LOCUS       sequence-437990-        7894 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac transposon plasmid for doxycycline inducible expression of 
            STRA8, with hygromycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7894)
  AUTHORS   Pierson Smela M, Adams J, Ma C, Breimann L, Widocki U, Shioda T, 
            Church GM
  TITLE     Induction of Meiosis from Human Pluripotent Stem Cells
  JOURNAL   bioRxiv 2024.05.31.596483
REFERENCE   2  (bases 1 to 7894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.31.596483"
COMMENT     Sequence Label: pPB-cT3G-cERH-STRA8
FEATURES             Location/Qualifiers
     source          1..7894
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    17..35
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    53..71
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    89..107
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    125..143
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    161..179
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    197..215
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    233..251
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     278..302
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    368..392
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      393..402
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(1539..1563)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1565..1591
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    1602..1736
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   2008..2042
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(2053..2070)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2053..2069)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2062..2084)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2278..2297)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2399..2421
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2460..2478)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2546..2650
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2651..2719,2720..3511)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2869..2888)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3682..4270
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4171..4190
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4424..4441
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4647..4668
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4683..4688,4689..4706,4707..4713)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4721..4737
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4726..4748
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4745..4761
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4745..4761
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   4787..4849
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    5025..5106
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(5223..6248)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVHRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(6249..6305)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(6315..7058)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(7059..7082)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      7082..7091
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(7111..7322)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     enhancer        7323..7702
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atcacctcga gtttactccc tatcagtgat agagaacgta tgaagagttt actccctatc
       61 agtgatagag aacgtatgca gactttactc cctatcagtg atagagaacg tataaggagt
      121 ttactcccta tcagtgatag agaacgtatg accagtttac tccctatcag tgatagagaa
      181 cgtatctaca gtttactccc tatcagtgat agagaacgta tatccagttt actccctatc
      241 agtgatagag aacgtataag cttgcctata taagcagagc tcgtttagtg aaccgtcaga
      301 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca
      361 gcctaccaca agtttgtaca aaaaagcagg ctgccaccat ggcaacccct gaagaaaaca
      421 gcaatcccca tgacagagca acaccccagc tgccagcaca gctgcaggag cttgagcatc
      481 gggtggcccg gagacggctg tcccaggccc gccaccgagc caccctggca gcgctcttca
      541 acaacctcag gaagacagtg tactctcagt ctgatctcat agcctcaaag tggcaggttc
      601 tgaataaggc aaagagtcat attccagaac tggagcaaac cctggataat ttgctgaagc
      661 tgaaagcatc cttcaacctg gaagatgggc atgcaagcag cttagaggag gtcaagaaag
      721 aatatgccag catgtattct ggaaatgaca gcctgctttc aaacagtttt cctcagaatg
      781 gttcctcccc ttggtgccca actgaggcag tcaggaagga tgctgaggag gaggaagatg
      841 aggaagagga agatcaagaa gaagaggagg aggaagaaga agaggaggag gaggaagagg
      901 aggaagagga agaggaggag gaggaagagg agaaaaaagt gatcttatac tccccaggaa
      961 ctttgtcgcc tgacctcatg gaatttgaac ggtatctcaa cttttacaaa cagacgatgg
     1021 accttctgac tggcagcggg atcattaccc cgcaggaggc ggcgctgccc atcgtctccg
     1081 cggccatctc ccacctgtgg cagaacctct cggaggagag gaaggccagc ctccggcagg
     1141 cctgggcgca gaagcaccgc ggccctgcga ccctggcgga ggcctgccga gagccggcct
     1201 gtgccgaggg cagcgtgaag gacagcggcg tggacagcca gggggccagc tgctcgctgg
     1261 tctccacgcc cgaggagatc ctttttgagg atgcctttga tgtggcaagc ttcctggaca
     1321 aaagtgaggt tccgagtaca tctagctcca gttcagtgct tgccagctgc aacccagaaa
     1381 acccagagga gaagtttcag ctctatatgc agatcatcaa cttttttaaa ggccttagct
     1441 gtgcaaacac tcaagtaaag caggaagcat cctttcccgt tgatgaagag atgatcatgt
     1501 tgcagtgcac agagaccttt gacgatgaag atttgtaaac ccagctttct tgtacaaagt
     1561 ggtataccca tacgatgttc cagattacgc tctttctccg cgatccagac atgataagat
     1621 acattgatga gtttggacaa accaaaacta gaatgcagtg aaaaaaatgc cttatttgtg
     1681 aaatttgtga tgctattgcc ttatttgtaa ccattataag ctgcaataaa caagttatct
     1741 ataacaagaa aatatatata taataagtta tcacgtaagt agaacatgaa ataacaatat
     1801 aattatcgta tgagttaaat cttaaaagtc acgtaaaaga taatcatgcg tcattttgac
     1861 tcacgcggtc gttatagttc aaaatcagtg acacttaccg cattgacaag cacgcctcac
     1921 gggagctcca agcggcgact gagatgtcct aaatgcacag cgacggattc gcgctattta
     1981 gaaagagaga gcaatatttc aagaatgcat gcgtcaattt tacgcagact atctttctag
     2041 ggttaagaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     2101 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     2161 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg
     2221 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac
     2281 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
     2341 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccatgtctcc
     2401 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacacgcgag ttcgaaaggg
     2461 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     2521 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     2581 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     2641 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     2701 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     2761 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     2821 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     2881 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     2941 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     3001 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     3061 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     3121 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     3181 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     3241 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     3301 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     3361 gcgtggttca cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     3421 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     3481 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     3541 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     3601 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     3661 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     3721 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     3781 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     3841 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     3901 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     3961 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     4021 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     4081 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     4141 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     4201 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     4261 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     4321 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     4381 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     4441 ggaagcggat gagcgcccaa tacgcaaacc gcctctcacc gcgcgttggc cgattcatta
     4501 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaaaccg
     4561 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     4621 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     4681 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     4741 cacacaggaa acagctatga ccatgattac gccaaggtcg acttaaccct agaaagataa
     4801 tcatattgtg acgtacgtta aagataatca tgcgtaaaat tgacgcatgt gttttatcgg
     4861 tctgtatatc gaggtttatt tattaatttg aatagatatt aagttttatt atatttacac
     4921 ttacatacta ataataaatt caacaaacaa tttatttatg tttatttatt tattaaaaaa
     4981 aaacaaaaac tcaaaatttc ttctataaag taacaaaact tttagcagtg aaaaaaatgc
     5041 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
     5101 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag
     5161 gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga tcctctggag
     5221 atctattcct ttgccctcgg acgagtgctg gggcgtcggt ttccactatc ggcgagtact
     5281 tctacacagc catcggtcca gacggccgcg cttctgcggg cgatttgtgt acgcccgaca
     5341 gtcccggctc cggatcggac gattgcgtcg catcgaccct gcgcccaagc tgcatcatcg
     5401 aaattgccgt caaccaagct ctgatagagt tggtcaagac caatgcggag catatacgcc
     5461 cggagccgcg gcgatcctgc aagctccgga tgcctccgct cgaagtagcg cgtctgctgc
     5521 tccatacaag ccaaccacgg cctccagaag aagatgttgg cgacctcgta ttgggaatcc
     5581 ccgaacatcg cctcgctcca gtcaatgacc gctgttatgc ggccattgtc cgtcaggaca
     5641 ttgttggagc cgaaatccgc gtgcacgagg tgccggactt cggggcagtc ctcggcccaa
     5701 agcatcagct catcgagagc ctgcgcgacg gacgcactga cggtgtcgtc catcacagtt
     5761 tgccagtgat acacatgggg atcagcaatc gcgcatatga aatcacgcca cgtagtgtat
     5821 tgaccgattc cttgcggtcc gaatgggccg aacccgctcg tctggctaag atcggccgca
     5881 gcgatcgcat ccatggcctc cgcgaccggc tgcagaacag cgggcagttc ggtttcaggc
     5941 aggtcttgca acgtgacacc ctgtgcacgg cgggagatgc aataggtcag gctctcgctg
     6001 aattccccaa tgtcaagcac ttccggaatc gggagcgcgg ccgatgcaaa gtgccgatga
     6061 acataacgat ctttgtagaa accatcggcg cagctattta cccgcaggac atatccacgc
     6121 cctcctacat cgaagctgaa agcacgagat tcttcgccct ccgagagctg catcaggtcg
     6181 gagacgctgt cgaacttttc gatcagaaac ttctcgacag acgtcgcggt gagttcaggc
     6241 tttttcatgg ggccggggtt ctcctccacg tcgccggcct gcttcagcag gctgaagttg
     6301 gtggcgccgc tgcccccggg gagcatgtca aggtcaaaat cgtcaagagc gtcagcaggc
     6361 agcatatcaa ggtcaaagtc gtcaagggca tcggctggga gcatgtctaa gtcaaaatcg
     6421 tcaagggcgt cggtcggccc gccgctttcg cactttagct gtttctccag gccacatatg
     6481 attagttcca ggccgaaaag gaaggcaggt tcggctccct gccggtcgaa cagctcaatt
     6541 gcttgtttca gaagtggggg catagaatcg gtggtaggtg tctctctttc ctcttttgct
     6601 acttgatgct cctgttcctc caatacgcag cccagtgtaa agtggcccac ggcggacaga
     6661 gcgtacagtg cgttctccag ggagaagcct tgctgacaca ggaacgcgag ctgattttcc
     6721 agggtttcgt actgtttctc tgttgggcgg gtgccgagat gcactttagc cccgtcacga
     6781 tgtgagagga gagcacagcg gtatgacttg gcgttgttcc gcagaaagtc ttgccatgac
     6841 tcgccttcca gggggcagga gtgggtatga tgcctgtcca gcatctcgat tggcagggca
     6901 tcgagcaggg cccgcttgtt cttcacgtgc cagtacaggg taggctgctc aactcccagc
     6961 ttttgagcga gtttccttgt cgtcaggcct tcgataccga ctccattgag taattccaga
     7021 gcagagttta tgactttgct cttgtccagt ctagacatct tatcgtcatc gtctttgtaa
     7081 tccatggtgg cggatcccgc gtcacgacac ctgtgttctg gcggcaaacc cgttgcgaaa
     7141 aagaacgttc acggcgacta ctgcacttat atacggttct cccccaccct cgggaaaaag
     7201 gcggagccag tacacgacat cactttccca gtttaccccg cgccaccttc tctaggcacc
     7261 ggttcaattg ccgacccctc cccccaactt ctcgggcact gtgggccatg tgcgctctgc
     7321 cccatggtaa tagccatgac taatacgtag atgtactgcc aagtaggaaa gtgccataag
     7381 gtcatgtact gggcataatg ccaggcgggc catttaccgt cattgacgtc aatagggggc
     7441 gtacttggca tatgatacac ttgatgtact gccaagtggg cagtttaccg taaatactcc
     7501 acccattgac gtcaatggaa agtgcctatt ggcgttacta tgggaacata cgtcattatt
     7561 gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg ggccatttac cgtaagttat
     7621 gtaacgcgga actccatata tgggctatga actaatgacc ccgtaattga ttactattaa
     7681 taactagtca ataatcaatg tcataagctc taatcgcata atcgagctaa gagcgattaa
     7741 tatgcttagc tagcttagct aagctagatt tctctattct cccacaatat atagcagcta
     7801 ttagcttagc agtccgaata cgatcgtcca cgatccataa gcgtaacaca tttgagtagc
     7861 gactgacggg caccggagcc actcgagtgg aatt
//
