LOCUS       sequence-43781-e         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Massey-Gendel E, Zhao A, Boulting G, Kim HY, Balamotis MA, Seligman 
            LM, Nakamoto RK, Bowie JU
  TITLE     Genetic selection system for improving recombinant membrane protein 
            expression in E. coli.
  JOURNAL   Protein Sci. 2009 Feb . 18(2):372-83.
  PUBMED    19165721
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2009 Feb . 18(2):372-83."
COMMENT     Sequence Label: pCURE
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             48..70
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 gggctagcga attcgagctc ggtacccggg gatcctctag aaataatttt gtttaacttt
       61 aagaaggaga tatatatatg aatacaaaat ataataaaga gttcttactc tacttagcag
      121 ggtttgtaga cggtgacggt agcataatcg ctcaaattaa gcctaatcag tcttataaat
      181 ttaagcatca gctatcactc acgttccaag tcacgcaaaa gacacagaga cgttggtttt
      241 taggcaaatt agtggatgaa attggggttg gttatgtaag agataggggt agcgtttcgg
      301 attatattct aagcgaaatc aagcctttgc ataatttttt aacacaacta caaccttttc
      361 taaaactaaa acaaaaacaa gcaaatttag ttttaaaaat tattgaacaa cttccgtcag
      421 caaaagaatc cccggacaaa ttcttagaag tttgtacatg ggcggatcaa attgcagctc
      481 tgaatgattc gaagacgcgt aaaacaactt ctgaaaccgt tcgtgctgtg ctagacagtt
      541 taagtgaaaa aaagaaatcg tccccgtaac agctgttcta ttaggtgtta catgctgttc
      601 atctgttaca ttgtcgatct gttcatggtg aacagctttg aatgcaccaa aaactcgtaa
      661 aagctctgat gtatctatct tttttacacc gttttcatct gtgcatatgg acagttttcc
      721 ctttgatatg taacggtgaa cagttgttct acttttgttt gttagtcttg atgcttcact
      781 gatagataca agagccataa gaacctcaga tccttccgta tttagccagt atgttctcta
      841 gtgtggtcgt gt
//
