LOCUS       sequence-437869-       12209 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  a. Expresses Cre recombinase tagged with 23x repeats of MS2 binding 
            sequence under EF1A promotor and b. NLS-tdMCP_GFP reporter under UbC
            promotor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12209)
  AUTHORS   Haimovich G, Dasgupta S, Ravi AG, Gerst JE
  TITLE     Complementation of a human disease phenotype in vitro by 
            intercellular mRNA transfer
  JOURNAL   bioRxiv 2024.11.06.622258
REFERENCE   2  (bases 1 to 12209)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.11.06.622258"
COMMENT     Sequence Label: pLenti-Cre-MS2_UbC-NLS-tdMCP-GFP (Cre_tdMCP-GFP)
FEATURES             Location/Qualifiers
     source          1..12209
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     181..200
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    199..254
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     300..319
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     misc_RNA        complement(444..462)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        complement(483..501)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        complement(553..571)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        complement(592..610)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        complement(662..680)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        complement(701..719)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        complement(771..789)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        complement(810..828)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        complement(880..898)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        complement(919..937)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        complement(989..1007)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        complement(1028..1046)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        complement(1113..1131)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        complement(1152..1170)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        complement(1222..1240)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        complement(1261..1279)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        complement(1331..1349)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        complement(1401..1419)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        complement(1440..1458)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        complement(1510..1528)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        complement(1549..1567)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        complement(1619..1637)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        complement(1658..1676)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     CDS             complement(1770..2798)
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="SNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLL
                     SVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLP
                     RPSDSNAVSLVVRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFL
                     GIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVER
                     WISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQR
                     YLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLQD
                     GD"
     CDS             complement(2799..2819)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        complement(2835..4013)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2844..3782
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(2867..2887)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        4025..4423
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     CDS             4438..4458
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4462..4488
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             4462..4488
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4489..4500
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             join(5236..5238,5239..5241,5242..5955)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5236..5238,5239..5241,5242..5952)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5542..5561)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5889..5910
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5971..6559
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6024..6044)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6442..6453)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6646..6879
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6951..7072
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6988..7007)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7042..7061
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7112..7247
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7267..7286)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7268..7286)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7296..7313)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7296..7312)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7305..7327)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7454..7909
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7541..7560)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7751..7772
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7935..8039
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8040..8108,8109..8900)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8258..8277)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9071..9659
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9560..9579
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9813..9830
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9947..9968
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9983..9988,9989..10006,10007..10013)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10021..10037
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10026..10048
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10045..10061
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10045..10061
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10080..10100
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10082..10100
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        10128..10354
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             10355..10535
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10582..10707
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11200..11433
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11618..11662
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11929..12046
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 aaaaaaaatc taacctcaag tcaaggcttt tctatggaat aaggaatgga cagcaggggg
       61 ctgtttcata tactgatgac ctctttatag ccaacctttg ttcatggcag ccagcatatg
      121 ggcatatgtt gccaaactct aaaccaaata ctcattctga tgttttaaat gatttgccct
      181 cccatatgtc cttccgagtg agagacacaa aaaattccaa cacactattg caatgaaaat
      241 aaatttcctt tattagccag aagtcagatg ctcaaggggc ttcatgatgt ccccataatt
      301 tttggcagag ggaaaaagat ctcagtggta tttgtgagcc agggcattgg ccacaccagc
      361 caccaccttc tgataggcag cctgcacctg aggagtgaag gatccgatcg cgcgcagatc
      421 taatgaaccc gggaatactg cagacatggg tgatcctcat gttttctgga gtcgacctgc
      481 agacatgggt gatcctcatg ttttctaggc aattaggtac cttaggatct aatgaacccg
      541 ggaatactgc agacatgggt gatcctcatg ttttctagag tcgacctgca gacatgggtg
      601 atcctcatgt tttctaggca attaggtacc ttaggatcta atgaacccgg gaatactgca
      661 gacatgggtg atcctcatgt tttctagagt cgacctgcag acatgggtga tcctcatgtt
      721 ttctaggcaa ttaggtacct taggatctaa tgaacccggg aatactgcag acatgggtga
      781 tcctcatgtt ttctggagtc gacctgcaga catgggtgat cctcatgttt tctaggcaat
      841 taggtacctt aggatctaat gaacccggga atactgcaga catgggtgat cctcatgttt
      901 tctagagtcg acctgcagac atgggtgatc ctcatgtttt ctaggcaatt aggtacctta
      961 ggatctaatg aacccgggaa tactgcagac atgggtgatc ctcatgtttt ctggagtcga
     1021 cctgcagaca tgggtgatcc tcatgttttc taggcaayta kgtrcydbwg awcygatcgc
     1081 gcgcagatct aatgaacccg ggaatactgc agacatgggt gatcctcatg ttttctggag
     1141 tcgacctgca gacatgggtg atcctcatgt tttctaggca attaggtacc ttaggatcta
     1201 atgaacccgg gaatactgca gacatgggtg atcctcatgt tttctagagt cgacctgcag
     1261 acatgggtga tcctcatgtt ttctaggcaa ttaggtacct taggatctaa tgaacccggg
     1321 aatactgcag acatgggtga tcctcatgtt ttctaggcaa ttaggtacct taggatctaa
     1381 tgaacccggg aatactgcag acatgggtga tcctcatgtt ttctggagtc gacctgcaga
     1441 catgggtgat cctcatgttt tctaggcaat taggtacctt aggatctaat gaacccggga
     1501 atactgcaga catgggtgat cctcatgttt tctagngtcg nnctgcagac atgggtgatc
     1561 ctcatgtttt ctaggcaatt aggtacctta ggatctaatg aacccgggaa tactgcagac
     1621 atgggtgatc ctcatgtttt ctggagtcga cctgcagaca tgggtgatcc tcatgttttc
     1681 taggcaatta ggtaccttag gatcgatata tagggcccga attctgcaga tatccagcac
     1741 agtggcggcc gcggcttgat cgaaagcttc taatcgccat cttgcagcag gcgcaccatt
     1801 gcccctgttt cactatccag gttacggata tagttcatga caatatttac attggtccag
     1861 ccaccagctt gcatgatctc cggtattgaa actccagcgc gggccatatc tcgcgcggct
     1921 ccgacacggg cactgtgtcc agaccaggcc aggtatctct gaccagagtc atccttagcg
     1981 ccgtaaatca atcgatgagt tgcttcaaaa atcccttcca gggcgcgagt tgatagctgg
     2041 ctggtggcag atggcgcggc aacaccattt tttctgaccc ggcaaaacag gtagttattc
     2101 ggatcatcag ctacaccaga gacggaaatc catcgctcga ccagtttagt tacccccagg
     2161 ctaagtgcct tctctacacc tgcggtgcta accagcgttt tcgttctgcc aatatggatt
     2221 aacattctcc caccgtcagt acgtgagata tctttaaccc tgatcctggc aatttcggct
     2281 atacgtaaca gggtgttata agcaatcccc agaaatgcca gattacgtat atcctggcag
     2341 cgatcgctat tttccatgag tgaacgaacc tggtcgaaat cagtgcgttc gaacgctaga
     2401 gcctgttttg cacgttcacc ggcatcaacg ttttcttttc ggatccgccg cacaaccagt
     2461 gaaacagcat tgctgtcact tggtcgtggc agcccggacc gacgatgaag catgtttagc
     2521 tggcccaaat gttgctggat agtttttact gccagaccgc gcgcctgaag atatagaaga
     2581 taatcgcgaa catcttcagg ttctgcggga aaccatttcc ggttattcaa cttgcaccat
     2641 gccgcccacg accggcaaac ggacagaagc attttccagg tatgctcaga aaacgcctgg
     2701 cgatccctga acatgtccat caggttcttg cgaacctcat cactcgttgc atcgaccggt
     2761 aatgcaggca aattttggtg tacggtcagt aaattggaca ccttcctctt cttcttgggt
     2821 gccatgcggc cgcttcacga cacctgaaat ggaagaaaaa aactttgaac cactgtctga
     2881 ggcttgagaa tgaaccaaga tccaaactca aaaagggcaa attccaagga gaattacatc
     2941 aagtgccaag ctggcctaac ttcagtctcc acccactcag tgtggggaaa ctccatcgca
     3001 taaaacccct ccccccaacc taaagacgac gtactccaaa agctcgagaa ctaatcgagg
     3061 tgcctggacg gcgcccggta ctccgtggag tcacatgaag cgacggctga ggacggaaag
     3121 gcccttttcc tttgtgtggg tgactcaccc gcccgctctc ccgagcgccg cgtcctccat
     3181 tttgagctcc ctgcagcagg gccgggaagc ggccatcttt ccgctcacgc aactggtgcc
     3241 gaccgggcca gccttgccgc ccagggcggg gcgatacacg gcggcgcgag gccaggcacc
     3301 agagcaggcc ggccagcttg agactacccc cgtccgattc tcggtggccg cgctcgcagg
     3361 ccccgcctcg ccgaacatgt gcgctgggac gcacgggccc cgtcgccgcc cgcggcccca
     3421 aaaaccgaaa taccagtgtg cagatcttgg cccgcattta caagactatc ttgccagaaa
     3481 aaaagcgtcg cagcaggtca tcaaaaattt taaatggcta gagacttatc gaaagcagcg
     3541 agacaggcgc gaaggtgcca ccagattcgc acgcggcggc cccagcgccc aggccaggcc
     3601 tcaactcaag cacgaggcga aggggctcct taagcgcaag gcctcgaact ctcccaccca
     3661 cttccaaccc gaagctcggg atcaagaatc acgtactgca gccaggtgga agtaattcaa
     3721 ggcacgcaag ggccataacc cgtaaagagg ccaggcccgc gggaaccaca cacggcactt
     3781 acctgtgttc tggcggcaaa cccgttgcga aaaagaacgt tcacggcgac tactgcactt
     3841 atatacggtt ctcccccacc ctcgggaaaa aggcggagcc agtacacgac atcactttcc
     3901 cagtttaccc cgcgccacct tctctaggca ccggttcaat tgccgacccc tccccccaac
     3961 ttctcgggga ctgtgggcga tgtgcgctct gcccactgac gggcaccgga gcctcacgac
     4021 tagtggcctc cgcgccgggt tttggcgcct cccgcgggcg cccccctcct cacggcgagc
     4081 gctgccacgt cagacgaagg gcgcagcgag cgtcctgatc cttccgcccg gacgctcagg
     4141 acagcggccc gctgctcata agactcggcc ttagaacccc agtatcagca gaaggacatt
     4201 ttaggacggg acttgggtga ctctagggca ctggttttct ttccagagag cggaacaggc
     4261 gaggaaaagt agtcccttct cggcgattct gcggagggat ctccgtgggg cggtgaacgc
     4321 cgatgattat ataaggacgc gccgggtgtg gcacagctag ttccgtcgca gccgggattt
     4381 gggtcgcggt tcttgtttgt ggatcgctgt gatcgtcact tggcggccgc aatgggccca
     4441 aaaaagaaaa gaaaagttgg ctacccctac gacgtgcccg actacgccat cgaaggccgc
     4501 catatgctag ccgttaaaat ggcttctaac tttactcagt tcgttctcgt cgacaatggc
     4561 ggaactggcg acgtgactgt cgccccaagc aacttcgcta acgggatcgc tgaatggatc
     4621 agctctaact cgcgttcaca ggcttacaaa gtaacctgta gcgttcgtca gagctctgcg
     4681 cagaatcgca aatacaccat caaagtcgag gtgcctaaag gcgcctggcg ttcgtactta
     4741 aatatggaac taaccattcc aattttcgcc acgaattccg actgcgagct tattgttaag
     4801 gcaatgcaag gtctcctaaa agatggaaac ccgattccct cagcaatcgc agcaaactcc
     4861 ggcatctacg ccatggcttc taactttact cagttcgttc tcgtcgacaa tggcggaact
     4921 ggcgacgtga ctgtcgcccc aagcaacttc gctaacggga tcgctgaatg gatcagctct
     4981 aactcgcgtt cacaggctta caaagtaacc tgtagcgttc gtcagagctc tgcgcagaat
     5041 cgcaaataca ccatcaaagt cgaggtgcct aaaggcgcct ggcgttcgta cttaaatatg
     5101 gaactaacca ttccaatttt cgccacgaat tccgactgcg agcttattgt taaggcaatg
     5161 caaggtctcc taaaagatgg aaacccgatt ccctcagcaa tcgcagcaaa ctccggcatc
     5221 tacgcggatt ctagaatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     5281 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     5341 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     5401 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac
     5461 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     5521 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     5581 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     5641 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
     5701 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
     5761 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     5821 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag
     5881 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     5941 gagctgtaca agtaaagcgg ccgcgtcgac aatcaacctc tggattacaa aatttgtgaa
     6001 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     6061 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     6121 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     6181 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     6241 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     6301 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     6361 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg
     6421 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     6481 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     6541 ctttgggccg cctccccgcc tggaattcga gctcggtacc tttaagacca atgacttaca
     6601 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     6661 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     6721 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     6781 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     6841 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta
     6901 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
     6961 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     7021 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     7081 ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     7141 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     7201 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc
     7261 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt
     7321 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     7381 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     7441 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     7501 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     7561 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     7621 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     7681 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     7741 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     7801 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     7861 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt
     7921 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     7981 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     8041 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     8101 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     8161 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     8221 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     8281 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     8341 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     8401 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     8461 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     8521 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     8581 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     8641 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     8701 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     8761 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     8821 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     8881 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     8941 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     9001 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     9061 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     9121 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     9181 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     9241 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     9301 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     9361 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     9421 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     9481 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     9541 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     9601 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     9661 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     9721 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     9781 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     9841 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     9901 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     9961 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
    10021 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
    10081 caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc
    10141 aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga
    10201 gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg
    10261 aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga
    10321 tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg
    10381 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
    10441 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
    10501 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
    10561 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
    10621 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
    10681 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
    10741 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
    10801 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
    10861 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
    10921 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
    10981 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
    11041 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
    11101 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
    11161 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
    11221 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
    11281 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
    11341 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
    11401 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
    11461 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
    11521 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
    11581 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
    11641 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
    11701 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
    11761 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
    11821 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
    11881 agacagatcc attcgattag tgaacggatc tcgacggtat cggttaactt ttaaaagaaa
    11941 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
    12001 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg atcacgagac
    12061 tagcctcgag aagcttgata tcatctccat aagagaagag ggacagctat gactgggagt
    12121 agtcaggaga ggaggaaaaa tctggctagt aaaacatgta aggaaaattt tagggatgtt
    12181 aaagaaaaaa ataacacaaa acaaaatat
//
