LOCUS       sequence-437873-       14519 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transcriptional reporter for ARF7 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14519)
  TITLE     Bishopp Lab ARF7 Reporter Plasmids 2024
REFERENCE   2  (bases 1 to 14519)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pro::EX1:GFP
FEATURES             Location/Qualifiers
     source          1..14519
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    46..68
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     rep_origin      195..630
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     CDS             921..1709
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1880..2468
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2369..2388
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    2559..2583
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      complement(2679..3386)
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     protein_bind    3405..3429
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5232..5287
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     protein_bind    complement(5309..5333)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(10372..10374,10375..10377,10378..11088)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(10417..10438)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(10678..10697)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     11025..11046
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      11397..12038
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     CDS             12721..13749
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     terminator      13757..14464
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     promoter        complement(14497..14515)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(14498..14515)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 tctagaccaa gcatgcacag ctggcgttct cagccagatc caacgttaca ccacaatata
       61 tcctgccaag atctaattcc ggggatcgga aatccagaag cccgagaggt tgccgccttt
      121 cgggcttttt ctttttcaaa aaaaaaaatt tataaaacga tctgttgcgg ccggccgccg
      181 ggttgtgggc aaaggcgctc gacggtgggc aaccgcttgc ggttgtccac gggcggagcc
      241 ggtgcgcgta gcgcattgtc cacaagccaa gggcgaccaa taattgatat atatattcat
      301 aattgaaaag ctaattgaac atactacttg ctgtaactac ttgccggagc gaggggtgtt
      361 tgcaagctgt tgatctgaaa gggctattag cgttctcacg tgcctttttg attagcgatt
      421 tcacgtgacc ttattagcga tttcacgtac tccgattagc gatttcacgt accctgatta
      481 gcgatttcac gtggatagtt tttggagcgg gccggaaagc cccgtgaatc aaggctttgc
      541 ggggcattag cggtttcacg tggataacta ccctctatcc acaggcttcc ggggataaaa
      601 aagcccgctc gacggcgggc tgttggatgg ggatcgcctg aatcgcccca tcatccagcc
      661 agaaagtgag ggagccacgg ttgatgagag ctttgttgta ggtggaccag ttggtgattt
      721 tgaacttttg ctttgccacg gaacggtctg cgttgtcggg aagatgcgtg atctgatcct
      781 tcaactcagc aaaagttcga tttattcaac aaagccacgt tgtgtctcaa aatctctgat
      841 gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa
      901 acagtaatac aaggggtgtt atgagggaag cggtgatcgc cgaagtatcg actcaactat
      961 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt
     1021 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg
     1081 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt
     1141 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg
     1201 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc
     1261 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct
     1321 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct
     1381 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga
     1441 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt
     1501 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg
     1561 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc taggcaggct tatcttggac
     1621 aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgttcac tacgtgaaag
     1681 gcgagatcac caaggtagtc ggcaaataat cactagacca atgttacaca tatatacttt
     1741 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1801 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1861 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1921 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttc
     1981 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     2041 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     2101 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     2161 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     2221 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     2281 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     2341 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     2401 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     2461 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     2521 ctcacatgag atctcaaaca aacacataca gcgacttagt ttacccgcca atatatcctg
     2581 tcaaggctcg aactaggctc ggacgaagta agcggccgca accttaatta aacacggcgc
     2641 gccaacagcg atcgcaccag gtaccttgca tgccggtcct gctgagcctc gacatgttgt
     2701 cgcaaaattc gccctggacc cgcccaacga tttgtcgtca ctgtcaaggt ttgacctgca
     2761 cttcatttgg ggcccacata caccaaaaaa atgctgcata attctcgggg cagcaagtcg
     2821 gttacccggc cgccgtgctg gaccgggttg aatggtgccc gtaactttcg gtagagcgga
     2881 cggccaatac tcaacttcaa ggaatctcac ccatgcgcgc cggcggggaa ccggagttcc
     2941 cttcagtgag cgttattagt tcgccgctcg gtgtgtcgta gatactagcc cctggggcac
     3001 ttttgaaatt tgaataagat ttatgtaatc agtcttttag gtttgaccgg ttctgccgct
     3061 ttttttaaaa ttggatttgt aataataaaa cgcaattgtt tgttattgtg gcgctctatc
     3121 atagatgtcg ctataaacct attcagcaca atatattgtt ttcattttaa tattgtacat
     3181 ataagtagta gggtacaatc agtaaattga acggagaata ttattcataa aaatacgata
     3241 gtaacgggtg atatattcat tagaatgaac cgaaaccggc ggtaaggatc tgagctacac
     3301 atgctcaggt tttttacaac gtgcacaaca gaattgaaag caaatatcat gcgatcatag
     3361 gcgtctcgca tatctcatta aagcaggact ctagaggatc atcaaccact ttgtacaaga
     3421 aagctgggtc ggcgcgccca cccttttact tgtacaactc gtccataccg tagaggaaga
     3481 ggtggtggcg gccctcggaa cgctcgtact gttcgacaat ggtgtaatcc tcgttgtggg
     3541 aggtaatgtc caacttggta tcaacgtagt aataaccagg caattgaacg ggtttcttgg
     3601 ccatgtagat ggtcttaaac tccacgaggt aatgtccacc gtccttcaac ttcaatgcct
     3661 ggtgaatctc gcccttcaac actccatcgc ggggatacaa gcgctctgtt gaagcctccc
     3721 agcccatagt cttcttctgc ataactgggc cgtctggtgg gaaattggtt ccacgcattt
     3781 tgaccttgta gataagcgta ccatcttgaa gggaggagtc ttgggtgact gtaaccaaac
     3841 caccgtcctc gaagttcata acgcgctccc acttaaatcc ttctgggaaa gacaacttct
     3901 tataatctgg aatatcggct gggtgtttca cgtaagcctt ggaaccgtac ataaactgtg
     3961 gtgagagaat atcccaagcg aaaggaagtg gtccaccctt ggtgactttc aacttagctg
     4021 tctgagtgcc ctcatatggg cggccctcac cctctccttc gatctcgaac tcgtggccat
     4081 tcattgagcc ctccatacgg actttgaagc acataaactc cttgatcaca gccatgttat
     4141 tatcctcgga tgaagcggtg ccagagctgc cactaccggt gcttccagta ccatgaccca
     4201 agaaaaggtg atggcgaccc tcagacctct catattgttc aacgatggtg tagtcctcgt
     4261 tgtgggaggt gatatcgagt ttagtgtcca cgtagtaata gccggggagt tgcacgggct
     4321 tcttagccat gtagatggtc ttgaactcca ccaagtagtg gcctccatcc ttaagtttga
     4381 gagcttggtg gatttcaccc ttaagaacgc cgtctcttgg gtaaagcctc tcagtggagg
     4441 cttcccaacc catagttttc ttttgcatga ctggaccatc gggaggaaag tttgtgcctc
     4501 tcatcttcac cttgtagatc aaagtgccgt cctgcaaact agaatcttgg gtcacagtca
     4561 caagacctcc atcctcgaag ttcatgaccc tttcccactt gaaaccctct gggaaggaca
     4621 actttttgta atcggggatg tcagcaggat gcttaacgta tgccttagag ccatacatga
     4681 attgggggga caagatgtcc caagcgaagg gcaaaggacc acccttagtc actttgagct
     4741 ttgcggtttg ggtgccctcg tagggtctac cttcaccctc gccctcgatc tcgaactcgt
     4801 gaccgttcat ggaaccttcc atcctaacct tgaatctcat gaactctttg ataacctcct
     4861 cacccttgga aaccatggat cgggctgccg cagcggcagc agccgcagca ggtttggtga
     4921 ggacataaat ggcgtatatg atcccaggaa tatacccaag tagcgtcaaa accaaacata
     4981 tccaaaactc aacctgcaac aaaattcata atttcaactt ttaatttcta gtgtggataa
     5041 atgtaaaaaa agaaccatat ttgactaaag tataagatta attaaccaaa ataaaactca
     5101 gtctaaatat gagaggaaag ataaataccc cgcaaccaaa tctgagaaag acaccgagtg
     5161 gaggcaagag gatggcgata ataatatcaa cgaaagtagc tgtactcatt ttggtgaagg
     5221 gggcggccgc tgtaattgta aatagtaatt gtaatgttgt ttgttgtttg ttgttgttgg
     5281 taattgttgt aaaaatacgc ggccgcggag cctgcttttt tgtacaaact tgttgatgat
     5341 ccctgttaat cagaaaaact cagattaatc gacaaattcg atcgcacaaa ctagaaacta
     5401 acaccagatc tagatagaaa tcacaaatcg aagagtaatt attcgacaaa actcaaatta
     5461 tttgaacaaa tcggatgata tctatgaaac cctaatcgag aattaagatg atatctaacg
     5521 atcaaaccca gaaaatcgtc ttcgatctaa gattaacaga atctaaacca aagaacatat
     5581 acgaaattgg gatcgaacga aaacaaaatc gaagattttg agagaataag gaacacagaa
     5641 atttaccttg atcacggtag agagaattga gagaaagttt ttaagatttt gagaaattga
     5701 aatctgaatt gtgaagaaga agagctcttt gggtattgtt ttatagaaga agaagaagaa
     5761 aagacgagga cgactaggtc acgagaaagc taaggcggtg aagcaatagc taataataaa
     5821 atgacacgtg tattgagcgt tgtttacacg caaagttgtt tttggctaat tgccttattt
     5881 ttaggttgag gaaaagtatt tgtgctttga gttgataaac acgactcgtg tgtgccggct
     5941 gcaaccactt tgacgccgtt tattactgac tcgtcgacaa ccacaatttc taacggtcgt
     6001 cataagatcc agccgttgag atttaacgat cgttacgatt tatatttttt tagcattatc
     6061 gttttatttt ttaaatatac ggtggagctg aaaattggca ataattgaac cgtgggtccc
     6121 actgcattga agcgtatttc gtattttcta gaattcttcg tgctttattt cttttccttt
     6181 ttgttttttt ttgccattta tctaatgcaa gtgggcttat aaaatcagtg aatttcttgg
     6241 aaaagtaact tctttatcgt ataacatatt gtgaaattat ccatttcttt taatttttta
     6301 gtgttattgg atatttttgt atgattattg atttgcatag gataatgact tttgtatcaa
     6361 gttggtgaac aagtctcgtt aaaaaaggca agtggtttgg tgactcgatt tattcttgtt
     6421 atttaattca tatatcaatg gatcttattt ggggcctggt ccatatttaa cactcgtgtt
     6481 cagtccaatg accaataata ttttttcatt aataacaatg taacaagaat gatacacaaa
     6541 acattctttg aataagttcg ctatgaagaa gggaacttat ccggtcctag atcatcagtt
     6601 catacaaacc tccatagagt tcaacatctt aaacaagaat atcctgatct gaagaatgtg
     6661 gaggctttag tcccttggat acttgggagg ctgtggaaga acagaaacga gctggtgctt
     6721 aaagggaggg aatttggaac caatgaggta ttagtaagga cacaagaaga tgcagatgag
     6781 tggattagaa ggaaagaggc tcagaatgta aggaaagcac caaccacaac accgtcggga
     6841 cagacgagaa acatgggaag ctccaccaca atcttgggtt aagtgcaact ttgatggggc
     6901 atggccaaca gaaggattaa aatgtggctt agggtgggtg cttcgcgatc atacagggaa
     6961 ggtgttatgg ttaggtgcaa gagctgtggt aaaagtaaga agcgtgctgg aagtagaagt
     7021 ggaggctctt agatgggctg tgctgtcatt atcccgattc aattatagga agatcatttt
     7081 tgaggtggat tctcagcaac ttgtgtcttt ggttacatga aagttatgct tgtcaagtct
     7141 caatccaatt atccaagaca taaagtatct acttagcaag tttgaggatt ttatgcttgt
     7201 gcatacaagc cgagaaggaa atggagtggc agatagaata gctaaggaat ctctttcttt
     7261 tgagaattat gatctaaagt tgtattctat tgtaccaatt tgggttaaaa gctctgttga
     7321 gctagactca tatgcatacc taagagatgt cgcaaaccag ctcatgtcac caaacaacaa
     7381 ttagagccaa tgtgaaatgg ggtgggagtg tgaccacgtc aggtgggatt tgccgtgaga
     7441 ggcccttctt tattgtttgt tcctttttct tcaattatta ttttcaataa taatgtaatt
     7501 aaacttatca tcaaatacaa atattcgata aattaaagga tcctctacaa gaaatatgaa
     7561 tattgataac acataagaat agcaaaattt ttgtaactat tattaaaaat aatattcgag
     7621 atttgttaac catagtataa catttgtaaa ccactaaaca taaaagtaac gagaaaatct
     7681 cctaaagcaa agtaacgttt ctctatattt agcgttttac aaaatttatg ttaaaaaatt
     7741 atgtttagcg ttttacaaaa attatgctag aaaagtttat gtttagcgga aatctaaaat
     7801 cacttaaaac gcaaaaaatt aatcagtacg tagattattt tccacaactc tctcttacat
     7861 acaaattaac aaataatgca tatcagaatg acatagtaaa gtcgagaaaa tcatggaatc
     7921 gtaacgaaac ttcctcgtct ctctctgata attcatccaa ctcaagtggt gtattttgtg
     7981 gttacattta catatattgt caacaccaac tgtcgaattc attaaatcgg ctaaaatacg
     8041 ttgatgtgat tctggaaata tctttaggac agtatatgag ttattcatct tgttcatttt
     8101 ccactttcca ctgcggacac catacggaca cacaaccggt tctgaaatgc gtcacgctct
     8161 tcttcctttc attccaactt taatacatgc atgaagtcgt agttcggagt tcatactaaa
     8221 tttcggtcac tttgtaataa gaaaactatt aacatttttc tttttctttt tttaaacgtt
     8281 agctaggcct caaagtttca caaaaaaaaa agaaaaaaaa aagctaggcg tcgaaatcca
     8341 taacttcaaa aggccttctt acattcatcc aatctccaaa cttctgtctt cacgcattta
     8401 ttgctcaggg acatcatgtc gctactcact gccacttatt tgaattgaca tccattcatt
     8461 ccatacatat atattacctt cacttaaagt tgtaagtata tagaatttta agcattcctg
     8521 ccaaataagt tttggatcga acctaaacta acaaagaggt tactgttttg taactttctt
     8581 ttttgatgtg gggtcattca aagggtttac atgcatttta caatggtctg tgatgaatat
     8641 ggaggttcag tctttggtag ataaaacttg ttatgtggcc ttaaaccaaa tagattgtag
     8701 tttgtacaat gacatattta tggttatatt cggttttcca atatgtttag gaatatatat
     8761 caccgttttt ttttgggtag acattttaac gattatagag gaaaattttg aatgatggct
     8821 gtactgggaa aagagtgtaa gttgtgatca tcatatgtaa ttttattttg tgtgacatta
     8881 taacacttct tccaaaagac aaaagtttag gttaattaat ataagaaacc aataaagaat
     8941 catgaacaaa gagagaactt ctatcatttt tgatatgaaa tcgtccgtta aaatattgtt
     9001 ctttgtgatc gcatatgctg tatgtactta gatttgatca attaatcaac atttacaaca
     9061 aaataagatc aattgtttat aatgatgatc taagcgaatt tagatttgtt tgttattata
     9121 gaactcatta ctaaacattt aagttccatt ctcgtttaaa acatgaacgc tagtttatga
     9181 aactcagaac caaccatgta aaactccaca aaatttctag taaaatgctt ttgttcaaaa
     9241 taatgaaaat cataaaattt tttgatgaat tatgaatcct aaaatctatc tgaattaaat
     9301 atagttaact acaaaagaaa ttgccaaaca tatatgtacg cactttgatc aaataactag
     9361 ctagtcagct tagtttgttt ttttcaatac atcccctata aatctatgtt atccctaatc
     9421 aagtattaca ataaatgata cagtaataat aaccttaatg aaaaaaactt atcaaattat
     9481 cagagataag gaaaaagaaa acactaataa agttgttttt ttgtgtgtca aagttaaaat
     9541 tttagaggac aataataaca ttaagaaatt aaatcgtggc cagtttgcta gtgaatatgt
     9601 cggcttcatg agcataacta aataggtata aaaaaataaa aatatcggaa aaagaaaaga
     9661 gaaggaaaaa aaaaggagag gaagaagaaa aagctgttcc tgtgctttag cagacctttt
     9721 ggtagttgtc ctggtcgctg atcatccctt tccgacaact ctgttgctct ttctctcacg
     9781 tttgagtctt aatcttcgat tgtccttggt catccttaag ttttgttttt ctctttcgat
     9841 tcattttcca gatttctttt tatagaaacc cgtctctttg atttgattcc ccccaagaaa
     9901 ggaaagcaaa acaaagaaga gcagctgaat caagtaactt tgtgggtgtt tttgaattat
     9961 ctgactcgag ataaagagct tcgttaaaaa cggaatcttt tttaaggtcg tatgctttgt
    10021 ttgtctctcc ggagattccg cgtgtgaaaa gtcgtggttt ttgatctctt tgagatactt
    10081 aaaatttccg agaaattggc ggctctgtcg gagataaaaa gctgagatct tggagttccc
    10141 catttctgat taacgattct ctctgctgtt acttgagcta ggaagctatt aacaacttga
    10201 gtgaaagttt agggcttttg atgaagctct cacgcaaaga gcttagcttt gtataagctc
    10261 agattcagat tatttattgg gtttattctt cagagaaagt aaagttgagt gatcaacacc
    10321 atgaaagctc cttcatcaaa tggagtttct cctaatcctg ttgaaggctc catggtgagc
    10381 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
    10441 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
    10501 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
    10561 accttcacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac
    10621 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
    10681 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
    10741 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
    10801 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag
    10861 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
    10921 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
    10981 acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
    11041 ttcgtgaccg ccgccgggat cactcacggc atggacgagc tgtacaagat tgctgcagcg
    11101 gccgaattca agcgtgaaga gcaagcaagg aaagctaagg tgaacaatga gaaaaagacg
    11161 gaaatagtga aaccagagag ttgtagcaat gaaggagatg tcaaggatct gaaaagaaag
    11221 gactctgagg atggaaacga gggtgaggaa gaagaagctt cttcgaaacc gaaaaagcca
    11281 aaagttgctc tttctcatct tcaggacatt gacgacacag aagctgatca agaagaagag
    11341 tcaggtggat cctagataac ctttaccttc attccctgta aattggtacc ctgcaaagct
    11401 ttcgttcgta tcatcggttt cgacaacgtt cgtcaagttc aatgcatcag tttcattgcg
    11461 cacacaccag aatcctactg agtttgagta ttatggcatt gggaaaactg tttttcttgt
    11521 accatttgtt gtgcttgtaa tttactgtgt tttttattcg gttttcgcta tcgaactgtg
    11581 aaatggaaat ggatggagaa gagttaatga atgatatggt ccttttgttc attctcaaat
    11641 taatattatt tgttttttct cttatttgtt gtgtgttgaa tttgaaatta taagagatat
    11701 gcaaacattt tgttttgagt aaaaatgtgt caaatcgtgg cctctaatga ccgaagttaa
    11761 tatgaggagt aaaacacttg tagttgtacc attatgctta ttcactaggc aacaaatata
    11821 ttttcagacc tagaaaagct gcaaatgtta ctgaatacaa gtatgtcctc ttgtgtttta
    11881 gacatttatg aactttcctt tatgtaattt tccagaatcc ttgtcagatt ctaatcattg
    11941 ctttataatt atagttatac tcatggattt gtagttgagt atgaaaatat tttttaatgc
    12001 attttatgac ttgccaattg attgacaaca tgcatcaatc gactagtcga cgagtcagta
    12061 ataaacggcg tcaaagtggt tgcagccggc acacacgagt cgtgtttatc aactcaaagc
    12121 acaaatactt ttcctcaacc taaaaataag gcaattagcc aaaaacaact ttgcgtgtaa
    12181 acaacgctca atacacgtgt cattttatta ttagctattg cttcaccgcc ttagctttct
    12241 cgtgacctag tcgtcctcgt cttttcttct tcttcttcta taaaacaata cccaaagagc
    12301 tcttcttctt cacaattcag atttcaattt ctcaaaatct taaaaacttt ctctcaattc
    12361 tctctaccgt gatcaaggta aatttctgtg ttccttattc tctcaaaatc ttcgattttg
    12421 ttttcgttcg atcccaattt cgtatatgtt ctttggttta gattctgtta atcttagatc
    12481 gaagacgatt ttctgggttt gatcgttaga tatcatctta attctcgatt agggtttcat
    12541 agatatcatc cgatttgttc aaataatttg agttttgtcg aataattact cttcgatttg
    12601 tgatttctat ctagatctgg tgttagtttc tagtttgtgc gatcgaattt gtagattaat
    12661 ctgagttttt ctgattaaca ccacaccaat ctggtaccag aaaaggggag aagcaaaacc
    12721 atgggtaaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc
    12781 gacagcgtct ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc
    12841 gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa
    12901 gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac
    12961 attggggcat tcagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg
    13021 ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc aaccggtcgc ggaggcgatg
    13081 gatgccatcg ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa
    13141 ggaatcggtc aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg
    13201 tatcactggc aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat
    13261 gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc
    13321 ggctccaaca atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag
    13381 gcgatgttcg gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg
    13441 gcttgtatgg agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg
    13501 ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt
    13561 gacggcaatt tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc
    13621 ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat
    13681 ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca
    13741 aaggaataga tgcatcctgc tttaatgaga tatgcgagac gcctatgatc gcatgatatt
    13801 tgctttcaat tctgttgtgc acgttgtaaa aaacctgagc atgtgtagct cagatcctta
    13861 ccgccggttt cggttcattc taatgaatat atcacccgtt actatcgtat ttttatgaat
    13921 aatattctcc gttcaattta ctgattgtac cctactactt atatgtacaa tattaaaatg
    13981 aaaacaatat attgtgctga ataggtttat agcgacatct atgatagagc gccacaataa
    14041 caaacaattg cgttttatta ttacaaatcc aattttaaaa aaagcggcag aaccggtcaa
    14101 acctaaaaga ctgattacat aaatcttatt caaatttcaa aagtgcccca ggggctagta
    14161 tctacgacac accgagcggc gaactaataa cgctcactga agggaactcc ggttccccgc
    14221 cggcgcgcat gggtgagatt ccttgaagtt gagtattggc cgtccgctct accgaaagtt
    14281 acgggcacca ttcaacccgg tccagcacgg cggccgggta accgacttgc tgccccgaga
    14341 attatgcagc atttttttgg tgtatgtggg ccccaaatga agtgcaggtc aaaccttgac
    14401 agtgacgaca aatcgttggg cgggtccagg gcgaattttg cgacaacatg tcgaggctca
    14461 gcaggaccgg catgcaagct agcttactag tgatattcta tagtgtcacc taaatgtat
//
