LOCUS       sequence-43799-e         803 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Sanjana NE, Cong L, Zhou Y, Cunniff MM, Feng G, Zhang F
  TITLE     A transcription activator-like effector toolbox for genome 
            engineering.
  JOURNAL   Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431.
  PUBMED    22222791
REFERENCE   2  (bases 1 to 803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2011"; journalName: "Nat Protoc"; date: "2012-01-5- 
            5"; volume: "7"; issue: "1"; pages: "171-92"
COMMENT     Sequence Label: pNI_v2
FEATURES             Location/Qualifiers
     source          1..803
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(116..135)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     promoter        202..293
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cggcgttcct actctcgcat ggggagtccc cacactacca tcggcgctac ggcgtttcac
       61 ttctgagttc ggcatggggt caggtgggac caccgcgcta ctgccgccag gcaaacaagg
      121 ggtgttatga gccatattca ggtataaatg ggctcgcgat aatgttcaga attggttaat
      181 tggttgtaac actgacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
      241 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag aatatgagcc
      301 atattcaacg ggaaacgtcg aggccgcgat taaattccaa catggatgct gatttatatg
      361 ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg
      421 ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg
      481 ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgccactt ccgaccatca
      541 agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc cccggaaaaa
      601 cagcgttcca ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg
      661 cagtgttcct gcgccggttg cactcgattc ctgtttgtaa ttgtcctttt aacagcgatc
      721 gcgtatttcg cctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg
      781 attttgatga cgagcgtaat ggc
//
