LOCUS       sequence-438191-       10954 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of an sgRNA with mNeonGreen fluorescent 
            marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10954)
  TITLE     lentiGuide_puro with mNeonGreen marker
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: lentiGuide_puro-P2A-mNeonGreen
FEATURES             Location/Qualifiers
     source          1..10954
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1486..1734
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1486..1506
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     1657..1676
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        3616..3691
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    3747..3864
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4003..5181
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4234..5172
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5129..5149
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             5194..5790
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5194..5213)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5690..5710
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             5800..5856
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5860..6564
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="VSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYEE
                     LNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFEDG
                     ASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIST
                     FKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQK
                     AFTDVMGMDELYK"
     regulatory      6112..6121
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    6580..7168
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6633..6653)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7051..7062)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7240..7473
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7551..7672
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7588..7607)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7642..7661
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7712..7847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7867..7886)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7868..7886)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7896..7913)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7896..7912)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7905..7927)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8054..8509
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8141..8160)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8351..8372
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8535..8639
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8640..8708,8709..9500)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8858..8877)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9671..10259
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10160..10179
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10413..10430
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10547..10568
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10583..10588,10589..10606,10607..10613)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10621..10637
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10626..10648
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10645..10661
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10645..10661
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10680..10700
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10682..10700
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        10728..10954
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccagagaggg cctatttccc
     1501 atgattcctt catatttgca tatacgatac aaggctgtta gagagataat tagaattaat
     1561 ttgactgtaa acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt
     1621 gggtagtttg cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact
     1681 tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaaa caccggagac
     1741 ggttgtaaat gagcacacaa aatacacatg ctaaaatatt atattctatg acctttataa
     1801 aatcaaccaa aatcttcttt ttaataactt tagtatcaat aattagaatt tttatgttcc
     1861 tttttgcaaa cttttaataa aaatgagcaa aataaaaaaa cgctagtttt agtaactcgc
     1921 gttgttttct tcacctttaa taatagctac tccaccactt gttcctaagc ggtcagctcc
     1981 tgcttcaatc attttttgag catcttcaaa tgttctaact ccaccagctg ctttaactaa
     2041 agcattgtct ttaacaactg acttcattag tttaacatct tcaaatgttg cacctgattt
     2101 tgaaaatcct gttgatgttt taacaaattc taatccagct tcaacagcta tttcacaagc
     2161 tttcatgatt tcttcttttg ttaataaaca attttccata atacatttaa caacatgtga
     2221 tccagctgct ttttttacag ctttcatgtc ttctaaaact aattcataat ttttgtcttt
     2281 taatgcacca atatttaata ccatatcaat ttctgttgca ccatctttaa ttgcttcaga
     2341 aacttcgaat gcttttgtag ctgttgtgca tgcacctaga ggaaaaccta caacatttgt
     2401 tattcctaca tttgtgcctt ttaataattc tttacaatag cttgttcaat atgaattaac
     2461 acaaactgtt gcaaaatcaa attcaattgc ttcatcacat aattgtttaa tttcagcttt
     2521 cgtagcatct tgttttaata atgtgtgatc tatatatttg tttagtttca ttttttctcc
     2581 tatatattca tttttaattt taattcttta ataatttcgt ctactttaac tttagcgttt
     2641 tgaacagatt caccaacacc tataaaataa atttttagtt taggttcagt tccacttggg
     2701 cgaacagcaa atcatgactt atcttctaaa taaaatttta gtaagtcttg tcctggcata
     2761 ttatacattc catcgatgta gtcttcaaca ttaacaactt taagtccagc aatttgagtt
     2821 aagggtgttg ctctcaatga tttcattaat ggttcaattt ttaatttctt ttcttctggt
     2881 ttaaaattca agtttaaagt gaaagtgtaa tatgcaccca tttctttaaa taaatcttct
     2941 aaatagtcta ctaatgtttt attttgtttt ttataaaatc aagcagcctc tgctattaat
     3001 atagaagctt gtattccatc tttatctcta gctgagtcat caattacata tccataactt
     3061 tcttcataag caaaaacaaa atttaatccg ttatcttctt ctttagcaat ttctctaccc
     3121 attcatttaa atccagttaa agtttttaca atattaactc catatttttc atgagcgatt
     3181 ctatcaccca aatcacttgt tacaaaactt gaatatagag ccggattttt tggaatgcta
     3241 tttaagcgtt ttagatttga taattttcaa tcaattaaaa ttggtcctgt ttgatttcca
     3301 tctaatctta caaaatgacc atcatgtttt attgccattc caaatctgtc agcatctggg
     3361 tcattcataa taataatatc tgcatcatgt ttaataccat attcaagcgg tatttttcat
     3421 gcaggatcaa attctggatt tggatttaca acatttttaa atgtttcatc ttcaaatgca
     3481 tgctcttcaa cctcaataac gttatatcct gattcacgta atatttttgg ggtaaattta
     3541 gttcctgttc cattaactgc gctaaaaata atttttaaat cttttttagc ttcttgctct
     3601 tttttgtacg tctctgtttt agagctagaa atagcaagtt aaaataaggc tagtccgtta
     3661 tcaacttgaa aaagtggcac cgagtcggtg cttttttaag cttggcgtaa ctagatcttg
     3721 agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
     3781 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
     3841 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccactttgg
     3901 cgccggctcg agggggcccg ggtgcaaaga tggataaagt tttaaacaga gaggaatctt
     3961 tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg tgcccgtcag
     4021 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
     4081 tccggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
     4141 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
     4201 ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg
     4261 cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac ctggctgcag
     4321 tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc
     4381 gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc
     4441 cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc
     4501 atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat
     4561 gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc
     4621 cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc
     4681 ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt
     4741 atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga
     4801 tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag
     4861 cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca
     4921 tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc gagcttttgg
     4981 agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag
     5041 tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc
     5101 ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt
     5161 cttccatttc aggtgtcgtg acgtacggcc accatgaccg agtacaagcc cacggtgcgc
     5221 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac
     5281 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg
     5341 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac
     5401 ggcgccgccg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc
     5461 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg
     5521 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcgga
     5581 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag
     5641 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc
     5701 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc
     5761 acctggtgca tgacccgcaa gcccggtgcc ggatccggcg caacaaactt ctctctgctg
     5821 aaacaagccg gagatgtcga agagaatcct ggaccggtcg tgagcaaggg cgaggaggat
     5881 aacatggcct ctctcccagc gacacatgag ttacacatct ttggctccat caacggtgtg
     5941 gactttgaca tggtgggtca gggcaccggc aatccaaatg atggttatga ggagttaaac
     6001 ctgaagtcca ccaagggtga cctccagttc tccccctgga ttctggtccc tcatatcggg
     6061 tatggcttcc atcagtacct gccctaccct gacgggatgt cgcctttcca ggccgccatg
     6121 gtagatggct ccggatacca agtccatcgc acaatgcagt ttgaagatgg tgcctccctt
     6181 actgttaact accgctacac ctacgaggga agccacatca aaggagaggc ccaggtgaag
     6241 gggactggtt tccctgctga cggtcctgtg atgaccaact cgctgaccgc tgcggactgg
     6301 tgcaggtcga agaagactta ccccaacgac aaaaccatca tcagtacctt taagtggagt
     6361 tacaccactg gaaatggcaa gcgctaccgg agcactgcgc ggaccaccta cacctttgcc
     6421 aagccaatgg cggctaacta tctgaagaac cagccgatgt acgtgttccg taagacggag
     6481 ctcaagcact ccaagaccga gctcaacttc aaggagtggc aaaaggcctt taccgatgtg
     6541 atgggcatgg acgagctgta caagacgcgt taagtcgaca atcaacctct ggattacaaa
     6601 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     6661 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     6721 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     6781 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     6841 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     6901 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     6961 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     7021 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     7081 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     7141 cggatctccc tttgggccgc ctccccgcgt cgactttaag accaatgact tacaaggcag
     7201 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     7261 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     7321 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     7381 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     7441 gaccctttta gtcagtgtgg aaaatctcta gcagtacgta tagtagttca tgtcatctta
     7501 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
     7561 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     7621 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     7681 ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     7741 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     7801 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc
     7861 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt
     7921 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     7981 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     8041 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     8101 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     8161 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     8221 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     8281 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     8341 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     8401 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     8461 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt
     8521 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     8581 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     8641 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     8701 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     8761 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     8821 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     8881 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     8941 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     9001 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     9061 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     9121 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     9181 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     9241 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     9301 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     9361 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     9421 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     9481 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     9541 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     9601 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     9661 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     9721 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     9781 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     9841 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     9901 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     9961 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
    10021 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
    10081 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
    10141 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
    10201 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
    10261 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
    10321 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
    10381 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
    10441 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
    10501 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
    10561 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
    10621 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
    10681 caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc
    10741 aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga
    10801 gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg
    10861 aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga
    10921 tattgtattt aagtgcctag ctcgatacat aaac
//
