LOCUS       sequence-437974-       10273 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To inducibly knockdown NEK6 expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10273)
  TITLE     Lee Lab shRNA knockdown vectors
REFERENCE   2  (bases 1 to 10273)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: LT3GEPIR-NEK6shRNA1
FEATURES             Location/Qualifiers
     source          1..10273
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    149..170
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(185..190,191..208,209..215)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    223..239
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     228..250
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     247..263
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     247..263
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     282..302
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        284..302
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        330..556
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             557..737
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    784..909
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1402..1635
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1820..1864
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2131..2248
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2264..2642
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    2272..2290
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2308..2326
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2344..2362
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2380..2398
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2416..2434
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2452..2470
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2488..2506
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2551..2575
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(2666..2668,2669..2671,2672..3385)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2711..2732)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2972..2991)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3319..3340
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     ncRNA           3579..3593
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        3773..4272
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3808..3831)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4205..4224
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4293..4892
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4293..4312)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4789..4809
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4953..5520
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4970..5522
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5114..5131)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5341..5360
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(5534..6151,6152..6157,6158..6193,6194..6196,6197..6232,
                     6233..6235,6236..6271,6272..6280)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    6326..6914
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6379..6399)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6797..6808)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7121..7354
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7426..7547
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7463..7482)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7517..7536
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7587..7722
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7742..7761)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7743..7761)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7771..7788)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7771..7787)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7780..7802)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7929..8384
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8016..8035)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8226..8247
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8410..8514
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8515..8583,8584..9375)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8733..8752)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9546..10134
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10035..10054
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
       61 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
      121 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
      181 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
      241 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
      301 gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct tgtagtcttg
      361 caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg
      421 ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct
      481 gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat ttaagtgcct
      541 agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
      601 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
      661 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
      721 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag
      781 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
      841 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
      901 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg
      961 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa
     1021 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga
     1081 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta
     1141 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac
     1201 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt
     1261 cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt
     1321 agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag
     1381 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag
     1441 cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat
     1501 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact
     1561 cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa
     1621 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt
     1681 gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg
     1741 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga
     1801 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag
     1861 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat
     1921 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt
     1981 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga
     2041 caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt
     2101 agtgaacgga tctcgacggt atcggttaac ttttaaaaga aaagggggga ttggggggta
     2161 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
     2221 aaaacaaatt acaaaaattc aaaattttat cgattctaga ttcgagttta ctccctatca
     2281 gtgatagaga acgtatgaag agtttactcc ctatcagtga tagagaacgt atgcagactt
     2341 tactccctat cagtgataga gaacgtataa ggagtttact ccctatcagt gatagagaac
     2401 gtatgaccag tttactccct atcagtgata gagaacgtat ctacagttta ctccctatca
     2461 gtgatagaga acgtatatcc agtttactcc ctatcagtga tagagaacgt ataagcttta
     2521 ggcgtgtacg gtgggcgcct ataaaagcag agctcgttta gtgaaccgtc agatcgcctg
     2581 gagcaattcc acaacacttt tgtcttatac caactttccg taccacttcc taccctcgta
     2641 aagtcgagct tgcgttggat ccaccatggt gagcaagggc gaggagctgt tcaccggggt
     2701 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtctgg
     2761 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     2821 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt
     2881 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     2941 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     3001 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     3061 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     3121 tatcatggcc gacaagcaga agaacggcat caaggcgaac ttcaagatcc gccacaacat
     3181 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     3241 ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc
     3301 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     3361 cggcatggac gagctgtaca agtaatgacg tacggcggcc gcctcgacta gggataacag
     3421 ggtaattgtt tgaatgaggc ttcagtactt tacagaatcg ttgcctgcac atcttggaaa
     3481 cacttgctgg gattacttcg acttcttaac ccaacagaag gctcgagaag gtatattgct
     3541 gttgacagtg agcgagaacc acccaaatat catcaatagt gaagccacag atgtattgat
     3601 gatatttggg tggttcgtgc ctactgcctc ggacttcaag gggtctagaa ttcgagcaat
     3661 tatcttgttt actaaaactg aataccttgc tatctctttg atacattttt acaaagctga
     3721 attaaaatgg tataaattaa atcacttttt tcaattgacg cgtaattcta ccgggtaggg
     3781 gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg
     3841 cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg
     3901 gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc
     3961 ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact
     4021 agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc
     4081 ggccaatagc agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc
     4141 gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc
     4201 ccggcattct gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct
     4261 ccgggccttt cgacctgcag cccaagctta ccatgaccga gtacaagccc acggtgcgcc
     4321 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact
     4381 accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc
     4441 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
     4501 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
     4561 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
     4621 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg
     4681 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
     4741 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
     4801 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca
     4861 cctggtgcat gacccgcaag cccggtgcct gacgcccgcc ccacgacccg cagcgcccga
     4921 ccgaaaggag cgcacgaccc catgcatcca attccgcccc ccccccctaa cgttactggc
     4981 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg
     5041 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct
     5101 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca
     5161 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg
     5221 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct
     5281 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa
     5341 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt
     5401 atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa
     5461 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa
     5521 tatggccaca accatgagta gactggacaa gagcaaagtc ataaacggag ctctggaatt
     5581 actcaatggt gtcggtatcg aaggcctgac gacaaggaaa ctcgctcaaa agctgggagt
     5641 tgagcagcct accctgtact ggcacgtgaa gaacaagcgg gccctgctcg atgccctgcc
     5701 aatcgagatg ctggacaggc atcataccca cttctgcccc ctggaaggcg agtcatggca
     5761 agactttctg cggaacaacg ccaagtcata ccgctgtgct ctcctctcac atcgcgacgg
     5821 ggctaaagtg catctcggca cccgcccaac agagaaacag tacgaaaccc tggaaaatca
     5881 gctcgcgttc ctgtgtcagc aaggcttctc cctggagaac gcactgtacg ctctgtccgc
     5941 cgtgggccac tttacactgg gctgcgtatt ggaggaacag gagcatcaag tagcaaaaga
     6001 ggaaagagag acacctacca ccgattctat gcccccactt ctgagacaag caattgagct
     6061 gttcgaccgg cagggagccg aacctgcctt ccttttcggc ctggaactaa tcatatgtgg
     6121 cctggagaaa cagctaaagt gcgaaagcgg cgggccgacc gacgcccttg acgattttga
     6181 cttagacatg ctcccagccg atgcccttga cgactttgac cttgatatgc tgcctgctga
     6241 cgctcttgac gattttgacc ttgacatgct ccccgggtaa tccggactcg acctgcagcc
     6301 aagcttatcg aaacgcgtct ggaacaatca acctctggat tacaaaattt gtgaaagatt
     6361 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     6421 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     6481 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     6541 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     6601 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     6661 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     6721 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     6781 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     6841 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     6901 ggccgcctcc ccgcctggaa ttaattctgc agtcgagacc tagaaaaaca tggagcaatc
     6961 acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag
     7021 gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca
     7081 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     7141 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     7201 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     7261 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     7321 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
     7381 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca
     7441 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     7501 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc
     7561 tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     7621 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     7681 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc
     7741 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg
     7801 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     7861 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     7921 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     7981 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     8041 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     8101 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     8161 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     8221 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     8281 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     8341 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg
     8401 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     8461 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     8521 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     8581 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     8641 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     8701 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     8761 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     8821 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     8881 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     8941 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     9001 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     9061 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     9121 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     9181 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     9241 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     9301 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     9361 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     9421 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     9481 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     9541 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     9601 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     9661 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     9721 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     9781 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     9841 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     9901 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     9961 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
    10021 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
    10081 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
    10141 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
    10201 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
    10261 gctcgccgca gcc
//
