LOCUS       sequence-437842-        9442 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  NKX2-1-GFP fusion protein lentiviral overexpression using an SFFV 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9442)
  AUTHORS   Pulice JL, Meyerson M
  TITLE     Dosage amplification dictates oncogenic regulation by the NKX2-1 
            lineage factor in lung adenocarcinoma.
  JOURNAL   bioRxiv [Preprint]. 2023 Oct 27:2023.10.26.563996. doi: 
            10.1101/2023.10.26.563996.
  PUBMED    37994369
REFERENCE   2  (bases 1 to 9442)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2023 Oct 27:2023.10.26.563996. doi: 
            10.1101/2023.10.26.563996."
COMMENT     Sequence Label: pLX-SFFV_NKX2-1_Short_V5-GFP
FEATURES             Location/Qualifiers
     source          1..9442
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1766..1976
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1788..1984
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1835..1970
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2016..2618
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2016..2035)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2512..2532
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2661..2778
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2847..3253
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     protein_bind    3285..3309
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4426..4450)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4458..4499
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5295..5883
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5348..5368)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5766..5777)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5955..6188
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6266..6387
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6303..6322)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6357..6376
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6427..6562
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6582..6601)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6583..6601)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6611..6628)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6611..6627)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6620..6642)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6769..7224
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6856..6875)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7066..7087
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7250..7354
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7355..7423,7424..8215)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRILYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7573..7592)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8386..8974
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8875..8894
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9128..9145
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9262..9283
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9298..9303,9304..9321,9322..9328)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9336..9352
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9341..9363
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9360..9376
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9360..9376
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9395..9415
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9397..9415
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggaccca tgcattgcat ctcaattagt cagcaaccag
     1741 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc gtctcaatta
     1801 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     1861 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     1921 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     1981 caaaaagctt tctagaggta ccaccggagc ttaccatgac cgagtacaag cccacggtgc
     2041 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg
     2101 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc
     2161 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
     2221 acggcgccgc cgtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
     2281 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
     2341 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
     2401 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
     2461 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
     2521 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
     2581 gcacctggtg catgacccgc aagcccggtg ccagttaacg aattctagat cttgagacaa
     2641 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg
     2701 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     2761 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccact ttggcgccgg
     2821 ctcgaggcct gcaggagata gctgcagtaa cgccattttg caaggcatgg aaaaatacca
     2881 aaccaagaat agagaagttc agatcaaggg cgggtacatg aaaatagcta acgttgggcc
     2941 aaacaggata tctgcggtga gcagtttcgg ccccggcccg gggccaagaa cagatggtca
     3001 ccgcagtttc ggccccggcc cgaggccaag aacagatggt ccccagatat ggcccaaccc
     3061 tcagcagttt cttaagaccc atcagatgtt tccaggctcc cccaaggacc tgaaatgacc
     3121 ctgcgcctta tttgaattaa ccaatcagcc tgcttctcgc ttctgttcgc gcgcttctgc
     3181 ttcccgagct ctataaaaga gctcacaacc cctcactcgg cgcgccagtc ctccgacaga
     3241 ctgagtcggc cggtcgaatc aagcttgcta gcatcgattg atcaacaagt ttgtacaaaa
     3301 aagttggcac catgtcgatg agtccaaagc acacgactcc gttctcagtg tctgacatct
     3361 tgagtcccct ggaggaaagc tacaagaaag tgggcatgga gggcggcggc ctcggggctc
     3421 cgctggcggc gtacaggcag ggccaggcgg caccgccaac agcggccatg cagcagcacg
     3481 ccgtggggca ccacggcgcc gtcaccgccg cctaccacat gacggcggcg ggggtgcccc
     3541 agctctcgca ctccgccgtg gggggctact gcaacggcaa cctgggcaac atgagcgagc
     3601 tgccgccgta ccaggacacc atgaggaaca gcgcctctgg ccccggatgg tacggcgcca
     3661 acccagaccc gcgcttcccc gccatctccc gcttcatggg cccggcgagc ggcatgaaca
     3721 tgagcggcat gggcggcctg ggctcgctgg gggacgtgag caagaacatg gccccgctgc
     3781 caagcgcgcc gcgcaggaag cgccgggtgc tcttctcgca ggcgcaggtg tacgagctgg
     3841 agcgacgctt caagcaacag aagtacctgt cggcgccgga gcgcgagcac ctggccagca
     3901 tgatccacct gacgcccacg caggtcaaga tctggttcca gaaccaccgc tacaaaatga
     3961 agcgccaggc caaggacaag gcggcgcagc agcaactgca gcaggacagc ggcggcggcg
     4021 ggggcggcgg gggcaccggg tgcccgcagc agcaacaggc tcagcagcag tcgccgcgac
     4081 gcgtggcggt gccggtcctg gtgaaagacg gcaaaccgtg ccaggcgggt gcccccgcgc
     4141 cgggcgccgc cagcctacaa ggccacgcgc agcagcaggc gcagcaccag gcgcaggccg
     4201 cgcaggcggc ggcagcggcc atctccgtgg gcagcggtgg cgccggcctt ggcgcacacc
     4261 cgggccacca gccaggcagc gcaggccagt ctccggacct ggcgcaccac gccgccagcc
     4321 ccgcggcgct gcagggccag gtatccagcc tgtcccacct gaactcctcg ggctcggact
     4381 acggcaccat gtcctgctcc accttgctat acggtcggac ctggttgcca actttcttgt
     4441 acaaagtggt taccggtggt aagcctatcc ctaaccctct cctcggtctc gattctacga
     4501 cgcgtgctga agctgctgca aaggaagctg cagctaagga ggctgcagct aaggctggat
     4561 ccgtcagtaa aggggaagaa ttgtttacgg gtgttgttcc gatacttgtt gaattggatg
     4621 gggatgtgaa tggtcataaa ttttccgtta gtggtgaagg ggaaggtgac gctacttatg
     4681 gtaaattgac attgaaattt atttgtacta caggaaaact tccagtccca tggccgactt
     4741 tggtaacgac tcttacatat ggagtccaat gttttagtag gtatccagat cacatgaaac
     4801 aacatgattt ctttaaatca gcaatgcctg agggatatgt tcaagaacga acaatattct
     4861 ttaaagatga tgggaactat aagacaaggg cagaagtcaa atttgaaggt gatacactcg
     4921 ttaatcggat tgaactgaaa gggatagatt ttaaggaaga tgggaatatt cttgggcaca
     4981 aacttgaata taattataat tcccataatg tatacataat ggctgacaag cagaagaatg
     5041 gaattaaagt caattttaaa ataaggcata atatagaaga tgggagtgtc caattggctg
     5101 atcattacca acaaaataca cctattgggg atggtcctgt cctcttgcct gataatcatt
     5161 atctttctac acaatctgct ctctcaaagg accctaatga aaagcgcgac catatggtgt
     5221 tgttggaatt tgtcactgct gcaggcatta ccatgggaat ggatgaactc tataaatgaa
     5281 cgcgttaagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     5341 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     5401 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     5461 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     5521 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     5581 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     5641 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     5701 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     5761 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     5821 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     5881 cgcgtcgact ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     5941 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc
     6001 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     6061 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     6121 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     6181 ctctagcagt acgtatagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     6241 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata
     6301 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     6361 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact
     6421 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     6481 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     6541 tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt gagtcgtatt
     6601 acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     6661 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     6721 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta
     6781 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     6841 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     6901 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     6961 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     7021 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     7081 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     7141 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     7201 acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     7261 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     7321 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     7381 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     7441 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     7501 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     7561 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     7621 cggtcgccgc atactctatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     7681 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     7741 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     7801 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     7861 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     7921 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     7981 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     8041 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     8101 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     8161 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     8221 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     8281 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     8341 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     8401 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     8461 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     8521 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     8581 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     8641 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     8701 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     8761 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     8821 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     8881 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     8941 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     9001 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     9061 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     9121 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     9181 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     9241 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     9301 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     9361 aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca
     9421 aaagctggag ctgcaagctt aa
//
