LOCUS       sequence-438150-       11369 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive ectopic expression of human PTPN14 Delta-PPXY3/4 
            mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11369)
  AUTHORS   Blakely WJ, Hatterschide J, White EA
  TITLE     HPV18 E7 inhibits LATS1 kinase and activates YAP1 by degrading 
            PTPN14.
  JOURNAL   mBio. 2024 Sep 9:e0181124. doi: 10.1128/mbio.01811-24.
  PUBMED    39248565
REFERENCE   2  (bases 1 to 11369)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio. 2024 
            Sep 9:e0181124. doi: 10.1128/mbio.01811-24."
COMMENT     Sequence Label: p8180 pHAGE N-V5 PTPN14 Delta-PPXY3/4
FEATURES             Location/Qualifiers
     source          1..11369
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    664..789
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1286..1519
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1704..1748
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2041..2158
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2210..2513
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2514..2717
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2668..2688
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2719..2737
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2740..2758
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2768..2792
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2879..2888
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2891..2932
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    2948..2972
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6513..6537)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        6556..7055
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6591..6614)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6988..7007
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7076..7675
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7076..7095)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7572..7592
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7742..8330
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7795..7815)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8213..8224)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8405..8638
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8757..8775)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8843..8947
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8948..9016,9017..9808)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9166..9185)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9979..10567
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10468..10487
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10721..10738
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10978..10999
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11014..11019,11020..11037,11038..11044)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11052..11068
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11057..11079
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11076..11092
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11076..11092
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11142..11161
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(11353..11369,1..617)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 tcccaaagaa gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct
       61 gattagcaga actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc
      121 tacaagctag taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc
      181 agcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga agtgttagag
      241 tggaggtttg acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac
      301 ttcaagaact gctgatatcg agcttgctac aagggacttt ccgctgggga ctttccaggg
      361 aggcgtggcc tgggcgggac tggggagtgg cgagccctca gatcctgcat ataagcagct
      421 gctttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
      481 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
      541 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
      601 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag
      661 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
      721 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
      781 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg
      841 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa
      901 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga
      961 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta
     1021 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac
     1081 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cggccgctga
     1141 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata
     1201 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
     1261 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
     1321 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
     1381 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
     1441 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
     1501 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
     1561 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga
     1621 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
     1681 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
     1741 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
     1801 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
     1861 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
     1921 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc
     1981 gattagtgaa cggatctcga cggtatcgcc gaattcacaa atggcagtat tcatccacaa
     2041 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
     2101 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
     2161 ggtttattac agggacagca gagatccagt ttggactagt ggagttccgc gttacataac
     2221 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
     2281 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
     2341 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
     2401 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
     2461 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
     2521 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
     2581 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
     2641 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
     2701 tctatataag cagagctctc cctatcagtg atagagatct ccctatcagt gatagagatc
     2761 gtcgacgagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
     2821 acctccatag aagacaccgg gaccgatcca gcctccggac tctagcgttt aaacgctagc
     2881 caccatggcg ggtaagccta tccctaaccc tctcctcggt ctcgattcta cggctcgagg
     2941 ttatcaaaca agtttgtaca aaaaagttgg caccatgcct tttggtctga agctccgccg
     3001 gacacggcgc tacaacgtcc tgagcaagaa ctgctttgtc acacggattc gcctgctgga
     3061 cagcaatgtt atcgagtgca cgctgtcggt ggaaagcaca gggcaagaat gcctggaggc
     3121 tgtggcccag aggctggagc tgcgagagac gcactacttt ggcctttggt ttctcagcaa
     3181 gagccagcaa gcacgatggg tggagctgga gaaacctctg aagaaacatc tggacaaatt
     3241 cgctaatgag cctttgcttt tctttggagt catgttctat gtgccaaatg tgtcatggct
     3301 tcagcaagag gccacaagat atcagtatta cctgcaagtc aaaaaagatg tgcttgaagg
     3361 gcgattacga tgtacattgg accaggtgat tcggctagcc ggcctagctg tgcaagctga
     3421 ttttggagac tataatcagt ttgattctca agatttcctc agagagtatg tgctatttcc
     3481 tatggatttg gccctggaag aggctgttct ggaggagctg acccagaagg tagcccaaga
     3541 acacaaagcc cacagtggaa tcctgccagc agaagctgaa ctgatgtaca tcaatgaagt
     3601 tgaacgtttg gatggatttg gacaggaaat cttccctgta aaggacaatc atggaaactg
     3661 tgtacacctt ggcattttct ttatggggat tttcgtgagg aacagaattg gaagacaagc
     3721 ggtaatatac aggtggaatg acatggggaa tatcactcat aacaagtcga ccattctagt
     3781 ggagctcatc aacaaagaag agactgccct ctttcacacg gatgatatcg aaaatgccaa
     3841 gtatatttct cggttgtttg ccacacgaca caagttttac aaacaaaaca aaatctgcac
     3901 tgaacagtca aattctccac cccccatcag acgccagccc acctggagcc ggtcctctct
     3961 gcccaggcag cagccgtaca tcctgcctcc cgttcacgtc cagtgtggtg agcactactc
     4021 ggaaacgcac acctcgcaag acagcatttt tcatgggaat gaagaagcct tgtattgcaa
     4081 ctctcacaac agcctggact taaattattt aaatggcact gtcaccaatg gcagcgtgtg
     4141 tagcgttcac agcgtcaact ccctcaactg ctcgcaaagt ttcatccagg cctcccctgt
     4201 atcctccaac ctcagtatcc ctgggagtga catcatgcgg gccgactaca tcccgagcca
     4261 ccggcacagc gcgatcatcg tgccctcgta caggccaacc cccgattatg agacagtcat
     4321 gcgccagatg aagaggggga tcctgcatac agacagccag agccagtctc tgagaaacct
     4381 caacattatc aacacccatg cctacaacca gccagaggat ctggtgtaca gccaaccgga
     4441 gatgcgggag aggcacccct acactgtccc ttatgggcca cagggggtct acagcaacaa
     4501 acttgtcagt ccatctgacc agaggaaccc aaagaataat gtggtaccaa gcaagccggg
     4561 ggcaagcgcc atctcgcaca cggtgagcac cccagagctg gccaacatgc agctgcaggg
     4621 cagccataac tacagcacgg cccacatgct taagaactat ctcttcaggc caccacggcc
     4681 acgacctgcc accagcaccc cagacctggc cagccaccgc cacaagtacg tcagcggcag
     4741 cagcccggac ctggtgaccc ggaaggtgca gctctcggtg aagaccttcc aagaggacag
     4801 ctctccggtg gttcatcagt ctctccagga ggtgagcgag cccctcacgg ccaccaagca
     4861 ccacggcact gtgaacaagc gccacagcct ggaggtgatg aacagcatgg tgcggggcat
     4921 ggaggccatg acgctcaagt cgctccacct ccccatggct cgccgcaaca cgctccggga
     4981 gcagggaccg cccgaggagg ggtcaggcag ccacgaggtc ccccagctcc ctcagtatca
     5041 ccacaagaag accttctctg atgccactat gctaatccac agcagcgaga gtgaggagga
     5101 ggaggaggag gctccagaat cggtgcccca gatccccatg ctccgggaga agatggagta
     5161 cagtgcccag ctgcaggcgg ccctggcccg catccccaac aagccccccg gtccaaggaa
     5221 gagtgtgagc aatggggctc tgaggcagga ccaagccagc cttcctcccg ccatggccag
     5281 agccagggtg ctgaggcatg ggccagccaa ggccatcagc atgtctcgga ctgacccgcc
     5341 ggctgtcaac ggggcctctc tcggcccatc catctcggaa cccgacctga ctagtgtgaa
     5401 ggagcgggtc aaaaaagagc ctgtgaagga gagacctgtg tctgaaatgt tttccctgga
     5461 agacagcatt atagagagag agatgatgat caggaatcta gagaagcaga agatggcagg
     5521 cctggaggca cagaagaggc cgctgatgtt ggcagcattg aatgggctct cggtggctcg
     5581 agtctcaggg cgggaagaga atcgagttga tgccacccgg gttcccatgg acgagaggtt
     5641 cagaaccctg aagaagaaac tagaagaggg aatggtgttc acagaatatg agcaaattcc
     5701 aaagaaaaag gcgaatggca ttttcagcac agcagctctg ccagaaaacg ccgagcgcag
     5761 ccgaatccgt gaagttgtcc cctatgagga gaatcgagta gagctgatac caaccaaaga
     5821 aaataacaca ggatacatta atgcctccca catcaaggtg gtggttggcg gggcagaatg
     5881 gcactacata gccacccagg ggcccctgcc acacacgtgc cacgacttct ggcagatggt
     5941 gtgggagcag ggagtgaatg tgattgccat ggtcactgca gaggaggagg gtggacgaac
     6001 caaaagccac cgatactggc ccaaactagg ttcaaagcac agctcagcca cctatggcaa
     6061 gttcaaggtc accacgaagt ttcgaacgga ttctgtttgc tatgcaacca cgggcttgaa
     6121 ggtcaagcac cttttgtctg ggcaagaaag gacggtgtgg catttacaat atactgactg
     6181 gccagatcac ggctgtccag aggatgtcca aggattttta tcctacttgg aggagatcca
     6241 gtcggtccgt cgccatacca acagcatgct ggaaggcacc aagaaccggc acccgcccat
     6301 cgtggtccac tgtagtgctg gggtgggaag gaccggcgtg ctcattcttt ctgagctgat
     6361 gatctactgc ttggaacata acgaaaaggt ggaagtgccc atgatgctga ggctcctcag
     6421 ggagcagagg atgttcatga tccagactat cgctcagtac aagtttgtct accaagtcct
     6481 catccagttc ctccaaaact ccagactcat tttgccaact ttcttgtaca aagtggttcg
     6541 ataacgaatt ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt
     6601 tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca
     6661 catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta
     6721 ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac
     6781 aaatggaagt agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga
     6841 agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc
     6901 agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg
     6961 gcgcccgaag gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg
     7021 cgctgttctc ctcttcctca tctccgggcc tttcgacctg cagcccaagc ttaccatgac
     7081 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
     7141 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
     7201 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     7261 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt
     7321 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
     7381 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     7441 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     7501 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
     7561 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
     7621 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgacgccc
     7681 gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga ccccatgcat cgatagatcc
     7741 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     7801 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     7861 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     7921 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     7981 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     8041 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     8101 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     8161 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     8221 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     8281 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctgagatcct
     8341 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     8401 ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg
     8461 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     8521 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     8581 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     8641 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     8701 agtgagaggc ccgggttaat taaggaaagg gctagatcat tcttgaagac gaaagggcct
     8761 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     8821 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     8881 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     8941 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     9001 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     9061 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     9121 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     9181 attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     9241 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     9301 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     9361 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     9421 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     9481 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     9541 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     9601 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     9661 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     9721 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     9781 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     9841 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     9901 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     9961 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
    10021 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
    10081 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
    10141 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
    10201 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
    10261 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
    10321 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
    10381 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
    10441 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
    10501 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
    10561 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
    10621 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
    10681 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
    10741 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
    10801 cagcaagctc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
    10861 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
    10921 ctccccgtgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
    10981 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
    11041 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg acatgattac
    11101 gaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
    11161 caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa
    11221 acttcccacc ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac
    11281 ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac
    11341 aaacttagta gttggaaggg ctaattcac
//
