LOCUS       sequence-438198-       16082 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-compatible Level M for expression nnLuz, nnH3H, nnCPH in 
            plants (N.benthamiana, BY2 cells).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16082)
  AUTHORS   Palkina KA, Karataeva TA, Perfilov MM, Fakhranurova LI, Markina NM, 
            Somermeyer LG, Garcia-Perez E, Vazquez-Vilar M, Rodriguez-Rodriguez 
            M, Vazquez-Vilriales V, Shakhova ES, Mitiouchkina T, Belozerova OA, 
            Kovalchuk SI, Alekberova A, Malyshevskaia AK, Bugaeva EN, Guglya EB,
            Balakireva A, Sytov N, Bezlikhotnova A, Boldyreva DI, Babenko VV, 
            Kondrashov FA, Choob VV, Orzaez D, Yampolsky IV, Mishin AS, 
            Sarkisyan KS
  TITLE     A hybrid pathway for self-sustained luminescence.
  JOURNAL   Sci Adv. 2024 Mar 8;10(10):eadk1992. doi: 10.1126/sciadv.adk1992. 
            Epub 2024 Mar 8.
  PUBMED    38457503
REFERENCE   2  (bases 1 to 16082)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2024-03-8"; volume: "10(10):eadk1992. doi"; pages: " 
            10.1126/sciadv.adk1992. Epub 2024 Mar 8"
COMMENT     Sequence Label: pNK7190
FEATURES             Location/Qualifiers
     source          1..16082
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    26..50
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      138..848
                     /label=oriV
                     /note="incP origin of replication"
     CDS             1143..1934
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2275..2863
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2764..2783
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             3199..4347
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    4493..4517
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        4689..4868
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    4899..4953
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             4955..5749
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MVEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5009..5028)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5619..5638
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      5776..6483
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     promoter        7495..7840
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      9206..9913
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     promoter        10930..11275
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      12293..13000
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     promoter        14017..14362
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             complement(14923..14934)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      15327..16034
                     /label=OCS terminator
                     /note="octopine synthase terminator"
ORIGIN
        1 gtgatccgtt taaactatca gtgtttgaca ggatatattg gcgggtaaac ctaagagaaa
       61 agagcgttta ttagaataat cggatattta aaagggcgtg aaaaggttta tccgttcgtc
      121 catttgtatg tgccagccgc ctttgcgacg ctcaccgggc tggttgccct cgccgctggg
      181 ctggcggccg tctatggccc tgcaaacgcg ccagaaacgc cgtcgaagcc gtgtgcgaga
      241 caccgcggcc gccggcgttg tggatacctc gcggaaaact tggccctcac tgacagatga
      301 ggggcggacg ttgacacttg aggggccgac tcacccggcg cggcgttgac agatgagggg
      361 caggctcgat ttcggccggc gacgtggagc tggccagcct cgcaaatcgg cgaaaacgcc
      421 tgattttacg cgagtttccc acagatgatg tggacaagcc tggggataag tgccctgcgg
      481 tattgacact tgaggggcgc gactactgac agatgagggg cgcgatcctt gacacttgag
      541 gggcagagtg ctgacagatg aggggcgcac ctattgacat ttgaggggct gtccacaggc
      601 agaaaatcca gcatttgcaa gggtttccgc ccgtttttcg gccaccgcta acctgtcttt
      661 taacctgctt ttaaaccaat atttataaac cttgttttta accagggctg cgccctgtgc
      721 gcgtgaccgc gcacgccgaa ggggggtgcc cccccttctc gaaccctccc ggcccgctaa
      781 cgcgggcctc ccatcccccc aggggctgcg cccctcggcc gcgaacggcc tcaccccaaa
      841 aatggcagcg ctggccaatt cccgaggcac gaacccagtg gacataagcc tgttcggttc
      901 gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac
      961 gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg
     1021 tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga
     1081 tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca
     1141 tcatggggga agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca
     1201 tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg
     1261 gcggcctgaa gccacacagc gatattgatt tgctggttac ggtgaccgta aggcttgatg
     1321 aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga
     1381 gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc
     1441 gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag
     1501 gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag
     1561 aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac
     1621 aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg
     1681 ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg
     1741 gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt
     1801 atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg
     1861 cctcgcgcgc agatcagttg gaagaatttg tccattacgt gaaaggcgag atcaccaagg
     1921 tagtcggcaa ataatgtcta gctagaaatt cgttcaagcc gacgccgctt cgcggcgcgg
     1981 cttaactcaa gcgttagatg cactaagcac ataattgctc acagccaaac tatcaggtca
     2041 agtctgcttt tattattttt aagcgtgcat aataagccct acacaaattg ggagatatat
     2101 catgctgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     2161 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     2221 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     2281 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     2341 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     2401 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     2461 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     2521 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     2581 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     2641 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     2701 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     2761 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     2821 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     2881 ctttttacgg ttcctggcag atcctagatg tggcgcaacg atgccggcga caagcaggag
     2941 cgcaccgact tcttccgcat caagtgtttt ggctctcagg ccgaggccca cggcaagtat
     3001 ttgggcaagg ggtcgctggt attcgtgcag ggcaagattc ggaataccaa gtacgagaag
     3061 gacggccaga cggtctacgg gaccgacttc attgccgata aggtggatta tctggacacc
     3121 aaggcaccag gcgggtcaaa tcaggaataa gggcacattg ccccggcgtg agtcggggca
     3181 atcccgcaag gagggtgaat gaatcggacg tttgaccgga aggcatacag gcaagaactg
     3241 atcgacgcgg ggttttccgc cgaggatgcc gaaaccatcg caagccgcac cgtcatgcgt
     3301 gcgccccgcg aaaccttcca gtccgtcggc tcgatggtcc agcaagctac ggccaagatc
     3361 gagcgcgaca gcgtgcaact ggctccccct gccctgcccg cgccatcggc cgccgtggag
     3421 cgttcgcgtc gtcttgaaca ggaggcggca ggtttggcga agtcgatgac catcgacacg
     3481 cgaggaacta tgacgaccaa gaagcgaaaa accgccggcg aggacctggc aaaacaggtc
     3541 agcgaggcca agcaggccgc gttgctgaaa cacacgaagc agcagatcaa ggaaatgcag
     3601 ctttccttgt tcgatattgc gccgtggccg gacacgatgc gagcgatgcc aaacgacacg
     3661 gcccgctctg ccctgttcac cacgcgcaac aagaaaatcc cgcgcgaggc gctgcaaaac
     3721 aaggtcattt tccacgtcaa caaggacgtg aagatcacct acaccggcgt cgagctgcgg
     3781 gccgacgatg acgaactggt gtggcagcag gtgttggagt acgcgaagcg cacccctatc
     3841 ggcgagccga tcaccttcac gttctacgag ctttgccagg acctgggctg gtcgatcaat
     3901 ggccggtatt acacgaaggc cgaggaatgc ctgtcgcgcc tacaggcgac ggcgatgggc
     3961 ttcacgtccg accgcgttgg gcacctggaa tcggtgtcgc tgctgcaccg cttccgcgtc
     4021 ctggaccgtg gcaagaaaac gtcccgttgc caggtcctga tcgacgagga aatcgtcgtg
     4081 ctgtttgctg gcgaccacta cacgaaattc atatgggaga agtaccgcaa gctgtcgccg
     4141 acggcccgac ggatgttcga ctatttcagc tcgcaccggg agccgtaccc gctcaagctg
     4201 gaaaccttcc gcctcatgtg cggatcggat tccacccgcg tgaagaagtg gcgcgagcag
     4261 gtcggcgaag cctgcgaaga gttgcgaggc agcggcctgg tggaacacgc ctgggtcaat
     4321 gatgacctgg tgcattgcaa acgctagggc cttgtggggt cagttccggc tgggggttca
     4381 gcagcccctg ctcggatctg ttggaccgga cagtagtcat ggttgatggg ctgcctgtat
     4441 cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct ggtggcagga
     4501 tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg cggacgtttt
     4561 taatgtactg gggttgaaca ctctgtgggt ctcatgccga attcggatcc ggagcggaga
     4621 attaagggag tcacgttatg acccccgccg atgacgcggg acaagccgtt ttacgtttgg
     4681 aactgacaga accgcaacgt tgaaggagcc actgagccgc gggtttctgg agtttaatga
     4741 gctaagcaca tacgtcagaa accattattg cgcgttcaaa agtcgcctaa ggtcactatc
     4801 agctagcaaa tatttcttgt caaaaatgct ccactgacgt tccataaatt cccctcggta
     4861 tccaattaga gtctcatatt cactctccta tttttacaac aattaccaac aacaacaaac
     4921 aacaaacaac attacaatta catttacaat taccatggtt gaacaagatg gattgcacgc
     4981 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
     5041 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
     5101 caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg
     5161 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
     5221 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
     5281 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
     5341 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
     5401 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
     5461 actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgactcatgg
     5521 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
     5581 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
     5641 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
     5701 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg
     5761 gggttcgcta gagtcctgct ttaatgagat atgcgagacg cctatgatcg catgatattt
     5821 gctttcaatt ctgttgtgca cgttgtaaaa aacctgagca tgtgtagctc agatccttac
     5881 cgccggtttc ggttcattct aatgaatata tcacccgtta ctatcgtatt tttatgaata
     5941 atattctccg ttcaatttac tgattgtacc ctactactta tatgtacaat attaaaatga
     6001 aaacaatata ttgtgctgaa taggtttata gcgacatcta tgatagagcg ccacaataac
     6061 aaacaattgc gttttattat tacaaatcca attttaaaaa aagcggcaga accggtcaaa
     6121 cctaaaagac tgattacata aatcttattc aaatttcaaa agtgccccag gggctagtat
     6181 ctacgacaca ccgagcggcg aactaataac gctcactgaa gggaactccg gttccccgcc
     6241 ggcgcgcatg ggtgagattc cttgaagttg agtattggcc gtccgctcta ccgaaagtta
     6301 cgggcaccat tcaacccggt ccagcacggc ggccgggtaa ccgacttgct gccccgagaa
     6361 ttatgcagca tttttttggt gtatgtgggc cccaaatgaa gtgcaggtca aaccttgaca
     6421 gtgacgacaa atcgttgggc gggtccaggg cgaattttgc gacaacatgt cgaggctcag
     6481 ccgctgcaag aattcaagct tggaggaatt ccaatcccac aaaaatctga gcttaacagc
     6541 acagttgctc ctctcagagc agaatcgggt attcaacacc ctcatatcaa ctactacgtt
     6601 gtgtataacg gtccacatgc cggtatatac gatgactggg gttgtacaaa ggcggcaaca
     6661 aacggcgttc ccggagttgc acacaagaaa tttgccacta ttacagaggc aagagcagca
     6721 gctgacgcgt acacaacaag tcagcaaaca gacaggttga acttcatccc caaaggagaa
     6781 gctcaactca agcccaagag ctttgctaag gccctaacaa gcccaccaaa gcaaaaagcc
     6841 cactggctca cgctaggaac caaaaggccc agcagtgatc cagccccaaa agagatctcc
     6901 tttgccccgg agattacaat ggacgatttc ctctatcttt acgatctagg aaggaagttc
     6961 gaaggtgaag gtgacgacac tatgttcacc actgataatg agaaggttag cctcttcaat
     7021 ttcagaaaga atgctgaccc acagatggtt agagaggcct acgcagcaag tctcatcaag
     7081 acgatctacc cgagtaacaa tctccaggag atcaaatacc ttcccaagaa ggttaaagat
     7141 gcagtcaaaa gattcaggac taattgcatc aagaacacag agaaagacat atttctcaag
     7201 atcagaagta ctattccagt atggacgatt caaggcttgc ttcataaacc aaggcaagta
     7261 atagagattg gagtctctaa aaaggtagtt cctactgaat ctaaggccat gcatggagtc
     7321 taagattcaa atcgaggatc taacagaact cgccgtcaag actggcgaac agttcataca
     7381 gagtctttta cgactcaatg acaagaagaa aatcttcgtc aacatggtgg agcacgacac
     7441 tctggtctac tccaaaaatg tcaaagatac agtctcagaa gatcaaaggg ctattgagac
     7501 ttttcaacaa aggataattt cgggaaacct cctcggattc cattgcccag ctatctgtca
     7561 cttcatcgaa aggacagtag aaaaggaagg tggctcctac aaatgccatc attgcgataa
     7621 aggaaaggct atcattcaag atctctctgc cgacagtggt cccaaagatg gacccccacc
     7681 cacgaggagc atcgtggaaa aagaagaggt tccaaccacg tctacaaagc aagtggattg
     7741 atgtgacatc tccactgacg taagggatga cgcacaatcc cactatcctt cgcaagaccc
     7801 ttcctctata taaggaagtt catttcattt ggagaggaca cgctcgagta taagagctca
     7861 tttttacaac aattaccaac aacaacaaac aacaaacaac attacaatta catttacaat
     7921 tatcgataca atggcctctt ttgaaaattc actctctgtg ttgatcgtgg gagccggttt
     7981 gggtggtcta gcagctgcta tcgctctccg acgacagggc cacgtggtta agatctacga
     8041 ctcatctagc tttaaggccg agctcggcgc aggcttggca gttccaccga atacactgcg
     8101 tagtctacag caattgggtt gtaacactga aaaccttaac ggcgttgata atctctgttt
     8161 tactgccatg ggatatgatg ggtcagtggg catgatgaac aatatgactg attaccgtga
     8221 agcatatggc actagttgga tcatggttca tcgtgtcgat ttgcataacg agttgatgag
     8281 agttgctttg gacccagggg gattgggccc tcccgcaacg cttcacctga atcatcgagt
     8341 cacgttttgt gatgtagatg cttgtacggt gacatttacc aacggtacaa ctcaaagtgc
     8401 agatcttatc gttggggctg acgggatacg ttcaactatc cgtagatttg tcttggagga
     8461 ggacgtgacc gttcccgcaa gcggaattgt ggggtttcga tggttggtac aggctgatgc
     8521 cctcgatcca tatcctgaac tcgattggat agtcaaaaag ccaccgctcg gagctaggtt
     8581 gatttctacg ccccagaatc cacaatctgg agtgggactg gcagatcgta gaactattat
     8641 tatatacgct tgtcgtgggg ggacaatggt aaatgtgctg gccgtacacg atgacgagcg
     8701 agatcagaat actgcagatt ggagcgttcc agcatcaaag gatgacttgt ttcgagtatt
     8761 ccatgactac catccgagat tcagacggct cttggaattg gctcaagata taaatctctg
     8821 gcagatgagg gttgtgccgg tgctcaagaa atgggtgaac aaacgagtgt gcctgcttgg
     8881 agatgccgct catgcctctc ttccgactct cggccagggt ttcgggatgg gattggagga
     8941 cgccgtagcc cttggtactc tcttgccaaa aggtacgact gctagtcaaa tagagacaag
     9001 actggctgtc tacgaacagc ttagaaagga ccgagcagag tttgtagccg ctgaaagtta
     9061 tgaagagcag tatgtccccg agatgagggg cttgtacttg cgtagcaagg aacttagaga
     9121 tagagtcatg gggtatgaca taaaagtgga gtctgagaag gtactggaaa ccttgcttag
     9181 gagctctaac tcagcttagc ttgtcctgct ttaatgagat atgcgagaag cctatgatcg
     9241 catgatattt gctttcaatt ctgttgtgca cgttgtaaaa aacctgagca tgtgtagctc
     9301 agatccttac cgccggtttc ggttcattct aatgaatata tcacccgtta ctatcgtatt
     9361 tttatgaata atattctccg ttcaatttac tgattgtacc ctactactta tatgtacaat
     9421 attaaaatga aaacaatata ttgtgctgaa taggtttata gcgacatcta tgatagagcg
     9481 ccacaataac aaacaattgc gttttattat tacaaatcca attttaaaaa aagcggcaga
     9541 accggtcaaa cctaaaagac tgattacata aatcttattc aaatttcaaa agtgccccag
     9601 gggctagtat ctacgacaca ccgagcggcg aactaataac gctcactgaa gggaactccg
     9661 gttccccgcc ggcgcgcatg ggtgagattc cttgaagttg agtattggcc gtccgctcta
     9721 ccgaaagtta cgggcaccat tcaacccggt ccagcacggc ggccgggtaa ccgacttgct
     9781 gccccgagaa ttatgcagca tttttttggt gtatgtgggc cccaaatgaa gtgcaggtca
     9841 aaccttgaca gtgacgacaa atcgttgggc gggtccaggg cgaattttgc gacaacatgt
     9901 cgaggctcag caggaccgct actagaattc gagctcggag gaattccaat cccacaaaaa
     9961 tctgagctta acagcacagt tgctcctctc agagcagaat cgggtattca acaccctcat
    10021 atcaactact acgttgtgta taacggtcca catgccggta tatacgatga ctggggttgt
    10081 acaaaggcgg caacaaacgg cgttcccgga gttgcacaca agaaatttgc cactattaca
    10141 gaggcaagag cagcagctga cgcgtacaca acaagtcagc aaacagacag gttgaacttc
    10201 atccccaaag gagaagctca actcaagccc aagagctttg ctaaggccct aacaagccca
    10261 ccaaagcaaa aagcccactg gctcacgcta ggaaccaaaa ggcccagcag tgatccagcc
    10321 ccaaaagaga tctcctttgc cccggagatt acaatggacg atttcctcta tctttacgat
    10381 ctaggaagga agttcgaagg tgaaggtgac gacactatgt tcaccactga taatgagaag
    10441 gttagcctct tcaatttcag aaagaatgct gacccacaga tggttagaga ggcctacgca
    10501 gcaagtctca tcaagacgat ctacccgagt aacaatctcc aggagatcaa ataccttccc
    10561 aagaaggtta aagatgcagt caaaagattc aggactaatt gcatcaagaa cacagagaaa
    10621 gacatatttc tcaagatcag aagtactatt ccagtatgga cgattcaagg cttgcttcat
    10681 aaaccaaggc aagtaataga gattggagtc tctaaaaagg tagttcctac tgaatctaag
    10741 gccatgcatg gagtctaaga ttcaaatcga ggatctaaca gaactcgccg tcaagactgg
    10801 cgaacagttc atacagagtc ttttacgact caatgacaag aagaaaatct tcgtcaacat
    10861 ggtggagcac gacactctgg tctactccaa aaatgtcaaa gatacagtct cagaagatca
    10921 aagggctatt gagacttttc aacaaaggat aatttcggga aacctcctcg gattccattg
    10981 cccagctatc tgtcacttca tcgaaaggac agtagaaaag gaaggtggct cctacaaatg
    11041 ccatcattgc gataaaggaa aggctatcat tcaagatctc tctgccgaca gtggtcccaa
    11101 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gaggttccaa ccacgtctac
    11161 aaagcaagtg gattgatgtg acatctccac tgacgtaagg gatgacgcac aatcccacta
    11221 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga ggacacgctc
    11281 gagtataaga gctcattttt acaacaatta ccaacaacaa caaacaacaa acaacattac
    11341 aattacattt acaattatcg atacaatggc acctatttca tccacgtgga gcaggttgat
    11401 aaggttcgtg gctgtagaaa cttccctggt tcatattggt gagccaattg atgcaacgat
    11461 ggacgttggc ctcgcccgta gagaaggtaa gacaattcaa gcatacgaaa taatcggaag
    11521 cggcagtgcc ctcgatcttt ctgcacaagt ctctaagaac gtactcacag ttagggaatt
    11581 gttgatgccg ctcagtcgag aagagattaa gaccgttcga tgcctcggct tgaactatcc
    11641 ggtccacgcc acagaagcta acgtggccgt gccgaagttt cctaacctgt tctataagcc
    11701 cgtaacatca ttgattggtc caggcggtct tattactatt ccctccgtcg ttcaaccccc
    11761 caaggagcac cagtcagatt acgaggctga gctggttata gtaataggta aggcagcaaa
    11821 gaacgtatca gaggatgagg ctttggatta tgtattgggc tataccgctg caaacgatat
    11881 atctttccgt aagcatcaat tggctgtcag tcaatggagc ttcagcaagg gctttgatgg
    11941 aactaatcca cttggtccct gcttggtttc cgcctccagt atccctgacc cacaggatat
    12001 tccaatacag tgcaaattga atggtggagt cgtccaaaat ggcaacacca gagatcaaat
    12061 cttcaacgtt aagaaaacca tatcatttct ttcccagggg actactttgg agcccggttc
    12121 cattatcctt accgggaccc cagatggggt ggggttcgta cgaaacccgc ccctgtattt
    12181 gaaggatggc gatgaggtta tgacttggat cggtagtgga atcggaactc tcgcaaatac
    12241 agttcgagaa gaacaaacgt gctttgcatc aggcggccac gaatagcttg tcctgcttta
    12301 atgagatatg cgagaagcct atgatcgcat gatatttgct ttcaattctg ttgtgcacgt
    12361 tgtaaaaaac ctgagcatgt gtagctcaga tccttaccgc cggtttcggt tcattctaat
    12421 gaatatatca cccgttacta tcgtattttt atgaataata ttctccgttc aatttactga
    12481 ttgtacccta ctacttatat gtacaatatt aaaatgaaaa caatatattg tgctgaatag
    12541 gtttatagcg acatctatga tagagcgcca caataacaaa caattgcgtt ttattattac
    12601 aaatccaatt ttaaaaaaag cggcagaacc ggtcaaacct aaaagactga ttacataaat
    12661 cttattcaaa tttcaaaagt gccccagggg ctagtatcta cgacacaccg agcggcgaac
    12721 taataacgct cactgaaggg aactccggtt ccccgccggc gcgcatgggt gagattcctt
    12781 gaagttgagt attggccgtc cgctctaccg aaagttacgg gcaccattca acccggtcca
    12841 gcacggcggc cgggtaaccg acttgctgcc ccgagaatta tgcagcattt ttttggtgta
    12901 tgtgggcccc aaatgaagtg caggtcaaac cttgacagtg acgacaaatc gttgggcggg
    12961 tccagggcga attttgcgac aacatgtcga ggctcagcag gaccgcttta cgaattccca
    13021 tggggaggaa ttccaatccc acaaaaatct gagcttaaca gcacagttgc tcctctcaga
    13081 gcagaatcgg gtattcaaca ccctcatatc aactactacg ttgtgtataa cggtccacat
    13141 gccggtatat acgatgactg gggttgtaca aaggcggcaa caaacggcgt tcccggagtt
    13201 gcacacaaga aatttgccac tattacagag gcaagagcag cagctgacgc gtacacaaca
    13261 agtcagcaaa cagacaggtt gaacttcatc cccaaaggag aagctcaact caagcccaag
    13321 agctttgcta aggccctaac aagcccacca aagcaaaaag cccactggct cacgctagga
    13381 accaaaaggc ccagcagtga tccagcccca aaagagatct cctttgcccc ggagattaca
    13441 atggacgatt tcctctatct ttacgatcta ggaaggaagt tcgaaggtga aggtgacgac
    13501 actatgttca ccactgataa tgagaaggtt agcctcttca atttcagaaa gaatgctgac
    13561 ccacagatgg ttagagaggc ctacgcagca agtctcatca agacgatcta cccgagtaac
    13621 aatctccagg agatcaaata ccttcccaag aaggttaaag atgcagtcaa aagattcagg
    13681 actaattgca tcaagaacac agagaaagac atatttctca agatcagaag tactattcca
    13741 gtatggacga ttcaaggctt gcttcataaa ccaaggcaag taatagagat tggagtctct
    13801 aaaaaggtag ttcctactga atctaaggcc atgcatggag tctaagattc aaatcgagga
    13861 tctaacagaa ctcgccgtca agactggcga acagttcata cagagtcttt tacgactcaa
    13921 tgacaagaag aaaatcttcg tcaacatggt ggagcacgac actctggtct actccaaaaa
    13981 tgtcaaagat acagtctcag aagatcaaag ggctattgag acttttcaac aaaggataat
    14041 ttcgggaaac ctcctcggat tccattgccc agctatctgt cacttcatcg aaaggacagt
    14101 agaaaaggaa ggtggctcct acaaatgcca tcattgcgat aaaggaaagg ctatcattca
    14161 agatctctct gccgacagtg gtcccaaaga tggaccccca cccacgagga gcatcgtgga
    14221 aaaagaagag gttccaacca cgtctacaaa gcaagtggat tgatgtgaca tctccactga
    14281 cgtaagggat gacgcacaat cccactatcc ttcgcaagac ccttcctcta tataaggaag
    14341 ttcatttcat ttggagagga cacgctcgag tataagagct catttttaca acaattacca
    14401 acaacaacaa acaacaaaca acattacaat tacatttaca attatcgata caatgaggat
    14461 aaatatctct ttgtctagtc tctttgagag actgagcaaa ttgtcatccc gatccatcgc
    14521 aattacctgc ggcgtagtct tggcaagtgc aatagcattc ccaattatcc gaagagacta
    14581 tcaaacgttc cttgaagtcg gtccgagcta tgccccacag aacttccgag gctatatcat
    14641 cgtttgtgtc ttgtcacttt ttaggcaaga gcagaaaggg ttggcaatct atgacaggct
    14701 tccagaaaag aggcgttggc ttgccgactt gccgtttcgt gaagggacga ggccatccat
    14761 aacctcccac attatacagc gtcagcgtac tcagctggta gatcaagagt ttgcaaccag
    14821 agaacttatc gataaggtga tcccacgtgt gcaagcaaga cacacagaca aaacttttct
    14881 cagtacgtca aaatttgagt ttcatgcaaa ggccatcttc cttctcccct ctatccctat
    14941 taatgatcct cttaacattc cctcacacga tacggtaaga agaaccaagc gtgagatcgc
    15001 tcacatgcat gattaccatg attgcactct gcacttggct cttgctgctc aggatggtaa
    15061 ggaagttttg aagaaggggt ggggccagcg tcacccactg gccggaccag gagtcccagg
    15121 ccctcctact gagtggacct tcctttacgc accaaggaac gaagaggagg ccagagttgt
    15181 cgagatgata gtcgaagcta gtattggcta catgactaac gatcctgctg gtaagattgt
    15241 cgaaaatgcc aagtcaggtt agtaatgaat ggctgacgag tgaatcaact aaccccttgc
    15301 caatactgcc taataataag cttgtcctgc tttaatgaga tatgcgagaa gcctatgatc
    15361 gcatgatatt tgctttcaat tctgttgtgc acgttgtaaa aaacctgagc atgtgtagct
    15421 cagatcctta ccgccggttt cggttcattc taatgaatat atcacccgtt actatcgtat
    15481 ttttatgaat aatattctcc gttcaattta ctgattgtac cctactactt atatgtacaa
    15541 tattaaaatg aaaacaatat attgtgctga ataggtttat agcgacatct atgatagagc
    15601 gccacaataa caaacaattg cgttttatta ttacaaatcc aattttaaaa aaagcggcag
    15661 aaccggtcaa acctaaaaga ctgattacat aaatcttatt caaatttcaa aagtgcccca
    15721 ggggctagta tctacgacac accgagcggc gaactaataa cgctcactga agggaactcc
    15781 ggttccccgc cggcgcgcat gggtgagatt ccttgaagtt gagtattggc cgtccgctct
    15841 accgaaagtt acgggcacca ttcaacccgg tccagcacgg cggccgggta accgacttgc
    15901 tgccccgaga attatgcagc atttttttgg tgtatgtggg ccccaaatga agtgcaggtc
    15961 aaaccttgac agtgacgaca aatcgttggg cgggtccagg gcgaattttg cgacaacatg
    16021 tcgaggctca gcaggaccgc tcagatgaga ccgaggatgc acatgtgacc gagggacacg
    16081 aa
//
