LOCUS       sequence-438043-        8516 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FAP insert for C-terminally tagging genes of interest (optimized FAP
            for yeast expression + 2xMYC tag).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8516)
  AUTHORS   Oppenheimer KG, Hager NA, McAtee CK, Filiztekin E, Shang C, Warnick 
            JA, Bruchez MP, Brodsky JL, Prosser DC, Kwiatkowski AV, O'Donnell AF
  TITLE     Optimization of the fluorogen-activating protein tag for 
            quantitative protein trafficking and colocalization studies in S. 
            cerevisiae.
  JOURNAL   Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi: 10.1091/mbc.E24-04-0174. 
            Epub 2024 May 29.
  PUBMED    38809589
REFERENCE   2  (bases 1 to 8516)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2024-07-1"; volume: "35(7):mr5. doi"; pages: " 
            10.1091/mbc.E24-04-0174. Epub 2024 May 29"
COMMENT     Sequence Label: pRS415-ADH1pr-CFAPoptim
FEATURES             Location/Qualifiers
     source          1..8516
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(167..186)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     286..308
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(346..364)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    406..909
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        946..1050
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1051..1119,1120..1911)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1269..1288)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2082..2670
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2571..2590
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2824..2841
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2958..2979
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2994..2999,3000..3017,3018..3024)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3032..3048
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3037..3059
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3056..3072
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3056..3072
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3091..3111
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3093..3111
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3880..4584
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     primer_bind     4598..4614
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4598..4614
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(4648..4664)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4649..4665)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             4688..5407
                     /codon_start=1
                     /product="fluorogen-activating protein composed of a dimer 
                     of the L5-MG single-chain antibody fragment (Szent-Gyorgyi 
                     et al., 2008)"
                     /label=FAP-alpha-2
                     /note="binds malachite green derivatives to produce red 
                     fluorescence"
                     /translation="QAVVTQEPSVTVSPGGTVILTCGSGTGAVTSGHYANWFQQKPGQA
                     PRALIFDTDKKYSWTPGRFSGSLLGAKAALTISDAQPEDEAEYYCSLSDVDGYLFGGGT
                     QLTVLSGGGGSGGGGSGGGGSGGGGSQAVVTQEPSVTVSPGGTVILTCGSGTGAVTSGH
                     YANWFQQKPGQAPRALIFDTDKKYSWTPGRFSGSLLGAKAALTISDAQPEDEAEYYCSL
                     SDVDGYLFGGGTQLTVLS"
     CDS             5408..5437
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5438..5467
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      5480..5727
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(5496..5514)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(5745..5764)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5746..5764)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5774..5791)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5774..5790)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5783..5805)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5931..6386
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6018..6037)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6228..6249
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6687..7094
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             7095..8189
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
ORIGIN
        1 aaaaaaaaaa aatacaacaa ataaaaaaca ctcaatgacc tgaccatttg atggagttta
       61 agtcaatacc ttcttgaacc atttcccata atggtgaaag ttccctcaag aattttactc
      121 tgtcagaaac ggccttaacg acgtagtcga tatggtgcac tctcagtaca atctgctctg
      181 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
      241 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
      301 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
      361 tatttttata ggttaatgtc atgataataa tggtttctta ggacggatcg cttgcctgta
      421 acttacacgc gcctcgtatc ttttaatgat ggaataattt gggaatttac tctgtgttta
      481 tttattttta tgttttgtat ttggatttta gaaagtaaat aaagaaggta gaagagttac
      541 ggaatgaaga aaaaaaaata aacaaaggtt taaaaaattt caacaaaaag cgtactttac
      601 atatatattt attagacaag aaaagcagat taaatagata tacattcgat taacgataag
      661 taaaatgtaa aatcacagga ttttcgtgtg tggtcttcta cacagacaag atgaaacaat
      721 tcggcattaa tacctgagag caggaagagc aagataaaag gtagtatttg ttggcgatcc
      781 ccctagagtc ttttacatct tcggaaaaca aaaactattt tttctttaat ttcttttttt
      841 actttctatt tttaatttat atatttatat taaaaaattt aaattataat tatttttata
      901 gcacgtgatg aaaaggaccc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
      961 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     1021 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     1081 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     1141 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     1201 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     1261 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     1321 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     1381 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     1441 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     1501 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     1561 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     1621 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     1681 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     1741 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     1801 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     1861 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     1921 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     1981 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     2041 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     2101 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     2161 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     2221 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     2281 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     2341 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     2401 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     2461 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     2521 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     2581 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     2641 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     2701 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     2761 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     2821 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     2881 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     2941 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     3001 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     3061 aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag
     3121 ctggagctcg ccgggatcga agaaatgatg gtaaatgaaa taggaaatca aggagcatga
     3181 aggcaaaaga caaatataag ggtcgaacga aaaataaagt gaaaagtgtt gatatgatgt
     3241 atttggcttt gcggcgccga aaaaacgagt ttacgcaatt gcacaatcat gctgactctg
     3301 tggcggaccc gcgctcttgc cggcccggcg ataacgctgg gcgtgaggct gtgcccggcg
     3361 gagttttttg cgcctgcatt ttccaaggtt taccctgcgc taaggggcga gattggagaa
     3421 gcaataagaa tgccggttgg ggttgcgatg atgacgacca cgacaactgg tgtcattatt
     3481 taagttgccg aaagaacctg agtgcatttg caacatgagt atactagaag aatgagccaa
     3541 gacttgcgag acgcgagttt gccggtggtg cgaacaatag agcgaccatg accttgaagg
     3601 tgagacgcgc ataaccgcta gagtactttg aagaggaaac agcaataggg ttgctaccag
     3661 tataaataga caggtacata caacactgga aatggttgtc tgtttgagta cgctttcaat
     3721 tcatttgggt gtgcacttta ttatgttaca atatggaagg gaactttaca cttctcctat
     3781 gcacatatat taattaaagt ccaatgctag tagagaaggg gggtaacacc cctccgcgct
     3841 cttttccgat ttttttctaa accgtggaat atttcggata tccttttgtt gtttccgggt
     3901 gtacaatatg gacttcctct tttctggcaa ccaaacccat acatcgggat tcctataata
     3961 ccttcgttgg tctccctaac atgtaggtgg cggaggggag atatacaata gaacagatac
     4021 cagacaagac ataatgggct aaacaagact acaccaatta cactgcctca ttgatggtgg
     4081 tacataacga actaatactg tagccctaga cttgatagcc atcatcatat cgaagtttca
     4141 ctaccctttt tccatttgcc atctattgaa gtaataatag gcgcatgcaa cttcttttct
     4201 ttttttttct tttctctctc ccccgttgtt gtctcaccat atccgcaatg acaaaaaaat
     4261 gatggaagac actaaaggaa aaaattaacg acaaagacag caccaacaga tgtcgttgtt
     4321 ccagagctga tgaggggtat ctcgaagcac acgaaacttt ttccttcctt cattcacgca
     4381 cactactctc taatgagcaa cggtatacgg ccttccttcc agttacttga atttgaaata
     4441 aaaaaaagtt tgctgtcttg ctatcaagta taaatagacc tgcaattatt aatcttttgt
     4501 ttcctcgtca ttgttctcgt tccctttctt ccttgtttct ttttctgcac aatatttcaa
     4561 gctataccaa gcatacaatc aactccaagc tggccgctct agaactagtg gatcccccgg
     4621 gctgcaggaa ttcgatatca agcttatcga taccgtcgac ctcgacgcgg gtgcgggtgc
     4681 gggtatgcag gccgtcgtta cccaagaacc tagtgttacc gttagcccag gtggtactgt
     4741 tatacttact tgtggaagtg gtacgggtgc cgtcacatct ggtcattatg caaattggtt
     4801 tcaacaaaaa ccaggacaag ctccaagagc tttgattttt gatactgata agaagtattc
     4861 ttggacccca ggtagatttt ctggatcttt gctgggagca aaggcagctt tgacaatatc
     4921 agatgctcag cctgaggacg aagccgagta ttactgttct cttagcgacg tggatggcta
     4981 cttgtttggc ggtggaacac aactgacggt tctgtccggt ggtggcggct ctggtggcgg
     5041 tggcagcggc ggtggtggtt ccggaggcgg cggttctcag gctgtggtga ctcaggagcc
     5101 gtcagtgact gtgtccccag gagggacagt cattctcact tgtggctccg gcactggagc
     5161 tgtcaccagt ggtcattatg ccaactggtt ccagcagaag cctggccaag cccccagggc
     5221 acttatattt gacaccgaca agaagtattc ctggacccct ggccgattct caggctccct
     5281 ccttggggcc aaggctgccc tgaccatctc ggatgcgcag cctgaagatg aggctgagta
     5341 ttactgttcg ctctccgacg ttgacggtta tctgttcgga ggaggcaccc agctgaccgt
     5401 cctctccgag cagaagctga tcagcgagga ggacctggag cagaagctga tcagcgagga
     5461 ggacctgtaa cgcctcgagt catgtaatta gttatgtcac gcttacattc acgccctccc
     5521 cccacatccg ctctaaccga aaaggaagga gttagacaac ctgaagtcta ggtccctatt
     5581 tattttttta tagttatgtt agtattaaga acgttattta tatttcaaat ttttcttttt
     5641 tttctgtaca gacgcgtgta cgcatgtaac attatactga aaaccttgct tgagaaggtt
     5701 ttgggacgct cgaaggcttt aatttgcggc cggtacccaa ttcgccctat agtgagtcgt
     5761 attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     5821 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     5881 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg acgcgccctg
     5941 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     6001 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     6061 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     6121 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     6181 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     6241 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     6301 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     6361 taacaaaata ttaacgctta caatttcctg atgcggtatt ttctccttac gcatctgtgc
     6421 ggtatttcac accgcatatc gaccggtcga ggagaacttc tagtatatct acatacctaa
     6481 tattattgcc ttattaaaaa tggaatccca acaattacat caaaatccac attctcttca
     6541 aaatcaattg tcctgtactt ccttgttcat gtgtgttcaa aaacgttata tttataggat
     6601 aattatactc tatttctcaa caagtaattg gttgtttggc cgagcggtct aaggcgcctg
     6661 attcaagaaa tatcttgacc gcagttaact gtgggaatac tcaggtatcg taagatgcaa
     6721 gagttcgaat ctcttagcaa ccattatttt tttcctcaac ataacgagaa cacacagggg
     6781 cgctatcgca cagaatcaaa ttcgatgact ggaaattttt tgttaatttc agaggtcgcc
     6841 tgacgcatat acctttttca actgaaaaat tgggagaaaa aggaaaggtg agagcgccgg
     6901 aaccggcttt tcatatagaa tagagaagcg ttcatgacta aatgcttgca tcacaatact
     6961 tgaagttgac aatattattt aaggacctat tgttttttcc aataggtggt tagcaatcgt
     7021 cttactttct aacttttctt accttttaca tttcagcaat atatatatat atatttcaag
     7081 gatataccat tctaatgtct gcccctaaga agatcgtcgt tttgccaggt gaccacgttg
     7141 gtcaagaaat cacagccgaa gccattaagg ttcttaaagc tatttctgat gttcgttcca
     7201 atgtcaagtt cgatttcgaa aatcatttaa ttggtggtgc tgctatcgat gctacaggtg
     7261 ttccacttcc agatgaggcg ctggaagcct ccaagaaggc tgatgccgtt ttgttaggtg
     7321 ctgtgggtgg tcctaaatgg ggtaccggta gtgttagacc tgaacaaggt ttactaaaaa
     7381 tccgtaaaga acttcaattg tacgccaact taagaccatg taactttgca tccgactctc
     7441 ttttagactt atctccaatc aagccacaat ttgctaaagg tactgacttc gttgttgtca
     7501 gagaattagt gggaggtatt tactttggta agagaaagga agacgatggt gatggtgtcg
     7561 cttgggatag tgaacaatac accgttccag aagtgcaaag aatcacaaga atggccgctt
     7621 tcatggccct acaacatgag ccaccattgc ctatttggtc cttggataaa gctaatgttt
     7681 tggcctcttc aagattatgg agaaaaactg tggaggaaac catcaagaac gaattcccta
     7741 cattgaaggt tcaacatcaa ttgattgatt ctgccgccat gatcctagtt aagaacccaa
     7801 cccacctaaa tggtattata atcaccagca acatgtttgg tgatatcatc tccgatgaag
     7861 cctccgttat cccaggttcc ttgggtttgt tgccatctgc gtccttggcc tctttgccag
     7921 acaagaacac cgcatttggt ttgtacgaac catgccacgg ttctgctcca gatttgccaa
     7981 agaataaggt caaccctatc gccactatct tgtctgctgc aatgatgttg aaattgtcat
     8041 tgaacttgcc tgaagaaggt aaggccattg aagatgcagt taaaaaggtt ttggatgcag
     8101 gtatcagaac tggtgattta ggtggttcca acagtaccac cgaagtcggt gatgctgtcg
     8161 ccgaagaagt taagaaaatc cttgcttaaa aagattctct ttttttatga tatttgtaca
     8221 taaactttat aaatgaaatt cataatagaa acgacacgaa attacaaaat ggaatatgtt
     8281 catagggtag acgaaactat atacgcaatc tacatacatt tatcaagaag gagaaaaagg
     8341 aggatgtaaa ggaatacagg taagcaaatt gatactaatg gctcaacgtg ataaggaaaa
     8401 agaattgcac tttaacatta atattgacaa ggaggagggc accacacaaa aagttaggtg
     8461 taacagaaaa tcatgaaact atgattccta atttatatat tggaggattt tctcta
//
