LOCUS       sequence-438146-       11393 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive ectopic expression of human PTPN14 C1121S mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11393)
  AUTHORS   Blakely WJ, Hatterschide J, White EA
  TITLE     HPV18 E7 inhibits LATS1 kinase and activates YAP1 by degrading 
            PTPN14.
  JOURNAL   mBio. 2024 Sep 9:e0181124. doi: 10.1128/mbio.01811-24.
  PUBMED    39248565
REFERENCE   2  (bases 1 to 11393)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio. 2024 
            Sep 9:e0181124. doi: 10.1128/mbio.01811-24."
COMMENT     Sequence Label: p8189 pHAGE N-V5 PTPN14 C1121S
FEATURES             Location/Qualifiers
     source          1..11393
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    665..790
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1287..1520
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1705..1749
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2042..2159
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2211..2514
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2515..2718
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2669..2689
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2720..2738
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2741..2759
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2769..2793
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2880..2889
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2892..2933
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    2949..2973
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6538..6562)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        6581..7080
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6616..6639)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7013..7032
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7101..7700
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7101..7120)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7597..7617
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7767..8355
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7820..7840)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8238..8249)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8430..8663
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8782..8800)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8868..8972
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8973..9041,9042..9833)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9191..9210)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10004..10592
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10493..10512
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10746..10763
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11003..11024
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11039..11044,11045..11062,11063..11069)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11077..11093
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11082..11104
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11101..11117
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11101..11117
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11167..11186
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(11378..11393,1..618)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 ctcccaaaga agacaagata tccttgatct gtggatctac cacacacaag gctacttccc
       61 tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct ttggatggtg
      121 ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag gagagaacac
      181 cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag aagtgttaga
      241 gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc atccggagta
      301 cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg actttccagg
      361 gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca tataagcagc
      421 tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
      481 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
      541 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
      601 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga
      661 ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg
      721 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg
      781 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag
      841 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga
      901 acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg
      961 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt
     1021 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga
     1081 caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccggccgctg
     1141 atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat
     1201 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
     1261 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca
     1321 ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct
     1381 ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg
     1441 caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac
     1501 ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact
     1561 gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg
     1621 acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt
     1681 gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg
     1741 gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa attattcata
     1801 atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat
     1861 agagttaggc agggatattc accattatcg tttcagaccc acctcccaac cccgagggga
     1921 cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt
     1981 cgattagtga acggatctcg acggtatcgc cgaattcaca aatggcagta ttcatccaca
     2041 attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     2101 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc
     2161 gggtttatta cagggacagc agagatccag tttggactag tggagttccg cgttacataa
     2221 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
     2281 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
     2341 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
     2401 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
     2461 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg
     2521 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
     2581 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
     2641 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
     2701 gtctatataa gcagagctct ccctatcagt gatagagatc tccctatcag tgatagagat
     2761 cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
     2821 gacctccata gaagacaccg ggaccgatcc agcctccgga ctctagcgtt taaacgctag
     2881 ccaccatggc gggtaagcct atccctaacc ctctcctcgg tctcgattct acggctcgag
     2941 gttatcaaac aagtttgtac aaaaaagttg gcaccatgcc ttttggtctg aagctccgcc
     3001 ggacacggcg ctacaacgtc ctgagcaaga actgctttgt cacacggatt cgcctgctgg
     3061 acagcaatgt tatcgagtgc acgctgtcgg tggaaagcac agggcaagaa tgcctggagg
     3121 ctgtggccca gaggctggag ctgcgagaga cgcactactt tggcctttgg tttctcagca
     3181 agagccagca agcacgatgg gtggagctgg agaaacctct gaagaaacat ctggacaaat
     3241 tcgctaatga gcctttgctt ttctttggag tcatgttcta tgtgccaaat gtgtcatggc
     3301 ttcagcaaga ggccacaaga tatcagtatt acctgcaagt caaaaaagat gtgcttgaag
     3361 ggcgattacg atgtacattg gaccaggtga ttcggctagc cggcctagct gtgcaagctg
     3421 attttggaga ctataatcag tttgattctc aagatttcct cagagagtat gtgctatttc
     3481 ctatggattt ggccctggaa gaggctgttc tggaggagct gacccagaag gtagcccaag
     3541 aacacaaagc ccacagtgga atcctgccag cagaagctga actgatgtac atcaatgaag
     3601 ttgaacgttt ggatggattt ggacaggaaa tcttccctgt aaaggacaat catggaaact
     3661 gtgtacacct tggcattttc tttatgggga ttttcgtgag gaacagaatt ggaagacaag
     3721 cggtaatata caggtggaat gacatgggga atatcactca taacaagtcg accattctag
     3781 tggagctcat caacaaagaa gagactgccc tctttcacac ggatgatatc gaaaatgcca
     3841 agtatatttc tcggttgttt gccacacgac acaagtttta caaacaaaac aaaatctgca
     3901 ctgaacagtc aaattctcca ccccccatca gacgccagcc cacctggagc cggtcctctc
     3961 tgcccaggca gcagccgtac atcctgcctc ccgttcacgt ccagtgtggt gagcactact
     4021 cggaaacgca cacctcgcaa gacagcattt ttcatgggaa tgaagaagcc ttgtattgca
     4081 actctcacaa cagcctggac ttaaattatt taaatggcac tgtcaccaat ggcagcgtgt
     4141 gtagcgttca cagcgtcaac tccctcaact gctcgcaaag tttcatccag gcctcccctg
     4201 tatcctccaa cctcagtatc cctgggagtg acatcatgcg ggccgactac atcccgagcc
     4261 accggcacag cgcgatcatc gtgccctcgt acaggccaac ccccgattat gagacagtca
     4321 tgcgccagat gaagaggggg atcctgcata cagacagcca gagccagtct ctgagaaacc
     4381 tcaacattat caacacccat gcctacaacc agccagagga tctggtgtac agccaaccgg
     4441 agatgcggga gaggcacccc tacactgtcc cttatgggcc acagggggtc tacagcaaca
     4501 aacttgtcag tccatctgac cagaggaacc caaagaataa tgtggtacca agcaagccgg
     4561 gggcaagcgc catctcgcac acggtgagca ccccagagct ggccaacatg cagctgcagg
     4621 gcagccataa ctacagcacg gcccacatgc ttaagaacta tctcttcagg ccaccgcccc
     4681 cctacccacg gccacgacct gccaccagca ccccagacct ggccagccac cgccacaagt
     4741 acgtcagcgg cagcagcccg gacctggtga cccggaaggt gcagctctcg gtgaagacct
     4801 tccaagagga cagctctccg gtggttcatc agtctctcca ggaggtgagc gagcccctca
     4861 cggccaccaa gcaccacggc actgtgaaca agcgccacag cctggaggtg atgaacagca
     4921 tggtgcgggg catggaggcc atgacgctca agtcgctcca cctccccatg gctcgccgca
     4981 acacgctccg ggagcaggga ccgcccgagg aggggtcagg cagccacgag gtcccccagc
     5041 tccctcagta tcaccacaag aagaccttct ctgatgccac tatgctaatc cacagcagcg
     5101 agagtgagga ggaggaggag gaggctccag aatcggtgcc ccagatcccc atgctccggg
     5161 agaagatgga gtacagtgcc cagctgcagg cggccctggc ccgcatcccc aacaagcccc
     5221 cgcctgagta ccccggtcca aggaagagtg tgagcaatgg ggctctgagg caggaccaag
     5281 ccagccttcc tcccgccatg gccagagcca gggtgctgag gcatgggcca gccaaggcca
     5341 tcagcatgtc tcggactgac ccgccggctg tcaacggggc ctctctcggc ccatccatct
     5401 cggaacccga cctgactagt gtgaaggagc gggtcaaaaa agagcctgtg aaggagagac
     5461 ctgtgtctga aatgttttcc ctggaagaca gcattataga gagagagatg atgatcagga
     5521 atctagagaa gcagaagatg gcaggcctgg aggcacagaa gaggccgctg atgttggcag
     5581 cattgaatgg gctctcggtg gctcgagtct cagggcggga agagaatcga gttgatgcca
     5641 cccgggttcc catggacgag aggttcagaa ccctgaagaa gaaactagaa gagggaatgg
     5701 tgttcacaga atatgagcaa attccaaaga aaaaggcgaa tggcattttc agcacagcag
     5761 ctctgccaga aaacgccgag cgcagccgaa tccgtgaagt tgtcccctat gaggagaatc
     5821 gagtagagct gataccaacc aaagaaaata acacaggata cattaatgcc tcccacatca
     5881 aggtggtggt tggcggggca gaatggcact acatagccac ccaggggccc ctgccacaca
     5941 cgtgccacga cttctggcag atggtgtggg agcagggagt gaatgtgatt gccatggtca
     6001 ctgcagagga ggagggtgga cgaaccaaaa gccaccgata ctggcccaaa ctaggttcaa
     6061 agcacagctc agccacctat ggcaagttca aggtcaccac gaagtttcga acggattctg
     6121 tttgctatgc aaccacgggc ttgaaggtca agcacctttt gtctgggcaa gaaaggacgg
     6181 tgtggcattt acaatatact gactggccag atcacggctg tccagaggat gtccaaggat
     6241 ttttatccta cttggaggag atccagtcgg tccgtcgcca taccaacagc atgctggaag
     6301 gcaccaagaa ccggcacccg cccatcgtgg tccactccag tgctggggtg ggaaggaccg
     6361 gcgtgctcat tctttctgag ctgatgatct actgcttgga acataacgaa aaggtggaag
     6421 tgcccatgat gctgaggctc ctcagggagc agaggatgtt catgatccag actatcgctc
     6481 agtacaagtt tgtctaccaa gtcctcatcc agttcctcca aaactccaga ctcattttgc
     6541 caactttctt gtacaaagtg gttcgataac gaattctacc gggtagggga ggcgcttttc
     6601 ccaaggcagt ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt
     6661 ggcctctggc ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt
     6721 ggtggcccct tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg
     6781 cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag
     6841 atggacagca ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag
     6901 ctttgctcct tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc
     6961 aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc
     7021 acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg
     7081 acctgcagcc caagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg
     7141 acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc
     7201 gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc
     7261 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg
     7321 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc
     7381 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg
     7441 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc
     7501 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg
     7561 ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc
     7621 tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga
     7681 cccgcaagcc cggtgcctga cgcccgcccc acgacccgca gcgcccgacc gaaaggagcg
     7741 cacgacccca tgcatcgata gatcctaatc aacctctgga ttacaaaatt tgtgaaagat
     7801 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     7861 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     7921 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     7981 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     8041 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     8101 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     8161 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     8221 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     8281 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     8341 gggccgcctc cccgcctgag atcctttaag accaatgact tacaaggcag ctgtagatct
     8401 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     8461 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     8521 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     8581 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     8641 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
     8701 acttgcaaag aaatgaatat cagagagtga gaggcccggg ttaattaagg aaagggctag
     8761 atcattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     8821 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     8881 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     8941 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     9001 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     9061 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     9121 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     9181 ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg
     9241 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     9301 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     9361 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     9421 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     9481 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     9541 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     9601 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     9661 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     9721 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     9781 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     9841 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     9901 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     9961 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
    10021 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
    10081 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
    10141 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
    10201 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
    10261 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
    10321 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
    10381 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
    10441 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
    10501 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
    10561 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
    10621 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
    10681 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
    10741 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
    10801 ccgcgcgttg gccgattcat taatgcagca agctcatggc tgactaattt tttttattta
    10861 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
    10921 tggaggccta ggcttttgca aaaagctccc cgtggcacga caggtttccc gactggaaag
    10981 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
    11041 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
    11101 caggaaacag ctatgacatg attacgaatt tcacaaataa agcatttttt tcactgcatt
    11161 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa
    11221 ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt
    11281 acctgtggtt tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa
    11341 attgtatttg ttaaatatgt actacaaact tagtagttgg aagggctaat tca
//
