LOCUS       sequence-438199-        3079 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-compatible Level 0 vector carrying promoter pORCA3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Balakireva AV, Karataeva TA, Karampelias M, Mitiouchkina TY, 
            Machacek J, Shakhova ES, Perfilov MM, Belozerova OA, Palkina KA, 
            Drazna N, Vondrakova Z, Fleiss A, Fakhranurova LI, Markina NM, 
            Morozov VV, Bugaeva EN, Delnova GM, Choob VV, Yampolsky IV, Petrasek
            J, Mishin AS, Sarkisyan KS
  TITLE     Non-invasive imaging of salicylic and jasmonic acid activities in 
            planta
  JOURNAL   bioRxiv 2025.02.17.636591; doi: 
            https://doi.org/10.1101/2025.02.17.636591
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.02.17.636591; doi: https://doi.org/10.1101/2025.02.17.636591"
COMMENT     Sequence Label: M6570
FEATURES             Location/Qualifiers
     source          1..3079
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      847..1435
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1336..1355
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1589..1606
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2552..2570)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2638..2742
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2964..3079,1..676)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 tggcggcctg aagccacaca gcgatattga tttgctggtt acggtgaccg taaggcttga
       61 tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga
      121 gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg
      181 gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc
      241 aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag
      301 agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga
      361 acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg
      421 ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac
      481 cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca
      541 gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt
      601 ggcctcgcgc gcagatcagt tggaagaatt tgtccattac gtaaaaggcg agatcaccaa
      661 ggtagtcggc aaataactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
      721 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
      781 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
      841 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
      901 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
      961 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     1021 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     1081 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     1141 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     1201 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     1261 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     1321 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     1381 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     1441 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     1501 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     1561 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     1621 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatcac tctgtggtct
     1681 caggaggaat tcttaccatg tcccaaataa ataaaaacgg agagtaaaaa ctatactaaa
     1741 tgcttaggag cagaaaaaat aaatgttgga taagatgaac gaatcaatta ttatgtaaat
     1801 gtggtggcta gtttcaaaaa aatactttat catcatcacg gtatggactt cgtcagtatt
     1861 catcaatgcc aactttttct ttccgtccaa aatgtataat tgttttaaaa tacactatct
     1921 aaacatttaa gtcatatttt tttacgtgag taaggatatt tctgtctcac gtcatgcgta
     1981 atttcatcat tgtctgttga atacttttat tcgtgttaga gggtggctca aatgtgtaga
     2041 tgactgtatt tatattaatc tttgatattt cttttatatt tttatctcaa aattgattga
     2101 atcgtgtata taatcttcaa aattattttt ttttaacttc aaaaaatttt caaattttaa
     2161 aaaattacca cattttttgg aaagaaagta aaaacaacat gtatagagac agaaattttt
     2221 ttctgatata aattttatat tcttttacgt aatttatcgg cccaaaataa tttacttaag
     2281 aaatacgtgt tccataatta tttaattctg tgtaaacgtg actaattgca cctcccaagc
     2341 gccatcgctg gaccttcaag gaacactcta caaattattt ttcaataaaa tatttatcca
     2401 tttttcaaac gtgccttttt acctcttagt atataaattc cactcctcac tctattcatc
     2461 actccaaatc caaaaaactt cataaaaaat cagtttctaa aaaagaagaa aaaatgtgag
     2521 accacgaagt ggctcttcag tggacgaaag ggcctcgtga tacgcctatt tttataggtt
     2581 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     2641 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     2701 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgcgctc acgcaactgg
     2761 tccagaacct tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt
     2821 tatgactgtt tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt
     2881 acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc
     2941 gccctaaaac aaagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac
     3001 tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt
     3061 tgtacggctc cgcagtgga
//
