LOCUS       sequence-438148-       11273 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycycline-inducible overexpression of human PTPN14 Delta-PPXY1/2 
            mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11273)
  AUTHORS   Blakely WJ, Hatterschide J, White EA
  TITLE     HPV18 E7 inhibits LATS1 kinase and activates YAP1 by degrading 
            PTPN14.
  JOURNAL   mBio. 2024 Sep 9:e0181124. doi: 10.1128/mbio.01811-24.
  PUBMED    39248565
REFERENCE   2  (bases 1 to 11273)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio. 2024 
            Sep 9:e0181124. doi: 10.1128/mbio.01811-24."
COMMENT     Sequence Label: p8224 pLIX-PTPN14 Delta-PPXY1/2
FEATURES             Location/Qualifiers
     source          1..11273
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        47..361
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    55..73
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    91..109
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    126..144
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    162..180
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    198..216
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    233..251
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    269..287
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     334..358
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    390..414
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3949..3973)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3981..4007
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4023..4040
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        4050..4560
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4479..4497
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             4570..5166
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4570..4589)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5066..5086
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             5167..5220
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(5221..5838,5839..5844,5845..5880,5881..5883,5884..5919,
                     5920..5922,5923..5958,5959..5967)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(6007..6027)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6425..6436)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6641..6874
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6946..7067
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6983..7002)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7037..7056
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7107..7242
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        7242..7313
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7314..7382,7383..8174)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7532..7551)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8345..8933
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8834..8853
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9087..9104
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9221..9242
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9257..9262,9263..9280,9281..9287)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9295..9311
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9300..9322
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9319..9335
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9319..9335
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9354..9374
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9356..9374
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9402..9628
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9629..9809
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9856..9981
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10474..10707
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10892..10936
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11149..11266
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 attacaggga cagcagagat ccactttggc cgcgaatcga tatgtcgagt ttactcccta
       61 tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa cgatgtcgag
      121 tttactccct atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga
      181 acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgagtt tatccctatc
      241 agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg tatgtcgagg
      301 taggcgtgta cggtgggagg cctatataag cagagctcgt ttagtgaacc gtcagatcgc
      361 ctggagaatt ggctagcatc gattgatcaa caagtttgta caaaaaagtt ggcaccatgc
      421 cttttggtct gaagctccgc cggacacggc gctacaacgt cctgagcaag aactgctttg
      481 tcacacggat tcgcctgctg gacagcaatg ttatcgagtg cacgctgtcg gtggaaagca
      541 cagggcaaga atgcctggag gctgtggccc agaggctgga gctgcgagag acgcactact
      601 ttggcctttg gtttctcagc aagagccagc aagcacgatg ggtggagctg gagaaacctc
      661 tgaagaaaca tctggacaaa ttcgctaatg agcctttgct tttctttgga gtcatgttct
      721 atgtgccaaa tgtgtcatgg cttcagcaag aggccacaag atatcagtat tacctgcaag
      781 tcaaaaaaga tgtgcttgaa gggcgattac gatgtacatt ggaccaggtg attcggctag
      841 ccggcctagc tgtgcaagct gattttggag actataatca gtttgattct caagatttcc
      901 tcagagagta tgtgctattt cctatggatt tggccctgga agaggctgtt ctggaggagc
      961 tgacccagaa ggtagcccaa gaacacaaag cccacagtgg aatcctgcca gcagaagctg
     1021 aactgatgta catcaatgaa gttgaacgtt tggatggatt tggacaggaa atcttccctg
     1081 taaaggacaa tcatggaaac tgtgtacacc ttggcatttt ctttatgggg attttcgtga
     1141 ggaacagaat tggaagacaa gcggtaatat acaggtggaa tgacatgggg aatatcactc
     1201 ataacaagtc gaccattcta gtggagctca tcaacaaaga agagactgcc ctctttcaca
     1261 cggatgatat cgaaaatgcc aagtatattt ctcggttgtt tgccacacga cacaagtttt
     1321 acaaacaaaa caaaatctgc actgaacagt caaattctcc accccccatc agacgccagc
     1381 ccacctggag ccggtcctct ctgcccaggc agcagccgta catcctgcct cccgttcacg
     1441 tccagtgtgg tgagcactac tcggaaacgc acacctcgca agacagcatt tttcatggga
     1501 atgaagaagc cttgtattgc aactctcaca acagcctgga cttaaattat ttaaatggca
     1561 ctgtcaccaa tggcagcgtg tgtagcgttc acagcgtcaa ctccctcaac tgctcgcaaa
     1621 gtttcatcca ggcctcccct gtatcctcca acctcagtat ccctgggagt gacatcatgc
     1681 gggccgacta catcccgagc caccggcaca gcgcgatcat cgagacagtc atgcgccaga
     1741 tgaagagggg gatcctgcat acagacagcc agagccagtc tctgagaaac ctcaacatta
     1801 tcaacaccca tgcctacaac cagccagagg atctggtgta cagccaaccg gagatgcggg
     1861 agaggcaccc ctacactgtc ccttatgggc cacagggggt ctacagcaac aaacttgtca
     1921 gtccatctga ccagaggaac ccaaagaata atgtggtacc aagcaagccg ggggcaagcg
     1981 ccatctcgca cacggtgagc accccagagc tggccaacat gcagctgcag ggcagccata
     2041 actacagcac ggcccacatg cttaagaact atctcttcag gccaccgccc ccctacccac
     2101 ggccacgacc tgccaccagc accccagacc tggccagcca ccgccacaag tacgtcagcg
     2161 gcagcagccc ggacctggtg acccggaagg tgcagctctc ggtgaagacc ttccaagagg
     2221 acagctctcc ggtggttcat cagtctctcc aggaggtgag cgagcccctc acggccacca
     2281 agcaccacgg cactgtgaac aagcgccaca gcctggaggt gatgaacagc atggtgcggg
     2341 gcatggaggc catgacgctc aagtcgctcc acctccccat ggctcgccgc aacacgctcc
     2401 gggagcaggg accgcccgag gaggggtcag gcagccacga ggtcccccag ctccctcagt
     2461 atcaccacaa gaagaccttc tctgatgcca ctatgctaat ccacagcagc gagagtgagg
     2521 aggaggagga ggaggctcca gaatcggtgc cccagatccc catgctccgg gagaagatgg
     2581 agtacagtgc ccagctgcag gcggccctgg cccgcatccc caacaagccc ccgcctgagt
     2641 accccggtcc aaggaagagt gtgagcaatg gggctctgag gcaggaccaa gccagccttc
     2701 ctcccgccat ggccagagcc agggtgctga ggcatgggcc agccaaggcc atcagcatgt
     2761 ctcggactga cccgccggct gtcaacgggg cctctctcgg cccatccatc tcggaacccg
     2821 acctgactag tgtgaaggag cgggtcaaaa aagagcctgt gaaggagaga cctgtgtctg
     2881 aaatgttttc cctggaagac agcattatag agagagagat gatgatcagg aatctagaga
     2941 agcagaagat ggcaggcctg gaggcacaga agaggccgct gatgttggca gcattgaatg
     3001 ggctctcggt ggctcgagtc tcagggcggg aagagaatcg agttgatgcc acccgggttc
     3061 ccatggacga gaggttcaga accctgaaga agaaactaga agagggaatg gtgttcacag
     3121 aatatgagca aattccaaag aaaaaggcga atggcatttt cagcacagca gctctgccag
     3181 aaaacgccga gcgcagccga atccgtgaag ttgtccccta tgaggagaat cgagtagagc
     3241 tgataccaac caaagaaaat aacacaggat acattaatgc ctcccacatc aaggtggtgg
     3301 ttggcggggc agaatggcac tacatagcca cccaggggcc cctgccacac acgtgccacg
     3361 acttctggca gatggtgtgg gagcagggag tgaatgtgat tgccatggtc actgcagagg
     3421 aggagggtgg acgaaccaaa agccaccgat actggcccaa actaggttca aagcacagct
     3481 cagccaccta tggcaagttc aaggtcacca cgaagtttcg aacggattct gtttgctatg
     3541 caaccacggg cttgaaggtc aagcaccttt tgtctgggca agaaaggacg gtgtggcatt
     3601 tacaatatac tgactggcca gatcacggct gtccagagga tgtccaagga tttttatcct
     3661 acttggagga gatccagtcg gtccgtcgcc ataccaacag catgctggaa ggcaccaaga
     3721 accggcaccc gcccatcgtg gtccactgta gtgctggggt gggaaggacc ggcgtgctca
     3781 ttctttctga gctgatgatc tactgcttgg aacataacga aaaggtggaa gtgcccatga
     3841 tgctgaggct cctcagggag cagaggatgt tcatgatcca gactatcgct cagtacaagt
     3901 ttgtctacca agtcctcatc cagttcctcc aaaactccag actcattttg ccaactttct
     3961 tgtacaaagt ggttaccggt tatccgtatg atgtgccgga ttatgcgtaa acgcgtccgg
     4021 tccaccacca ccaccaccac taaggatccg gggttggggt tgcgcctttt ccaaggcagc
     4081 cctgggtttg cgcagggacg cggctgctct gggcgtggtt ccgggaaacg cagcggcgcc
     4141 gaccctgggt ctcgcacatt cttcacgtcc gttcgcagcg tcacccggat cttcgccgct
     4201 acccttgtgg gccccccggc gacgcttcct gctccgcccc taagtcggga aggttccttg
     4261 cggttcgcgg cgtgccggac gtgacaaacg gaagccgcac gtctcactag taccctcgca
     4321 gacggacagc gccagggagc aatggcagcg cgccgaccgc gatgggctgt ggccaatagc
     4381 ggctgctcag cagggcgcgc cgagagcagc ggccgggaag gggcggtgcg ggaggcgggg
     4441 tgtggggcgg tagtgtgggc cctgttcctg cccgcgcggt gttccgcatt ctgcaagcct
     4501 ccggagcgca cgtcggcagt cggctccctc gttgaccgaa tcaccgacct ctctccccag
     4561 caattcacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc
     4621 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc
     4681 gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc
     4741 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc
     4801 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag
     4861 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg
     4921 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag
     4981 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc
     5041 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc
     5101 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc
     5161 ggtgccgaag gtagaggttc tctcctcact tgtggtgatg ttgaagaaaa ccctggtcca
     5221 atgtctagac tggacaagag caaagtcata aacggagctc tggaattact caatggtgtc
     5281 ggtatcgaag gcctgacgac aaggaaactc gctcaaaagc tgggagttga gcagcctacc
     5341 ctgtactggc acgtgaagaa caagcgggcc ctgctcgatg ccctgccaat cgagatgctg
     5401 gacaggcatc atacccactt ctgccccctg gaaggcgagt catggcaaga ctttctgcgg
     5461 aacaacgcca agtcataccg ctgtgctctc ctctcacatc gcgacggggc taaagtgcat
     5521 ctcggcaccc gcccaacaga gaaacagtac gaaaccctgg aaaatcagct cgcgttcctg
     5581 tgtcagcaag gcttctccct ggagaacgca ctgtacgctc tgtccgccgt gggccacttt
     5641 acactgggct gcgtattgga ggaacaggag catcaagtag caaaagagga aagagagaca
     5701 cctaccaccg attctatgcc cccacttctg agacaagcaa ttgagctgtt cgaccggcag
     5761 ggagccgaac ctgccttcct tttcggcctg gaactaatca tatgtggcct ggagaaacag
     5821 ctaaagtgcg aaagcggcgg gccgaccgac gcccttgacg attttgactt agacatgctc
     5881 ccagccgatg cccttgacga ctttgacctt gatatgctgc ctgctgacgc tcttgacgat
     5941 tttgaccttg acatgctccc cgggtaacta agtaaggatc gatccaagat atcgtattct
     6001 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     6061 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     6121 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     6181 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     6241 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     6301 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt
     6361 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     6421 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     6481 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     6541 gcctgtttcg cctcggcgtc cggactagag gtacctttaa gaccaatgac ttacaaggca
     6601 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     6661 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     6721 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     6781 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     6841 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
     6901 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca
     6961 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     7021 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc
     7081 tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     7141 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     7201 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctttcaaat atgtatccgc
     7261 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     7321 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     7381 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     7441 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     7501 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     7561 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     7621 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     7681 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     7741 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     7801 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     7861 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     7921 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     7981 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     8041 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     8101 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     8161 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     8221 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     8281 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     8341 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     8401 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     8461 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     8521 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     8581 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     8641 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     8701 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     8761 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     8821 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     8881 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     8941 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     9001 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     9061 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     9121 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     9181 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     9241 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     9301 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
     9361 accctcacta aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact
     9421 cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa
     9481 gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca
     9541 acagacgggt ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt
     9601 atttaagtgc ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg
     9661 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     9721 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     9781 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
     9841 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
     9901 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
     9961 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
    10021 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
    10081 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
    10141 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
    10201 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
    10261 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
    10321 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
    10381 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
    10441 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
    10501 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
    10561 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
    10621 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
    10681 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
    10741 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
    10801 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
    10861 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
    10921 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
    10981 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
    11041 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
    11101 gaggggacaa ttctcgacct cgagacaaat ggcagtattc atccacaatt ttaaaagaaa
    11161 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
    11221 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg ttt
//
