LOCUS       sequence-437813-       11120 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAVS1-targeting vector designed for the iPS2-seq platform to express
            a single-copy, tetracycline-inducible shRNA in human pluripotent 
            stem cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11120)
  AUTHORS   Balmas E, Ratto ML, Snijders KE, Becca S, Liaci C, Ricca I, Merlo 
            GR, Calogero RA, Alessandri L, Mendjan S, Bertero A
  TITLE     Single Cell Transcriptional Perturbome in Pluripotent Stem Cell 
            Models
  JOURNAL   Mol Syst Biol (2025). https://doi.org/10.1038/s44320-025-00172-8
REFERENCE   2  (bases 1 to 11120)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol (2025). https://doi.org/10.1038/s44320-025-00172-8"
COMMENT     Sequence Label: pAAV-Puro_siKD2.0
FEATURES             Location/Qualifiers
     source          1..11120
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        242..260
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    285..406
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(413..1408)
                     /codon_start=1
                     /product="truncated herpes simplex virus thymidine kinase 
                     (Salomon et al., 1995)"
                     /label=Delta-TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRV
                     LGASETIANIYTTQHRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGS
                     SHAPPPALTLIFDRHPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGAL
                     PEDRHIDRLAKRQRPGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGTAV
                     PPQGAEPQSNAGPRPHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFI
                     LDYDQSPAGCRDALLQLTSGMIQTHVTTPGSIPTICDLARTFAREMGEAN"
     promoter        complement(1439..1486)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(1498..1997)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1546..1565)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     1939..1962
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     misc_feature    2026..2829
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    2836..2861
                     /label=SA
                     /note="splice acceptor site"
     CDS             2885..2938
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2948..3547
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2948..2967)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3444..3464
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    3585..3719
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     3863..3882
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    3928..3946
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(3958..3974)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3959..3975)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     enhancer        4037..4340
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          4620..5636
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     5560..5582
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     5644..5663
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             5707..5727
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(6375..6392)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6381..6492
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    6622..7458
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(7492..7511)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7493..7511)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7518..7535)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7518..7534)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7527..7549)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             7672..7974
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     7853..7872
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             8323..9117
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8323..9117
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8377..8396)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8987..9006
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             complement(join(9367..10158,10159..10227))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9990..10009
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10351..10939
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10840..10859
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11093..11110
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctca
      241 gaattaaccc tcactaaagg gactagtcct gcaggcagac atgataagat acattgatga
      301 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
      361 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttctta agtcagttag
      421 cctcccccat ctcccgggca aacgtgcgcg ccaggtcgca gatcgtcggt atggagcctg
      481 gggtggtgac gtgggtctgg atcatcccgg aggtaagttg cagcagggcg tcccggcagc
      541 cggcgggcga ttggtcgtaa tccaggataa agacgtgcat gggacggagg cgtttggcca
      601 agacgtccaa ggcccaggca aacacgttgt acaggtcgcc gttgggggcc agcaactcgg
      661 gggcccgaaa cagggtaaat aacgtgtccc cgatatgggg tcgtgggccc gcgttgctct
      721 ggggctcggc accctggggc ggcacggccg tccccgaaag ctgtccccaa tcctcccgcc
      781 acgacccgcc gccctgcaga taccgcaccg tattggcaag cagcccgtaa acgcggcgaa
      841 tcgcggccag catagccagg tcaagccgct cgccggggcg ctggcgtttg gccaggcggt
      901 cgatgtgtct gtcctccgga agggccccca acacgatgtt tgtgccgggc aaggtcggcg
      961 ggatgagggc cacgaacgcc agcacggcct ggggggtcat gctgcccata aggtatcgcg
     1021 cggccgggta gcacaggagg gcggcgatgg gatggcggtc gaagatgagg gtgagggccg
     1081 ggggcggggc atgtgagctc ccagcctccc ccccgatatg aggagccaga acggcgtcgg
     1141 tcacggcata aggcatgccc attgttatct gggcgcttgt cattaccacc gccgcgtccc
     1201 cggccgatat ctcaccctgg tcaaggcggt gttgtgtggt gtagatgttc gcgattgtct
     1261 cggaagcccc cagcacccgc cagtaagtca tcggctcggg tacgtagacg atatcgtcgc
     1321 gcgaacccag ggccaccagc agttgcgtgg tggtggtttt ccccatcccg tggggaccgt
     1381 ctatataaac ccgcagtagc gtgggcatgg tggccgatct ggtttagttc ctcaccttgt
     1441 cgtattatac tatgccgata tactatgccg atgattaatt gtcaacaggc tgcaggtcga
     1501 aaggcccgga gatgaggaag aggagaacag cgcggcagac gtgcgctttt gaagcgtgca
     1561 gaatgccggg cctccggagg accttcgggc gcccgccccg cccctgagcc cgcccctgag
     1621 cccgcccccg gacccacccc ttcccagcct ctgagcccag aaagcgaagg agcaaagctg
     1681 ctattggccg ctgccccaaa ggcctacccg cttccattgc tcagcggtgc tgtccatctg
     1741 cacgagacta gtgagacgtg ctacttccat ttgtcacgtc ctgcacgacg cgagctgcgg
     1801 ggcggggggg aacttcctga ctaggggagg agtagaaggt ggcgcgaagg ggccaccaaa
     1861 gaacggagcc ggttggcgcc taccggtgga tgtggaatgt gtgcgaggcc agaggccact
     1921 tgtgtagcgc caagtgccca gcggggctgc taaagcgcat gctccagact gccttgggaa
     1981 aagcgcctcc cctacccggt agaattttta aacgaattcg ccctttgctt tctctgacca
     2041 gcattctctc ccctgggcct gtgccgcttt ctgtctgcag cttgtggcct gggtcacctc
     2101 tacggctggc ccagatcctt ccctgccgcc tccttcaggt tccgtcttcc tccactccct
     2161 cttccccttg ctctctgctg tgttgctgcc caaggatgct ctttccggag cacttccttc
     2221 tcggcgctgc accacgtgat gtcctctgag cggatcctcc ccgtgtctgg gtcctctccg
     2281 ggcatctctc ctccctcacc caaccccatg ccgtcttcac tcgctgggtt cccttttcct
     2341 tctccttctg gggcctgtgc catctctcgt ttcttaggat ggccttctcc gacggatgtc
     2401 tcccttgcgt cccgcctccc cttcttgtag gcctgcatca tcaccgtttt tctggacaac
     2461 cccaaagtac cccgtctccc tggctttagc cacctctcca tcctcttgct ttctttgcct
     2521 ggacaccccg ttctcctgtg gattcgggtc acctctcact cctttcattt gggcagctcc
     2581 cctacccccc ttacctctct agtctgtgct agctcttcca gccccctgtc atggcatctt
     2641 ccaggggtcc gagagctcag ctagtcttct tcctccaacc cgggccccta tgtccacttc
     2701 aggacagcat gtttgctgcc tccagggatc ctgtgtcccc gagctgggac caccttatat
     2761 tcccagggcc ggttaatgtg gctctggttc tgggtacttt tatctgtccc ctccacccca
     2821 cagtggggca agcttctgac ctcttctctt cctcccacag ggcctcgaga gttctggcag
     2881 cggagagggc agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggccctag
     2941 gctcgagatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag
     3001 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
     3061 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
     3121 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac
     3181 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
     3241 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
     3301 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
     3361 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
     3421 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
     3481 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
     3541 tgcctgatct agagggcccg tttaaacccg ctgatcagcc tcgaaacttg tttattgcag
     3601 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     3661 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcg
     3721 tcaattcgaa cgctgacgtc atcaacccgc tccaaggaat cgcgggccca gtgtcactag
     3781 gcgggaacac ccagcgcgcg tgcgccctgg caggaagatg gctgtgaggg acaggggagt
     3841 ggcgccctgc aatatttgca tgtcgctatg tgttctggga aatcaccata aacgtgaaat
     3901 gtctttggat ttgggaatct tataagttcc ctatcagtga tagagatcta agcttatcga
     3961 taccgtcgac ctcgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
     4021 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     4081 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     4141 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     4201 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     4261 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
     4321 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
     4381 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
     4441 tgggggcggg gggggggggg gggcgcgcgc cggggggggg gggggggggs ggggggsggg
     4501 gsggggsgrg gsggrgrggk gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc
     4561 cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg
     4621 gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc
     4681 cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc
     4741 cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa
     4801 gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg
     4861 tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct
     4921 gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg
     4981 gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt
     5041 gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc
     5101 cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt acggggcgtg
     5161 gcgcggggct cgccgtgccg ggcggggggt ggcggcaggk gggggtgccg ggcggggcgg
     5221 ggccgcckcg ggccggggrg ggctcggggg rggggcgcgg cggcccccgg agcgccggcg
     5281 gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc
     5341 agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc
     5401 cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg
     5461 gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc
     5521 ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga
     5581 ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc
     5641 tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaatta attcggatcc
     5701 accatgccca agaaaaagcg gaaggtgtcc cggctggaca agagcaaagt gatcaacagc
     5761 gccctggaac tgctgaacga agtgggcatc gagggcctga ccacccggaa gctggcccag
     5821 aaactgggcg tggaacagcc caccctgtac tggcacgtga agaacaagcg ggccctgctg
     5881 gacgccctgg ccatcgagat gctggaccgg caccacacac acttttgccc cctggaaggc
     5941 gaaagctggc aggacttcct gcggaacaac gccaagagct tcagatgcgc cctgctgagc
     6001 caccgggacg gcgccaaagt gcacctgggc accagaccca ccgagaagca gtacgagaca
     6061 ctggaaaacc agctggcctt cctgtgccag cagggcttca gcctggaaaa cgccctgtac
     6121 gccctgagcg ccgtgggcca ctttaccctg ggctgcgtgc tggaagatca ggaacaccag
     6181 gtcgccaaag aggaaagaga gacacccacc accgacagca tgccccccct gctgagacag
     6241 gccatcgagc tgttcgatca tcaaggcgcc gagcccgcct tcctgttcgg cctggaactg
     6301 atcatctgcg gcctcgagaa gcagctgaag tgcgagagcg gctcccccag atgaacgcgt
     6361 agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     6421 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     6481 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     6541 ggacagcaag ggggaggatt gggaagagaa tagcaggcat gctgggggtc tcgagggggg
     6601 gcccggacag tactaagctt tactagggac aggattggtg acagaaaagc cccatcctta
     6661 ggcctcctcc ttcctagtct cctgatattg ggtctaaccc ccacctcctg ttaggcagat
     6721 tccttatctg gtgacacacc cccatttcct ggagccatct ctctccttgc cagaacctct
     6781 aaggtttgct tacgatggag ccagagagga tcctgggagg gagagcttgg cagggggtgg
     6841 gagggaaggg ggggatgcgt gacctgcccg gttctcagtg gccaccctgc gctaccctct
     6901 cccagaacct gagctgctct gacgcggctg tctggtgcgt ttcactgatc ctggtgctgc
     6961 agcttcctta cacttcccaa gaggagaagc agtttggaaa aacaaaatca gaataagttg
     7021 gtcctgagtt ctaactttgg ctcttcacct ttctagtccc caatttatat tgttcctccg
     7081 tgcgtcagtt ttacctgtga gataaggcca gtagccagcc ccgtcctggc agggctgtgg
     7141 tgaggagggg ggtgtccgtg tggaaaactc cctttgtgag aatggtgcgt cctaggtgtt
     7201 caccaggtcg tggccgcctc tactcccttt ctctttctcc atccttcttt ccttaaagag
     7261 tccccagtgc tatctgggac atattcctcc gcccagagca gggtcccgct tccctaaggc
     7321 cctgctctgg gcttctgggt ttgagtcctt ggcaagccca ggagaggcgc tcaggcttcc
     7381 ctgtccccct tcctcgtcca ccatctcatg cccctggctc tcctgcccct tccctacagg
     7441 ggttcctggc tctgctctaa gggcgaattc gcggccgcta aattcaattc gccctatagt
     7501 gagtcgtatt acaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     7561 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     7621 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tatacgtacg gcagtttaag
     7681 gtttacacct ataaaagaga gagccgttat cgtctgtttg tggatgtaca gagtgatatt
     7741 attgacacgc cggggcgacg gatggtgatc cccctggcca gtgcacgtct gctgtcagat
     7801 aaagtctccc gtgaacttta cccggtggtg catatcgggg atgaaagctg gcgcatgatg
     7861 accaccgata tggccagtgt gccggtctcc gttatcgggg aagaagtggc tgatctcagc
     7921 caccgcgaaa atgacatcaa aaacgccatt aacctgatgt tctggggaat ataaatgtca
     7981 ggcatgagat tatcaaaaag gatcttcacc tagatccttt tcacgtagaa agccagtccg
     8041 cagaaacggt gctgaccccg gatgaatgtc agctactggg ctatctggac aagggaaaac
     8101 gcaagcgcaa agagaaagca ggtagcttgc agtgggctta catggcgata gctagactgg
     8161 gcggttttat ggacagcaag cgaaccggaa ttgccagctg gggcgccctc tggtaaggtt
     8221 gggaagccct gcaaagtaaa ctggatggct ttcttgccgc caaggatctg atggcgcagg
     8281 ggatcaagct ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga
     8341 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa
     8401 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt
     8461 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg
     8521 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa
     8581 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac
     8641 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt
     8701 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact
     8761 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg
     8821 ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg
     8881 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc
     8941 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt
     9001 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc
     9061 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgaatt
     9121 attaacgctt acaatttcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     9181 caccgcatca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     9241 ctaaatacat tcaaatatgt atccgctcat gagattatca aaaaggatct tcacctagat
     9301 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     9361 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     9421 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     9481 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     9541 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     9601 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     9661 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     9721 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     9781 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     9841 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     9901 cttttctgtg actggtgaat actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     9961 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
    10021 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
    10081 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
    10141 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
    10201 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
    10261 tcagggttat tgtctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
    10321 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
    10381 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
    10441 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
    10501 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
    10561 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
    10621 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
    10681 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
    10741 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
    10801 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
    10861 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
    10921 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
    10981 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
    11041 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
    11101 agtgagcgag gaagcggaag
//
