LOCUS       sequence-438151-       11303 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycycline-inducible overexpression of human WT PTPN14.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11303)
  AUTHORS   Blakely WJ, Hatterschide J, White EA
  TITLE     HPV18 E7 inhibits LATS1 kinase and activates YAP1 by degrading 
            PTPN14.
  JOURNAL   mBio. 2024 Sep 9:e0181124. doi: 10.1128/mbio.01811-24.
  PUBMED    39248565
REFERENCE   2  (bases 1 to 11303)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio. 2024 
            Sep 9:e0181124. doi: 10.1128/mbio.01811-24."
COMMENT     Sequence Label: p8221 pLIX-PTPN14
FEATURES             Location/Qualifiers
     source          1..11303
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        53..367
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    61..79
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    97..115
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    132..150
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    168..186
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    204..222
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    239..257
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    275..293
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     340..364
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    396..420
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3985..4009)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4017..4043
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4059..4076
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        4086..4596
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4515..4533
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             4606..5202
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4606..4625)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5102..5122
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             5203..5256
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(5257..5874,5875..5880,5881..5916,5917..5919,5920..5955,
                     5956..5958,5959..5994,5995..6003)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(6043..6063)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6461..6472)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6677..6910
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6982..7103
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7019..7038)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7073..7092
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7143..7278
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        7278..7349
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7350..7418,7419..8210)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7568..7587)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8381..8969
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8870..8889
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9123..9140
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9257..9278
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9293..9298,9299..9316,9317..9323)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9331..9347
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9336..9358
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9355..9371
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9355..9371
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9390..9410
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9392..9410
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9438..9664
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9665..9845
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9892..10017
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10510..10743
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10928..10972
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11185..11302
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gggtttatta cagggacagc agagatccac tttggccgcg aatcgatatg tcgagtttac
       61 tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgat
      121 gtcgagttta ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga
      181 tagagaacgt atgtcgagtt tactccctat cagtgataga gaacgtatgt cgagtttatc
      241 cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg
      301 tcgaggtagg cgtgtacggt gggaggccta tataagcaga gctcgtttag tgaaccgtca
      361 gatcgcctgg agaattggct agcatcgatt gatcaacaag tttgtacaaa aaagttggca
      421 ccatgccttt tggtctgaag ctccgccgga cacggcgcta caacgtcctg agcaagaact
      481 gctttgtcac acggattcgc ctgctggaca gcaatgttat cgagtgcacg ctgtcggtgg
      541 aaagcacagg gcaagaatgc ctggaggctg tggcccagag gctggagctg cgagagacgc
      601 actactttgg cctttggttt ctcagcaaga gccagcaagc acgatgggtg gagctggaga
      661 aacctctgaa gaaacatctg gacaaattcg ctaatgagcc tttgcttttc tttggagtca
      721 tgttctatgt gccaaatgtg tcatggcttc agcaagaggc cacaagatat cagtattacc
      781 tgcaagtcaa aaaagatgtg cttgaagggc gattacgatg tacattggac caggtgattc
      841 ggctagccgg cctagctgtg caagctgatt ttggagacta taatcagttt gattctcaag
      901 atttcctcag agagtatgtg ctatttccta tggatttggc cctggaagag gctgttctgg
      961 aggagctgac ccagaaggta gcccaagaac acaaagccca cagtggaatc ctgccagcag
     1021 aagctgaact gatgtacatc aatgaagttg aacgtttgga tggatttgga caggaaatct
     1081 tccctgtaaa ggacaatcat ggaaactgtg tacaccttgg cattttcttt atggggattt
     1141 tcgtgaggaa cagaattgga agacaagcgg taatatacag gtggaatgac atggggaata
     1201 tcactcataa caagtcgacc attctagtgg agctcatcaa caaagaagag actgccctct
     1261 ttcacacgga tgatatcgaa aatgccaagt atatttctcg gttgtttgcc acacgacaca
     1321 agttttacaa acaaaacaaa atctgcactg aacagtcaaa ttctccaccc cccatcagac
     1381 gccagcccac ctggagccgg tcctctctgc ccaggcagca gccgtacatc ctgcctcccg
     1441 ttcacgtcca gtgtggtgag cactactcgg aaacgcacac ctcgcaagac agcatttttc
     1501 atgggaatga agaagccttg tattgcaact ctcacaacag cctggactta aattatttaa
     1561 atggcactgt caccaatggc agcgtgtgta gcgttcacag cgtcaactcc ctcaactgct
     1621 cgcaaagttt catccaggcc tcccctgtat cctccaacct cagtatccct gggagtgaca
     1681 tcatgcgggc cgactacatc ccgagccacc ggcacagcgc gatcatcgtg ccctcgtaca
     1741 ggccaacccc cgattatgag acagtcatgc gccagatgaa gagggggatc ctgcatacag
     1801 acagccagag ccagtctctg agaaacctca acattatcaa cacccatgcc tacaaccagc
     1861 cagaggatct ggtgtacagc caaccggaga tgcgggagag gcacccctac actgtccctt
     1921 atgggccaca gggggtctac agcaacaaac ttgtcagtcc atctgaccag aggaacccaa
     1981 agaataatgt ggtaccaagc aagccggggg caagcgccat ctcgcacacg gtgagcaccc
     2041 cagagctggc caacatgcag ctgcagggca gccataacta cagcacggcc cacatgctta
     2101 agaactatct cttcaggcca ccgcccccct acccacggcc acgacctgcc accagcaccc
     2161 cagacctggc cagccaccgc cacaagtacg tcagcggcag cagcccggac ctggtgaccc
     2221 ggaaggtgca gctctcggtg aagaccttcc aagaggacag ctctccggtg gttcatcagt
     2281 ctctccagga ggtgagcgag cccctcacgg ccaccaagca ccacggcact gtgaacaagc
     2341 gccacagcct ggaggtgatg aacagcatgg tgcggggcat ggaggccatg acgctcaagt
     2401 cgctccacct ccccatggct cgccgcaaca cgctccggga gcagggaccg cccgaggagg
     2461 ggtcaggcag ccacgaggtc ccccagctcc ctcagtatca ccacaagaag accttctctg
     2521 atgccactat gctaatccac agcagcgaga gtgaggagga ggaggaggag gctccagaat
     2581 cggtgcccca gatccccatg ctccgggaga agatggagta cagtgcccag ctgcaggcgg
     2641 ccctggcccg catccccaac aagcccccgc ctgagtaccc cggtccaagg aagagtgtga
     2701 gcaatggggc tctgaggcag gaccaagcca gccttcctcc cgccatggcc agagccaggg
     2761 tgctgaggca tgggccagcc aaggccatca gcatgtctcg gactgacccg ccggctgtca
     2821 acggggcctc tctcggccca tccatctcgg aacccgacct gactagtgtg aaggagcggg
     2881 tcaaaaaaga gcctgtgaag gagagacctg tgtctgaaat gttttccctg gaagacagca
     2941 ttatagagag agagatgatg atcaggaatc tagagaagca gaagatggca ggcctggagg
     3001 cacagaagag gccgctgatg ttggcagcat tgaatgggct ctcggtggct cgagtctcag
     3061 ggcgggaaga gaatcgagtt gatgccaccc gggttcccat ggacgagagg ttcagaaccc
     3121 tgaagaagaa actagaagag ggaatggtgt tcacagaata tgagcaaatt ccaaagaaaa
     3181 aggcgaatgg cattttcagc acagcagctc tgccagaaaa cgccgagcgc agccgaatcc
     3241 gtgaagttgt cccctatgag gagaatcgag tagagctgat accaaccaaa gaaaataaca
     3301 caggatacat taatgcctcc cacatcaagg tggtggttgg cggggcagaa tggcactaca
     3361 tagccaccca ggggcccctg ccacacacgt gccacgactt ctggcagatg gtgtgggagc
     3421 agggagtgaa tgtgattgcc atggtcactg cagaggagga gggtggacga accaaaagcc
     3481 accgatactg gcccaaacta ggttcaaagc acagctcagc cacctatggc aagttcaagg
     3541 tcaccacgaa gtttcgaacg gattctgttt gctatgcaac cacgggcttg aaggtcaagc
     3601 accttttgtc tgggcaagaa aggacggtgt ggcatttaca atatactgac tggccagatc
     3661 acggctgtcc agaggatgtc caaggatttt tatcctactt ggaggagatc cagtcggtcc
     3721 gtcgccatac caacagcatg ctggaaggca ccaagaaccg gcacccgccc atcgtggtcc
     3781 actgtagtgc tggggtggga aggaccggcg tgctcattct ttctgagctg atgatctact
     3841 gcttggaaca taacgaaaag gtggaagtgc ccatgatgct gaggctcctc agggagcaga
     3901 ggatgttcat gatccagact atcgctcagt acaagtttgt ctaccaagtc ctcatccagt
     3961 tcctccaaaa ctccagactc attttgccaa ctttcttgta caaagtggtt accggttatc
     4021 cgtatgatgt gccggattat gcgtaaacgc gtccggtcca ccaccaccac caccactaag
     4081 gatccggggt tggggttgcg ccttttccaa ggcagccctg ggtttgcgca gggacgcggc
     4141 tgctctgggc gtggttccgg gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc
     4201 acgtccgttc gcagcgtcac ccggatcttc gccgctaccc ttgtgggccc cccggcgacg
     4261 cttcctgctc cgcccctaag tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga
     4321 caaacggaag ccgcacgtct cactagtacc ctcgcagacg gacagcgcca gggagcaatg
     4381 gcagcgcgcc gaccgcgatg ggctgtggcc aatagcggct gctcagcagg gcgcgccgag
     4441 agcagcggcc gggaaggggc ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg
     4501 ttcctgcccg cgcggtgttc cgcattctgc aagcctccgg agcgcacgtc ggcagtcggc
     4561 tccctcgttg accgaatcac cgacctctct ccccagcaat tcaccatgac cgagtacaag
     4621 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     4681 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     4741 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     4801 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     4861 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     4921 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     4981 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     5041 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     5101 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     5161 gaaggaccgc gcacctggtg catgacccgc aagcccggtg ccgaaggtag aggttctctc
     5221 ctcacttgtg gtgatgttga agaaaaccct ggtccaatgt ctagactgga caagagcaaa
     5281 gtcataaacg gagctctgga attactcaat ggtgtcggta tcgaaggcct gacgacaagg
     5341 aaactcgctc aaaagctggg agttgagcag cctaccctgt actggcacgt gaagaacaag
     5401 cgggccctgc tcgatgccct gccaatcgag atgctggaca ggcatcatac ccacttctgc
     5461 cccctggaag gcgagtcatg gcaagacttt ctgcggaaca acgccaagtc ataccgctgt
     5521 gctctcctct cacatcgcga cggggctaaa gtgcatctcg gcacccgccc aacagagaaa
     5581 cagtacgaaa ccctggaaaa tcagctcgcg ttcctgtgtc agcaaggctt ctccctggag
     5641 aacgcactgt acgctctgtc cgccgtgggc cactttacac tgggctgcgt attggaggaa
     5701 caggagcatc aagtagcaaa agaggaaaga gagacaccta ccaccgattc tatgccccca
     5761 cttctgagac aagcaattga gctgttcgac cggcagggag ccgaacctgc cttccttttc
     5821 ggcctggaac taatcatatg tggcctggag aaacagctaa agtgcgaaag cggcgggccg
     5881 accgacgccc ttgacgattt tgacttagac atgctcccag ccgatgccct tgacgacttt
     5941 gaccttgata tgctgcctgc tgacgctctt gacgattttg accttgacat gctccccggg
     6001 taactaagta aggatcgatc caagatatcg tattcttaac tatgttgctc cttttacgct
     6061 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     6121 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     6181 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     6241 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     6301 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     6361 caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc
     6421 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     6481 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     6541 tcagacgagt cggatctccc tttgggccgc ctccccgcct gtttcgcctc ggcgtccgga
     6601 ctagaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     6661 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt
     6721 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     6781 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     6841 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     6901 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
     6961 tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc
     7021 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     7081 tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc
     7141 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     7201 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     7261 gaggcttttt tggaggcctt tcaaatatgt atccgctcat gagacaataa ccctgataaa
     7321 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     7381 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     7441 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     7501 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     7561 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     7621 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     7681 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     7741 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     7801 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     7861 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     7921 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     7981 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     8041 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     8101 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     8161 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     8221 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     8281 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     8341 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     8401 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     8461 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     8521 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     8581 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     8641 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     8701 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     8761 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     8821 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     8881 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     8941 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     9001 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     9061 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     9121 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     9181 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     9241 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     9301 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     9361 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc
     9421 tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac
     9481 gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga
     9541 agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg
     9601 acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat
     9661 aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     9721 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     9781 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     9841 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
     9901 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
     9961 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
    10021 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
    10081 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
    10141 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
    10201 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
    10261 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
    10321 gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg
    10381 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
    10441 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
    10501 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
    10561 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
    10621 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
    10681 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
    10741 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
    10801 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
    10861 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
    10921 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
    10981 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
    11041 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
    11101 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacaattct cgacctcgag
    11161 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
    11221 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
    11281 aattacaaaa attcaaaatt ttc
//
