LOCUS       sequence-437883-         634 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  His-tagged human PHD2 catalytic domain with TEV cleavage site 
            between His and PHD2 for PHD2 purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Gurol KC, Jursa T, Cho EJ, Fast W, Dalby KN, Smith DR, Mukhopadhyay 
            S
  TITLE     PHD2 enzyme is an intracellular manganese sensor that initiates the 
            homeostatic response against elevated manganese.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 Jun 25;121(26):e2402538121. doi: 
            10.1073/pnas.2402538121. Epub 2024 Jun 21.
  PUBMED    38905240
REFERENCE   2  (bases 1 to 634)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-06-25"; volume: "121(26):e2402538121. 
            doi"; pages: " 10.1073/pnas.2402538121. Epub 2024 Jun 21"
COMMENT     Sequence Label: His-PHD2-181-426
FEATURES             Location/Qualifiers
     source          1..634
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             18..35
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             57..77
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 gcgacttctc gtactaccat caccatcacc atcacgatta cgatatccca acgaccgaaa
       61 acctgtattt tcagggcgcc atggatccca acgggcagac gaagcccctg ccggcgctga
      121 agctggcgct cgagtacatc gtgccgtgca tgaacaagca cggcatctgt gtggtggacg
      181 acttcctcgg caaggagacc ggacagcaga tcggcgacga ggtgcgcgcc ctgcacgaca
      241 ccgggaagtt cacggacggg cagctggtca gccagaagag tgactcgtcc aaggacatcc
      301 gaggcgataa gatcacctgg atcgagggca aggagcccgg ctgcgaaacc attgggctgc
      361 tcatgagcag catggacgac ctgatacgcc actgtaacgg gaagctgggc agctacaaaa
      421 tcaatggccg gacgaaagcc atggttgctt gttatccggg ctatggaacg ggttatgtac
      481 gtcatgtgga tttccgctgg acatgcatga tgtgtggatt gataacccat ctaaaaatag
      541 acaacaatcc ttttgcaaaa gggcttccgg gacctggaaa tggccaaaga gaaacaaaat
      601 actctccccc cctgcactcc ctgaggtgtt tgat
//
