LOCUS       sequence-438067-        8744 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mStayGold fused to Lamp1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8744)
  TITLE     Janelia Integrative Imaging
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Lamp1_mStayGold
FEATURES             Location/Qualifiers
     source          1..8744
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      3..12
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5..88,89..1255)
                     /codon_start=1
                     /product="human lysosome-associated membrane glycoprotein 
                     1"
                     /label=LAMP1
                     /translation="MAAPGSARRPLLLLLLLLLLGLMHCASAAMFMVKNGNGTACIMAN
                     FSAAFSVNYDTKSGPKNMTFDLPSDATVVLNRSSCGKENTSDPSLVIAFGRGHTLTLNF
                     TRNATRYSVQLMSFVYNLSDTHLFPNASSKEIKTVESITDIRADIDKKYRCVSGTQVHM
                     NNVTVTLHDATIQAYLSNSSFSRGETRCEQDRPSPTTAPPAPPSPSPSPVPKSPSVDKY
                     NVSGTNGTCLLASMGLQLNLTYERKDNTTVTRLLNINPNKTSASGSCGAHLVTLELHSE
                     GTTVLLFQFGMNASSSRFFLQGIQLNTILPDARDPAFKAANGSLRALQATVGNSYKCNA
                     EEHVRVTKAFSVNIFKVWVQAFKVEGGQFGSVEECLLDENSMLIPIAVGGALAGLVLIV
                     LIAYLVGRKRSHAGYQTI"
     CDS             1283..1957
                     /codon_start=1
                     /product="bright and highly photostable monomeric green 
                     fluorescent protein (Ando et al., 2023)"
                     /label=mStayGold(J)
                     /note="includes multiple mutations plus an N-terminal 
                     iinker sequence"
                     /translation="VSTGEELFTGVVPFKFQLKGTINGKSFTVEGEGEGNSHEGSHKGK
                     YVCTSGKLPMSWAALGTSFGYGMKYYTKYPSGLKNWFHEVMPEGFTYDRHIQYKGDGSI
                     HAKHQHFMKNGTYHNIVEFTGQDFKENSPVLTGDMDVSLPNEVQHIPIDDGVECTVTLQ
                     YPLLSDESKCVEAYQNTIIKPLHNQPAPDVPFHWIRKQYTQSKDDTEERDHIIQSETLE
                     AHL"
     misc_feature    2024..2612
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2077..2097)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2495..2506)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2665..2786
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2676..2695)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2730..2749
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      2901..2947
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      2977..3113
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    3158..3229
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     promoter        3274..3470
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3321..3456
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3484..4278
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3538..3557)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4148..4167
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    4289..4513
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(4709..5297)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4789..4808)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        6043..6147
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6148..6216,6217..7008)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6366..6385)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      7021..7157
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    7302..7373
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(7378..7424)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    7442..7490
                     /note="synthetic polyadenylation signal"
     enhancer        7571..7874
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7876..8078
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8029..8049
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          8212..8687
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     regulatory      join(8743..8744,1..8)
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 caccatggcg gcccccggca gcgcccggcg acccctgctg ctgctactgc tgttgctgct
       61 gctcggcctc atgcattgtg cgtcagcagc aatgtttatg gtgaaaaatg gcaacgggac
      121 cgcgtgcata atggccaact tctctgctgc cttctcagtg aactacgaca ccaagagtgg
      181 ccctaagaac atgacctttg acctgccatc agatgccaca gtggtgctca accgcagctc
      241 ctgtggaaaa gagaacactt ctgaccccag tctcgtgatt gcttttggaa gaggacatac
      301 actcactctc aatttcacga gaaatgcaac acgttacagc gtccagctca tgagttttgt
      361 ttataacttg tcagacacac accttttccc caatgcgagc tccaaagaaa tcaagactgt
      421 ggaatctata actgacatca gggcagatat agataaaaaa tacagatgtg ttagtggcac
      481 ccaggtccac atgaacaacg tgaccgtaac gctccatgat gccaccatcc aggcgtacct
      541 ttccaacagc agcttcagca ggggagagac acgctgtgaa caagacaggc cttccccaac
      601 cacagcgccc cctgcgccac ccagcccctc gccctcaccc gtgcccaaga gcccctctgt
      661 ggacaagtac aacgtgagcg gcaccaacgg gacctgcctg ctggccagca tggggctgca
      721 gctgaacctc acctatgaga ggaaggacaa cacgacggtg acaaggcttc tcaacatcaa
      781 ccccaacaag acctcggcca gcgggagctg cggcgcccac ctggtgactc tggagctgca
      841 cagcgagggc accaccgtcc tgctcttcca gttcgggatg aatgcaagtt ctagccggtt
      901 tttcctacaa ggaatccagt tgaatacaat tcttcctgac gccagagacc ctgcctttaa
      961 agctgccaac ggctccctgc gagcgctgca ggccacagtc ggcaattcct acaagtgcaa
     1021 cgcggaggag cacgtccgtg tcacgaaggc gttttcagtc aatatattca aagtgtgggt
     1081 ccaggctttc aaggtggaag gtggccagtt tggctctgtg gaggagtgtc tgctggacga
     1141 gaacagcatg ctgatcccca tcgctgtggg tggtgccctg gcggggctgg tcctcatcgt
     1201 cctcatcgcc tacctcgtcg gcaggaagag gagtcacgca ggctaccaga ctatcggagc
     1261 atcgggagcc tcaggagcat cggtgtctac aggcgaggag ctgtttaccg gcgtggtgcc
     1321 cttcaagttc cagctgaagg gcaccatcaa cggcaagagc ttcaccgtgg aaggggaagg
     1381 tgaagggaac agccacgagg gcagccacaa aggcaagtac gtgtgcacca gcggcaaact
     1441 gccaatgtct tgggccgccc tgggaactag cttcggctat ggcatgaagt actacaccaa
     1501 gtaccccagc ggcctgaaga actggttcca cgaggtgatg ccggaagggt ttacttacga
     1561 cagacacatc cagtacaagg gcgacggcag catccacgcc aagcaccagc acttcatgaa
     1621 gaacggcacc taccacaaca tcgtggagtt caccggccag gacttcaagg agaacagccc
     1681 cgtgctgacc ggcgacatgg acgtgagcct gcccaacgag gtgcagcaca tccccataga
     1741 tgacggcgtg gagtgcacag tgaccctgca gtaccctctg ctgagcgacg agagcaagtg
     1801 cgtggaggcc taccagaaca ccatcatcaa gcccctgcac aatcagccag cccccgatgt
     1861 gccattccac tggatcagaa agcagtacac ccagagcaag gacgacaccg aggagagaga
     1921 ccacatcatc cagagcgaga ccctggaggc ccacctgtaa tctagaagtt gtctcctcct
     1981 gcactgactg actgatacaa tcgatttctg gatccgcagg cctaatcaac ctctggatta
     2041 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     2101 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     2161 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     2221 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     2281 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     2341 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     2401 cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg
     2461 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     2521 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     2581 gagtcggatc tccctttggg ccgcctcccc gcctgtctag cttgactgac tgagatacag
     2641 cgtaccttca gctcacagac atgataagat acattgatga gtttggacaa accacaacta
     2701 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     2761 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
     2821 ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtattg
     2881 gcccatctct atcggtatcg tagcataacc ccttggggcc tctaaacggg tcttgagggg
     2941 ttttttgtgc ccctcgggcc ggattgctat ctaccggcat tggcgcagaa aaaaatgcct
     3001 gatgcgacgc tgcgcgtctt atactcccac atatgccaga ttcagcaacg gatacggctt
     3061 ccccaacttg cccacttcca tacgtgtcct ccttaccaga aatttatcct taaggtcgtc
     3121 agctatcctg caggcgatct ctcgatttcg atcaagacat tcctttaatg gtcttttctg
     3181 gacaccacta ggggtcagaa gtagttcatc aaactttctt ccctccctaa tctcattggt
     3241 taccttgggc tatcgaaact taattaagcg atctgcatct caattagtca gcaaccatag
     3301 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     3361 cccatcgctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     3421 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa ggaggtagcc
     3481 aacatgattg aacaagatgg attgcacgca ggttctcccg ccgcttgggt ggagaggcta
     3541 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
     3601 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
     3661 ctccaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
     3721 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
     3781 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
     3841 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
     3901 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
     3961 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcggatgccc
     4021 gacggcgagg atctcgtcgt gacccacggc gatgcctgct tgccgaatat catggtggaa
     4081 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
     4141 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
     4201 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
     4261 cttgacgagt tcttctagta tgtaagccct gtgccttcta gttgccagcc atctgttgtt
     4321 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     4381 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     4441 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     4501 gtgggctcta tggttaatta accagtcaag tcagctactt ggcgagatcg acttgtctgg
     4561 gtttcgacta cgctcagaat tgcgtcagtc aagttcgatc tggtccttgc tattgcaccc
     4621 gttctccgat tacgagtttc atttaaatca tgtgagcaaa aggccagcaa aaggccagga
     4681 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     4741 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     4801 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     4861 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     4921 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     4981 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     5041 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     5101 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     5161 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     5221 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     5281 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     5341 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     5401 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     5461 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     5521 catccatagt tgcatttaaa tttccgaact ctccaaggcc ctcgtcggaa aatcttcaaa
     5581 cctttcgtcc gatccatctt gcaggctacc tctcgaacga actatcgcaa gtctcttggc
     5641 cggccttgcg ccttggctat tgcttggcag cgcctatcgc caggtattac tccaatcccg
     5701 aatatccgag atcgggatca cccgagagaa gttcaaccta catcctcaat cccgatctat
     5761 ccgagatccg aggaatatcg aaatcggggc gcgcctggtg taccgagaac gatcctctca
     5821 gtgcgagtct cgacgatcca tatcgttgct tggcagtcag ccagtcggaa tccagcttgg
     5881 gacccaggaa gtccaatcgt cagatattgt actcaagcct ggtcacggca gcgtaccgat
     5941 ctgtttaaac ctagatattg atagtctgat cggtcaacgt ataatcgagt cctagctttt
     6001 gcaaacatct atcaagagac aggatcagca ggaggctttc gccgcggaac ccctatttgt
     6061 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     6121 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     6181 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     6241 aaagatgctg aagatcagtt gggtgcgcga gtgggttaca tcgaactgga tctcaacagc
     6301 ggtaagatcc ttgagagttt tcgccccgaa gaacgctttc caatgatgag cacttttaaa
     6361 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     6421 cgcatacact attctcagaa tgacttggtt gagtattcac cagtcacaga aaagcatctt
     6481 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     6541 gcggccaact tacttctgac aacgattgga ggaccgaagg agctaaccgc ttttttgcac
     6601 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     6661 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacctt gcgtaaacta
     6721 ttaactggcg aactacttac tctagcttcc cggcaacagt tgatagactg gatggaggcg
     6781 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     6841 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     6901 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     6961 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaacc gattctaggt
     7021 gcattggcgc agaaaaaaat gcctgatgcg acgctgcgcg tcttatactc ccacatatgc
     7081 cagattcagc aacggatacg gcttccccaa cttgcccact tccatacgtg tcctccttac
     7141 cagaaattta tccttaagat cgtttaaact cgactctggc tctatcgaat ctccgtcgtt
     7201 tcgagcttac gcgaacagcc gtggcgctca tttgctcgtc gggcatcgaa tctcgtcagc
     7261 tatcgtcagc ttaccttttt ggcagcgatc gcggctcccg acatcttgga ccattagctc
     7321 cacaggtatc ttcttccctc tagtggtcat aacagcagct tcagctacct ctcaattcaa
     7381 aaaacccctc aagacccgtt tagaggcccc aaggggttat gctatcaatc gttgcgttac
     7441 acacacaaaa aaccaacaca catccatctt cgatggatag cgattttatt atctaactgc
     7501 tgatcgagtg tagccagatc tagtaatcaa ttacggggtc attagttcat agcccatata
     7561 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     7621 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     7681 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     7741 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     7801 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     7861 tcgctattac catgctgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     7921 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     7981 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     8041 gtaggcgtgt acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcg
     8101 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc
     8161 tcccctcgaa gcttacatgt ggtaccgagc tcggatcctg agaacttcag ggtgagtcta
     8221 tgggaccctt gatgttttct ttccccttct tttctatggt taagttcatg tcataggaag
     8281 gggagaagta acagggtaca catattgacc aaatcagggt aattttgcat ttgtaatttt
     8341 aaaaaatgct ttcttctttt aatatacttt tttgtttatc ttatttctaa tactttccct
     8401 aatctctttc tttcagggca ataatgatac aatgtatcat gcctctttgc accattctaa
     8461 agaataacag tgataatttc tgggttaagg caatagcaat atttctgcat ataaatattt
     8521 ctgcatataa attgtaactg atgtaagagg tttcatattg ctaatagcag ctacaatcca
     8581 gctaccattc tgcttttatt ttatggttgg gataaggctg gattattctg agtccaagct
     8641 aggccctttt gctaatcatg ttcatacctc ttatcttcct cccacagctc ctgggcaacg
     8701 tgctggtctg tgtgctggcc catcactttg gcaaaggcgg ccgc
//
