LOCUS       sequence-438149-       11363 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive ectopic expression of human PTPN14 Delta-PPXY1/2 
            mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11363)
  AUTHORS   Blakely WJ, Hatterschide J, White EA
  TITLE     HPV18 E7 inhibits LATS1 kinase and activates YAP1 by degrading 
            PTPN14.
  JOURNAL   mBio. 2024 Sep 9:e0181124. doi: 10.1128/mbio.01811-24.
  PUBMED    39248565
REFERENCE   2  (bases 1 to 11363)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio. 2024 
            Sep 9:e0181124. doi: 10.1128/mbio.01811-24."
COMMENT     Sequence Label: p8215 pHAGE N-V5 PTPN14 Delta-PPXY1/2
FEATURES             Location/Qualifiers
     source          1..11363
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             3..636
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    683..808
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1305..1538
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1723..1767
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2060..2177
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2229..2532
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2533..2736
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2687..2707
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2738..2756
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2759..2777
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2787..2811
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2898..2907
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2910..2951
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    2967..2991
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6526..6550)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        6569..7068
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6604..6627)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7001..7020
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7089..7688
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7089..7108)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7585..7605
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7755..8343
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7808..7828)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8226..8237)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8418..8651
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8770..8788)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8856..8960
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8961..9029,9030..9821)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9179..9198)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9992..10580
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10481..10500
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10734..10751
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10991..11012
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11027..11032,11033..11050,11051..11057)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11065..11081
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11070..11092
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11089..11105
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11089..11105
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11155..11174
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 gttggaaggg ctaattcact cccaaagaag acaagatatc cttgatctgt ggatctacca
       61 cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc
      121 actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc
      181 caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc
      241 ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg
      301 agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca agggactttc
      361 cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag
      421 atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct
      481 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
      541 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
      601 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
      661 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
      721 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
      781 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
      841 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
      901 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
      961 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     1021 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     1081 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     1141 gcaagcggcc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga
     1201 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
     1261 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
     1321 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
     1381 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
     1441 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
     1501 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
     1561 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
     1621 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
     1681 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
     1741 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
     1801 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
     1861 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
     1921 ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga
     1981 gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgccg aattcacaaa
     2041 tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg
     2101 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
     2161 caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt tggactagtg
     2221 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     2281 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     2341 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     2401 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     2461 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     2521 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     2581 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     2641 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     2701 aggcgtgtac ggtgggaggt ctatataagc agagctctcc ctatcagtga tagagatctc
     2761 cctatcagtg atagagatcg tcgacgagct cgtttagtga accgtcagat cgcctggaga
     2821 cgccatccac gctgttttga cctccataga agacaccggg accgatccag cctccggact
     2881 ctagcgttta aacgctagcc accatggcgg gtaagcctat ccctaaccct ctcctcggtc
     2941 tcgattctac ggctcgaggt tatcaaacaa gtttgtacaa aaaagttggc accatgcctt
     3001 ttggtctgaa gctccgccgg acacggcgct acaacgtcct gagcaagaac tgctttgtca
     3061 cacggattcg cctgctggac agcaatgtta tcgagtgcac gctgtcggtg gaaagcacag
     3121 ggcaagaatg cctggaggct gtggcccaga ggctggagct gcgagagacg cactactttg
     3181 gcctttggtt tctcagcaag agccagcaag cacgatgggt ggagctggag aaacctctga
     3241 agaaacatct ggacaaattc gctaatgagc ctttgctttt ctttggagtc atgttctatg
     3301 tgccaaatgt gtcatggctt cagcaagagg ccacaagata tcagtattac ctgcaagtca
     3361 aaaaagatgt gcttgaaggg cgattacgat gtacattgga ccaggtgatt cggctagccg
     3421 gcctagctgt gcaagctgat tttggagact ataatcagtt tgattctcaa gatttcctca
     3481 gagagtatgt gctatttcct atggatttgg ccctggaaga ggctgttctg gaggagctga
     3541 cccagaaggt agcccaagaa cacaaagccc acagtggaat cctgccagca gaagctgaac
     3601 tgatgtacat caatgaagtt gaacgtttgg atggatttgg acaggaaatc ttccctgtaa
     3661 aggacaatca tggaaactgt gtacaccttg gcattttctt tatggggatt ttcgtgagga
     3721 acagaattgg aagacaagcg gtaatataca ggtggaatga catggggaat atcactcata
     3781 acaagtcgac cattctagtg gagctcatca acaaagaaga gactgccctc tttcacacgg
     3841 atgatatcga aaatgccaag tatatttctc ggttgtttgc cacacgacac aagttttaca
     3901 aacaaaacaa aatctgcact gaacagtcaa attctccacc ccccatcaga cgccagccca
     3961 cctggagccg gtcctctctg cccaggcagc agccgtacat cctgcctccc gttcacgtcc
     4021 agtgtggtga gcactactcg gaaacgcaca cctcgcaaga cagcattttt catgggaatg
     4081 aagaagcctt gtattgcaac tctcacaaca gcctggactt aaattattta aatggcactg
     4141 tcaccaatgg cagcgtgtgt agcgttcaca gcgtcaactc cctcaactgc tcgcaaagtt
     4201 tcatccaggc ctcccctgta tcctccaacc tcagtatccc tgggagtgac atcatgcggg
     4261 ccgactacat cccgagccac cggcacagcg cgatcatcga gacagtcatg cgccagatga
     4321 agagggggat cctgcataca gacagccaga gccagtctct gagaaacctc aacattatca
     4381 acacccatgc ctacaaccag ccagaggatc tggtgtacag ccaaccggag atgcgggaga
     4441 ggcaccccta cactgtccct tatgggccac agggggtcta cagcaacaaa cttgtcagtc
     4501 catctgacca gaggaaccca aagaataatg tggtaccaag caagccgggg gcaagcgcca
     4561 tctcgcacac ggtgagcacc ccagagctgg ccaacatgca gctgcagggc agccataact
     4621 acagcacggc ccacatgctt aagaactatc tcttcaggcc accgcccccc tacccacggc
     4681 cacgacctgc caccagcacc ccagacctgg ccagccaccg ccacaagtac gtcagcggca
     4741 gcagcccgga cctggtgacc cggaaggtgc agctctcggt gaagaccttc caagaggaca
     4801 gctctccggt ggttcatcag tctctccagg aggtgagcga gcccctcacg gccaccaagc
     4861 accacggcac tgtgaacaag cgccacagcc tggaggtgat gaacagcatg gtgcggggca
     4921 tggaggccat gacgctcaag tcgctccacc tccccatggc tcgccgcaac acgctccggg
     4981 agcagggacc gcccgaggag gggtcaggca gccacgaggt cccccagctc cctcagtatc
     5041 accacaagaa gaccttctct gatgccacta tgctaatcca cagcagcgag agtgaggagg
     5101 aggaggagga ggctccagaa tcggtgcccc agatccccat gctccgggag aagatggagt
     5161 acagtgccca gctgcaggcg gccctggccc gcatccccaa caagcccccg cctgagtacc
     5221 ccggtccaag gaagagtgtg agcaatgggg ctctgaggca ggaccaagcc agccttcctc
     5281 ccgccatggc cagagccagg gtgctgaggc atgggccagc caaggccatc agcatgtctc
     5341 ggactgaccc gccggctgtc aacggggcct ctctcggccc atccatctcg gaacccgacc
     5401 tgactagtgt gaaggagcgg gtcaaaaaag agcctgtgaa ggagagacct gtgtctgaaa
     5461 tgttttccct ggaagacagc attatagaga gagagatgat gatcaggaat ctagagaagc
     5521 agaagatggc aggcctggag gcacagaaga ggccgctgat gttggcagca ttgaatgggc
     5581 tctcggtggc tcgagtctca gggcgggaag agaatcgagt tgatgccacc cgggttccca
     5641 tggacgagag gttcagaacc ctgaagaaga aactagaaga gggaatggtg ttcacagaat
     5701 atgagcaaat tccaaagaaa aaggcgaatg gcattttcag cacagcagct ctgccagaaa
     5761 acgccgagcg cagccgaatc cgtgaagttg tcccctatga ggagaatcga gtagagctga
     5821 taccaaccaa agaaaataac acaggataca ttaatgcctc ccacatcaag gtggtggttg
     5881 gcggggcaga atggcactac atagccaccc aggggcccct gccacacacg tgccacgact
     5941 tctggcagat ggtgtgggag cagggagtga atgtgattgc catggtcact gcagaggagg
     6001 agggtggacg aaccaaaagc caccgatact ggcccaaact aggttcaaag cacagctcag
     6061 ccacctatgg caagttcaag gtcaccacga agtttcgaac ggattctgtt tgctatgcaa
     6121 ccacgggctt gaaggtcaag caccttttgt ctgggcaaga aaggacggtg tggcatttac
     6181 aatatactga ctggccagat cacggctgtc cagaggatgt ccaaggattt ttatcctact
     6241 tggaggagat ccagtcggtc cgtcgccata ccaacagcat gctggaaggc accaagaacc
     6301 ggcacccgcc catcgtggtc cactgtagtg ctggggtggg aaggaccggc gtgctcattc
     6361 tttctgagct gatgatctac tgcttggaac ataacgaaaa ggtggaagtg cccatgatgc
     6421 tgaggctcct cagggagcag aggatgttca tgatccagac tatcgctcag tacaagtttg
     6481 tctaccaagt cctcatccag ttcctccaaa actccagact cattttgcca actttcttgt
     6541 acaaagtggt tcgataacga attctaccgg gtaggggagg cgcttttccc aaggcagtct
     6601 ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct
     6661 cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc
     6721 gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg
     6781 tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc
     6841 gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc
     6901 gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc
     6961 aggggcgggg cgggcgcccg aagggcctcc ggaggcccgg cattctgcac gcttcaaaag
     7021 cgcacggctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcagcccc
     7081 agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca
     7141 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
     7201 atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
     7261 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
     7321 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
     7381 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
     7441 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
     7501 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
     7561 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
     7621 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
     7681 gtgcctgacg cccgccccac gacccgcagc gcccgaccga aaggagcgca cgaccccatg
     7741 catcgataga tcctaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     7801 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     7861 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     7921 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     7981 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     8041 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     8101 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     8161 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     8221 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     8281 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     8341 cgcctgagat cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     8401 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt
     8461 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     8521 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     8581 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     8641 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     8701 atgaatatca gagagtgaga ggcccgggtt aattaaggaa agggctagat cattcttgaa
     8761 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     8821 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     8881 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     8941 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     9001 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     9061 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     9121 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     9181 tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac
     9241 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     9301 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     9361 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     9421 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     9481 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     9541 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     9601 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     9661 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     9721 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     9781 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     9841 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     9901 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     9961 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
    10021 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
    10081 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
    10141 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
    10201 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
    10261 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
    10321 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
    10381 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
    10441 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
    10501 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
    10561 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
    10621 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
    10681 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
    10741 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
    10801 cgattcatta atgcagcaag ctcatggctg actaattttt tttatttatg cagaggccga
    10861 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
    10921 cttttgcaaa aagctccccg tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
    10981 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
    11041 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
    11101 atgacatgat tacgaatttc acaaataaag catttttttc actgcattct agttgtggtt
    11161 tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc
    11221 aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac ctgtggtttc
    11281 atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt
    11341 aaatatgtac tacaaactta gta
//
