LOCUS       sequence-437864-       12227 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing human NEUROG2, ISL1 and LHX3 for 
            inducing pluripotent stem cells into cholinergic motor neurons..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12227)
  AUTHORS   Sepehrimanesh M, Ding B
  TITLE     Generation and optimization of highly pure motor neurons from human 
            induced pluripotent stem cells via lentiviral delivery of 
            transcription factors.
  JOURNAL   Am J Physiol Cell Physiol. 2020 Oct 1;319(4):C771-C780. doi: 
            10.1152/ajpcell.00279.2020. Epub 2020 Aug 12.
  PUBMED    32783653
REFERENCE   2  (bases 1 to 12227)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1152/ajpcell.00279.2020"; journalName: "Am J Physiol Cell 
            Physiol"; date: "2020-10-1- 1"; volume: "319"; issue: "4"; pages: 
            "C771-C780"
COMMENT     Sequence Label: pCSC-NGN2-IRES-ISL1-T2A-LHX3
FEATURES             Location/Qualifiers
     source          1..12227
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        385..583
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             601..781
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    828..953
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1446..1679
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1864..1908
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2160..2276
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2299..2602
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2603..2806
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2803..2827
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     complement(3888..3905)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4115..4134
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      4310..4319
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      5106..5115
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5390..5443
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      5450..5459
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    6803..7391
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6856..6876)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7274..7285)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7798..7978
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8004..8021)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8010..8234
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8280..8708
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8367..8386)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8577..8598
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8717..8737)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8722..9051
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8902..9037
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        9099..9146
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9165..9539
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9669..9790
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9706..9725)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9760..9779
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9839..9855)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9839..9855)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9852..9874)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9863..9879
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9887..9893,9894..9911,9912..9917))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9932..9953
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10070..10087)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10241..10829)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10321..10340)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11000..11791,11792..11860))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11623..11642
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11861..11965)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(12040..12059)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
      421 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      481 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
      541 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagcgcgttt tgcctgtact
      601 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
      661 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      721 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      781 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      841 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      901 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      961 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
     1021 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
     1081 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
     1141 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
     1201 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
     1261 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
     1321 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     1381 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     1441 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     1501 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     1561 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1621 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1681 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1741 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1801 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1861 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1921 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1981 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     2041 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     2101 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatcaact
     2161 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     2221 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa aattttatcg
     2281 ataagcttgg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     2341 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     2401 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     2461 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     2521 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     2581 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
     2641 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
     2701 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
     2761 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg
     2821 tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac accgactcta
     2881 gaggatccac cggtgccacc atgttcgtca aatccgagac cttggagttg aaggaggaag
     2941 aggacgtgtt agtgctgctc ggatcggcct cccccgcctt ggcggccctg accccgctgt
     3001 catccagcgc cgacgaagaa gaggaggagg agccgggcgc gtcaggcggg gcgcgtcggc
     3061 agcgcggggc tgaggccggg cagggggcgc ggggcggcgt ggctgcgggt gcggagggct
     3121 gccggcccgc acggctgctg ggtctggtac acgattgcaa acggcgccct tcccgggcgc
     3181 gggccgtctc ccgaggcgcc aagacggccg agacggtgca gcgcatcaag aagacccgta
     3241 gactgaaggc caacaaccgc gagcgaaacc gcatgcacaa cctcaacgcg gcactggacg
     3301 cgctgcgcga ggtgctcccc acgttccccg aggacgccaa gctcaccaag atcgagaccc
     3361 tgcgcttcgc ccacaactac atctgggcac tcaccgagac cctgcgcctg gcggatcact
     3421 gcgggggcgg cggcgggggc ctgccggggg cgctcttctc cgaggcagtg ttgctgagcc
     3481 cgggaggcgc cagcgccgcc ctgagcagca gcggagacag cccctcgccc gcctccacgt
     3541 ggagttgcac caacagcccc gcgccgtcct cctccgtgtc ctccaattcc acctccccct
     3601 acagctgcac tttatcgccc gccagcccgg ccgggtcaga catggactat tggcagcccc
     3661 cacctcccga caagcaccgc tatgcacctc acctccccat agccagggat tgtatctaga
     3721 tgaattccgc cccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg
     3781 tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg
     3841 gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg
     3901 aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca
     3961 aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct
     4021 ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca
     4081 cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa
     4141 ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg
     4201 cacatgcttt acatgtgttt agtcgaggtt aaaaaacgtc taggcccccc gaaccacggg
     4261 gacgtggttt tcctttgaaa aacacgataa tgctagagga tccaccggtg ccaccatggg
     4321 agacatggga gatccaccaa aaaaaaaacg tctgatttcc ctatgtgttg gttgcggcaa
     4381 tcagattcac gatcagtata ttctgagggt ttctccggat ttggaatggc atgcggcatg
     4441 tttgaaatgt gcggagtgta atcagtattt ggacgagagc tgtacatgct ttgttaggga
     4501 tgggaaaacc tactgtaaaa gagattatat caggttgtac gggatcaaat gcgccaagtg
     4561 cagcatcggc ttcagcaaga acgacttcgt gatgcgtgcc cgctccaagg tgtatcacat
     4621 cgagtgtttc cgctgtgtgg cctgcagccg ccagctcatc cctggggacg aatttgcgct
     4681 tcgggaggac ggtctcttct gccgagcaga ccacgatgtg gtggagaggg ccagtctagg
     4741 cgctggcgac ccgctcagtc ccctgcatcc agcgcggcca ctgcaaatgg cagcggagcc
     4801 catctccgcc aggcagccgg ccctgcggcc ccacgtccac aagcagccgg agaagaccac
     4861 ccgcgtgcgg actgtgctga acgagaagca gctgcacacc ttgcggacct gctacgccgc
     4921 aaacccgcgg ccagatgcgc tcatgaagga gcaactggta gagatgacgg gcctcagtcc
     4981 ccgtgtgatc cgggtctggt ttcaaaacaa gcggtgcaag gacaagaagc gaagcatcat
     5041 gatgaagcaa ctccagcagc agcagcccaa tgacaaaact aatatccagg ggatgacagg
     5101 aactcccatg gtggctgcca gtccagagag acacgacggt ggcttacagg ctaacccagt
     5161 ggaagtacaa agttaccagc caccttggaa agtactgagc gacttcgcct tgcagagtga
     5221 catagatcag cctgcttttc agcaactggt caatttttca gaaggaggac cgggctctaa
     5281 ttccactggc agtgaagtag catcaatgtc ctctcaactt ccagatacac ctaacagcat
     5341 ggtagccagt cctattgagg caaggtacaa gtccggactc agatcctacg aggggagagg
     5401 aagtcttctg acctgcggag acgtcgaaga gaatcctgga cccgctagag ccaccatgga
     5461 ggcgcgcggg gagctgggcc cggcccggga gtcggcggga ggcgacctgc tgctagcact
     5521 gctggcgcgg agggcggacc tgcgccgaga gatcccgctg tgcgctggct gtgaccagca
     5581 catcctggac cgcttcatcc tcaaggctct ggaccgccac tggcacagca agtgtctcaa
     5641 gtgcagcgac tgccacacgc cactggccga gcgctgcttc agccgagggg agagcgttta
     5701 ctgcaaggac gactttttca agcgcttcgg gaccaagtgc gccgcgtgcc agctgggcat
     5761 cccgcccacg caggtggtgc gccgcgccca ggacttcgtg taccacctgc actgctttgc
     5821 ctgcgtcgtg tgcaagcggc agctggccac gggcgacgag ttctacctca tggaggacag
     5881 ccggctcgtg tgcaaggcgg actacgaaac cgccaagcag cgagaggccg aggccacggc
     5941 caagcggccg cgcacgacca tcaccgccaa gcagctggag acgctgaaga gcgcttacaa
     6001 cacctcgccc aagccggcgc gccacgtgcg cgagcagctc tcgtccgaga cgggcctgga
     6061 catgcgcgtg gtgcaggttt ggttccagaa ccgccgggcc aaggagaaga ggctgaagaa
     6121 ggacgccggc cggcagcgct gggggcagta tttccgcaac atgaagcgct cccgcggcgg
     6181 ctccaagtcg gacaaggaca gcgttcagga ggggcaggac agcgacgctg aggtctcctt
     6241 ccccgatgag ccttccttgg cggaaatggg cccggccaat ggcctctacg ggagcttggg
     6301 ggaacccacc caggccttgg gccggccctc gggagccctg ggcaacttct ccctggagca
     6361 tggaggcctg gcaggcccag agcagtaccg agagctgcgt cccggcagcc cctacggtgt
     6421 ccccccatcc cccgccgccc cgcagagcct ccctggcccc cagcccctcc tctccagcct
     6481 ggtgtaccca gacaccagct tgggccttgt gccctcggga gcccccggcg ggcccccacc
     6541 catgagggtg ctggcaggga acggacccag ttctgaccta tccacgggga gcagcggggg
     6601 ttaccccgac ttccctgcca gccccgcctc ctggctggat gaggtagacc acgctcagtt
     6661 ctaactcgag ggatcaagct tatcgaaacc tggaaaaaca tggagcaatc acaagtagca
     6721 atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg
     6781 gttttccagt cacacctcag acaatcaacc tctggattac aaaatttgtg aaagattgac
     6841 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     6901 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     6961 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     7021 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     7081 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     7141 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct
     7201 gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     7261 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     7321 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     7381 cgcctccccg cctggaattc gagctcggta cctttaagac caatgactta caaggcagct
     7441 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     7501 cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt ccctgattgg
     7561 cagaactaca caccagggcc agggatcaga tatccactga cctttggatg gtgctacaag
     7621 ctagtaccag ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa cacccgcttg
     7681 ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtatt agagtggagg
     7741 tttgacagcc gcctagcatt tcatcacatg gcccgagagc tgcatccgga ctgtactggg
     7801 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     7861 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     7921 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagg
     7981 gccgctttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     8041 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     8101 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     8161 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     8221 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca
     8281 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     8341 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     8401 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     8461 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     8521 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     8581 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     8641 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     8701 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     8761 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     8821 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     8881 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     8941 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag
     9001 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg
     9061 ggagcttgta tatccatttt cggatctgat cagcacgtgt tgacaattaa tcatcggcat
     9121 agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatggcc aagttgacca
     9181 gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc
     9241 ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg
     9301 tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc ggacaacacc ctggcctggg
     9361 tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact
     9421 tccgggacgc ctccgggccg gccatgaccg agatcggcga gcagccgtgg gggcgggagt
     9481 tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag caggactgac
     9541 acgtgctacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     9601 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     9661 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     9721 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     9781 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt
     9841 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     9901 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     9961 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
    10021 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
    10081 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
    10141 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
    10201 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
    10261 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
    10321 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
    10381 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
    10441 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
    10501 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
    10561 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
    10621 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
    10681 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
    10741 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
    10801 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
    10861 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
    10921 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
    10981 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
    11041 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
    11101 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
    11161 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
    11221 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
    11281 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
    11341 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
    11401 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
    11461 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
    11521 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
    11581 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
    11641 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
    11701 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
    11761 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
    11821 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
    11881 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
    11941 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg
    12001 gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc
    12061 atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag
    12121 caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag
    12181 ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgc
//
