LOCUS       sequence-437879-        9018 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses of GFP-tagged mTet1s_K852R in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9018)
  AUTHORS   Arroyo M, Hastert FD, Zhadan A, Schelter F, Zimbelmann S, Rausch C, 
            Ludwig AK, Carell T, Cardoso MC
  TITLE     Isoform-specific and ubiquitination dependent recruitment of Tet1 to
            replicating heterochromatin modulates methylcytosine oxidation.
  JOURNAL   Nat Commun. 2022 Sep 2;13(1):5173. doi: 10.1038/s41467-022-32799-8.
  PUBMED    36056023
REFERENCE   2  (bases 1 to 9018)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-09-2"; volume: "13(1):5173. doi"; pages: " 
            10.1038/s41467-022-32799-8"
COMMENT     Sequence Label: pGFP-mTet1s_K852R  pc3914
FEATURES             Location/Qualifiers
     source          1..9018
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        69..372
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        374..651
                     /label=chicken beta-actin promoter
     intron          652..1668
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1592..1614
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1676..1695
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1740..1749
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1746..1748,1749..1751,1752..2462)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1746..1748,1749..1751,1752..2462)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1791..1812)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2052..2071)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2399..2420
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2469..2489
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     misc_feature    4715..5177
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4771..4788)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4998..5017
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      5182..5191
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5188..5586
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    5744..5865
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5781..5800)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5835..5854
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5913..6048
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6154..6171)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6325..6913)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6405..6424)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7084..7875,7876..7944))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7707..7726
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7945..8049)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8117..8135
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8173..8195)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8295..8314
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(8381..8836)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8518..8539)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8730..8749
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8962..8984
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8976..8993
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8977..8993
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        9000..9018
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 acgcgtccta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
       61 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
      121 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
      181 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
      241 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
      301 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
      361 gctattacca tggtcgaggt gagccccacg ttctgcttca ctctccccat ctcccccccc
      421 tccccacccc caattttgta tttatttatt ttttaattat tttgtgcagc gatgggggcg
      481 gggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg gggcggggcg
      541 aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg
      601 gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc gggagtcgct
      661 gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc gccccggctc
      721 tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt
      781 aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga aagccttgag
      841 gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg cgtgtgtgtg
      901 tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg ctgcgggcgc
      961 ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg gggcggtgcc
     1021 ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg
     1081 gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca cccccctccc
     1141 cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg tggcgcgggg
     1201 ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc ggggccgcct
     1261 cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg cggctgtcga
     1321 ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc gcagggactt
     1381 cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac cccctctagc
     1441 gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga gggccttcgt
     1501 gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc gcggggggac
     1561 ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt gaccggcggc
     1621 tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct cctgggcaac
     1681 gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tcggatccac cggccggtcg
     1741 ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     1801 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     1861 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     1921 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca
     1981 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     2041 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     2101 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     2161 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga
     2221 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
     2281 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     2341 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
     2401 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     2461 agtccggaga aaacctatac ttccaaggaa gatctcgagg cgatcgcatg gaagctgcac
     2521 cctgtgactg tgatggaggt acacaaaaag aaaaaggccc atattataca caccttgggg
     2581 caggaccaag tgtggctgct gtcagggagc tcatggagac taggtttggc cagaagggga
     2641 aggcaatccg gattgagaag atagtgttca cggggaagga agggaagagc tctcagggct
     2701 gcccggtcgc caagtgggtg atcagaagaa gtggtcctga agagaagctt atttgtttgg
     2761 ttcgtgagcg tgtagaccat cactgttcga cggctgtgat agttgtcctt atcctgctgt
     2821 gggaaggtat ccctcgcctg atggctgacc gcctgtacaa agagctcact gagaacttga
     2881 ggtcctacag cggacatccc acagaccgaa gatgtaccct caacaaaaag cgtacctgca
     2941 cctgtcaagg catcgaccca aaaacctgcg gagcgtcctt ctcctttggc tgttcgtgga
     3001 gcatgtattt caacggctgt cggtttggga ggagtgaaaa ccccagaaaa ttcagacttg
     3061 ctccaaacta ccccttacat aactactata agagaattac tggaatgagt tctgaaggaa
     3121 gtgacgtgaa aaccgggtgg atcattccag accgcaagac cctcataagc agagaggaaa
     3181 aacagcttga aaagaattta caagaattgg ctacagtatt agctccactt tacaagcaga
     3241 tggctccagt tgcttatcaa aatcaggtgg aatatgaaga agttgctgga gactgtcgac
     3301 ttggaaatga agaggggcgt cctttctctg gtgtcacctg ttgcatggat ttttgtgccc
     3361 attctcacaa ggacattcac aacatgcaca acggaagcac cgtggtgtgt acgttgattc
     3421 gagcagatgg ccgtgacaca aattgtcccg aggatgaaca actccacgtc ctgccactat
     3481 accggcttgc agacactgat gaatttggct ccgtggaagg gatgaaggcc aaaatcaaat
     3541 ctggggccat ccaagtcaat gggccaacca ggaagaggcg actacgtttt actgagcctg
     3601 ttcctcgatg tgggaagagg gccaaaatga agcagaacca caataaatca ggtactgctg
     3661 gtctcagaag gaagaggatc tctgccagcc caaagggagc cccaggttca cacaacacta
     3721 agagcttttc atcagcctca tctacttctc acctagtgaa agacgaatct acagacttct
     3781 gtcccctgca ggcttcctcc gcagaaacat ctacctgtac gtacagtaaa acagcctcag
     3841 gtgggtttgc agaaacaagt agtattctcc actgcacaat gccttctgga gcacacagtg
     3901 gtgctaatgc agctgctggg gaatgtactg gaacggtgca gcctgccgag gtggctgctc
     3961 atcctcacca gtctcttccc acagccgatt ctcccgttca tgctgagcct ctcactagtc
     4021 catctgagca gctaacttct aaccagtcaa accagcagct ccctctcctc agcaattctc
     4081 agaaactggc ttcctgtcag gtggaagatg agcggcaccc tgaagcggat gagcctcagc
     4141 accccgagga cgataacttg cctcaacttg atgaattctg gtcagacagt gaggagatct
     4201 acgccgatcc ttcctttggt ggcgtggcga tagcacccat tcacggctcg gtgctcattg
     4261 agtgcgctcg gaaggagctt catgctacca cctctttgcg ctcccccaaa cgaggggtcc
     4321 cttttcgtgt gtcccttgta ttctaccagc acaaaagcct aaacaagcct aatcatggtt
     4381 ttgatatcaa caaaattaag tgtaaatgca aaaaagtaac gaaaaaaaag cccgcagacc
     4441 gggagtgtcc tgatgtatcc cccgaagcca atttatcaca ccaaattcct tctcgagttg
     4501 catcaacctt aacccgagac aatgttgtta ccgtgtcccc atactctctc actcatgttg
     4561 cgggacccta caatcgttgg gtctaagcgg ccgcagttaa cgaattccgc ccctcccccc
     4621 cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt
     4681 tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct
     4741 tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga
     4801 atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga
     4861 ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac
     4921 gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag
     4981 ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc
     5041 agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg
     5101 tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt
     5161 gaaaaacacg atgataagct tgccaccatg gccaagcctt tgtctcaaga agaatccacc
     5221 ctcattgaaa gagcaacggc tacaatcaac agcatcccca tctctgaaga ctacagcgtc
     5281 gccagcgcag ctctctctag cgacggccgc atcttcactg gtgtcaatgt atatcatttt
     5341 actgggggac cttgtgcaga actcgtggtg ctgggcactg ctgctgctgc ggcagctggc
     5401 aacctgactt gtatcgtcgc gatcggaaat gagaacaggg gcatcttgag cccctgcgga
     5461 cggtgccgac aggtgcttct cgatctgcat cctgggatca aagccatagt gaaggacagt
     5521 gatggacagc cgacggcagt tgggattcgt gaattgctgc cctctggtta tgtgtgggag
     5581 ggctaagcac ttcgtggccg aggagcagga ctgacacgtg ctacgagatt tcgattccac
     5641 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     5701 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc
     5761 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     5821 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc
     5881 gtcgacctcg aggagctttt tgcaaaagcc taggcctcca aaaaagcctc ttcactactt
     5941 ctggaatagc tcagaggccg aggcggcctc ggcctctgca taaataaaaa aaattagtca
     6001 gccatggggc ggagaatggg cggaactggg cggagttagg ggcgggatgg gcggagttag
     6061 gggcgggact atggttgctg actaattgct gcattaatga atcggccaac gcgcggggag
     6121 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     6181 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     6241 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     6301 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     6361 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     6421 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     6481 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     6541 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     6601 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     6661 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     6721 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     6781 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     6841 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     6901 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     6961 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     7021 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     7081 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     7141 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     7201 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     7261 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     7321 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     7381 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     7441 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     7501 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     7561 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     7621 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     7681 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     7741 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     7801 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     7861 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     7921 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     7981 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     8041 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
     8101 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga
     8161 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
     8221 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
     8281 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
     8341 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg aaattgtaag cgttaatatt
     8401 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     8461 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     8521 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     8581 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
     8641 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     8701 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     8761 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gctcaatgcg
     8821 ccgctacagg gcgcgtccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
     8881 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
     8941 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgaattgt
     9001 aatacgactc actatagg
//
