LOCUS       sequence-437819-        8915 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses wtCas9-NG with double processed direct repeats (15nt) and 
            a non-target spacer in between at 3' end, mCherry tag, and the gRNA 
            targeting HEK293T-site3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8915)
  TITLE     CRISPR-Cas transgene repressible element
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 1203U-U6-gRNA-pCMV-wtCas9-NG-P2A-mcherry-3'dmDR
FEATURES             Location/Qualifiers
     source          1..8915
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    1062..1286
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     1275..1292
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(1315..1332)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(1393..1413)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(1426..2133)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1701..1720)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1970..1988
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(2134..2190)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(2200..2220)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(2263..6363)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SIRPKRNSDKLIARKKDWDPKKYGGFVSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASARFLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPRAFKYFDTTIDRKVYRSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(6364..6384)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(6433..6452)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6434..6452)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(6494..6697)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(6523..6543)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        6698..7077
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_RNA        complement(7117..7192)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(7213..7461)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(7271..7290)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(7441..7461)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     7682..7701
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        7780..7884
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7885..7953,7954..8745)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8103..8122)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccctag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt
     1021 cgacggtatc gagccccagc tggttctttc cgcctcagaa gccatagagc ccaccgcatc
     1081 cccagcatgc ctgctattgt cttcccaatc ctcccccttg ctgtcctgcc ccaccccacc
     1141 ccccagaata gaatgacacc tactcagaca atgcgatgca atttcctcat tttattagga
     1201 aaggacagtg ggagtggcac cttccagggt caaggaaggc acgggggagg ggcaaacaac
     1261 agatggctgg caactagaag gcacagtcga ggctgatcag cgggtttaaa ctcaatggtg
     1321 atggtgatga tggttccgtg acatcaaaaa tacaagggct accaggtagg ttccgtgaca
     1381 tcaaaccggt tagactttcc tcttcttctt gggagaacca ccagacttgt acagctcgtc
     1441 catgccgccg gtggagtggc ggccctcggc gcgttcgtac tgttccacga tggtgtagtc
     1501 ctcgttgtgg gaggtgatgt ccaacttgat gttgacgttg taggcgccgg gcagctgcac
     1561 gggcttcttg gccttgtagg tggtcttgac ctcagcgtcg tagtggccgc cgtccttcag
     1621 cttcagcctc tgcttgatct cgcccttcag ggcgccgtcc tcggggtaca tccgctcgga
     1681 ggaggcctcc cagcccatgg tcttcttctg cattacgggg ccgtcggagg ggaagttggt
     1741 gccgcgcagc ttcaccttgt agatgaactc gccgtcctgc agggaggagt cctgggtcac
     1801 ggtcaccacg ccgccgtcct cgaagttcat cacgcgctcc cacttgaagc cctcggggaa
     1861 ggacagcttc aagtagtcgg ggatgtcggc ggggtgcttc acgtaggcct tggagccgta
     1921 catgaactga ggggacagga tgtcccaggc gaagggcagg gggccaccct tggtcacctt
     1981 cagcttggcg gtctgggtgc cctcgtaggg gcggccctcg ccctcgccct cgatctcgaa
     2041 ctcgtggccg ttcacggagc cctccatgtg caccttgaag cgcatgaact ccttgatgat
     2101 ggccatgtta tcctcctcgc ccttgctcac cataggtcca gggttctcct ccacgtctcc
     2161 agcctgcttc agcaggctga agttagtagc tccgcttccg actttcctct tcttcttggg
     2221 ctcgaattcg ctgccgtcgg cggttctttt tgagccgcca gagtcacctc ccagctgaga
     2281 caggtcgatc cgtgtctcgt acaggccggt gatgctctgg tggatcaggg tggcgtccag
     2341 cacctctttg gtgctacggt acaccttccg gtcgatggtg gtgtcaaagt acttgaaggc
     2401 cctaggggct cccagattgg tcagggtaaa caggtggatg atattctcgg cctgctctct
     2461 gatgggctta tcccggtgct tgttgtaggc ggacagcact ttgtccagat tagcgtcggc
     2521 caggatcact ctcttggaga actcgctgat ctgctcgatg atctcgtcca ggtagtgctt
     2581 gtgctgttcc acaaacagct gtttctgctc attatcctcg ggggagccct tcagcttctc
     2641 atagtggctg gccaggtaca ggaagttcac atatttggag ggcagggcca gttcgtttcc
     2701 cttctgcagg aaccgggcag aggccagcat tctcttccgg ccgttttcca gctcgaacag
     2761 ggagtactta ggcagcttga tgatcaggtc ctttttcact tctttgtagc ccttggcttc
     2821 cagaaagtcg atgggattct tctcgaagct gcttctttcc atgatggtga tccccagcag
     2881 ctctttcaca ctcttcagtt tcttggactt gcccttttcc actttggcca ccaccagcac
     2941 agaataggcc acggtggggc tcacgaagcc gccgtacttc ttagggtccc agtccttctt
     3001 tctggcgatc agcttatcgc tgttcctctt gggacggata gactctttgc tgaagccgcc
     3061 tgtctgcacc tcggtctttt tcacgatatt cacttggggc atgctcagca ctttccgcac
     3121 ggtggcaaaa tcccggccct tatcccacac gatctccccg gtttcgccgt ttgtctcgat
     3181 cagaggccgc ttccggatct cgccgttggc cagggtaatc tcggtcttga aaaagttcat
     3241 gatgttgctg tagaagaagt acttggcggt agccttgccg atttcctgct cgctcttggc
     3301 gatcatcttc cgcacgtcgt acaccttgta gtcgccgtac acgaactcgc tttccagctt
     3361 agggtacttt ttgatcaggg cggttcccac gacggcgttc aggtaggcgt cgtgggcgtg
     3421 gtggtagttg ttgatctcgc gcactttgta aaactggaaa tccttccgga aatcggacac
     3481 cagcttggac ttcagggtga tcactttcac ttcccggatc agcttgtcat tctcgtcgta
     3541 cttagtgttc atccgggagt ccaggatctg tgccacgtgc tttgtgatct gccgggtttc
     3601 caccagctgt ctcttgatga agccggcctt atccagttcg ctcaggccgc ctctctcggc
     3661 cttggtcaga ttgtcgaact ttctctgggt aatcagcttg gcgttcagca gctgccgcca
     3721 gtagttcttc atcttcttca cgacctcttc ggagggcacg ttgtcgctct tgccccggtt
     3781 cttgtcgctt ctggtcagca ccttgttgtc gatggagtcg tccttcagaa agctctgagg
     3841 cacgatatgg tccacatcgt agtcggacag ccggttgatg tccagttcct ggtccacgta
     3901 catatcccgc ccattctgca ggtagtacag gtacagcttc tcgttctgca gctgggtgtt
     3961 ttccacgggg tgttctttca ggatctggct gcccagctct ttgatgccct cttcgatccg
     4021 cttcattctc tcgcggctgt tcttctgtcc cttctgggtg gtctggttct ctctggccat
     4081 ttcgatcacg atgttctcgg gcttgtgccg gcccatcact ttcacgagct cgtccaccac
     4141 cttcactgtc tgcaggatgc ccttcttaat ggcggggctg ccggccagat tggcaatgtg
     4201 ctcgtgcagg ctatcgccct ggccggacac ctgggctttc tggatgtcct ctttaaaggt
     4261 caggctgtcg tcgtggatca gctgcatgaa gtttctgttg gcgaagccgt cggacttcag
     4321 gaaatccagg attgtcttgc cggactgctt gtcccggatg ccgttgatca gcttccggct
     4381 cagcctgccc cagccggtgt atctccgccg cttcagctgc ttcatcactt tgtcgtcgaa
     4441 caggtgggca taggttttca gccgttcctc gatcatctct ctgtcctcaa acagtgtcag
     4501 ggtcagcacg atatcttcca gaatgtcctc gttttcctca ttgtccagga agtccttgtc
     4561 cttgataatt ttcagcagat cgtggtatgt gcccagggag gcgttgaacc gatcttccac
     4621 gccggagatt tccacggagt cgaagcactc gattttcttg aagtagtcct ctttcagctg
     4681 cttcacggtc actttccggt tggtcttgaa cagcaggtcc acgatggcct ttttctgctc
     4741 gccgctcagg aaggcgggct ttctcattcc ctcggtcacg tatttcactt tggtcagctc
     4801 gttatacacg gtgaagtact cgtacagcag gctgtgcttg ggcagcacct tctcgttggg
     4861 caggttctta tcgaagttgg tcatccgctc gatgaagctc tgggcggaag cgcccttgtc
     4921 caccacttcc tcgaagttcc agggggtgat ggtttcctcg ctctttctgg tcatccaggc
     4981 gaatctgctg tttcccctgg ccagagggcc cacgtagtag gggatgcgga aggtcaggat
     5041 cttctcgatc ttttcccggt tgtccttcag gaatgggtaa aaatcttcct gccgccgcag
     5101 aatggcgtgc agctctccca ggtggatctg gtgggggatg ctgccgttgt cgaaggtccg
     5161 ctgcttccgc agcaggtcct ctctgttcag cttcacgagc agttcctcgg tgccgtccat
     5221 cttttccagg atgggcttga tgaacttgta gaactcttcc tggctggctc cgccgtcaat
     5281 gtagccggcg tagccgttct tgctctggtc gaagaaaatc tctttgtact tctcaggcag
     5341 ctgctgccgc acgagagctt tcagcagggt caggtcctgg tggtgctcgt cgtatctctt
     5401 gatcatagag gcgctcaggg gggccttggt gatctcggtg ttcactctca ggatgtcgct
     5461 cagcaggatg gcgtcggaca ggttcttggc ggccagaaac aggtcggcgt actggtcgcc
     5521 gatctgggcc agcaggttgt ccaggtcgtc gtcgtaggtg tccttgctca gctgcagttt
     5581 ggcatcctcg gccaggtcga agttgctctt gaagttgggg gtcaggccca ggctcagggc
     5641 aatcaggttt ccgaacaggc cattcttctt ctcgccgggc agctgggcga tcagattttc
     5701 cagccgtctg ctcttgctca gtctggcaga caggatggcc ttggcgtcca cgccgctggc
     5761 gttgatgggg ttttcctcga acagctggtt gtaggtctgc accagctgga tgaacagctt
     5821 gtccacgtcg ctgttgtcgg ggttcaggtc gccctcgatc aggaagtggc cccggaactt
     5881 gatcatgtgg gccagggcca gatagatcag ccgcaggtcg gccttgtcgg tgctgtccac
     5941 cagtttcttt ctcaggtggt agatggtggg gtacttctcg tggtaggcca cctcgtccac
     6001 gatgttgccg aagatggggt gccgctcgtg cttcttatcc tcttccacca ggaaggactc
     6061 ttccagtctg tggaagaagc tgtcgtccac cttggccatc tcgttgctga agatctcttg
     6121 cagatagcag atccggttct tccgtctggt gtatcttctt ctggcggttc tcttcagccg
     6181 ggtggcctcg gctgtttcgc cgctgtcgaa cagcagggct ccgatcaggt tcttcttgat
     6241 gctgtgccgg tcggtgttgc ccagcacctt gaatttcttg ctgggcacct tgtactcgtc
     6301 ggtgatcacg gcccagccca cagagttggt gccgatatcc aggccgatgc tgtacttctt
     6361 gtcgactttc cgcttcttct ttggtgactc gaactcgctt ccgtcggctg tccgtttcat
     6421 ggtggcggct ctccctatag tgagtcgtat tagcggccgc ggatctctag cggatctgac
     6481 ggttcactaa accagctctg cttatataga cctcccaccg tacacgccta ccgcccattt
     6541 gcgtcaatgg ggcggagttg ttacgacatt ttggaaagtc ccgttgattt tggtgccaaa
     6601 acaaactccc attgacgtca atggggtgga gacttggaaa tccccgtgag tcaaaccgct
     6661 atccacgccc attgatgtac tgccaaaacc gcatcaccat ggtaatagcg atgactaata
     6721 cgtagatgta ctgccaagta ggaaagtccc ataaggtcat gtactgggca taatgccagg
     6781 cgggccattt accgtcattg acgtcaatag ggggcgtact tggcatatga tacacttgat
     6841 gtactgccaa gtgggcagtt taccgtaaat actccaccca ttgacgtcaa tggaaagtcc
     6901 ctattggcgt tactatggga acatacgtca ttattgacgt caatgggcgg gggtcgttgg
     6961 gcggtcagcc aggcgggcca tttaccgtaa gttatgtaac gcggaactcc atatatgggc
     7021 tatgaactaa tgaccccgta attgattact attaataact agtcaataat caatgtcaac
     7081 gcgtatatct ggcccgtaca tcgaagctta aaaaaagcac cgactcggtg ccactttttc
     7141 aagttgataa cggactagcc ttatttaaac ttgctatttc tagctcttaa actcacgtgc
     7201 tcagtctggg ccggtgtttc gtcctttcca caagatatat aaagccaaga aatcgaaata
     7261 ctttcaagtt acggtaagca tatgatagtc cattttaaaa cataatttta aaactgcaaa
     7321 ctacccaaga aattattact ttctacgtca cgtattttgt actaatatct ttgtgtttac
     7381 agtcaaatta attctaatta tctctctaac agccttgtat cgtatatgca aatatgaagg
     7441 aatcatggga aataggccct cttcctgccc gaccttggta ccggatccag tcgactgaat
     7501 tggttccttt aaagcctgct tttttgtaca gctagccgaa gcagcgcaaa acgcctaacc
     7561 ctaagcagat tcttcatgca attgtcggtc aagccttgcc ttgttgtagc ttaaattttg
     7621 ctcgcgcact actcagcgac ctccaacaca caagcaggga gcagatactg gcttaactat
     7681 gcggcatcag agcagattgt actgagagtc gaccctaggg gatcgggaga tcgatctccc
     7741 gatccgtcga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     7801 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     7861 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     7921 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     7981 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     8041 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     8101 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     8161 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     8221 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     8281 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     8341 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     8401 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     8461 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     8521 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     8581 tctggagccg gtgagcgtgg atctcgcggt atcattgcag cactggggcc agatggtaag
     8641 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     8701 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     8761 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     8821 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     8881 gtgtcagacc ccgtagaaaa gatcaaagga tcttc
//
