LOCUS       sequence-437901-       11170 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses of GFP-tagged mTet1s in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11170)
  AUTHORS   Arroyo M, Hastert FD, Zhadan A, Schelter F, Zimbelmann S, Rausch C, 
            Ludwig AK, Carell T, Cardoso MC
  TITLE     Isoform-specific and ubiquitination dependent recruitment of Tet1 to
            replicating heterochromatin modulates methylcytosine oxidation.
  JOURNAL   Nat Commun. 2022 Sep 2;13(1):5173. doi: 10.1038/s41467-022-32799-8.
  PUBMED    36056023
REFERENCE   2  (bases 1 to 11170)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-09-2"; volume: "13(1):5173. doi"; pages: " 
            10.1038/s41467-022-32799-8"
COMMENT     Sequence Label: pGFP-mTet1s  pc3901
FEATURES             Location/Qualifiers
     source          1..11170
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      866..1001
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    1049..1170
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1060..1079)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1114..1133
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(1328..1726)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     regulatory      1723..1732
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    1737..2310
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1897..1916)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     2126..2143
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(6577..6597)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(join(6604..7314,7315..7317,7318..7320))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(6604..7314,7315..7317,7318..7320))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6646..6667)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     6995..7014
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7254..7275
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      7317..7326
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(7371..7390)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          7398..8414
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(7452..7474)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(8415..8692)
                     /label=chicken beta-actin promoter
     enhancer        8694..8997
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        complement(9066..9084)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9091..9108)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9091..9107)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9100..9122)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9248..9703
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9335..9354)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9545..9566
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9770..9789)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9889..9911
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9949..9967)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10035..10139
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10140..10208,10209..11000)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10358..10377)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagcaatt agtcagcaac
      841 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
      901 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
      961 tgagctattc cagaagtagt gaagaggctt ttttggaggc ctaggctttt gcaaaaagct
     1021 cctcgaggtc gacggtatac agacatgata agatacattg atgagtttgg acaaaccaca
     1081 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     1141 gtaaccatta taagctgcaa taaacaagtt ggggtgggcg aagaactcca gcatgagatc
     1201 cccgcgctgg aggatcatcc agccggcgtc ccggaaaacg attccgaagc ccaacctttc
     1261 atagaaggcg gcggtggaat cgaaatctcg tagcacgtgt cagtcctgct cctcggccac
     1321 gaagtgctta gccctcccac acataaccag agggcagcaa ttcacgaatc ccaactgccg
     1381 tcggctgtcc atcactgtcc ttcactatgg ctttgatccc aggatgcaga tcgagaagca
     1441 cctgtcggca ccgtccgcag gggctcaaga tgcccctgtt ctcatttccg atcgcgacga
     1501 tacaagtcag gttgccagct gccgcagcag cagcagtgcc cagcaccacg agttctgcac
     1561 aaggtccccc agtaaaatga tatacattga caccagtgaa gatgcggccg tcgctagaga
     1621 gagctgcgct ggcgacgctg tagtcttcag agatggggat gctgttgatt gtagccgttg
     1681 ctctttcaat gagggtggat tcttcttgag acaaaggctt ggccatggtg gcaagcttat
     1741 catcgtgttt ttcaaaggaa aaccacgtcc ccgtggttcg gggggcctag acgttttttt
     1801 aacctcgact aaacacatgt aaagcatgtg caccgaggcc ccagatcaga tcccatacaa
     1861 tggggtacct tctgggcatc cttcagcccc ttgttgaata cgcttgagga gagccatttg
     1921 actctttcca caactatcca actcacaacg tggcactggg gttgtgccgc ctttgcaggt
     1981 gtatcttata cacgtggctt ttggccgcag aggcacctgt cgccaggtgg ggggttccgc
     2041 tgcctgcaaa gggtcgctac agacgttgtt tgtcttcaag aagcttccag aggaactgct
     2101 tccttcacga cattcaacag accttgcatt cctttggcga gaggggaaag acccctagga
     2161 atgctcgtca agaagacagg gccaggtttc cgggccctca cattgccaaa agacggcaat
     2221 atggtggaaa ataacatata gacaaacgca caccggcctt attccaagcg gcttcggcca
     2281 gtaacgttag ggggggggga gggagagggg gggggggggg gggcggaatt cgttaactgc
     2341 ggccgcttag acccaacgat tgtagggtcc cgcaacatga gtgagagagt atggggacac
     2401 ggtaacaaca ttgtctcggg ttaaggttga tgcaactcga gaaggaattt ggtgtgataa
     2461 attggcttcg ggggatacat caggacactc ccggtctgcg ggcttttttt tcgttacttt
     2521 tttgcattta cacttaattt tgttgatatc aaaaccatga ttaggcttgt ttaggctttt
     2581 gtgctggtag aatacaaggg acacacgaaa agggacccct cgtttggggg agcgcaaaga
     2641 ggtggtagca tgaagctcct tccgagcgca ctcaatgagc accgagccgt gaatgggtgc
     2701 tatcgccacg ccaccaaagg aaggatcggc gtagatctcc tcactgtctg accagaattc
     2761 atcaagttga ggcaagttat cgtcctcggg gtgctgaggc tcatccgctt cagggtgccg
     2821 ctcatcttcc acctgacagg aagccagttt ctgagaattg ctgaggagag ggagctgctg
     2881 gtttgactgg ttagaagtta gctgctcaga tggactagtg agaggctcag catgaacggg
     2941 agaatcggct gtgggaagag actggtgagg atgagcagcc acctcggcag gctgcaccgt
     3001 tccagtacat tccccagcag ctgcattagc accactgtgt gctccagaag gcattgtgca
     3061 gtggagaata ctacttgttt ctgcaaaccc acctgaggct gttttactgt acgtacaggt
     3121 agatgtttct gcggaggaag cctgcagggg acagaagtct gtagattcgt ctttcactag
     3181 gtgagaagta gatgaggctg atgaaaagct cttagtgttg tgtgaacctg gggctccctt
     3241 tgggctggca gagatcctct tccttctgag accagcagta cctgatttat tgtggttctg
     3301 cttcattttg gccctcttcc cacatcgagg aacaggctca gtaaaacgta gtcgcctctt
     3361 cctggttggc ccattgactt ggatggcccc agatttgatt ttggccttca tcccttccac
     3421 ggagccaaat tcatcagtgt ctgcaagccg gtatagtggc aggacgtgga gttgttcatc
     3481 ctcgggacaa tttgtgtcac ggccatctgc tcgaatcaac gtacacacca cggtgcttcc
     3541 gttgtgcatg ttgtgaatgt ccttgtgaga atgggcacaa aaatccatgc aacaggtgac
     3601 accagagaaa ggacgcccct cttcatttcc aagtcgacag tctccagcaa cttcttcata
     3661 ttccacctga ttttgataag caactggagc catctgcttg taaagtggag ctaatactgt
     3721 agccaattct tgtaaattct tttcaagctg tttttcctct ctgcttatga gggtcttgcg
     3781 gtctggaatg atccacccgg ttttcacgtc acttccttca gaactcattc cagtaattct
     3841 cttatagtag ttatgtaagg ggtagtttgg agcaagtctg aattttctgg ggttttcact
     3901 cctcccaaac ttacagccgt tgaaatacat gctccacgaa cagccaaagg agaaggacgc
     3961 tccgcaggtt tttgggtcga tgccttgaca ggtgcaggta cgctttttgt tgagggtaca
     4021 tcttcggtct gtgggatgtc cgctgtagga cctcaagttc tcagtgagct ctttgtacag
     4081 gcggtcagcc atcaggcgag ggataccttc ccacagcagg ataaggacaa ctatcacagc
     4141 cgtcgaacag tgatggtcta cacgctcacg aaccaaacaa ataagcttct cttcaggacc
     4201 acttcttctg atcacccact tggcgaccgg gcagccctga gagctcttcc cttccttccc
     4261 cgtgaacact atcttctcaa tccggattgc cttccccttc tggccaaacc tagtctccat
     4321 gagctccctg acagcagcca cacttggtcc tgccccaagg tgtgtataat atgggccttt
     4381 ttctttttgt gtacctccat cacagtcaca gggtgcagct tcctgttcct tcaatccagc
     4441 tatcaggttt tttgcaggct tgctaagtaa gtccatgatg ctctctttaa attctgactg
     4501 tgcactgtct gtggtgggac ccagtggtcc attgcttgat gtttgagctt cacgcttctc
     4561 cgtaacaact gtaattccat cgaaatgtaa cactttccct ttcctgagga ttgatgctgg
     4621 gtctggcaag agtgtgtcag gggaggtacc aggcaacgtc ggtggtggta tctgatgcac
     4681 caggttctct tgagcgccta gatgaaactg tgtttgggca gcaccagcca ttatattagc
     4741 acactggtta gatggtgggg actggggcag agggtgagct tcctgattga cactcaccat
     4801 gggctgtccc tgagaattat ctgccggctc tgcaaatgtc tgcccaccgg attctgtctt
     4861 gaattggcat gttggcagag aatctgatgg ttcaaccttc actttctcct ttgccaattc
     4921 agggtttctt gtgaatttta tctgattgat aggaggaaat aactcattat gttgtgaggg
     4981 tgttttactc tgagtcacgg gctttaatat ttggttaaag acaggaatat ttctcaacag
     5041 aacaggaaaa ctacgtggac tagactggcc aaacctttga aacttagatg acatccaaat
     5101 gtcatgcatt ttactatact ctatagctag ctgctcttgc ttcttctgat cattttcttg
     5161 agagcttcgg gctgggggtt tctttttccg cttgtttgcg tgtggggtcg ctcttgcctt
     5221 tttctgggcc ttctttggct ttgctgatgg tacactgggt ttagtttgtg tactagaggg
     5281 tggcttctga tgtaccatgc gtttctcctg actgtgtttt tgctttgtgt tctttgcaac
     5341 aaggctttct ggtgtacttt cagcattcct gacctcggcg ttgatgtgat taatcgtgga
     5401 tgcaagttgg gtcaattgtg ctgcgacatc ttcttcaatt tgactgtagg gtgcatcctg
     5461 gcatgatggg tcatgggaac tcaaattctg atcgcagctt agtacctgac tgcagttttc
     5521 aagattgtga ctttctatgg gcaatggatg tgaattctca aaaccatcaa agggagctct
     5581 cccaccagca gcgcctttct tctcagctgt aggtgaactt ggatatcctt gtgattttat
     5641 aagtgcttgg ttagtagcga aactatcagg tgacttgaat gttctgttct ggccattaaa
     5701 gacaacagtg tcatggtgaa ggccctttcc ttgaacgttt gggtcttgga ggtcttttct
     5761 caccgaatga aggatagcct tgcaactgtt aagcagacta gccgttttct gatgcgcttc
     5821 ttcatccttc tccggcttgg atggggagga gctctctgag ggcgcttctg agagctgagt
     5881 cagggcttct atggctacca cttgttccag cgttaatctc ctgtccttca ttagggatag
     5941 aagacttggc tgaaccggag agccatcttt gggttctttg ttgggtacct tctcaggagg
     6001 gtgaggtcga ctcccagcgc tgtgtgtatt agggtgatta ttaaagatgg tgggttctgc
     6061 accagacctg cagttcaagc caggcttctg gaagagcagg gtgttccttg gccccttgag
     6121 atgatcacac tcccccccgg aggtggtgac actcatggca tccttgcagt ttgagggatc
     6181 agtcagcaat ttagaggaat tatttcccaa cgccttgcta aattcctcat gattcaattt
     6241 gttgagcgga aggtgtgtgt cagtgggtaa acagtgtaca tttttcatgc cgttccttat
     6301 ggtctgggca aatgtcggag aagggttggt ttgctgcttg tcatcttcag agggctgggg
     6361 gttttccacc ccagttaaat tggcatcaca gcttcctttg acttgaccct ctgctaagtg
     6421 tgatttcttg tttggcgccc acttctccac ctcaattccc gtcatgccgc ctgcattctt
     6481 ccttacattt tcaaggggat gtgtgatcaa atccaacctt tgctcttctt ccccatgacc
     6541 acgtctactg cagtccatgc gatcgcctcg agatcttcct tggaagtata ggttttctcc
     6601 ggacttgtac agctcgtcca tgccgagagt gatcccggcg gcggtcacga actccagcag
     6661 gaccatgtga tcgcgcttct cgttggggtc tttgctcagg gcggactggg tgctcaggta
     6721 gtggttgtcg ggcagcagca cggggccgtc gccgatgggg gtgttctgct ggtagtggtc
     6781 ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc ttgaagttca ccttgatgcc
     6841 gttcttctgc ttgtcggcca tgatatagac gttgtggctg ttgtagttgt actccagctt
     6901 gtgccccagg atgttgccgt cctccttgaa gtcgatgccc ttcagctcga tgcggttcac
     6961 cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg tagttgccgt cgtccttgaa
     7021 gaagatggtg cgctcctgga cgtagccttc gggcatggcg gacttgaaga agtcgtgctg
     7081 cttcatgtgg tcggggtagc ggctgaagca ctgcacgccg taggtcaggg tggtcacgag
     7141 ggtgggccag ggcacgggca gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc
     7201 gtaggtggca tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt ttacgtcgcc
     7261 gtccagctcg accaggatgg gcaccacccc ggtgaacagc tcctcgccct tgctcaccat
     7321 ggtggcgacc ggccggtgga tccgaattct ttgccaaaat gatgagacag cacaataacc
     7381 agcacgttgc ccaggagctg taggaaaaag aagaaggcat gaacatggtt agcagaggct
     7441 ctagagccgc cggtcacacg ccagaagccg aaccccgccc tgccccgtcc cccccgaagg
     7501 cagccgtccc cccgcggaca gccccgaggc tggagaggga gaaggggacg gcggcgcggc
     7561 gacgcacgaa ggccctcccc gcccatttcc ttcctgccgg cgccgcaccg cttcgccccg
     7621 cgcccgctag agggggtgcg gcggcgcctc ccagatttcg gctccgcaca gatttgggac
     7681 aaaggaagtc cctgcgccct ctcgcacgat taccataaaa ggcaatggct gcggctcgcc
     7741 gcgcctcgac agccgccggc gctccggggg ccgccgcgcc cctcccccga gccctccccg
     7801 gcccgaggcg gccccgcccc gcccggcacc cccacctgcc gccacccccc gcccggcacg
     7861 gcgagccccg cgccacgccc cgtacggagc cccgcacccg aagccgggcc gtgctcagca
     7921 actcggggag gggggtgcag ggggggttgc agcccgaccg acgcgcccac accccctgct
     7981 caccccccca cgcacacacc ccgcacgcag cctttgttcc cctcgcagcc ccccccgcac
     8041 cgcggggcac cgcccccggc cgcgctcccc tcgcgcacac tgcggagcgc acaaagcccc
     8101 gcgccgcgcc cgcagcgctc acagccgccg ggcagcgcgg agccgcacgc ggcgctcccc
     8161 acgcacacac acacgcacgc accccccgag ccgctccccc cgcacaaagg gccctcccgg
     8221 agcccctcaa ggctttcacg cagccacaga aaagaaacaa gccgtcatta aaccaagcgc
     8281 taattacagc ccggaggaga agggccgtcc cgcccgctca cctgtgggag taacgcggtc
     8341 agtcagagcc ggggcgggcg gcgcgaggcg gcggcggagc ggggcacggg gcgaaggcag
     8401 cgcgcagcga ctcccgcccg ccgcgcgctt cgctttttat agggccgccg ccgccgccgc
     8461 ctcgccataa aaggaaactt tcggagcgcg ccgctctgat tggctgccgc cgcacctctc
     8521 cgcctcgccc cgccccgccc ctcgccccgc cccgccccgc ctggcgcgcg cccccccccc
     8581 ccccccgccc ccatcgctgc acaaaataat taaaaaataa ataaatacaa aattgggggt
     8641 ggggaggggg gggagatggg gagagtgaag cagaacgtgg ggctcacctc gaccatggta
     8701 atagcgatga ctaatacgta gatgtactgc caagtaggaa agtcccataa ggtcatgtac
     8761 tgggcataat gccaggcggg ccatttaccg tcattgacgt caataggggg cgtacttggc
     8821 atatgataca cttgatgtac tgccaagtgg gcagtttacc gtaaatactc cacccattga
     8881 cgtcaatgga aagtccctat tggcgttact atgggaacat acgtcattat tgacgtcaat
     8941 gggcgggggt cgttgggcgg tcagccaggc gggccattta ccgtaagtta tgtaacgcgg
     9001 aactccatat atgggctatg aactaatgac cccgtaattg attactatta ataactagga
     9061 cgcgtcctat agtgagtcgt attacaattc actggccgtc gttttacaac gtcgtgactg
     9121 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     9181 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     9241 cgaatggacg cgccctgtag cggcgcattg agcgcggcgg gtgtggtggt tacgcgcagc
     9301 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     9361 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     9421 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     9481 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     9541 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     9601 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     9661 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttcctgatg cggtattttc
     9721 tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct
     9781 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     9841 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     9901 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
     9961 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
    10021 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
    10081 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
    10141 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
    10201 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
    10261 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
    10321 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
    10381 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
    10441 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
    10501 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
    10561 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
    10621 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
    10681 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
    10741 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
    10801 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
    10861 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
    10921 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
    10981 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
    11041 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
    11101 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
    11161 aaggatcttc
//
