgccc taccctcacc ccggcacaga tcgcgcacgc
     5941 ccgcgagctg atcgaccagg aaggccgcac cgtgaaagag gcggctgcac tgcttggcgt
     6001 gcatcgctcg accctgtacc gcgcacttga gcgcagcgag gaagtgacgc ccaccgaggc
     6061 caggcggcgc ggtgccttcc gtgaggacgc attgaccgag gccgacgccc tggcggccgc
     6121 cgagaatgaa cgccaagagg aacaagcatg aaaccgcacc aggacggcca ggacgaaccg
     6181 tttttcatta ccgaagagat cgaggcggag atgatcgcgg ccgggtacgt gttcgagccg
     6241 cccgcgcacg tctcaaccgt gcggctgcat gaaatcctgg ccggtttgtc tgatgccaag
     6301 ctggcggcct ggccggccag cttggccgct gaagaaaccg agcgccgccg tctaaaaagg
     6361 tgatgtgtat ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat gatgcgatga
     6421 gtaaataaac aaatacgcaa ggggaacgca tgaaggttat cgctgtactt aaccagaaag
     6481 gcgggtcagg caagacgacc atcgcaaccc atctagcccg cgccctgcaa ctcgccgggg
     6541 ccgatgttct gttagtcgat tccgatcccc agggcagtgc ccgcgattgg gcggccgtgc
     6601 gggaagatca accgctaacc gttgtcggca tcgaccgccc gacgattgac cgcgacgtga
     6661 aggccatcgg ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg gcggacttgg
     6721 ctgtgtccgc gatcaaggca gccgacttcg tgctgattcc ggtgcagcca agcccttacg
     6781 acatatgggc caccgccgac ctggtggagc tggttaagca gcgcattgag gtcacggatg
     6841 gaaggctaca agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc atcggcggtg
     6901 aggttgccga ggcgctggcc gggtacgagc tgcccattct tgagtcccgt atcacgcagc
     6961 gcgtgagcta cccaggcact gccgccgccg gcacaaccgt tcttgaatca gaacccgagg
     7021 gcgacgctgc ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa ctcatttgag
     7081 ttaatgaggt aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg gccgtccgag
     7141 cgcacgcagc agcaaggctg caacgttggc cagcctggca gacacgccag ccatgaagcg
     7201 ggtcaacttt cagttgccgg cggaggatca caccaagctg aagatgtacg cggtacgcca
     7261 aggcaagacc attaccgagc tgctatctga atacatcgcg cagctaccag agtaaatgag
     7321 caaatgaata aatgagtaga tgaattttag cggctaaagg aggcggcatg gaaaatcaag
     7381 aacaaccagg caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag
     7441 gcgtaagcgg ctgggttgcc tgccggccct gcaatggcac tggaaccccc aagcccgagg
     7501 aatcggcgtg agcggtcgca aaccatccgg cccggtacaa atcggcgcgg cgctgggtga
     7561 tgacctggtg gagaagttga aggccgcgca ggccgcccag cggcaacgca tcgaggcaga
     7621 agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag aatcccggca
     7681 accgccggca gccggtgcgc cgtcgattag gaagccgccc aagggcgacg agcaaccaga
     7741 ttttttcgtt ccgatgctct atgacgtggg cacccgcgat agtcgcagca tcatggacgt
     7801 ggccgttttc cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc gctacgagct
     7861 tccagacggg cacgtagagg tttccgcagg gccggccggc atggccagtg tgtgggatta
     7921 cgacctggta ctgatggcgg tttcccatct aaccgaatcc atgaaccgat accgggaagg
     7981 gaagggagac aagcccggcc gcgtgttccg tccacacgtt gcggacgtac tcaagttctg
     8041 ccggcgagcc gatggcggaa agcagaaaga cgacctggta gaaacctgca ttcggttaaa
     8101 caccacgcac gttgccatgc agcgtacgaa gaaggccaag aacggccgcc tggtgacggt
     8161 atccgagggt gaagccttga ttagccgcta caagatcgta aagagcgaaa ccgggcggcc
     8221 ggagtacatc gagatcgagc tagctgattg gatgtaccgc gagatcacag aaggcaagaa
     8281 cccggacgtg ctgacggttc accccgatta ctttttgatc gatcccggca tcggccgttt
     8341 tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa gccagatggt tgttcaagac
     8401 gatctacgaa cgcagtggca gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa
     8461 gctgatcggg tcaaatgacc tgccggagta cgatttgaag gaggaggcgg ggcaggctgg
     8521 cccgatccta gtcatgcgct accgcaacct gatcgagggc gaagcatccg ccggttccta
     8581 atgtacggag cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggtct
     8641 ctttcctgtg gatagcacgt acattgggaa cccaaagccg tacattggga accggaaccc
     8701 gtacattggg aacccaaagc cgtacattgg gaaccggtca cacatgtaag tgactgatat
     8761 aaaagagaaa aaaggcgatt tttccgccta aaactcttta aaacttatta aaactcttaa
     8821 aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc
     8881 gcctaccctt cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc
     8941 cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc gcggacaagc
     9001 cgcgccgtcg ccactcgacc gccggcgccc acatcaaggc accctgcctc gcgcgtttcg
     9061 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     9121 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     9181 ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc
     9241 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
     9301 cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg
     9361 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     9421 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     9481 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     9541 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     9601 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     9661 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     9721 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     9781 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     9841 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     9901 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     9961 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
    10021 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
    10081 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
    10141 gaacgaaaac tcacgttaag ggattttggt catgcattct agggaaggtg cgaacaagtc
    10201 cctgatatga gatcatgttt gtcatctgga gccatagaac agggttcatc atgagtcatc
    10261 aacttacctt cgccgacagt gaattcagca gtaagcgccg tcagaccaga aaagagattt
    10321 tcttgtcccg catggagcag attctgccat ggcaaaacat ggtggaagtc atcgagccgt
    10381 tttaccccaa ggctggtaat ggccggcgac cttatccgct ggaaaccatg ctacgcattc
    10441 actgcatgca gcattggtac aacctgagcg atggcgcgat ggaagatgct ctgtacgaaa
    10501 tcgcctccat gcgtctgttt gcccggttat ccctggatag cgccttgccg gaccgcacca
    10561 ccatcatgaa tttccgccac ctgctggagc agcatcaact ggcccgccaa ttgttcaaga
    10621 ccatcaatcg ctggctggcc gaagcaggcg tcatgatgac tcaaggcacc ttggtcgatg
    10681 ccaccatcat tgaggcaccc agctcgacca agaacaaaga gcagcaacgc gatccggaga
    10741 tgcatcagac caagaaaggc aatcagtggc actttggcat gaaggcccac attggtgtcg
    10801 atgccaagag tggcctgacc cacagcctgg tcaccaccgc ggccaacgag catgacctca
    10861 atcagctggg taatctgctg catggagagg agcaatttgt ctcagccgat gccggctacc
    10921 aaggggcgcc acagcgcgag gagctggccg aggtggatgt ggactggctg atcgccgagc
    10981 gccccggcaa ggtaagaacc ttgaaacagc atccacgcaa gaacaaaacg gccatcaaca
    11041 tcgaatacat gaaagccagc atccgggcca gggtggagca cccatttcgc atcatcaagc
    11101 gacagttcgg cttcgtgaaa gccagataca aggggttgct gaaaaacgat aaccaactgg
    11161 cgatgttatt cacgctggcc aacctgtttc gggcggacca aatgatacgt cagtgggaga
    11221 gatctcacta aaaactgggg ataacgcctt aaatggcgaa gaaacggtct aaataggctg
    11281 attcaaggca tttacgggag aaaaaatcgg ctcaaacatg aagaaatgaa atgactgagt
    11341 cagccgagaa gaatttcccc gcttattcgc accttcccta ggtactaaaa caattcatcc
    11401 agtaaaatat aatattttat tttctcccaa tcaggcttga tccccagtaa gtcaaaaaat
    11461 agctcgacat actgttcttc cccgatatcc tccctgatcg accggacgca gaaggcaatg
    11521 tcataccact tgtccgccct gccgcttctc ccaagatcaa taaagccact tactttgcca
    11581 tctttcacaa agatgttgct gtctcccagg tcgccgtggg aaaagacaag ttcctcttcg
    11641 ggcttttccg tctttaaaaa atcatacagc tcgcgcggat ctttaaatgg agtgtcttct
    11701 tcccagtttt cgcaatccac atcggccaga tcgttattca gtaagtaatc caattcggct
    11761 aagcggctgt ctaagctatt cgtataggga caatccgata tgtcgatgga gtgaaagagc
    11821 ctgatgcact ccgcatacag ctcgataatc ttttcagggc tttgttcatc ttcatactct
    11881 tccgagcaaa ggacgccatc ggcctcactc atgagcagat tgctccagcc atcatgccgt
    11941 tcaaagtgca ggacctttgg aacaggcagc tttccttcca gccatagcat catgtccttt
    12001 tcccgttcca catcataggt ggtcccttta taccggctgt ccgtcatttt taaatatagg
    12061 ttttcatttt ctcccaccag cttatatacc ttagcaggag acattccttc cgtatctttt
    12121 acgcagcggt atttttcgat cagttttttc aattccggtg atattctcat tttagccatt
    12181 tattatttcc ttcctctttt ctacagtatt taaagatacc ccaagaagct aattataaca
    12241 agacgaactc caattcactg ttccttgcat tctaaaacct taaataccag aaaacagctt
    12301 tttcaaagtt gttttcaaag ttggcgtata acatagtatc gacggagccg attttgaaac
    12361 cgcggtgatc acaggcagca acgctctgtc atcgttacaa tcaacatgct accctccgcg
    12421 agatcatccg tgtttcaaac ccggcagctt agttgccgtt cttccgaata gcatcggtaa
    12481 catgagcaaa gtctgccgcc ttacaacggc tctcccgctg acgccgtccc ggactgatgg
    12541 gctgcctgta tcgagtggtg attttgtgcc gagctgccgg tcggggagct gttggctggc
    12601 tgg
//
