LOCUS       sequence-43791-e         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Larson DR, Zenklusen D, Wu B, Chao JA, Singer RH
  TITLE     Real-time observation of transcription initiation and elongation on 
            an endogenous yeast gene.
  JOURNAL   Science. 2011 Apr 22;332(6028):475-8.
  PUBMED    21512033
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2011-04-22"; volume: "332(6028)"; pages: "475-8"
COMMENT     Sequence Label: pDZ264 (pKAN tdtomato cyc loxP KAN loxP)
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(25..368)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     protein_bind    complement(423..456)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     terminator      complement(507..754)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     720..738
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     756..772
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     757..773
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aacgtgagtc ttttccttac ccatggttgt ttatgttcgg atgtgatgtg agaactgtat
       61 cctagcaaga ttttaaaagg aagtatatga aagaagaacc tcagtggcaa atcctaacct
      121 tttatatttc tctacagggg cgcggcgtgg ggacaattca acgcgtctgt gaggggagcg
      181 tttccctgct cgcaggtctg cagcgaggag ccgtaatttt tgcttcgcgc cgtgcggcca
      241 tcaaaatgta tggatgcaaa tgattataca tggggatgta tgggctaaat gtacgggcga
      301 cagtcacatc atgcccctga gctgcgcacg tcaagactgt caaggagggt attctgggcc
      361 tccatgtcgc tggccgggtg acccggcggg gacgaggcaa gctaaacaga tctctagacc
      421 taataacttc gtatagcata cattatacga agttatatta agggttgtcg acctgcagcg
      481 tacgaagctt cagctgggta ccggccgcaa attaaagcct tcgagcgtcc caaaaccttc
      541 tcaagcaagg ttttcagtat aatgttacat gcgtacacgc gtctgtacag aaaaaaaaga
      601 aaaatttgaa atataaataa cgttcttaat actaacataa ctataaaaaa ataaataggg
      661 acctagactt caggttgtct aactccttcc ttttcggtta gagcggatgt ggggggaggg
      721 cgtgaatgta agcgtgacat aactaattac atgactcgag gtcgacggta tcgataagct
      781 tgatatcgaa ttcgccctta tatattccgg acttgtacag ctcgtccatg ccgtacagga
      841 acaggtggtg gc
//
