LOCUS       sequence-437874-       11119 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac transposon plasmid for doxycycline inducible expression of 
            KDM5B.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11119)
  AUTHORS   Pierson Smela M, Adams J, Ma C, Breimann L, Widocki U, Shioda T, 
            Church GM
  TITLE     Induction of Meiosis from Human Pluripotent Stem Cells
  JOURNAL   bioRxiv 2024.05.31.596483
REFERENCE   2  (bases 1 to 11119)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.31.596483"
COMMENT     Sequence Label: pPB-cT3G-cERP2-KDM5B
FEATURES             Location/Qualifiers
     source          1..11119
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    17..35
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    53..71
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    89..107
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    125..143
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    161..179
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    197..215
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    233..251
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     278..302
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    368..392
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      393..402
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             729..740
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(5034..5058)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5060..5086
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    5253..5387
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   5659..5693
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(5704..5721)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5704..5720)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5713..5735)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5929..5948)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6050..6072
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6111..6129)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6197..6301
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6302..6370,6371..7162)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6520..6539)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7333..7921
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7822..7841
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8075..8092
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8298..8319
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8334..8339,8340..8357,8358..8364)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8372..8388
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8377..8399
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8396..8412
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8396..8412
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   8438..8500
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    8676..8757
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(9474..9530)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(9540..10283)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(10284..10307)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      10307..10316
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(10336..10547)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     enhancer        10548..10927
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atcacctcga gtttactccc tatcagtgat agagaacgta tgaagagttt actccctatc
       61 agtgatagag aacgtatgca gactttactc cctatcagtg atagagaacg tataaggagt
      121 ttactcccta tcagtgatag agaacgtatg accagtttac tccctatcag tgatagagaa
      181 cgtatctaca gtttactccc tatcagtgat agagaacgta tatccagttt actccctatc
      241 agtgatagag aacgtataag cttgcctata taagcagagc tcgtttagtg aaccgtcaga
      301 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca
      361 gcctaccaca agtttgtaca aaaaagcagg ctgccaccat ggaagctgca actacgttac
      421 atcctggacc cagacccgct cttccgctgg gtggacccgg acctctcgga gaatttcttc
      481 ccccgccaga atgtcctgtg tttgagccaa gttgggaaga atttgccgat ccttttgcct
      541 ttattcataa aattagacca atcgctgaac aaacaggtat ttgcaaagtc cgcccacccc
      601 ctgactggca accgcccttc gcttgcgacg tggacaagct ccacttcacc ccgaggattc
      661 aaaggctcaa cgagctggaa gcgcagacca gagtgaagct caactttctg gatcaaatag
      721 cgaaatattg ggaattgcaa ggttccacct taaagatccc tcacgttgaa cgcaaaattc
      781 tcgatctgtt ccaattaaac aaactggtgg ctgaggaggg agggttcgcc gtcgtatgta
      841 aagacaggaa gtggacaaag atcgcaacca aaatgggatt cgccccagga aaggccgtcg
      901 gttcccacat acgtggccac tacgagcgga tattgaatcc ttataatctc tttctcagcg
      961 gggattcact gcggtgcctt caaaagccca atctcacgac cgataccaaa gataaagaat
     1021 ataagcctca cgacatccca caacgacaat cagtccaacc gtctgagaca tgtccgcccg
     1081 ctcggcgggc gaagcggatg cgtgctgaag ctatgaacat aaagatcgag ccagaagaaa
     1141 ctactgaggc tcggacccac aaccttcgcc gaaggatggg gtgccctacc cctaagtgcg
     1201 agaacgaaaa ggagatgaaa tccagcatta aacaggagcc catcgaacgg aaggactaca
     1261 tcgtcgagaa cgagaaagag aaacctaaat caaggtcaaa gaaggcaact aacgcggtcg
     1321 atttatacgt gtgcttgctg tgcgggtctg ggaacgacga ggatcgcctg ttactgtgcg
     1381 acggatgcga cgattcctat cacactttct gtcttattcc gccacttcac gacgtaccta
     1441 aaggtgattg gagatgcccc aaatgcctcg cacaagagtg ctccaaaccc caggaagcgt
     1501 tcggtttcga gcaggccgca agagattaca ctttgcgcac cttcggagag atggccgacg
     1561 catttaagtc cgactatttt aatatgcctg ttcacatggt accgaccgaa ctggtggaaa
     1621 aggagttctg gcgtctggtc tctaccatcg aagaagacgt taccgtcgag tacggggcag
     1681 atatcgcttc caaagagttc ggtagcggtt tcccagtgag agacggaaag attaagctga
     1741 gtccggaaga ggaagaatac ctggacagcg gatggaacct gaataatatg cctgttatgg
     1801 aacaaagcgt actcgcccac atcacagccg acatctgcgg aatgaagctg ccctggctgt
     1861 acgtagggat gtgtttcagt agcttttgct ggcatatcga ggatcattgg tcttacagca
     1921 tcaattatct gcattggggc gaaccgaaga cttggtacgg ggttcctgga tacgccgccg
     1981 aacaactgga gaacgtcatg aagaagctcg cacctgagct gttcgtctca caacccgact
     2041 tgctgcacca actggtcaca attatgaatc caaacacatt gatgacgcac gaggttccag
     2101 tctatcgtac gaaccaatgc gccggagaat tcgtcatcac tttcccccgg gcgtatcatt
     2161 ctggcttcaa tcaagggttc aacttcgccg aagccgtgaa cttttgtacg gtagactggc
     2221 tccctctggg tagacaatgc gtcgaacact acagacttct gcacagatac tgcgtattca
     2281 gtcatgacga aatgatatgt aaaatggcct caaaagccga cgtgttggac gtcgttgtcg
     2341 ccagtaccgt gcaaaaggat atggctatca tgatcgaaga cgaaaaggcc ctgcgggaga
     2401 ccgtacggaa gctcggggtc atagacagcg agaggatgga cttcgaactt ctccccgacg
     2461 acgagcgaca atgcgtcaag tgtaagacga cttgttttat gtcagcaatt agctgctcct
     2521 gcaagccggg gttgttagta tgtcttcacc acgtgaagga gttatgctct tgcccaccat
     2581 ataagtacaa gctgcgatac cgatatactc ttgacgacct gtatccaatg atgaacgccc
     2641 tcaaactcag agcggagtcc tataatgagt gggctctgaa cgtaaacgag gcactggaag
     2701 ccaaaattaa taagaagaag tccctggtgt cttttaaagc ccttatcgag gagagtgaga
     2761 tgaagaagtt tcctgataac gacctgttac ggcatctgag gctggttacc caagacgctg
     2821 aaaagtgcgc tagcgtggcc caacaattac tgaacggaaa gcgccagaca cgatacagaa
     2881 gtgggggggg taagagccag aaccaactga ccgttaacga acttcgtcaa ttcgtcactc
     2941 aattgtacgc cttgccttgc gtgctgagcc aaactcccct tcttaaagac ctgctgaacc
     3001 gcgtggaaga cttccagcaa cactcacaaa agctcttatc agaagagacc cccagcgccg
     3061 cagaacttca agatctgctg gacgtatcat tcgagttcga cgtggagctg cctcaactgg
     3121 ccgaaatgag gattcgcctg gagcaggcgc ggtggcttga ggaagttcaa caggcctgtt
     3181 tagatcctag tagcctgacg ctggacgaca tgcgccgcct tattgatctt ggcgtgggtc
     3241 tcgcacctta ctccgctgta gaaaaggcca tggcaaggct tcaagagttg ttaaccgtta
     3301 gcgaacattg ggatgataag gctaaaagcc tgttaaaagc gcggcctcgc cactccctta
     3361 acagtctggc cacagctgtg aaagagattg aggaaattcc agcctatctc cctaacggcg
     3421 ctgcgttgaa ggatagtgtc caacgggcgc gcgattggct gcaagacgtg gaagggttgc
     3481 aagccggcgg cagggttcct gttcttgata ctcttatcga gctcgtcacc cgcgggcgta
     3541 gcataccggt gcaccttaac tcacttcctc ggttggagac tctggtggcc gaagtgcaag
     3601 cctggaagga gtgcgccgta aacacgtttc tcacagaaaa cagtccttac tccctgcttg
     3661 aagtcctctg cccaaggtgc gacatcggtc tgctgggctt aaagagaaaa caacgaaaac
     3721 tcaaagaacc actgcccaac gggaagaaga agtctactaa gctggaaagc ttaagcgatt
     3781 tggaaagggc gctgacagag tctaaagaaa ccgccagcgc catggctaca ctgggagagg
     3841 cccggctccg agagatggaa gctcttcaat ccctgcgtct tgcaaacgag ggcaagctgc
     3901 tctcaccgct gcaggacgtt gacattaaga tatgtctgtg ccagaaagca cctgccgcgc
     3961 ccatgatcca gtgcgagctg tgtagagacg cgtttcatac atcatgcgtt gctgtgccat
     4021 caatcagtca aggacttcgc atttggttgt gcccgcactg ccgcagaagt gaaaagccgc
     4081 ccctggaaaa gatcttgcct ttgcttgctt ctttgcaaag aatacgcgtc cggctcccag
     4141 aaggggacgc cttgaggtac atgatagaga ggacagtaaa ttggcaacat agggcacaac
     4201 agttactgtc atccggaaac ctgaagttcg ttcaggacag agttgggtct ggcctcttat
     4261 actcacgatg gcaggcaagc gccgggcaag tctctgatac caataaagtg agtcagccac
     4321 caggaactac gtccttctcc cttcccgacg attgggataa tcggacgagt tacctgcata
     4381 gcccatttag cacaggtagg tcctgcattc ctctgcacgg ggtgagccct gaggtaaacg
     4441 agcttttaat ggaagcgcaa ttacttcaag tgtcactccc agagatccaa gagttgtatc
     4501 aaacactgtt agccaaaccc tcaccagccc aacaaacaga tcgctcaagc cccgtacgcc
     4561 caagtagcga gaagaacgat tgctgtagag gtaaacgcga cggtatcaat agcctggaaa
     4621 ggaagcttaa acggagattg gagcgagaag ggctgtcttc agaacgctgg gagcgcgtga
     4681 agaagatgag gacacctaag aagaagaaga ttaagctttc tcatcctaaa gatatgaata
     4741 actttaaact tgaacgcgaa cgaagttacg agttggtacg aagcgccgag acacacagtc
     4801 tccctagcga tacctcttac agtgagcaag aggatagcga agacgaggac gctatatgtc
     4861 cggccgtctc ctgtcttcaa cctgagggag acgaagttga ttgggttcaa tgcgacggta
     4921 gttgtaacca atggttccac caagtttgcg taggggtatc tcctgaaatg gccgagaaag
     4981 aggattatat atgcgttcgt tgcaccgtca aagatgcccc ctctcgcaaa tagacccagc
     5041 tttcttgtac aaagtggtat acccatacga tgttccagat tacgcttagt aatgagaaat
     5101 actcagaaga tgtcactcac catagtccgc agtgaaagat aaatgatcac tgcgctgtct
     5161 ttttcaaagt tttagagcta gaaatagcaa gtaggccact gagcaagaac tatgaatgag
     5221 atctttagac atattgcggc cgctttctcc gcgatccaga catgataaga tacattgatg
     5281 agtttggaca aaccaaaact agaatgcagt gaaaaaaatg ccttatttgt gaaatttgtg
     5341 atgctattgc cttatttgta accattataa gctgcaataa acaagttatc tataacaaga
     5401 aaatatatat ataataagtt atcacgtaag tagaacatga aataacaata taattatcgt
     5461 atgagttaaa tcttaaaagt cacgtaaaag ataatcatgc gtcattttga ctcacgcggt
     5521 cgttatagtt caaaatcagt gacacttacc gcattgacaa gcacgcctca cgggagctcc
     5581 aagcggcgac tgagatgtcc taaatgcaca gcgacggatt cgcgctattt agaaagagag
     5641 agcaatattt caagaatgca tgcgtcaatt ttacgcagac tatctttcta gggttaagaa
     5701 ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     5761 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     5821 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct
     5881 ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc
     5941 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
     6001 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accatgtctc cgggagctgc
     6061 atgtgtcaga ggttttcacc gtcatcaccg aaacacgcga gttcgaaagg gcctcgtgat
     6121 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     6181 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     6241 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     6301 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     6361 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     6421 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     6481 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     6541 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     6601 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     6661 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     6721 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     6781 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     6841 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     6901 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     6961 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtggttc
     7021 acgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     7081 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     7141 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     7201 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     7261 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     7321 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     7381 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     7441 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     7501 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     7561 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     7621 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     7681 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     7741 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     7801 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     7861 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     7921 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     7981 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     8041 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     8101 tgagcgccca atacgcaaac cgcctctcac cgcgcgttgg ccgattcatt aatgcagctg
     8161 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaaacc gcctctcccc
     8221 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     8281 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     8341 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     8401 aacagctatg accatgatta cgccaaggtc gacttaaccc tagaaagata atcatattgt
     8461 gacgtacgtt aaagataatc atgcgtaaaa ttgacgcatg tgttttatcg gtctgtatat
     8521 cgaggtttat ttattaattt gaatagatat taagttttat tatatttaca cttacatact
     8581 aataataaat tcaacaaaca atttatttat gtttatttat ttattaaaaa aaaacaaaaa
     8641 ctcaaaattt cttctataaa gtaacaaaac ttttagcagt gaaaaaaatg ctttatttgt
     8701 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
     8761 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
     8821 agcaagtaaa acctctacaa atgtggtatg gctgattatg atcctctgga gattcatgcg
     8881 ccaggttttc tggtcatgca ccacgttcgt gggccctcag gaacctccac gtcagcggta
     8941 actgtgaagc ccagtcgctc gtaaaaagga agattccggg gagcactggt ttcgaggaac
     9001 gccggcacgc cagcccgttc cgcggcctca acaccaggaa gaacgacagc gcttcccaaa
     9061 cctttacctt ggtgatctgg gctgacaccg acagtggcaa gaaaccacgc aggctccttt
     9121 ggtctatgag gagccagcaa gccctccatt tgctgttgtg ccgccaacct actccctgac
     9181 agctcggcca tccgcggacc tatttccgcg aacactgcgc cggcttcgac gctttccgga
     9241 gtagtccaga ccgcaactgc ggcaccatcg tccgcaaccc aaactttacc aatgtccaga
     9301 cccacgcgcg taagaaacag ctcttgcaac tcagttaccc tctctatgtg tctgtccgga
     9361 tctacggtat ggcgggtcgc tggataatct gcaaatgctg cggcaagtgt tcggacagcc
     9421 cgtggtacat cgtcacgggt agcgagccta acagtgggct tatattccgt catggggccg
     9481 gggttctcct ccacgtcgcc ggcctgcttc agcaggctga agttggtggc gccgctgccc
     9541 ccggggagca tgtcaaggtc aaaatcgtca agagcgtcag caggcagcat atcaaggtca
     9601 aagtcgtcaa gggcatcggc tgggagcatg tctaagtcaa aatcgtcaag ggcgtcggtc
     9661 ggcccgccgc tttcgcactt tagctgtttc tccaggccac atatgattag ttccaggccg
     9721 aaaaggaagg caggttcggc tccctgccgg tcgaacagct caattgcttg tttcagaagt
     9781 gggggcatag aatcggtggt aggtgtctct ctttcctctt ttgctacttg atgctcctgt
     9841 tcctccaata cgcagcccag tgtaaagtgg cccacggcgg acagagcgta cagtgcgttc
     9901 tccagggaga agccttgctg acacaggaac gcgagctgat tttccagggt ttcgtactgt
     9961 ttctctgttg ggcgggtgcc gagatgcact ttagccccgt cacgatgtga gaggagagca
    10021 cagcggtatg acttggcgtt gttccgcaga aagtcttgcc atgactcgcc ttccaggggg
    10081 caggagtggg tatgatgcct gtccagcatc tcgattggca gggcatcgag cagggcccgc
    10141 ttgttcttca cgtgccagta cagggtaggc tgctcaactc ccagcttttg agcgagtttc
    10201 cttgtcgtca ggccttcgat accgactcca ttgagtaatt ccagagcaga gtttatgact
    10261 ttgctcttgt ccagtctaga catcttatcg tcatcgtctt tgtaatccat ggtggcggat
    10321 cccgcgtcac gacacctgtg ttctggcggc aaacccgttg cgaaaaagaa cgttcacggc
    10381 gactactgca cttatatacg gttctccccc accctcggga aaaaggcgga gccagtacac
    10441 gacatcactt tcccagttta ccccgcgcca ccttctctag gcaccggttc aattgccgac
    10501 ccctcccccc aacttctcgg gcactgtggg ccatgtgcgc tctgccccat ggtaatagcc
    10561 atgactaata cgtagatgta ctgccaagta ggaaagtgcc ataaggtcat gtactgggca
    10621 taatgccagg cgggccattt accgtcattg acgtcaatag ggggcgtact tggcatatga
    10681 tacacttgat gtactgccaa gtgggcagtt taccgtaaat actccaccca ttgacgtcaa
    10741 tggaaagtgc ctattggcgt tactatggga acatacgtca ttattgacgt caatgggcgg
    10801 gggtcgttgg gcggtcagcc aggcgggcca tttaccgtaa gttatgtaac gcggaactcc
    10861 atatatgggc tatgaactaa tgaccccgta attgattact attaataact agtcaataat
    10921 caatgtcata agctctaatc gcataatcga gctaagagcg attaatatgc ttagctagct
    10981 tagctaagct agatttctct attctcccac aatatatagc agctattagc ttagcagtcc
    11041 gaatacgatc gtccacgatc cataagcgta acacatttga gtagcgactg acgggcaccg
    11101 gagccactcg agtggaatt
//
