LOCUS       sequence-437894-       16509 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ABE cloning vector, insertion of gRNA sequence via Golden Gate with 
            BsaI and T4 ligase, blue-white screening. Replicates in E.coli and 
            Agrobacterium, binary vector for Agrobacterium T-DNA delivery.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16509)
  AUTHORS   Tan YY, Liew YY, Lee RRQ, Castel B, Chan NM, Wan WL, Zhang Y, Hu D, 
            Chan P, Kim ST, Chae E
  TITLE     Generation of Inheritable A-to-G Transitions Using Adenine Base 
            Editing and NG-PAM Cas9 in Arabidopsis thaliana.
  JOURNAL   Mol Plant Microbe Interact. 2025 Feb;38(1):30-42. doi: 
            10.1094/MPMI-10-24-0127-TA. Epub 2025 Feb 25.
  PUBMED    39585742
REFERENCE   2  (bases 1 to 16509)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-10-24-0127-TA"; journalName: "Mol Plant Microbe 
            Interact"; date: "2025-02"; volume: "38"; issue: "1"; pages: "30-42"
COMMENT     Sequence Label: pECNUS3
FEATURES             Location/Qualifiers
     source          1..16509
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1200..1895
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSDMALK
                     LVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGHK"
     promoter        2362..2540
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        2394..2576
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     terminator      3303..3555
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4231..4251)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(11574..11897)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     primer_bind     11579..11598
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11792..11814
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11806..11823
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11807..11823
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    11824..11880
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(11893..11909)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11893..11909)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11906..11928)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11917..11933
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11941..11947,11948..11965,11966..11971))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11986..12007
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    12286..12310
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      12398..13108
                     /label=oriV
                     /note="incP origin of replication"
     promoter        13129..13233
                     /gene="bla"
                     /label=AmpR promoter
     CDS             13234..14028
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      14199..14787
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14688..14707
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             15123..16271
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    16417..16441
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 tgccgaattc ggatccggag ttctagaatg tcgcggaaca aattttaaaa ctaaatccta
       61 aatttttcta attttgttgc caatagtgga tatgtgggcc gtatagaagg aatctattga
      121 aggcccaaac ccatactgac gagcccaaag gttcgttttg cgttttatgt ttcggttcga
      181 tgccaacgcc acattctgag ctaggcaaaa aacaaacgtg tctttgaata gactcctctc
      241 gttaacacat gcagcggctg catggtgacg ccattaacac gtggcctaca attgcatgat
      301 gtctccattg acacgtgact tctcgtctcc tttcttaata tatctaacaa acactcctac
      361 ctcttccaaa atatatacac atctttttga tcaatctctc attcaaaatc tcattctctc
      421 tagtaaacaa gaacaaaaaa atggcggata cagctagagg aacccatcac gatatcatcg
      481 gcagagatca gtacccgatg atgggccgag atcgtgacca gtaccagatg tccggacgag
      541 gatctgacta ctccaagtct aggcagattg ctaaagctgc aactgctgtc acagctggtg
      601 gttccctcct tgttctctcc agccttaccc ttgttggaac tgtcatagct ttgactgttg
      661 caacacctct gctcgttatc ttcagcccaa tccttgtccc ggctctcatc acagttgcac
      721 tcctcatcac cggttttctt tcctctggag ggtttggcat tgccgctata accgttttct
      781 cttggattta caagtaagca cacatttatc atcttacttc ataattttgt gcaatatgtg
      841 catgcatgtg ttgagccagt agctttggat caattttttt ggtcgaataa caaatgtaac
      901 aataagaaat tgcaaattct agggaacatt tggttaacta aatacgaaat ttgacctagc
      961 tagcttgaat gtgtctgtgt atatcatcta tataggtaaa atgcttggta tgatacctat
     1021 tgattgtgaa taggtacgca acgggagagc acccacaggg atcagacaag ttggacagtg
     1081 caaggatgaa gttgggaagc aaagctcagg atctgaaaga cagagctcag tactacggac
     1141 agcaacatac tggtggggaa catgaccgtg accgtactcg tggtggccag cacactacta
     1201 tgagcgagct gattaaggag aacatgcaca tgaagctgta catggagggc accgtgaaca
     1261 accaccactt caagtgcaca tccgagggcg aaggcaagcc ctacgagggc acccagacca
     1321 tgagaatcaa ggtggtcgag ggcggccctc tccccttcgc cttcgacatc ctggctacca
     1381 gcttcatgta cggcagcaga accttcatca accacaccca gggcatcccc gacttcttta
     1441 agcagtcctt ccctgagggc ttcacatggg agagagtcac cacatacgaa gatgggggcg
     1501 tgctgaccgc tacccaggac accagcctcc aggacggctg cctcatctac aacgtcaaga
     1561 tcagaggggt gaacttccca tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg
     1621 aggccaacac cgagatgctg taccccgctg acggcggcct ggaaggcaga agcgacatgg
     1681 ccctgaagct cgtgggcggg ggccacctga tctgcaactt caagaccaca tacagatcca
     1741 agaaacccgc taagaacctc aagatgcccg gcgtctacta tgtggaccac agactggaaa
     1801 gaatcaagga ggccgacaaa gaaacctacg tcgagcagca cgaggtggct gtggccagat
     1861 actgcgacct ccctagcaaa ctggggcaca agtgagctta ccccactgat gtcatcgtca
     1921 tagtccaata actccaatgt cggggagtta gtttatgagg aataaagtgt ttagaatttg
     1981 atcaggggga gataataaaa gccgagtttg aatctttttg ttataagtaa tgtttatgtg
     2041 tgtttctata tgttgtcaaa tggtaccatg ttttttttcc tctctttttg taacttgcaa
     2101 gtgttgtgtt gtactttatt tggcttcttt gtaagttggt aacggtggtc tatatatgga
     2161 aaaggtcttg ttttgttaaa cttatgttag ttaactggat tcgtctttaa ccacaaaaag
     2221 ttttcaataa gctacaaatt tagacacgca agccgatgca gtcattagta catatattta
     2281 ttgcaagtga ttacatggca acccaaactt caaaaacagt aggttgctcc atttagtcgc
     2341 tgcaagaatt caagcttgga ggaaccgcaa cgttgaagga gccactcagc cgcgggtttc
     2401 tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc aaaagtcgcc
     2461 taaggtcact atcagctagc aaatatttct tgtcaaaatg ctccactgac gttccataaa
     2521 ttcccctcgg tatccaatta gagtctcata ttcactctca atccaaataa tctgcaccgg
     2581 atctggatcg tttcgcatgt ctcctgaaag aaggcctgct gatattagaa gggctactga
     2641 ggctgatatg cctgctgtgt gcactattgt gaatcattac attgagactt ctactgtgaa
     2701 tttcaggact gagcctcaag aaccacaaga gtggactgat gatcttgtga ggcttagaga
     2761 gaggtatcct tggcttgttg ctgaagtgga tggtgaagtg gctggacatt gagacttcta
     2821 ctgtgaattt caggactgag cctcaagaac cacaagagtg gactgatgat cttgtgaggc
     2881 ttagagagag gtatccttgg cttgttgctg aagtggatgg tgaagtggct ggtattgctt
     2941 atgctggtcc ttggaaggct aggaatgctt acgattggac tgctgagtct actgtttacg
     3001 tgtctcctag gcatcaaaga actggtcttg gatctactct ttacactcat cttttgaagt
     3061 ctttggaggc tcagggtttc aagtctgtgg ttgctgtgat tggacttcct aatgatcctt
     3121 ctgtgaggat gcatgaggct cttggttatg ctcctagggg aatgcttaga gctgctggtt
     3181 tcaaacatgg aaattggcat gatgtgggat tctggcagct tgatttctct cttcctgttc
     3241 ctcctagacc tgttcttcct gtgactgaga tttgagcggg actctggatc tagagtcaag
     3301 cagatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg
     3361 cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat
     3421 gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat
     3481 acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat
     3541 ctatgttact agatccgcta ctagaattcg agctcagcgt tgtcaatcaa ttggcaagtc
     3601 ataaaatgca ttaaaaaata ttttcatact caactacaaa tccatgagta taactataat
     3661 tataaagcaa tgattagaat ctgacaagga ttctggaaaa ttacataaag gaaagttcat
     3721 aaatgtctaa aacacaagag gacatacttg tattcagtaa catttgcagc ttttctaggt
     3781 ctgaaaatat atttgttgcc tagtgaataa gcataatggt acaactacaa gtgttttact
     3841 cctcatatta acttcggtca ttagaggcca cgatttgaca catttttact caaaacaaaa
     3901 tgtttgcata tctcttataa tttcaaattc aacacacaac aaataagaga aaaaacaaat
     3961 aatattaatt tgagaatgaa caaaaggacc atatcattca ttaactcttc tccatccatt
     4021 tccatttcac agttcgatag cgaaaaccga ataaaaaaca cagtaaatta caagcacaac
     4081 aaatggtaca agaaaaacag ttttcccaat gccataatac tcaaactcag taggattctg
     4141 gtgtgtgcgc aatgaaactg atgcattgaa cttgacgaac gttgtcgaaa ccgatgatac
     4201 gaacgaaagc aagcagcgtt agccacctcc aaccttcctc ttcttcttag gatcagccct
     4261 tgaatcacca ccgagctgtg agagatcgat cctagtctcg tagagtccag tgatagactg
     4321 atggatgagg gtagcatcga gcacttcttt ggtagacctg tacaccttcc tatcgatggt
     4381 tgtatcgaag tacttgaaag ccctaggagc accgaggttg gtaagggtga agagatggat
     4441 gatgttctct gcctgttccc tgataggctt atctctgtgc ttgttgtaag cagacaacac
     4501 cttatcgagg tttgcatcag cgaggatcac ccttttagag aactcagaga tctgctcgat
     4561 gatctcatcc aagtagtgct tgtgctgctc aacgaaaagt tgcttctgct cgttatcttc
     4621 tggagatccc ttcaacttct cgtagtgaga agcgaggtaa agaaagttaa cgtacttaga
     4681 tgggagagca agctcgtttc ccttttgaag gaacctagca gaagcgagca tcctctttct
     4741 accgttctcg agttcgaaga gtgagtactt tgggagcttg atgatgagat ccttcttaac
     4801 ctctttgtat cccttagcct cgaggaaatc gattgggttc ttctcgaaag atgacctttc
     4861 catgatagtg attccgagaa gttccttaac agacttgagc ttcttactct ttcccttctc
     4921 aaccttagcc acaacgagaa cagagtaagc cacggtagga gacacgaaac caccgtattt
     4981 cttagggtcc caatccttct tcctagcaat gagcttatca gagttcctct taggcctgat
     5041 agactcttta gagaatccac cggtctgcac ctcggttttc ttaacgatgt tcacctgtgg
     5101 catagagagc acctttctaa cggtagcgaa atcccttccc ttatcccaca cgatctcacc
     5161 tgtttcaccg tttgtctcga tgagtggcct ctttctgatc tcaccgttag cgagggtaat
     5221 ctcggtcttg aagaaattca tgatgttaga gtagaagaaa tacttagcgg tagcctttcc
     5281 gatctcttgc tcagacttag cgatcatctt cctcacatcg tacaccttgt aatcaccgta
     5341 cacgaactct gactcgagct taggatactt cttgatgaga gcggttccaa caacagcgtt
     5401 aaggtaagca tcgtgagcgt ggtggtagtt gttgatttcc ctcaccttgt agaattggaa
     5461 atcctttctg aaatcagaca cgagctttga cttgagggtg ataaccttca cttccctgat
     5521 caacttatcg ttctcatcgt acttggtgtt catcctagaa tcgaggatct gtgcaacgtg
     5581 cttagtgatc tgcctggttt ccacaagctg cctcttgatg aatcctgcct tatccaattc
     5641 agagagtcct cccctctcag ccttagtcaa gttatcgaac tttctctgag tgatgagctt
     5701 agcgttgagg agctgcctcc aatagttctt cattttcttc acaacctctt cacttggcac
     5761 gttatcactc ttacccctgt tcttatcaga cctggtgagc accttgttat cgatagaatc
     5821 atccttcaag aatgactgtg gcacgatatg atcaacatcg taatcagaga gcctgttgat
     5881 atccaactct tgatccacat acatatccct tccgttctgg aggtagtaga ggtagagctt
     5941 ctcattctgg agctgagtgt tctcaacagg gtgctctttg aggatctgag atccaagctc
     6001 tttgatacct tcctcgatcc tcttcatcct ttccctagag ttcttctgtc ccttctgagt
     6061 ggtctggttc tctctagcca tttcgatcac gatgttctca ggcttatgcc ttcccatcac
     6121 cttcaccaac tcatccacaa ccttcacagt ctggaggatt cccttcttga ttgcaggaga
     6181 tccagcgagg ttagcgatat gctcatggag actatcaccc tgtcctgaaa cctgagcctt
     6241 ctggatatcc tctttaaagg tgagagaatc atcgtggatg agctgcatga agtttctgtt
     6301 agcgaatcca tcagacttga ggaaatcaag gattgtcttt ccagactgct tatccctgat
     6361 tccgttaatg agctttcttg agagccttcc ccaaccagtg tatcttcttc tcttcaactg
     6421 cttcatcacc ttatcatcga agagatgagc gtaggtcttg agcctttctt caatcatctc
     6481 tctatcttca aagagggtga gggtaagaac gatatcctcc aagatatcct cgttttcctc
     6541 gttatccaag aaatccttat ccttaatgat cttgaggaga tcgtggtagg ttccgagaga
     6601 tgcgttgaac ctatcctcaa caccagaaat ctcaactgaa tcgaagcact cgattttctt
     6661 gaagtaatcc tctttgagct gcttcacggt cacctttctg ttggtcttga acaagagatc
     6721 aacgatagcc ttcttttgct cacctgacaa aaaagcaggc ttcctcattc cctcggtcac
     6781 gtacttaacc ttggtcaact cgttgtacac ggtgaagtac tcgtagagca aagagtgctt
     6841 agggagcacc ttctcgtttg gaaggttctt atcgaagttg gtcatcctct cgatgaaaga
     6901 ctgagcacta gcacccttat ccaccacctc ttcgaagttc caaggggtga tggtttcctc
     6961 agactttctg gtcatccaag cgaatcttga gtttcctcta gcgagaggtc ccacgtagta
     7021 agggattctg aaggtgagaa tcttctcaat cttttccctg ttatccttga ggaatgggta
     7081 gaaatcctct tgccttctaa ggatagcgtg caactctccg aggtggatct gatgagggat
     7141 agatccgtta tcgaaggtcc tctgctttct gagaagatcc tctctattga gcttcacgag
     7201 gagttcctcg gttccatcca tcttctcgag gataggcttg atgaacttgt agaactcttc
     7261 ttgagatgca ccaccatcga tgtaaccagc gtatccgttc ttagactgat cgaagaaaat
     7321 ctctttgtac ttctctggga gctgctgtct aacaagagcc ttgagaagtg tgagatcctg
     7381 gtggtgctca tcgtatctct tgatcataga agctgagagt ggagccttgg tgatctcggt
     7441 gttcactctg aggatatcac tgaggaggat agcatcagag aggttcttag cagcgaggaa
     7501 caaatcagcg tactgatctc cgatctgagc gaggaggtta tcgagatcat catcgtaggt
     7561 atcctttgag agctggagct ttgcatcctc agcgagatcg aagttagact tgaagttagg
     7621 ggtgagtccg agagagagag cgatcaagtt tccgaaaagt ccgttcttct tctcaccagg
     7681 gagctgagca atgaggttct caagccttct tgacttagag agcctagcag agaggatagc
     7741 cttagcatcc acacctgaag cgttgatagg gttctcttcg aaaagctggt tgtaggtctg
     7801 cacgagctgg atgaacaact tatccacatc agagttatca gggttgagat caccctcgat
     7861 gaggaagtgt cctctgaact tgatcatgtg agcgagagcg aggtagatga gcctgagatc
     7921 agccttatca gtagaatcaa cgagcttctt tctgaggtgg tagatagtag ggtacttctc
     7981 gtggtatgcc acctcatcaa cgatgtttcc gaagataggg tgcctctcgt gcttcttatc
     8041 ttcttccacg aggaatgact cttcgagcct gtggaagaat gaatcatcca ctttagccat
     8101 ctcgttagag aagatctctt ggaggtagca gatcctgttc tttcttctgg tgtaccttct
     8161 tctagcggtt ctcttgagtc tggtagcctc agcagtttca ccagaatcga agaggagagc
     8221 accgataagg tttttcttga tagagtgcct atcggtgttt ccgagaacct tgaacttctt
     8281 agatggcacc ttgtactcat cggtgatcac agcccatccc acagagttag ttccgatggc
     8341 gagtccgata gagtacttct tatccattct gccgccagag ctgccacccg acgattctgg
     8401 cgtcgcgctc tccgaggttc ccggtgtctc cgaaccagag gatccccctg acgagcctcc
     8461 agaatccgtg gaggactggg ccttcttctg agcattgaac acctgcctcg gcatgcgaaa
     8521 aaagtagcaa agcagagcgg cacactcgtc cgcaagaatg ccttctgtta tctcaactct
     8581 gtgattcatc ccggggtaat gaagcacgtc catcagagat cctgccgccc cggttttggc
     8641 gttgcgaact ccaaaaacca cgcgtccgat ccggctatga atcatagcac cagcacacat
     8701 aacgcaaggc tcgaacgtga catagagtgt tgcgtcaata agcctatagt tctgcataac
     8761 cagccctccc tgcctcaaag ccataatctc tgcgtgcgcc gtagggtcgt gcaatccgat
     8821 tgccctattc cagccttcgc caatcaccct attgttcaac accaggactg cgccaacagg
     8881 aacctcgcgt tcgtctctgg cccgcttagc cagagtcagg gcgtgtctca tccagtactc
     8941 atgggagaat tcaacctcgg aagatccgcc gctagaccca ccagacgact ctggtgtcgc
     9001 cgactccgac gtgcctggag tctcagaacc tgacgacccc ccggagctgc caccagaatc
     9061 cgtcgatgac tgtgccttct tctgcgcttt gatttcttgt ctccgcatcc ggaagaagtc
     9121 ggaaagcaga gccgcgcatt catccgccaa aataccctcg gtgatttcca ccctgtggtt
     9181 catgccagga tggtgcaaaa cgtccataag gctacctgct gcgcctgttt ttgcatccct
     9241 agcgccgaac acgacgcgcc caatgcggga atgaatcatt gcgcccgcgc acatgacaca
     9301 cggctcgaga gtcacataaa gagtagcgtc tatcaagcga tagttctgca tcaccagacc
     9361 cccctgtcta agagccataa tctcagcatg tgctgttgga tcgtgtctgc ctatcggccg
     9421 attccacccc tctccgataa cgcggttatt gtgcacaagc acagccccca ccggaacttc
     9481 ccgctcgtcc cacgctctct tggccaacgt caaagcgtgc cgcatccaat actcgtggga
     9541 aaactcaact tctgacattg gctgtggtga gagaaacaga gcgtgagctc aaatacacaa
     9601 gtacgaaatc agagttatga gagaggattt ggagaaagaa gatcacctga agatcgtttg
     9661 cgtcgcgcga atgagagaga cggcagagaa aacgagaagc tttaagagga cgaacaaaaa
     9721 ccctaatgtc actcctttta tatagatgag aaatttaggg tttgggctct aatggagctg
     9781 tctacatggg ctttcatttc tttatccagt aaagaaccgg tttaacaggt tccctggttt
     9841 atacgtggtt ttagccggtt aacttacgga atattatttt tatcttaagt tacacaaatt
     9901 acatcattaa tagtaaaaca aaattttatg gtgcaattta agtttttatg atgagtgaaa
     9961 ttcgaataat atacaaatat tttactgttt aggtttccct aaacctactt ttagatatgt
    10021 aaattaaaca tttttttttt tttggttaga accaagtcag tgatcataga actcaaaaag
    10081 ctggtgtttc aattggtaca agcattttta ttgcaagata taaaaattca gaaggacata
    10141 tccttgatgt atgtacatga aggaaccgat tttgactcca agctacaaag aagaagaaga
    10201 atgatataca aagaaaactg catatcctca ccatgaaatt gaaagctaga ggaaggggaa
    10261 taacagatac tctgcgtaga agagattcca tataaaccga taatatcctg agatagcttc
    10321 ctgcaataat caaatgtttc tacactccgt tagatataca ctccattgat gttccgtgat
    10381 aacgagttta tgtttgtaca aaagtatttt accttggtgt agtttgcttt ttctagtacc
    10441 catgtctacc aaagatcaac aacaaagata ttagagatta atgaatttgg cagtgtcaaa
    10501 tgcagaaact aaagcatata gttatgaagt tcttgtgcat tatctcaaaa gtatgtaaga
    10561 gatataagag tatctcataa tgctttacct ggaaaattaa gcctgaagcc aagatcacat
    10621 gtgcaggaat catcaagcta cctctaaaaa cctgttcatt gttaaaggat aagagggttg
    10681 gtcttttcaa agctgcatac tgaggaaagt aacaatatcg caacaaaaat aaattggtgg
    10741 cgatagttta tcaagcttct acatgcagta atggtgttcg tgctttaatt aaccgaaata
    10801 gttaagacgg aaatagatga attcacctga ggcatgtaga aagctagtga tatggctgaa
    10861 acataattta ctagcagaag tccagaaccg aggaatgcaa tgtttctcac tccaagtttt
    10921 gttgccaggg ttgatatttg gaacctattc aagatcaatc aaaggcatga ttaactaaac
    10981 atgcttgaac aaacacataa agacaatata tcaaacggag ctggttaaac attgtctttg
    11041 actgtcaaat ggattgagcc aaagagcgga aagaaaatgg ttatgcgtgc agttacttac
    11101 tttcgatctc cttcaacatc aggaaggtcc tttgtaatag caatgaccag tgcaaacagt
    11161 gtcacaaaag atgtgatgaa cgccacaggt gcactgcaaa tagcgaatca aaagttgagt
    11221 aatatatcta ctcacgatta tggggagctc cttacgaatt cccatgggga gcatcttcat
    11281 tcttaagata tgaagataat cttcaaaagg cccctgggaa tctgaaagaa gagaagcagg
    11341 cccatttata tgggaaagaa caatagtatt tcttatatag gcccatttaa gttgaaaaca
    11401 atcttcaaaa gtcccacatc gcttagataa gaaaacgaag ctgagtttat atacagctag
    11461 agtcgaagta gtgattgtga gaccgtcaca gcttgtctgt aagcggatgc cgggagcaga
    11521 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg
    11581 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
    11641 gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa
    11701 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca
    11761 aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc
    11821 agtgaattcg agctcggtac ccggggatcc tctagagtcg acctgcaggc atgcaagctt
    11881 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
    11941 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
    12001 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
    12061 gcggtctcag tttaagagct atgctggaaa cagcatagca agtttaaata aggctagtcc
    12121 gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt tttgcaaaat tttccagatc
    12181 gatttcttct tcctctgttc ttcggcgttc aatttctggg gtcgctcaga gaggatgcac
    12241 atgtgaccga gggacacgaa gtgatccgtt taaactatca gtgtttgaca ggatatattg
    12301 gcgggtaaac ctaagagaaa agagcgttta ttagaataat cggatattta aaagggcgtg
    12361 aaaaggttta tccgttcgtc catttgtatg tgccagccgc ctttgcgacg ctcaccgggc
    12421 tggttgccct cgccgctggg ctggcggccg tctatggccc tgcaaacgcg ccagaaacgc
    12481 cgtcgaagcc gtgtgcgaga caccgcggcc gccggcgttg tggatacctc gcggaaaact
    12541 tggccctcac tgacagatga ggggcggacg ttgacacttg aggggccgac tcacccggcg
    12601 cggcgttgac agatgagggg caggctcgat ttcggccggc gacgtggagc tggccagcct
    12661 cgcaaatcgg cgaaaacgcc tgattttacg cgagtttccc acagatgatg tggacaagcc
    12721 tggggataag tgccctgcgg tattgacact tgaggggcgc gactactgac agatgagggg
    12781 cgcgatcctt gacacttgag gggcagagtg ctgacagatg aggggcgcac ctattgacat
    12841 ttgaggggct gtccacaggc agaaaatcca gcatttgcaa gggtttccgc ccgtttttcg
    12901 gccaccgcta acctgtcttt taacctgctt ttaaaccaat atttataaac cttgttttta
    12961 accagggctg cgccctgtgc gcgtgaccgc gcacgccgaa ggggggtgcc cccccttctc
    13021 gaaccctccc ggcccgctaa cgcgggcctc ccatcccccc aggggctgcg cccctcggcc
    13081 gcgaacggcc tcaccccaaa aatggcagcg ctggccaatt cccgagtgcg cggaacccct
    13141 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
    13201 taaatgcttc aataatattg aaaaaggaag agtatggcta aaatgagaat atcaccggaa
    13261 ttgaaaaaac tgatcgaaaa ataccgctgc gtaaaagata cggaaggaat gtctcctgct
    13321 aaggtatata agctggtggg agaaaatgaa aacctatatt taaaaatgac ggacagccgg
    13381 tataaaggga ccacctatga tgtggaacgg gaaaaggaca tgatgctatg gctggaagga
    13441 aagctgcctg ttccaaaggt cctgcacttt gaacggcatg atggctggag caatctgctc
    13501 atgagtgagg ccgatggcgt cctttgctcg gaagagtatg aagatgaaca aagccctgaa
    13561 aagattatcg agctgtatgc ggagtgcatc aggctctttc actccatcga catatcggat
    13621 tgtccctata cgaatagctt agacagccgc ttagccgaat tggattactt actgaataac
    13681 gatctggccg atgtggattg cgaaaactgg gaagaggaca ctccatttaa agatccgcgc
    13741 gagctgtatg attttttaaa gacggaaaag cccgaagagg aacttgtctt ttcccacggc
    13801 gacctgggag acagcaacat ctttgtgaaa gatggcaaag taagtggctt tattgatctt
    13861 gggagaagcg gcagggcgga caagtggtat gacattgcct tctgcgtccg gtcgctcagg
    13921 gaggatatcg gggaagaaca gtatgtcgag ctattttttg acttactggg gatcaagcct
    13981 gattgggaga aaataaaata ttatatttta ctggatgaat tgttttagct gtcagaccaa
    14041 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
    14101 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
    14161 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
    14221 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
    14281 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
    14341 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
    14401 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
    14461 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
    14521 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
    14581 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
    14641 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
    14701 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
    14761 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
    14821 gcagatccta gatgtggcgc aacgatgccg gcgacaagca ggagcgcacc gacttcttcc
    14881 gcatcaagtg ttttggctct caggccgagg cccacggcaa gtatttgggc aaggggtcgc
    14941 tggtattcgt gcagggcaag attcggaata ccaagtacga gaaggacggc cagacggtct
    15001 acgggaccga cttcattgcc gataaggtgg attatctgga caccaaggca ccaggcgggt
    15061 caaatcagga ataagggcac attgccccgg cgtgagtcgg ggcaatcccg caaggagggt
    15121 gaatgaatcg gacgtttgac cggaaggcat acaggcaaga actgatcgac gcggggtttt
    15181 ccgccgagga tgccgaaacc atcgcaagcc gcaccgtcat gcgtgcgccc cgcgaaacct
    15241 tccagtccgt cggctcgatg gtccagcaag ctacggccaa gatcgagcgc gacagcgtgc
    15301 aactggctcc ccctgccctg cccgcgccat cggccgccgt ggagcgttcg cgtcgtcttg
    15361 aacaggaggc ggcaggtttg gcgaagtcga tgaccatcga cacgcgagga actatgacga
    15421 ccaagaagcg aaaaaccgcc ggcgaggacc tggcaaaaca ggtcagcgag gccaagcagg
    15481 ccgcgttgct gaaacacacg aagcagcaga tcaaggaaat gcagctttcc ttgttcgata
    15541 ttgcgccgtg gccggacacg atgcgagcga tgccaaacga cacggcccgc tctgccctgt
    15601 tcaccacgcg caacaagaaa atcccgcgcg aggcgctgca aaacaaggtc attttccacg
    15661 tcaacaagga cgtgaagatc acctacaccg gcgtcgagct gcgggccgac gatgacgaac
    15721 tggtgtggca gcaggtgttg gagtacgcga agcgcacccc tatcggcgag ccgatcacct
    15781 tcacgttcta cgagctttgc caggacctgg gctggtcgat caatggccgg tattacacga
    15841 aggccgagga atgcctgtcg cgcctacagg cgacggcgat gggcttcacg tccgaccgcg
    15901 ttgggcacct ggaatcggtg tcgctgctgc accgcttccg cgtcctggac cgtggcaaga
    15961 aaacgtcccg ttgccaggtc ctgatcgacg aggaaatcgt cgtgctgttt gctggcgacc
    16021 actacacgaa attcatatgg gagaagtacc gcaagctgtc gccgacggcc cgacggatgt
    16081 tcgactattt cagctcgcac cgggagccgt acccgctcaa gctggaaacc ttccgcctca
    16141 tgtgcggatc ggattccacc cgcgtgaaga agtggcgcga gcaggtcggc gaagcctgcg
    16201 aagagttgcg aggcagcggc ctggtggaac acgcctgggt caatgatgac ctggtgcatt
    16261 gcaaacgcta gggccttgtg gggtcagttc cggctggggg ttcagcagcc cctgctcgga
    16321 tctgttggac cggacagtag tcatggttga tgggctgcct gtatcgagtg gtgattttgt
    16381 gccgagctgc cggtcgggga gctgttggct ggctggtggc aggatatatt gtggtgtaaa
    16441 caaattgacg cttagacaac ttaataacac attgcggacg tttttaatgt actggggttg
    16501 aacactctg
//
