LOCUS       sequence-437876-        9614 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac transposon plasmid for doxycycline inducible expression of 
            HDAC6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9614)
  AUTHORS   Pierson Smela M, Adams J, Ma C, Breimann L, Widocki U, Shioda T, 
            Church GM
  TITLE     Induction of Meiosis from Human Pluripotent Stem Cells
  JOURNAL   bioRxiv 2024.05.31.596483
REFERENCE   2  (bases 1 to 9614)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.31.596483"
COMMENT     Sequence Label: pPB-cT3G-cERP2-HDAC6
FEATURES             Location/Qualifiers
     source          1..9614
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    17..35
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    53..71
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    89..107
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    125..143
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    161..179
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    197..215
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    233..251
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     278..302
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    386..410
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4068..4092)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4094..4120
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    4287..4421
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   4693..4727
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(4738..4755)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4738..4754)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4747..4769)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4963..4982)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5084..5106
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5145..5163)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5231..5335
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5336..5404,5405..6196)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5554..5573)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6367..6955
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6856..6875
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7109..7126
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7332..7353
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7368..7373,7374..7391,7392..7398)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7406..7422
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7411..7433
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7430..7446
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7430..7446
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   7472..7534
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    7710..7791
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(8508..8564)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(8574..9317)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(9318..9341)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      9341..9350
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(9370..9581)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 atcacctcga gtttactccc tatcagtgat agagaacgta tgaagagttt actccctatc
       61 agtgatagag aacgtatgca gactttactc cctatcagtg atagagaacg tataaggagt
      121 ttactcccta tcagtgatag agaacgtatg accagtttac tccctatcag tgatagagaa
      181 cgtatctaca gtttactccc tatcagtgat agagaacgta tatccagttt actccctatc
      241 agtgatagag aacgtataag cttgcctata taagcagagc tcgtttagtg aaccgtcaga
      301 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca
      361 gcctccgcgg tggcggcatc ggtacacaag tttgtacaaa aaagcaggct tagccaccat
      421 gacctcaacc ggccaggatt ccaccacaac caggcagcga agaagtaggc agaaccccca
      481 gtcgccccct caggactcca gtgtcacttc gaagcgaaat attaaaaagg gagccgttcc
      541 ccgctctatc cccaatctag cggaggtaaa gaagaaaggc aaaatgaaga agctcggcca
      601 agcaatggaa gaagacctaa tcgtgggact gcaagggatg gatctgaacc ttgaggctga
      661 agcactggct ggcactggct tggtgttgga tgagcagtta aatgaattcc attgcctctg
      721 ggatgacagc ttcccggaag gccctgagcg gctccatgcc atcaaggagc aactgatcca
      781 ggagggcctc ctagatcgct gcgtgtcctt tcaggcccgg tttgctgaaa aggaagagct
      841 gatgttggtt cacagcctag aatatattga tctgatggaa acaacccagt acatgaatga
      901 gggagaactc cgtgtcctag cagacaccta cgactcagtt tatctgcatc cgaactcata
      961 ctcctgtgcc tgcctggcct caggctctgt cctcaggctg gtggatgcgg tcctgggggc
     1021 tgagatccgg aatggcatgg ccatcattag gcctcctgga catcacgccc agcacagtct
     1081 tatggatggc tattgcatgt tcaaccacgt ggctgtggca gcccgctatg ctcaacagaa
     1141 acaccgcatc cggagggtcc ttatcgtaga ttgggatgtg caccacggtc aaggaacaca
     1201 gttcaccttc gaccaggacc ccagtgtcct ctatttctcc atccaccgct acgagcaggg
     1261 taggttctgg ccccacctga aggcctctaa ctggtccacc acaggtttcg gccaaggcca
     1321 aggatatacc atcaatgtgc cttggaacca ggtggggatg cgggatgctg actacattgc
     1381 tgctttcctg cacgtcctgc tgccagtcgc cctcgagttc cagcctcagc tggtcctggt
     1441 ggctgctgga tttgatgccc tgcaagggga ccccaagggt gagatggccg ccactccggc
     1501 agggttcgcc cagctaaccc acctgctcat gggtctggca ggaggcaagc tgatcctgtc
     1561 tctggagggt ggctacaacc tccgcgccct ggctgaaggc gtcagtgctt cgctccacac
     1621 ccttctggga gacccttgcc ccatgctgga gtcacctggt gccccctgcc ggagtgccca
     1681 ggcttcagtt tcctgtgctc tggaagccct tgagcccttc tgggaggttc ttgtgagatc
     1741 aactgagacc gtggagaggg acaacatgga ggaggacaat gtagaggaga gcgaggagga
     1801 aggaccctgg gagccccctg tgctcccaat cctgacatgg ccagtgctac agtctcgcac
     1861 agggctggtc tatgaccaaa atatgatgaa tcactgcaac ttgtgggaca gccaccaccc
     1921 tgaggtaccc cagcgcatct tgcggatcat gtgccgtctg gaggagctgg gccttgccgg
     1981 gcgctgcctc accctgacac cgcgccctgc cacagaggct gagctgctca cctgtcacag
     2041 tgctgagtac gtgggtcatc tccgggccac agagaaaatg aaaacccggg agctgcaccg
     2101 tgagagttcc aactttgact ccatctatat ctgccccagt accttcgcct gtgcacagct
     2161 tgccactggc gctgcctgcc gcctggtgga ggctgtgctc tcaggagagg ttctgaatgg
     2221 tgctgctgtg gtgcgtcccc caggacacca cgcagagcag gatgcagctt gcggtttttg
     2281 ctttttcaac tctgtggctg tggctgctcg ccatgcccag actatcagtg ggcatgccct
     2341 acggatcctg attgtggatt gggatgtcca ccacggtaat ggaactcagc acatgtttga
     2401 ggatgacccc agtgtgctat atgtgtccct gcaccgctat gatcatggca ccttcttccc
     2461 catgggggat gagggtgcca gcagccagat cggccgggct gcgggcacag gcttcaccgt
     2521 caacgtggca tggaacgggc cccgcatggg tgatgctgac tacctagctg cctggcatcg
     2581 cctggtgctt cccattgcct acgagtttaa cccagaactg gtgctggtct cagctggctt
     2641 tgatgctgca cggggggatc cgctgggggg ctgccaggtg tcacctgagg gttatgccca
     2701 cctcacccac ctgctgatgg gccttgccag tggccgcatt atccttatcc tagagggtgg
     2761 ctataacctg acatccatct cagagtccat ggctgcctgc actcgctccc tccttggaga
     2821 cccaccaccc ctgctgaccc tgccacggcc cccactatca ggggccctgg cctcaatcac
     2881 tgagaccatc caagtccatc gcagatactg gcgcagctta cgggtcatga aggtagaaga
     2941 cagagaagga ccctccagtt ctaagttggt caccaagaag gcaccccaac cagccaaacc
     3001 taggttagct gagcggatga ccacacgaga aaagaaggtt ctggaagcag gcatggggaa
     3061 agtcacctcg gcatcatttg gggaagagtc cactccaggc cagactaact cagagacagc
     3121 tgtggtggcc ctcactcagg accagccctc agaggcagcc acagggggag ccactctggc
     3181 ccagaccatt tctgaggcag ccattggggg agccatgctg ggccagacca cctcagagga
     3241 ggctgtcggg ggagccactc cggaccagac cacctcagag gagactgtgg gaggagccat
     3301 tctggaccag accacctcag aggatgctgt tgggggagcc acgctgggcc agactacctc
     3361 agaggaggct gtaggaggag ctacactggc ccagaccacc tcggaggcag ccatggaggg
     3421 agccacactg gaccagacta cgtcagagga ggctccaggg ggcaccgagc tgatccaaac
     3481 tcctctagcc tcgagcacag accaccagac ccccccaacc tcacctgtgc agggaactac
     3541 accccagata tctcccagta cactgattgg gagtctcagg accttggagc taggcagcga
     3601 atctcagggg gcctcagaat ctcaggcccc aggagaggag aacctactag gagaggcagc
     3661 tggaggtcag gacatggctg attcgatgct gatgcaggga tctaggggcc tcactgatca
     3721 ggccatattt tatgctgtga caccactgcc ctggtgtccc catttggtgg cagtatgccc
     3781 catacctgca gcaggcctag acgtgaccca accttgtggg gactgtggaa caatccaaga
     3841 gaattgggtg tgtctctctt gctatcaggt ctactgtggt cgttacatca atggccacat
     3901 gctccaacac catggaaatt ctggacaccc gctggtcctc agctacatcg acctgtcagc
     3961 ctggtgttac tactgtcagg cctatgtcca ccaccaggct ctcctagatg tgaagaacat
     4021 cgcccaccag aacaagtttg gggaggatat gccccaccca cactaatacc cagctttctt
     4081 gtacaaagtg gtatacccat acgatgttcc agattacgct tagtaatgag aaatactcag
     4141 aagatgtcac tcaccatagt ccgcagtgaa agataaatga tcgccttccc ttccagatcc
     4201 tagttttaga gctagaaata gcaagtaggc cactgagcaa gaactatgaa tgagatcttt
     4261 agacatattg cggccgcttt ctccgcgatc cagacatgat aagatacatt gatgagtttg
     4321 gacaaaccaa aactagaatg cagtgaaaaa aatgccttat ttgtgaaatt tgtgatgcta
     4381 ttgccttatt tgtaaccatt ataagctgca ataaacaagt tatctataac aagaaaatat
     4441 atatataata agttatcacg taagtagaac atgaaataac aatataatta tcgtatgagt
     4501 taaatcttaa aagtcacgta aaagataatc atgcgtcatt ttgactcacg cggtcgttat
     4561 agttcaaaat cagtgacact taccgcattg acaagcacgc ctcacgggag ctccaagcgg
     4621 cgactgagat gtcctaaatg cacagcgacg gattcgcgct atttagaaag agagagcaat
     4681 atttcaagaa tgcatgcgtc aattttacgc agactatctt tctagggtta agaattcact
     4741 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     4801 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     4861 ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt ttctccttac
     4921 gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc
     4981 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     5041 tctgctcccg gcatccgctt acagacaagc tgtgaccatg tctccgggag ctgcatgtgt
     5101 cagaggtttt caccgtcatc accgaaacac gcgagttcga aagggcctcg tgatacgcct
     5161 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     5221 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     5281 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     5341 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     5401 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     5461 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     5521 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     5581 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     5641 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     5701 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     5761 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     5821 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     5881 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     5941 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     6001 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg gttcacgcgg
     6061 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     6121 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     6181 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     6241 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     6301 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     6361 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     6421 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     6481 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     6541 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     6601 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     6661 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     6721 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     6781 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     6841 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     6901 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     6961 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     7021 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     7081 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggatgagcg
     7141 cccaatacgc aaaccgcctc tcaccgcgcg ttggccgatt cattaatgca gctggcacga
     7201 caggtttccc gactggaaag cgggcagtga gcgcaacgca aaccgcctct ccccgcgcgt
     7261 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     7321 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     7381 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     7441 tatgaccatg attacgccaa ggtcgactta accctagaaa gataatcata ttgtgacgta
     7501 cgttaaagat aatcatgcgt aaaattgacg catgtgtttt atcggtctgt atatcgaggt
     7561 ttatttatta atttgaatag atattaagtt ttattatatt tacacttaca tactaataat
     7621 aaattcaaca aacaatttat ttatgtttat ttatttatta aaaaaaaaca aaaactcaaa
     7681 atttcttcta taaagtaaca aaacttttag cagtgaaaaa aatgctttat ttgtgaaatt
     7741 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
     7801 aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag
     7861 taaaacctct acaaatgtgg tatggctgat tatgatcctc tggagattca tgcgccaggt
     7921 tttctggtca tgcaccacgt tcgtgggccc tcaggaacct ccacgtcagc ggtaactgtg
     7981 aagcccagtc gctcgtaaaa aggaagattc cggggagcac tggtttcgag gaacgccggc
     8041 acgccagccc gttccgcggc ctcaacacca ggaagaacga cagcgcttcc caaaccttta
     8101 ccttggtgat ctgggctgac accgacagtg gcaagaaacc acgcaggctc ctttggtcta
     8161 tgaggagcca gcaagccctc catttgctgt tgtgccgcca acctactccc tgacagctcg
     8221 gccatccgcg gacctatttc cgcgaacact gcgccggctt cgacgctttc cggagtagtc
     8281 cagaccgcaa ctgcggcacc atcgtccgca acccaaactt taccaatgtc cagacccacg
     8341 cgcgtaagaa acagctcttg caactcagtt accctctcta tgtgtctgtc cggatctacg
     8401 gtatggcggg tcgctggata atctgcaaat gctgcggcaa gtgttcggac agcccgtggt
     8461 acatcgtcac gggtagcgag cctaacagtg ggcttatatt ccgtcatggg gccggggttc
     8521 tcctccacgt cgccggcctg cttcagcagg ctgaagttgg tggcgccgct gcccccgggg
     8581 agcatgtcaa ggtcaaaatc gtcaagagcg tcagcaggca gcatatcaag gtcaaagtcg
     8641 tcaagggcat cggctgggag catgtctaag tcaaaatcgt caagggcgtc ggtcggcccg
     8701 ccgctttcgc actttagctg tttctccagg ccacatatga ttagttccag gccgaaaagg
     8761 aaggcaggtt cggctccctg ccggtcgaac agctcaattg cttgtttcag aagtgggggc
     8821 atagaatcgg tggtaggtgt ctctctttcc tcttttgcta cttgatgctc ctgttcctcc
     8881 aatacgcagc ccagtgtaaa gtggcccacg gcggacagag cgtacagtgc gttctccagg
     8941 gagaagcctt gctgacacag gaacgcgagc tgattttcca gggtttcgta ctgtttctct
     9001 gttgggcggg tgccgagatg cactttagcc ccgtcgcgat gtgagaggag agcacagcgg
     9061 tatgacttgg cgttgttccg cagaaagtct tgccatgact cgccttccag ggggcaggag
     9121 tgggtatgat gcctgtccag catctcgatt ggcagggcat cgagcagggc ccgcttgttc
     9181 ttcacgtgcc agtacagggt aggctgctca actcccagct tttgagcgag tttccttgtc
     9241 gtcaggcctt cgataccgac tccattgagt aattccagag cagagtttat gactttgctc
     9301 ttgtccagtc tagacatctt atcgtcatcg tctttgtaat ccatggtggc ggatcccgcg
     9361 tcacgacacc tgtgttctgg cggcaaaccc gttgcgaaaa agaacgttca cggcgactac
     9421 tgcacttata tacggttctc ccccaccctc gggaaaaagg cggagccagt acacgacatc
     9481 actttcccag tttaccccgc gccaccttct ctaggcaccg gttcaattgc cgacccctcc
     9541 ccccaacttc tcggggactg tgggcgatgt gcgctctgcc cactgacggg caccggagcc
     9601 actcgagtgg aatt
//
