LOCUS       sequence-438197-        8375 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CMV promoter drives the expression of an extracellular UDP sensor 
            (UDP1.0) and a membrane localized mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8375)
  AUTHORS   Umpierre AD, Li B, Ayasoufi K, Simon WL, Zhao S, Xie M, Thyen G, Hur
            B, Zheng J, Liang Y, Bosco DB, Maynes MA, Wu Z, Yu X, Sung J, 
            Johnson AJ, Li Y, Wu LJ
  TITLE     Microglial P2Y(6) calcium signaling promotes phagocytosis and shapes
            neuroimmune responses in epileptogenesis.
  JOURNAL   Neuron. 2024 Apr 10:S0896-6273(24)00195-8. doi: 
            10.1016/j.neuron.2024.03.017.
  PUBMED    38614103
REFERENCE   2  (bases 1 to 8375)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2024 Apr 10:S0896-6273(24)00195-8. doi: 
            10.1016/j.neuron.2024.03.017."
COMMENT     Sequence Label: pDisplay-UDP1.0-IRES-mCherry-CAAX
FEATURES             Location/Qualifiers
     source          1..8375
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        10..389
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        390..593
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     544..564
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     638..657
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        638..656
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      719..728
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     sig_peptide     725..784
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     protein_bind    788..812
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     1720..1741
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1847..1868)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2108..2127)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    join(2574..3147,3148..3150,3151..3159)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2741..2758)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2967..2986
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3160..3867
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3305..3323)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3573..3592
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             3925..3954
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3958..4104
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     primer_bind     complement(4126..4143)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4132..4356
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(4488..5076)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4568..4587)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5405..5452
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     5477..5496
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(5684..6478)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTYGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5795..5814)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6405..6424
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     complement(6577..6596)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     enhancer        6675..6866
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     6823..6843
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(join(6926..7717,7718..7786))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7549..7568
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7787..7891)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      7918..8373
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8005..8024)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8215..8236
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gcgcgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
       61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      121 gaccgcccaa cgacccctgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      181 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
      241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc
      601 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga
      661 cccaagcttg gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgc
      721 caccatggag acagacacac tcctgctatg ggtactgctg ctctgggttc caggttccac
      781 tggtgacaca agtttgtaca aaaaagttgg caccagcatg gccaacttca cgggggggag
      841 gaactcgtgc accttccatg aggaattcaa gcaggtcctg ctgcccctgg tctactcagt
      901 ggtgttccta ctggggctgc cactcaatgc cgttgtcatt gggcagatct ggctggcccg
      961 caaggcgttg acccgcacca ccatctacat gctgaacctg gccatggccg acctgcttta
     1021 tgtctgctcc ctccctctcc tcatctacaa ctacacccag aaggattact ggccctttgg
     1081 ggacttcacc tgcaaattcg tccgcttcca gttctacacc aacctgcacg gcagcatcct
     1141 cttcctcacc tgcatcagcg tccagcgcta catggggatc tgccacccct tggcctcgtg
     1201 gcacaaaaag aagggaaaga agctgacgtg gctggtgtgt gctgccgtgt ggttcatcgt
     1261 catcgcccag tgcctgccca cctttgtctt cgcctccacc ggcacgcaga ggaatcgcac
     1321 tgtctgctat gacctgagcc ccccggaccg ctccacatcc tacttcccct atggcatcac
     1381 gttgaccatc accggcttcc tgctgccctt cgcagccatc ctggcctgct actgcagcat
     1441 ggcccgctgg ctgtgcaacc ccaagccggc cgggccgcgc gacaccgacg cgctggacct
     1501 ggaggaggga ggaaacgtct atatcaaggc cgacaagcag aagaacggca tcaaggcgaa
     1561 cttccacatc cgccacaaca tcgaggacgg cggcgtgcag ctcgcctacc actaccagca
     1621 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcgtgca
     1681 gtccaaactt tcgaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     1741 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagggcggta ccggagggag
     1801 catggtgaga aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     1861 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggtgagggcg atgccaccta
     1921 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     1981 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
     2041 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac atccaggagc gcaccatctt
     2101 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     2161 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     2221 caagctggag tacaacaccg gagcagcagc acgctggcgc cacgtgcagg ccgtgcgcat
     2281 gatcatcatc gttgtcatcg tcttctccat cagcttcttc cccttccacc tcaccaagac
     2341 catctacctg atcgtccgct cctcagccag cttgccctgc cctaccctgc aggcttttgc
     2401 cattgcctac aagtgcacgc ggccctttgc cagcatgaac agcgtcctcg accccatcct
     2461 cttctacttc acccagcgca agtttcgtga gagcacccgc tatctcctgg acaagatgag
     2521 ctccaagtgg cggcaagacc actgcatcag ctacggctcc tagtaagcta gcgcccctct
     2581 ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt
     2641 gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct
     2701 ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa
     2761 ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg
     2821 tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc
     2881 caacgccacg tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga
     2941 gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga
     3001 aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tacacatgct
     3061 ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg
     3121 ttttcctttg aaaaacacga tgataatatg gccacaacca tggtgagcaa gggcgaggag
     3181 gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg
     3241 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag
     3301 accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc
     3361 cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac
     3421 ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
     3481 ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg
     3541 aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc
     3601 tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag
     3661 cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag
     3721 gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc
     3781 tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc
     3841 accggcggca tggacgagct gtacaaggat ggtaaaaaga agaaaaagaa gtcaaagaca
     3901 aagtgtgtaa ttatgtaagt cgacgaacaa aaactcatct cagaagagga tctgaatgct
     3961 gtgggccagg acacgcagga ggtcatcgtg gtgccacact ccttgccctt taaggtggtg
     4021 gtgatctcag ccatcctggc cctggtggtg ctcaccatca tctcccttat catcctcatc
     4081 atgctttggc agaagaagcc acgttaggcg gccgctcgag atcagcctcg actgtgcctt
     4141 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     4201 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     4261 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     4321 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagtg
     4381 gcggtaatac ggttatccac agaatcgggg gataacgcag gaaagaacat gtgagcaaaa
     4441 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     4501 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     4561 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     4621 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     4681 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     4741 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     4801 tccaacccgg taagacgcga cttatcgcca ctggcagcag ccactggtaa caggattagc
     4861 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     4921 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     4981 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     5041 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     5101 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     5161 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     5221 atatatgagt aacctgaggc tatggcaggg cctgccgccc cgacgttggc tgcgagccct
     5281 gggccttcac ccgaacttgg ggggtggggt ggggaaaagg aagaaacgcg ggcgtattgg
     5341 ccccaatggg gtctcggtgg ggtatcgaca gagtgccagc cctgggaccg aaccccgcgt
     5401 ttatgaacaa acgacccaac accgtgcgtt ttattctgtc tttttattgc cgtcatagcg
     5461 cgggttcctt ccggtattgt ctccttccgt gtttcagtta gcctccccct agggtgggcg
     5521 aagaactcca gcatgagatc cccgcgctgg aggatcatcc agccggcgtc ccggaaaacg
     5581 attccgaagc ccaacctttc atagaaggcg gcggtggaat cgaaatctcg tgatggcagg
     5641 ttgggcgtcg cttggtcggt catttcgaac cccagagtcc cgctcagaag aactcgtcaa
     5701 gaaggcgata gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa agcacgagga
     5761 agcggtcagc ccattcgccg ccaagctctt cagcaatatc acgggtagcc aacgctatgt
     5821 cctgatagcg gtccgccaca cccagccggc cacagtcgat gaatccagaa aagcggccat
     5881 tttccaccat gatattcggc aagcaggcat cgccataggt cacgacgaga tcctcgccgt
     5941 cgggcatgct cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt
     6001 cgtccagatc atcctgatcg acaagaccgg cttccatccg agtacgtgct cgctcgatgc
     6061 gatgtttcgc ttggtggtcg aatgggcagg tagccggatc aagcgtatgc agccgccgca
     6121 ttgcatcagc catgatggat actttctcgg caggagcaag gtgagatgac aggagatcct
     6181 gccccggcac ttcgcccaat agcagccagt cccttcccgc ttcagtgaca acgtcgagca
     6241 cagctgcgca aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc tcgtcttgca
     6301 gttcattcag ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg
     6361 acagccggaa cacggcggca tcagagcagc cgattgtctg ttgtgcccag tcatagccga
     6421 atagcctctc cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt tcaatcatgc
     6481 gaaacgatcc tcatcctgtc tcttgatcga tctttgcaaa agcctaggcc tccaaaaaag
     6541 cctcctcact acttctggaa tagctcagag gccgggcctc ggcctctgca taaataaaaa
     6601 aaattagtca gccatggggc ggagaatggg cggaactggg cggagttagg ggcggaatgg
     6661 gcggagttag gggcgggact atggttgctg actaattgag atgcatgctt tgcatacttc
     6721 tgcctgctgg ggagcctggg gactttccac acctggttgc tgactaattg agatgcatgc
     6781 tttgcatact tctgcctgct ggggagcctg gggactttcc acaccctaac tgacacacat
     6841 tccacagctg gttctttccg cctcaggact cttccttttt caataaatca atctaaagta
     6901 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     6961 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     7021 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     7081 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     7141 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     7201 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     7261 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     7321 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     7381 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     7441 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     7501 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     7561 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     7621 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     7681 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     7741 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     7801 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     7861 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     7921 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     7981 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     8041 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     8101 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     8161 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     8221 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     8281 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     8341 cgaattttaa caaaatatta acgcttacaa tttac
//
